Job ID = 11371343 sra ファイルのダウンロード中... Completed: 1975012K bytes transferred in 35 seconds (450966K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 51831789 spots for /home/okishinya/chipatlas/results/dm3/SRX4580637/SRR7724142.sra Written 51831789 spots for /home/okishinya/chipatlas/results/dm3/SRX4580637/SRR7724142.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:16:16 51831789 reads; of these: 51831789 (100.00%) were unpaired; of these: 4378904 (8.45%) aligned 0 times 37590328 (72.52%) aligned exactly 1 time 9862557 (19.03%) aligned >1 times 91.55% overall alignment rate Time searching: 00:16:16 Overall time: 00:16:16 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 5585348 / 47452885 = 0.1177 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Thu, 06 Dec 2018 03:19:10: # Command line: callpeak -t SRX4580637.bam -f BAM -g dm -n SRX4580637.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX4580637.10 # format = BAM # ChIP-seq file = ['SRX4580637.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 06 Dec 2018 03:19:10: # Command line: callpeak -t SRX4580637.bam -f BAM -g dm -n SRX4580637.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX4580637.20 # format = BAM # ChIP-seq file = ['SRX4580637.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 06 Dec 2018 03:19:10: #1 read tag files... INFO @ Thu, 06 Dec 2018 03:19:10: #1 read tag files... INFO @ Thu, 06 Dec 2018 03:19:10: #1 read treatment tags... INFO @ Thu, 06 Dec 2018 03:19:10: #1 read treatment tags... INFO @ Thu, 06 Dec 2018 03:19:10: # Command line: callpeak -t SRX4580637.bam -f BAM -g dm -n SRX4580637.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX4580637.05 # format = BAM # ChIP-seq file = ['SRX4580637.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 06 Dec 2018 03:19:10: #1 read tag files... INFO @ Thu, 06 Dec 2018 03:19:10: #1 read treatment tags... INFO @ Thu, 06 Dec 2018 03:19:16: 1000000 INFO @ Thu, 06 Dec 2018 03:19:16: 1000000 INFO @ Thu, 06 Dec 2018 03:19:19: 1000000 INFO @ Thu, 06 Dec 2018 03:19:23: 2000000 INFO @ Thu, 06 Dec 2018 03:19:23: 2000000 INFO @ Thu, 06 Dec 2018 03:19:27: 2000000 INFO @ Thu, 06 Dec 2018 03:19:29: 3000000 INFO @ Thu, 06 Dec 2018 03:19:29: 3000000 INFO @ Thu, 06 Dec 2018 03:19:36: 3000000 INFO @ Thu, 06 Dec 2018 03:19:37: 4000000 INFO @ Thu, 06 Dec 2018 03:19:38: 4000000 INFO @ Thu, 06 Dec 2018 03:19:44: 5000000 INFO @ Thu, 06 Dec 2018 03:19:46: 4000000 INFO @ Thu, 06 Dec 2018 03:19:47: 5000000 INFO @ Thu, 06 Dec 2018 03:19:51: 6000000 INFO @ Thu, 06 Dec 2018 03:19:55: 5000000 INFO @ Thu, 06 Dec 2018 03:19:57: 6000000 INFO @ Thu, 06 Dec 2018 03:19:59: 7000000 INFO @ Thu, 06 Dec 2018 03:20:04: 6000000 INFO @ Thu, 06 Dec 2018 03:20:06: 7000000 INFO @ Thu, 06 Dec 2018 03:20:07: 8000000 INFO @ Thu, 06 Dec 2018 03:20:14: 7000000 INFO @ Thu, 06 Dec 2018 03:20:15: 8000000 INFO @ Thu, 06 Dec 2018 03:20:15: 9000000 INFO @ Thu, 06 Dec 2018 03:20:23: 8000000 INFO @ Thu, 06 Dec 2018 03:20:23: 10000000 INFO @ Thu, 06 Dec 2018 03:20:24: 9000000 INFO @ Thu, 06 Dec 2018 03:20:31: 11000000 INFO @ Thu, 06 Dec 2018 03:20:32: 9000000 INFO @ Thu, 06 Dec 2018 03:20:33: 10000000 INFO @ Thu, 06 Dec 2018 03:20:39: 12000000 INFO @ Thu, 06 Dec 2018 03:20:41: 10000000 INFO @ Thu, 06 Dec 2018 03:20:42: 11000000 INFO @ Thu, 06 Dec 2018 03:20:47: 13000000 INFO @ Thu, 06 Dec 2018 03:20:50: 11000000 INFO @ Thu, 06 Dec 2018 03:20:51: 12000000 INFO @ Thu, 06 Dec 2018 03:20:55: 14000000 INFO @ Thu, 06 Dec 2018 03:20:59: 12000000 INFO @ Thu, 06 Dec 2018 03:20:59: 13000000 INFO @ Thu, 06 Dec 2018 03:21:03: 15000000 INFO @ Thu, 06 Dec 2018 03:21:08: 13000000 INFO @ Thu, 06 Dec 2018 03:21:08: 14000000 INFO @ Thu, 06 Dec 2018 03:21:11: 16000000 INFO @ Thu, 06 Dec 2018 03:21:16: 14000000 INFO @ Thu, 06 Dec 2018 03:21:16: 15000000 INFO @ Thu, 06 Dec 2018 03:21:19: 17000000 INFO @ Thu, 06 Dec 2018 03:21:25: 16000000 INFO @ Thu, 06 Dec 2018 03:21:25: 15000000 INFO @ Thu, 06 Dec 2018 03:21:27: 18000000 INFO @ Thu, 06 Dec 2018 03:21:34: 17000000 INFO @ Thu, 06 Dec 2018 03:21:34: 16000000 INFO @ Thu, 06 Dec 2018 03:21:35: 19000000 INFO @ Thu, 06 Dec 2018 03:21:42: 18000000 INFO @ Thu, 06 Dec 2018 03:21:43: 17000000 INFO @ Thu, 06 Dec 2018 03:21:43: 20000000 INFO @ Thu, 06 Dec 2018 03:21:50: 19000000 INFO @ Thu, 06 Dec 2018 03:21:50: 18000000 INFO @ Thu, 06 Dec 2018 03:21:51: 21000000 INFO @ Thu, 06 Dec 2018 03:21:57: 20000000 INFO @ Thu, 06 Dec 2018 03:21:58: 22000000 INFO @ Thu, 06 Dec 2018 03:21:58: 19000000 INFO @ Thu, 06 Dec 2018 03:22:05: 23000000 INFO @ Thu, 06 Dec 2018 03:22:05: 21000000 INFO @ Thu, 06 Dec 2018 03:22:06: 20000000 INFO @ Thu, 06 Dec 2018 03:22:11: 24000000 INFO @ Thu, 06 Dec 2018 03:22:13: 22000000 INFO @ Thu, 06 Dec 2018 03:22:14: 21000000 INFO @ Thu, 06 Dec 2018 03:22:18: 25000000 INFO @ Thu, 06 Dec 2018 03:22:20: 23000000 INFO @ Thu, 06 Dec 2018 03:22:21: 22000000 INFO @ Thu, 06 Dec 2018 03:22:25: 26000000 INFO @ Thu, 06 Dec 2018 03:22:28: 24000000 INFO @ Thu, 06 Dec 2018 03:22:29: 23000000 INFO @ Thu, 06 Dec 2018 03:22:31: 27000000 INFO @ Thu, 06 Dec 2018 03:22:35: 25000000 INFO @ Thu, 06 Dec 2018 03:22:37: 28000000 INFO @ Thu, 06 Dec 2018 03:22:37: 24000000 INFO @ Thu, 06 Dec 2018 03:22:43: 29000000 INFO @ Thu, 06 Dec 2018 03:22:44: 26000000 INFO @ Thu, 06 Dec 2018 03:22:46: 25000000 INFO @ Thu, 06 Dec 2018 03:22:49: 30000000 INFO @ Thu, 06 Dec 2018 03:22:52: 27000000 INFO @ Thu, 06 Dec 2018 03:22:54: 26000000 INFO @ Thu, 06 Dec 2018 03:22:55: 31000000 INFO @ Thu, 06 Dec 2018 03:23:00: 28000000 INFO @ Thu, 06 Dec 2018 03:23:01: 32000000 INFO @ Thu, 06 Dec 2018 03:23:03: 27000000 INFO @ Thu, 06 Dec 2018 03:23:08: 33000000 INFO @ Thu, 06 Dec 2018 03:23:09: 29000000 INFO @ Thu, 06 Dec 2018 03:23:11: 28000000 INFO @ Thu, 06 Dec 2018 03:23:14: 34000000 INFO @ Thu, 06 Dec 2018 03:23:17: 30000000 INFO @ Thu, 06 Dec 2018 03:23:20: 29000000 INFO @ Thu, 06 Dec 2018 03:23:20: 35000000 INFO @ Thu, 06 Dec 2018 03:23:26: 31000000 INFO @ Thu, 06 Dec 2018 03:23:26: 36000000 INFO @ Thu, 06 Dec 2018 03:23:29: 30000000 INFO @ Thu, 06 Dec 2018 03:23:32: 37000000 INFO @ Thu, 06 Dec 2018 03:23:35: 32000000 INFO @ Thu, 06 Dec 2018 03:23:38: 31000000 INFO @ Thu, 06 Dec 2018 03:23:38: 38000000 INFO @ Thu, 06 Dec 2018 03:23:44: 33000000 INFO @ Thu, 06 Dec 2018 03:23:44: 39000000 INFO @ Thu, 06 Dec 2018 03:23:47: 32000000 INFO @ Thu, 06 Dec 2018 03:23:50: 40000000 INFO @ Thu, 06 Dec 2018 03:23:52: 34000000 INFO @ Thu, 06 Dec 2018 03:23:56: 33000000 INFO @ Thu, 06 Dec 2018 03:23:56: 41000000 INFO @ Thu, 06 Dec 2018 03:24:01: 35000000 INFO @ Thu, 06 Dec 2018 03:24:02: #1 tag size is determined as 50 bps INFO @ Thu, 06 Dec 2018 03:24:02: #1 tag size = 50 INFO @ Thu, 06 Dec 2018 03:24:02: #1 total tags in treatment: 41867537 INFO @ Thu, 06 Dec 2018 03:24:02: #1 user defined the maximum tags... INFO @ Thu, 06 Dec 2018 03:24:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 06 Dec 2018 03:24:03: #1 tags after filtering in treatment: 41867537 INFO @ Thu, 06 Dec 2018 03:24:03: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 06 Dec 2018 03:24:03: #1 finished! INFO @ Thu, 06 Dec 2018 03:24:03: #2 Build Peak Model... INFO @ Thu, 06 Dec 2018 03:24:03: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 06 Dec 2018 03:24:05: 34000000 INFO @ Thu, 06 Dec 2018 03:24:06: #2 number of paired peaks: 25 WARNING @ Thu, 06 Dec 2018 03:24:06: Too few paired peaks (25) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 06 Dec 2018 03:24:06: Process for pairing-model is terminated! cat: SRX4580637.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 3 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4580637.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4580637.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4580637.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Thu, 06 Dec 2018 03:24:10: 36000000 INFO @ Thu, 06 Dec 2018 03:24:14: 35000000 INFO @ Thu, 06 Dec 2018 03:24:19: 37000000 INFO @ Thu, 06 Dec 2018 03:24:23: 36000000 INFO @ Thu, 06 Dec 2018 03:24:28: 38000000 INFO @ Thu, 06 Dec 2018 03:24:31: 37000000 INFO @ Thu, 06 Dec 2018 03:24:37: 39000000 INFO @ Thu, 06 Dec 2018 03:24:40: 38000000 INFO @ Thu, 06 Dec 2018 03:24:46: 40000000 INFO @ Thu, 06 Dec 2018 03:24:50: 39000000 INFO @ Thu, 06 Dec 2018 03:24:55: 41000000 INFO @ Thu, 06 Dec 2018 03:24:59: 40000000 INFO @ Thu, 06 Dec 2018 03:25:04: #1 tag size is determined as 50 bps INFO @ Thu, 06 Dec 2018 03:25:04: #1 tag size = 50 INFO @ Thu, 06 Dec 2018 03:25:04: #1 total tags in treatment: 41867537 INFO @ Thu, 06 Dec 2018 03:25:04: #1 user defined the maximum tags... INFO @ Thu, 06 Dec 2018 03:25:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 06 Dec 2018 03:25:04: #1 tags after filtering in treatment: 41867537 INFO @ Thu, 06 Dec 2018 03:25:04: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 06 Dec 2018 03:25:04: #1 finished! INFO @ Thu, 06 Dec 2018 03:25:04: #2 Build Peak Model... INFO @ Thu, 06 Dec 2018 03:25:04: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 06 Dec 2018 03:25:08: #2 number of paired peaks: 25 WARNING @ Thu, 06 Dec 2018 03:25:08: Too few paired peaks (25) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 06 Dec 2018 03:25:08: Process for pairing-model is terminated! cat: SRX4580637.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4580637.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4580637.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4580637.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Thu, 06 Dec 2018 03:25:08: 41000000 INFO @ Thu, 06 Dec 2018 03:25:16: #1 tag size is determined as 50 bps INFO @ Thu, 06 Dec 2018 03:25:16: #1 tag size = 50 INFO @ Thu, 06 Dec 2018 03:25:16: #1 total tags in treatment: 41867537 INFO @ Thu, 06 Dec 2018 03:25:16: #1 user defined the maximum tags... INFO @ Thu, 06 Dec 2018 03:25:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 06 Dec 2018 03:25:17: #1 tags after filtering in treatment: 41867537 INFO @ Thu, 06 Dec 2018 03:25:17: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 06 Dec 2018 03:25:17: #1 finished! INFO @ Thu, 06 Dec 2018 03:25:17: #2 Build Peak Model... INFO @ Thu, 06 Dec 2018 03:25:17: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 06 Dec 2018 03:25:20: #2 number of paired peaks: 25 WARNING @ Thu, 06 Dec 2018 03:25:20: Too few paired peaks (25) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 06 Dec 2018 03:25:20: Process for pairing-model is terminated! cat: SRX4580637.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 3 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4580637.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4580637.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4580637.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。