Job ID = 3785825 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2019-11-01T05:33:01 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:33:01 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:33:01 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:33:01 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:33:01 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:33:01 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:34:05 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:34:05 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:34:05 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:34:05 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:34:05 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:35:33 fasterq-dump.2.9.6 sys: error unknown while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:35:38 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:35:38 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:35:39 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:36:13 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - mbedtls_ssl_handshake returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:36:13 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - ktls_handshake failed while accessing '130.14.250.26' from '172.19.7.59' 2019-11-01T05:36:13 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - Failed to create TLS stream for 'sra-download.ncbi.nlm.nih.gov' (130.14.250.26) from '172.19.7.59' 2019-11-01T05:36:28 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:37:12 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:37:13 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:39:49 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:39:49 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:39:49 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:40:11 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:40:42 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:41:13 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:41:34 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:41:34 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:43:18 fasterq-dump.2.9.6 sys: error unknown while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:43:32 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:43:39 fasterq-dump.2.9.6 sys: error unknown while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:44:42 fasterq-dump.2.9.6 sys: error unknown while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:44:42 fasterq-dump.2.9.6 sys: error unknown while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:44:44 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:46:27 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:46:37 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:46:45 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:47:13 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:47:13 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:47:30 fasterq-dump.2.9.6 sys: error unknown while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:47:58 fasterq-dump.2.9.6 sys: error unknown while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:48:30 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:50:01 fasterq-dump.2.9.6 sys: error unknown while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:50:01 fasterq-dump.2.9.6 sys: error unknown while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:50:19 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:50:28 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:50:45 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:50:45 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:51:07 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:51:32 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:53:06 fasterq-dump.2.9.6 sys: error unknown while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:53:55 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:54:51 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:55:13 fasterq-dump.2.9.6 sys: timeout exhausted while creating file within network system module - Failed to Make Connection in KClientHttpOpen to 'sra-download.ncbi.nlm.nih.gov:443' 2019-11-01T05:55:22 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:56:08 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:56:48 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:57:10 fasterq-dump.2.9.6 sys: error unknown while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:57:44 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:57:58 fasterq-dump.2.9.6 sys: error unknown while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T06:01:27 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) spots read : 81,664,994 reads read : 81,664,994 reads written : 81,664,994 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:14:07 81664994 reads; of these: 81664994 (100.00%) were unpaired; of these: 70873349 (86.79%) aligned 0 times 5205384 (6.37%) aligned exactly 1 time 5586261 (6.84%) aligned >1 times 13.21% overall alignment rate Time searching: 00:14:07 Overall time: 00:14:07 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 2302239 / 10791645 = 0.2133 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Fri, 01 Nov 2019 15:37:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4579159/SRX4579159.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4579159/SRX4579159.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4579159/SRX4579159.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4579159/SRX4579159.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 01 Nov 2019 15:37:22: #1 read tag files... INFO @ Fri, 01 Nov 2019 15:37:22: #1 read treatment tags... INFO @ Fri, 01 Nov 2019 15:37:31: 1000000 INFO @ Fri, 01 Nov 2019 15:37:39: 2000000 INFO @ Fri, 01 Nov 2019 15:37:46: 3000000 INFO @ Fri, 01 Nov 2019 15:37:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4579159/SRX4579159.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4579159/SRX4579159.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4579159/SRX4579159.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4579159/SRX4579159.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 01 Nov 2019 15:37:52: #1 read tag files... INFO @ Fri, 01 Nov 2019 15:37:52: #1 read treatment tags... INFO @ Fri, 01 Nov 2019 15:37:54: 4000000 INFO @ Fri, 01 Nov 2019 15:38:01: 1000000 INFO @ Fri, 01 Nov 2019 15:38:03: 5000000 INFO @ Fri, 01 Nov 2019 15:38:10: 2000000 INFO @ Fri, 01 Nov 2019 15:38:11: 6000000 INFO @ Fri, 01 Nov 2019 15:38:18: 3000000 BedGraph に変換中... INFO @ Fri, 01 Nov 2019 15:38:20: 7000000 INFO @ Fri, 01 Nov 2019 15:38:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4579159/SRX4579159.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4579159/SRX4579159.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4579159/SRX4579159.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4579159/SRX4579159.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 01 Nov 2019 15:38:22: #1 read tag files... INFO @ Fri, 01 Nov 2019 15:38:22: #1 read treatment tags... INFO @ Fri, 01 Nov 2019 15:38:27: 4000000 INFO @ Fri, 01 Nov 2019 15:38:29: 8000000 INFO @ Fri, 01 Nov 2019 15:38:31: 1000000 INFO @ Fri, 01 Nov 2019 15:38:33: #1 tag size is determined as 50 bps INFO @ Fri, 01 Nov 2019 15:38:33: #1 tag size = 50 INFO @ Fri, 01 Nov 2019 15:38:33: #1 total tags in treatment: 8489406 INFO @ Fri, 01 Nov 2019 15:38:33: #1 user defined the maximum tags... INFO @ Fri, 01 Nov 2019 15:38:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 01 Nov 2019 15:38:33: #1 tags after filtering in treatment: 8489406 INFO @ Fri, 01 Nov 2019 15:38:33: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 01 Nov 2019 15:38:33: #1 finished! INFO @ Fri, 01 Nov 2019 15:38:33: #2 Build Peak Model... INFO @ Fri, 01 Nov 2019 15:38:33: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 01 Nov 2019 15:38:34: #2 number of paired peaks: 1163 INFO @ Fri, 01 Nov 2019 15:38:34: start model_add_line... INFO @ Fri, 01 Nov 2019 15:38:34: start X-correlation... INFO @ Fri, 01 Nov 2019 15:38:34: end of X-cor INFO @ Fri, 01 Nov 2019 15:38:34: #2 finished! INFO @ Fri, 01 Nov 2019 15:38:34: #2 predicted fragment length is 56 bps INFO @ Fri, 01 Nov 2019 15:38:34: #2 alternative fragment length(s) may be 56 bps INFO @ Fri, 01 Nov 2019 15:38:34: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX4579159/SRX4579159.05_model.r WARNING @ Fri, 01 Nov 2019 15:38:34: #2 Since the d (56) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 01 Nov 2019 15:38:34: #2 You may need to consider one of the other alternative d(s): 56 WARNING @ Fri, 01 Nov 2019 15:38:34: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 01 Nov 2019 15:38:34: #3 Call peaks... INFO @ Fri, 01 Nov 2019 15:38:34: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 01 Nov 2019 15:38:35: 5000000 INFO @ Fri, 01 Nov 2019 15:38:39: 2000000 INFO @ Fri, 01 Nov 2019 15:38:43: 6000000 INFO @ Fri, 01 Nov 2019 15:38:48: 3000000 INFO @ Fri, 01 Nov 2019 15:38:51: 7000000 INFO @ Fri, 01 Nov 2019 15:38:56: 4000000 INFO @ Fri, 01 Nov 2019 15:38:57: #3 Call peaks for each chromosome... INFO @ Fri, 01 Nov 2019 15:38:59: 8000000 INFO @ Fri, 01 Nov 2019 15:39:03: #1 tag size is determined as 50 bps INFO @ Fri, 01 Nov 2019 15:39:03: #1 tag size = 50 INFO @ Fri, 01 Nov 2019 15:39:03: #1 total tags in treatment: 8489406 INFO @ Fri, 01 Nov 2019 15:39:03: #1 user defined the maximum tags... INFO @ Fri, 01 Nov 2019 15:39:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 01 Nov 2019 15:39:03: #1 tags after filtering in treatment: 8489406 INFO @ Fri, 01 Nov 2019 15:39:03: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 01 Nov 2019 15:39:03: #1 finished! INFO @ Fri, 01 Nov 2019 15:39:03: #2 Build Peak Model... INFO @ Fri, 01 Nov 2019 15:39:03: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 01 Nov 2019 15:39:04: 5000000 INFO @ Fri, 01 Nov 2019 15:39:04: #2 number of paired peaks: 1163 INFO @ Fri, 01 Nov 2019 15:39:04: start model_add_line... INFO @ Fri, 01 Nov 2019 15:39:04: start X-correlation... INFO @ Fri, 01 Nov 2019 15:39:04: end of X-cor INFO @ Fri, 01 Nov 2019 15:39:04: #2 finished! INFO @ Fri, 01 Nov 2019 15:39:04: #2 predicted fragment length is 56 bps INFO @ Fri, 01 Nov 2019 15:39:04: #2 alternative fragment length(s) may be 56 bps INFO @ Fri, 01 Nov 2019 15:39:04: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX4579159/SRX4579159.10_model.r WARNING @ Fri, 01 Nov 2019 15:39:04: #2 Since the d (56) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 01 Nov 2019 15:39:04: #2 You may need to consider one of the other alternative d(s): 56 WARNING @ Fri, 01 Nov 2019 15:39:04: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 01 Nov 2019 15:39:04: #3 Call peaks... INFO @ Fri, 01 Nov 2019 15:39:04: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 01 Nov 2019 15:39:09: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX4579159/SRX4579159.05_peaks.xls INFO @ Fri, 01 Nov 2019 15:39:09: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX4579159/SRX4579159.05_peaks.narrowPeak INFO @ Fri, 01 Nov 2019 15:39:09: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX4579159/SRX4579159.05_summits.bed INFO @ Fri, 01 Nov 2019 15:39:09: Done! pass1 - making usageList (14 chroms): 2 millis pass2 - checking and writing primary data (4052 records, 4 fields): 15 millis CompletedMACS2peakCalling INFO @ Fri, 01 Nov 2019 15:39:12: 6000000 INFO @ Fri, 01 Nov 2019 15:39:20: 7000000 INFO @ Fri, 01 Nov 2019 15:39:28: #3 Call peaks for each chromosome... INFO @ Fri, 01 Nov 2019 15:39:28: 8000000 INFO @ Fri, 01 Nov 2019 15:39:32: #1 tag size is determined as 50 bps INFO @ Fri, 01 Nov 2019 15:39:32: #1 tag size = 50 INFO @ Fri, 01 Nov 2019 15:39:32: #1 total tags in treatment: 8489406 INFO @ Fri, 01 Nov 2019 15:39:32: #1 user defined the maximum tags... INFO @ Fri, 01 Nov 2019 15:39:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 01 Nov 2019 15:39:32: #1 tags after filtering in treatment: 8489406 INFO @ Fri, 01 Nov 2019 15:39:32: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 01 Nov 2019 15:39:32: #1 finished! INFO @ Fri, 01 Nov 2019 15:39:32: #2 Build Peak Model... INFO @ Fri, 01 Nov 2019 15:39:32: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 01 Nov 2019 15:39:33: #2 number of paired peaks: 1163 INFO @ Fri, 01 Nov 2019 15:39:33: start model_add_line... INFO @ Fri, 01 Nov 2019 15:39:33: start X-correlation... INFO @ Fri, 01 Nov 2019 15:39:33: end of X-cor INFO @ Fri, 01 Nov 2019 15:39:33: #2 finished! INFO @ Fri, 01 Nov 2019 15:39:33: #2 predicted fragment length is 56 bps INFO @ Fri, 01 Nov 2019 15:39:33: #2 alternative fragment length(s) may be 56 bps INFO @ Fri, 01 Nov 2019 15:39:33: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX4579159/SRX4579159.20_model.r WARNING @ Fri, 01 Nov 2019 15:39:33: #2 Since the d (56) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 01 Nov 2019 15:39:33: #2 You may need to consider one of the other alternative d(s): 56 WARNING @ Fri, 01 Nov 2019 15:39:33: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 01 Nov 2019 15:39:33: #3 Call peaks... INFO @ Fri, 01 Nov 2019 15:39:33: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 01 Nov 2019 15:39:39: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX4579159/SRX4579159.10_peaks.xls INFO @ Fri, 01 Nov 2019 15:39:39: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX4579159/SRX4579159.10_peaks.narrowPeak INFO @ Fri, 01 Nov 2019 15:39:39: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX4579159/SRX4579159.10_summits.bed INFO @ Fri, 01 Nov 2019 15:39:39: Done! pass1 - making usageList (12 chroms): 2 millis pass2 - checking and writing primary data (2246 records, 4 fields): 5 millis CompletedMACS2peakCalling INFO @ Fri, 01 Nov 2019 15:39:57: #3 Call peaks for each chromosome... INFO @ Fri, 01 Nov 2019 15:40:08: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX4579159/SRX4579159.20_peaks.xls INFO @ Fri, 01 Nov 2019 15:40:08: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX4579159/SRX4579159.20_peaks.narrowPeak INFO @ Fri, 01 Nov 2019 15:40:08: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX4579159/SRX4579159.20_summits.bed INFO @ Fri, 01 Nov 2019 15:40:08: Done! pass1 - making usageList (12 chroms): 2 millis pass2 - checking and writing primary data (1241 records, 4 fields): 5 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。