Job ID = 3785847 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2019-11-01T05:35:38 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:35:38 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:35:38 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:35:38 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:35:38 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:35:39 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:36:13 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - mbedtls_ssl_handshake returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:36:13 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - ktls_handshake failed while accessing '130.14.250.26' from '172.19.7.18' 2019-11-01T05:36:13 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - Failed to create TLS stream for 'sra-download.ncbi.nlm.nih.gov' (130.14.250.26) from '172.19.7.18' 2019-11-01T05:36:13 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - mbedtls_ssl_handshake returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:36:13 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - ktls_handshake failed while accessing '130.14.250.26' from '172.19.7.18' 2019-11-01T05:36:13 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - Failed to create TLS stream for 'sra-download.ncbi.nlm.nih.gov' (130.14.250.26) from '172.19.7.18' 2019-11-01T05:36:13 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - mbedtls_ssl_handshake returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:36:13 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - ktls_handshake failed while accessing '130.14.250.26' from '172.19.7.18' 2019-11-01T05:36:13 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - Failed to create TLS stream for 'sra-download.ncbi.nlm.nih.gov' (130.14.250.26) from '172.19.7.18' 2019-11-01T05:36:13 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - mbedtls_ssl_handshake returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:36:13 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - ktls_handshake failed while accessing '130.14.250.26' from '172.19.7.18' 2019-11-01T05:36:13 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - Failed to create TLS stream for 'sra-download.ncbi.nlm.nih.gov' (130.14.250.26) from '172.19.7.18' 2019-11-01T05:38:29 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:38:29 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:38:29 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:39:29 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:39:49 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:40:06 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:40:42 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:41:13 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:41:16 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - mbedtls_ssl_handshake returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:41:16 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - ktls_handshake failed while accessing '130.14.250.25' from '172.19.7.18' 2019-11-01T05:41:16 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - Failed to create TLS stream for 'sra-download.ncbi.nlm.nih.gov' (130.14.250.25) from '172.19.7.18' 2019-11-01T05:41:28 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:43:39 fasterq-dump.2.9.6 sys: error unknown while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:44:42 fasterq-dump.2.9.6 sys: error unknown while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:44:44 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:45:15 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:45:32 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:46:07 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:46:11 fasterq-dump.2.9.6 sys: error unknown while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:46:33 fasterq-dump.2.9.6 sys: error unknown while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:46:37 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:47:13 fasterq-dump.2.9.6 sys: error unknown while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:47:53 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:48:30 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:48:43 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:48:43 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:49:57 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:49:57 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:51:10 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - mbedtls_ssl_handshake returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:51:10 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - ktls_handshake failed while accessing '130.14.250.27' from '172.19.7.18' 2019-11-01T05:51:10 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - Failed to create TLS stream for 'sra-download.ncbi.nlm.nih.gov' (130.14.250.27) from '172.19.7.18' 2019-11-01T05:53:15 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:53:15 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:53:55 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:54:51 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:54:52 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:55:22 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:55:22 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:55:43 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:55:58 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:56:22 fasterq-dump.2.9.6 sys: error unknown while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:56:48 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:57:33 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:57:44 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:57:44 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:59:09 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:59:09 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T06:01:14 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T06:04:47 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) spots read : 70,520,001 reads read : 141,040,002 reads written : 70,520,001 reads 0-length : 70,520,001 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:44 70520001 reads; of these: 70520001 (100.00%) were unpaired; of these: 69370896 (98.37%) aligned 0 times 916439 (1.30%) aligned exactly 1 time 232666 (0.33%) aligned >1 times 1.63% overall alignment rate Time searching: 00:07:45 Overall time: 00:07:45 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 854703 / 1149105 = 0.7438 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Fri, 01 Nov 2019 15:23:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4579177/SRX4579177.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4579177/SRX4579177.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4579177/SRX4579177.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4579177/SRX4579177.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 01 Nov 2019 15:23:46: #1 read tag files... INFO @ Fri, 01 Nov 2019 15:23:46: #1 read treatment tags... INFO @ Fri, 01 Nov 2019 15:23:49: #1 tag size is determined as 50 bps INFO @ Fri, 01 Nov 2019 15:23:49: #1 tag size = 50 INFO @ Fri, 01 Nov 2019 15:23:49: #1 total tags in treatment: 294402 INFO @ Fri, 01 Nov 2019 15:23:49: #1 user defined the maximum tags... INFO @ Fri, 01 Nov 2019 15:23:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 01 Nov 2019 15:23:49: #1 tags after filtering in treatment: 294402 INFO @ Fri, 01 Nov 2019 15:23:49: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 01 Nov 2019 15:23:49: #1 finished! INFO @ Fri, 01 Nov 2019 15:23:49: #2 Build Peak Model... INFO @ Fri, 01 Nov 2019 15:23:49: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 01 Nov 2019 15:23:49: #2 number of paired peaks: 4487 INFO @ Fri, 01 Nov 2019 15:23:49: start model_add_line... INFO @ Fri, 01 Nov 2019 15:23:49: start X-correlation... INFO @ Fri, 01 Nov 2019 15:23:49: end of X-cor INFO @ Fri, 01 Nov 2019 15:23:49: #2 finished! INFO @ Fri, 01 Nov 2019 15:23:49: #2 predicted fragment length is 49 bps INFO @ Fri, 01 Nov 2019 15:23:49: #2 alternative fragment length(s) may be 49,146,195,227,257,283 bps INFO @ Fri, 01 Nov 2019 15:23:49: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX4579177/SRX4579177.05_model.r WARNING @ Fri, 01 Nov 2019 15:23:49: #2 Since the d (49) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 01 Nov 2019 15:23:49: #2 You may need to consider one of the other alternative d(s): 49,146,195,227,257,283 WARNING @ Fri, 01 Nov 2019 15:23:49: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 01 Nov 2019 15:23:49: #3 Call peaks... INFO @ Fri, 01 Nov 2019 15:23:49: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 01 Nov 2019 15:23:50: #3 Call peaks for each chromosome... INFO @ Fri, 01 Nov 2019 15:23:50: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX4579177/SRX4579177.05_peaks.xls INFO @ Fri, 01 Nov 2019 15:23:50: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX4579177/SRX4579177.05_peaks.narrowPeak INFO @ Fri, 01 Nov 2019 15:23:50: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX4579177/SRX4579177.05_summits.bed INFO @ Fri, 01 Nov 2019 15:23:50: Done! pass1 - making usageList (12 chroms): 1 millis pass2 - checking and writing primary data (485 records, 4 fields): 4 millis CompletedMACS2peakCalling INFO @ Fri, 01 Nov 2019 15:24:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4579177/SRX4579177.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4579177/SRX4579177.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4579177/SRX4579177.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4579177/SRX4579177.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 01 Nov 2019 15:24:16: #1 read tag files... INFO @ Fri, 01 Nov 2019 15:24:16: #1 read treatment tags... INFO @ Fri, 01 Nov 2019 15:24:18: #1 tag size is determined as 50 bps INFO @ Fri, 01 Nov 2019 15:24:18: #1 tag size = 50 INFO @ Fri, 01 Nov 2019 15:24:18: #1 total tags in treatment: 294402 INFO @ Fri, 01 Nov 2019 15:24:18: #1 user defined the maximum tags... INFO @ Fri, 01 Nov 2019 15:24:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 01 Nov 2019 15:24:18: #1 tags after filtering in treatment: 294402 INFO @ Fri, 01 Nov 2019 15:24:18: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 01 Nov 2019 15:24:18: #1 finished! INFO @ Fri, 01 Nov 2019 15:24:18: #2 Build Peak Model... INFO @ Fri, 01 Nov 2019 15:24:18: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 01 Nov 2019 15:24:19: #2 number of paired peaks: 4487 INFO @ Fri, 01 Nov 2019 15:24:19: start model_add_line... INFO @ Fri, 01 Nov 2019 15:24:19: start X-correlation... INFO @ Fri, 01 Nov 2019 15:24:19: end of X-cor INFO @ Fri, 01 Nov 2019 15:24:19: #2 finished! INFO @ Fri, 01 Nov 2019 15:24:19: #2 predicted fragment length is 49 bps INFO @ Fri, 01 Nov 2019 15:24:19: #2 alternative fragment length(s) may be 49,146,195,227,257,283 bps INFO @ Fri, 01 Nov 2019 15:24:19: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX4579177/SRX4579177.10_model.r WARNING @ Fri, 01 Nov 2019 15:24:19: #2 Since the d (49) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 01 Nov 2019 15:24:19: #2 You may need to consider one of the other alternative d(s): 49,146,195,227,257,283 WARNING @ Fri, 01 Nov 2019 15:24:19: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 01 Nov 2019 15:24:19: #3 Call peaks... INFO @ Fri, 01 Nov 2019 15:24:19: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 01 Nov 2019 15:24:19: #3 Call peaks for each chromosome... INFO @ Fri, 01 Nov 2019 15:24:20: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX4579177/SRX4579177.10_peaks.xls INFO @ Fri, 01 Nov 2019 15:24:20: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX4579177/SRX4579177.10_peaks.narrowPeak INFO @ Fri, 01 Nov 2019 15:24:20: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX4579177/SRX4579177.10_summits.bed INFO @ Fri, 01 Nov 2019 15:24:20: Done! pass1 - making usageList (11 chroms): 1 millis pass2 - checking and writing primary data (349 records, 4 fields): 3 millis CompletedMACS2peakCalling BedGraph に変換中... INFO @ Fri, 01 Nov 2019 15:24:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4579177/SRX4579177.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4579177/SRX4579177.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4579177/SRX4579177.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4579177/SRX4579177.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 01 Nov 2019 15:24:46: #1 read tag files... INFO @ Fri, 01 Nov 2019 15:24:46: #1 read treatment tags... INFO @ Fri, 01 Nov 2019 15:24:49: #1 tag size is determined as 50 bps INFO @ Fri, 01 Nov 2019 15:24:49: #1 tag size = 50 INFO @ Fri, 01 Nov 2019 15:24:49: #1 total tags in treatment: 294402 INFO @ Fri, 01 Nov 2019 15:24:49: #1 user defined the maximum tags... INFO @ Fri, 01 Nov 2019 15:24:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 01 Nov 2019 15:24:49: #1 tags after filtering in treatment: 294402 INFO @ Fri, 01 Nov 2019 15:24:49: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 01 Nov 2019 15:24:49: #1 finished! INFO @ Fri, 01 Nov 2019 15:24:49: #2 Build Peak Model... INFO @ Fri, 01 Nov 2019 15:24:49: #2 looking for paired plus/minus strand peaks... BedGraph に変換しました。 INFO @ Fri, 01 Nov 2019 15:24:49: #2 number of paired peaks: 4487 INFO @ Fri, 01 Nov 2019 15:24:49: start model_add_line... INFO @ Fri, 01 Nov 2019 15:24:49: start X-correlation... BigWig に変換中... INFO @ Fri, 01 Nov 2019 15:24:49: end of X-cor INFO @ Fri, 01 Nov 2019 15:24:49: #2 finished! INFO @ Fri, 01 Nov 2019 15:24:49: #2 predicted fragment length is 49 bps INFO @ Fri, 01 Nov 2019 15:24:49: #2 alternative fragment length(s) may be 49,146,195,227,257,283 bps INFO @ Fri, 01 Nov 2019 15:24:49: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX4579177/SRX4579177.20_model.r WARNING @ Fri, 01 Nov 2019 15:24:49: #2 Since the d (49) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 01 Nov 2019 15:24:49: #2 You may need to consider one of the other alternative d(s): 49,146,195,227,257,283 WARNING @ Fri, 01 Nov 2019 15:24:49: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 01 Nov 2019 15:24:49: #3 Call peaks... INFO @ Fri, 01 Nov 2019 15:24:49: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Fri, 01 Nov 2019 15:24:50: #3 Call peaks for each chromosome... INFO @ Fri, 01 Nov 2019 15:24:50: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX4579177/SRX4579177.20_peaks.xls INFO @ Fri, 01 Nov 2019 15:24:50: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX4579177/SRX4579177.20_peaks.narrowPeak INFO @ Fri, 01 Nov 2019 15:24:50: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX4579177/SRX4579177.20_summits.bed INFO @ Fri, 01 Nov 2019 15:24:50: Done! pass1 - making usageList (9 chroms): 1 millis pass2 - checking and writing primary data (183 records, 4 fields): 2 millis CompletedMACS2peakCalling