Job ID = 12265230 SRX = SRX4518001 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 44461472 spots for SRR7655605/SRR7655605.sra Written 44461472 spots for SRR7655605/SRR7655605.sra fastq に変換しました。 bowtie でマッピング中... Your job 12265567 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:40:19 44461472 reads; of these: 44461472 (100.00%) were paired; of these: 5834288 (13.12%) aligned concordantly 0 times 36700447 (82.54%) aligned concordantly exactly 1 time 1926737 (4.33%) aligned concordantly >1 times ---- 5834288 pairs aligned concordantly 0 times; of these: 2882961 (49.41%) aligned discordantly 1 time ---- 2951327 pairs aligned 0 times concordantly or discordantly; of these: 5902654 mates make up the pairs; of these: 4659378 (78.94%) aligned 0 times 892121 (15.11%) aligned exactly 1 time 351155 (5.95%) aligned >1 times 94.76% overall alignment rate Time searching: 00:40:20 Overall time: 00:40:20 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 36 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 4069850 / 40940237 = 0.0994 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:33:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4518001/SRX4518001.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4518001/SRX4518001.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4518001/SRX4518001.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4518001/SRX4518001.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:33:43: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:33:43: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:33:50: 1000000 INFO @ Sat, 03 Apr 2021 07:33:56: 2000000 INFO @ Sat, 03 Apr 2021 07:34:03: 3000000 INFO @ Sat, 03 Apr 2021 07:34:09: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:34:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4518001/SRX4518001.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4518001/SRX4518001.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4518001/SRX4518001.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4518001/SRX4518001.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:34:14: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:34:14: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:34:16: 5000000 INFO @ Sat, 03 Apr 2021 07:34:21: 1000000 INFO @ Sat, 03 Apr 2021 07:34:23: 6000000 INFO @ Sat, 03 Apr 2021 07:34:28: 2000000 INFO @ Sat, 03 Apr 2021 07:34:30: 7000000 INFO @ Sat, 03 Apr 2021 07:34:35: 3000000 INFO @ Sat, 03 Apr 2021 07:34:37: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:34:41: 4000000 INFO @ Sat, 03 Apr 2021 07:34:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4518001/SRX4518001.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4518001/SRX4518001.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4518001/SRX4518001.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4518001/SRX4518001.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:34:43: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:34:43: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:34:43: 9000000 INFO @ Sat, 03 Apr 2021 07:34:49: 5000000 INFO @ Sat, 03 Apr 2021 07:34:49: 1000000 INFO @ Sat, 03 Apr 2021 07:34:51: 10000000 INFO @ Sat, 03 Apr 2021 07:34:54: 2000000 INFO @ Sat, 03 Apr 2021 07:34:56: 6000000 INFO @ Sat, 03 Apr 2021 07:34:58: 11000000 INFO @ Sat, 03 Apr 2021 07:34:59: 3000000 INFO @ Sat, 03 Apr 2021 07:35:03: 7000000 INFO @ Sat, 03 Apr 2021 07:35:04: 4000000 INFO @ Sat, 03 Apr 2021 07:35:05: 12000000 INFO @ Sat, 03 Apr 2021 07:35:09: 5000000 INFO @ Sat, 03 Apr 2021 07:35:10: 8000000 INFO @ Sat, 03 Apr 2021 07:35:12: 13000000 INFO @ Sat, 03 Apr 2021 07:35:15: 6000000 INFO @ Sat, 03 Apr 2021 07:35:17: 9000000 INFO @ Sat, 03 Apr 2021 07:35:19: 14000000 INFO @ Sat, 03 Apr 2021 07:35:20: 7000000 INFO @ Sat, 03 Apr 2021 07:35:24: 10000000 INFO @ Sat, 03 Apr 2021 07:35:25: 8000000 INFO @ Sat, 03 Apr 2021 07:35:26: 15000000 INFO @ Sat, 03 Apr 2021 07:35:30: 9000000 INFO @ Sat, 03 Apr 2021 07:35:31: 11000000 INFO @ Sat, 03 Apr 2021 07:35:33: 16000000 INFO @ Sat, 03 Apr 2021 07:35:36: 10000000 INFO @ Sat, 03 Apr 2021 07:35:38: 12000000 INFO @ Sat, 03 Apr 2021 07:35:40: 17000000 INFO @ Sat, 03 Apr 2021 07:35:41: 11000000 INFO @ Sat, 03 Apr 2021 07:35:45: 13000000 INFO @ Sat, 03 Apr 2021 07:35:46: 12000000 INFO @ Sat, 03 Apr 2021 07:35:47: 18000000 INFO @ Sat, 03 Apr 2021 07:35:52: 14000000 INFO @ Sat, 03 Apr 2021 07:35:52: 13000000 INFO @ Sat, 03 Apr 2021 07:35:54: 19000000 INFO @ Sat, 03 Apr 2021 07:35:58: 14000000 INFO @ Sat, 03 Apr 2021 07:35:58: 15000000 INFO @ Sat, 03 Apr 2021 07:36:00: 20000000 INFO @ Sat, 03 Apr 2021 07:36:03: 15000000 INFO @ Sat, 03 Apr 2021 07:36:05: 16000000 INFO @ Sat, 03 Apr 2021 07:36:07: 21000000 INFO @ Sat, 03 Apr 2021 07:36:08: 16000000 INFO @ Sat, 03 Apr 2021 07:36:12: 17000000 INFO @ Sat, 03 Apr 2021 07:36:13: 17000000 INFO @ Sat, 03 Apr 2021 07:36:13: 22000000 INFO @ Sat, 03 Apr 2021 07:36:18: 18000000 INFO @ Sat, 03 Apr 2021 07:36:19: 18000000 INFO @ Sat, 03 Apr 2021 07:36:20: 23000000 INFO @ Sat, 03 Apr 2021 07:36:25: 19000000 INFO @ Sat, 03 Apr 2021 07:36:25: 19000000 INFO @ Sat, 03 Apr 2021 07:36:26: 24000000 INFO @ Sat, 03 Apr 2021 07:36:30: 20000000 INFO @ Sat, 03 Apr 2021 07:36:31: 20000000 INFO @ Sat, 03 Apr 2021 07:36:32: 25000000 INFO @ Sat, 03 Apr 2021 07:36:35: 21000000 INFO @ Sat, 03 Apr 2021 07:36:37: 21000000 INFO @ Sat, 03 Apr 2021 07:36:39: 26000000 INFO @ Sat, 03 Apr 2021 07:36:40: 22000000 INFO @ Sat, 03 Apr 2021 07:36:43: 22000000 INFO @ Sat, 03 Apr 2021 07:36:45: 27000000 INFO @ Sat, 03 Apr 2021 07:36:45: 23000000 INFO @ Sat, 03 Apr 2021 07:36:50: 23000000 INFO @ Sat, 03 Apr 2021 07:36:51: 24000000 INFO @ Sat, 03 Apr 2021 07:36:51: 28000000 INFO @ Sat, 03 Apr 2021 07:36:56: 24000000 INFO @ Sat, 03 Apr 2021 07:36:56: 25000000 INFO @ Sat, 03 Apr 2021 07:36:58: 29000000 INFO @ Sat, 03 Apr 2021 07:37:01: 26000000 INFO @ Sat, 03 Apr 2021 07:37:03: 25000000 INFO @ Sat, 03 Apr 2021 07:37:04: 30000000 INFO @ Sat, 03 Apr 2021 07:37:07: 27000000 INFO @ Sat, 03 Apr 2021 07:37:09: 26000000 INFO @ Sat, 03 Apr 2021 07:37:11: 31000000 INFO @ Sat, 03 Apr 2021 07:37:12: 28000000 INFO @ Sat, 03 Apr 2021 07:37:15: 27000000 INFO @ Sat, 03 Apr 2021 07:37:17: 32000000 INFO @ Sat, 03 Apr 2021 07:37:17: 29000000 INFO @ Sat, 03 Apr 2021 07:37:22: 28000000 INFO @ Sat, 03 Apr 2021 07:37:23: 30000000 INFO @ Sat, 03 Apr 2021 07:37:23: 33000000 INFO @ Sat, 03 Apr 2021 07:37:28: 31000000 INFO @ Sat, 03 Apr 2021 07:37:28: 29000000 INFO @ Sat, 03 Apr 2021 07:37:30: 34000000 INFO @ Sat, 03 Apr 2021 07:37:33: 32000000 INFO @ Sat, 03 Apr 2021 07:37:34: 30000000 INFO @ Sat, 03 Apr 2021 07:37:36: 35000000 INFO @ Sat, 03 Apr 2021 07:37:38: 33000000 INFO @ Sat, 03 Apr 2021 07:37:41: 31000000 INFO @ Sat, 03 Apr 2021 07:37:43: 36000000 INFO @ Sat, 03 Apr 2021 07:37:43: 34000000 INFO @ Sat, 03 Apr 2021 07:37:47: 32000000 INFO @ Sat, 03 Apr 2021 07:37:49: 35000000 INFO @ Sat, 03 Apr 2021 07:37:49: 37000000 INFO @ Sat, 03 Apr 2021 07:37:54: 33000000 INFO @ Sat, 03 Apr 2021 07:37:55: 36000000 INFO @ Sat, 03 Apr 2021 07:37:55: 38000000 INFO @ Sat, 03 Apr 2021 07:38:00: 37000000 INFO @ Sat, 03 Apr 2021 07:38:00: 34000000 INFO @ Sat, 03 Apr 2021 07:38:02: 39000000 INFO @ Sat, 03 Apr 2021 07:38:05: 38000000 INFO @ Sat, 03 Apr 2021 07:38:06: 35000000 INFO @ Sat, 03 Apr 2021 07:38:08: 40000000 INFO @ Sat, 03 Apr 2021 07:38:10: 39000000 INFO @ Sat, 03 Apr 2021 07:38:13: 36000000 INFO @ Sat, 03 Apr 2021 07:38:14: 41000000 INFO @ Sat, 03 Apr 2021 07:38:15: 40000000 INFO @ Sat, 03 Apr 2021 07:38:19: 37000000 INFO @ Sat, 03 Apr 2021 07:38:20: 42000000 INFO @ Sat, 03 Apr 2021 07:38:20: 41000000 INFO @ Sat, 03 Apr 2021 07:38:25: 38000000 INFO @ Sat, 03 Apr 2021 07:38:25: 42000000 INFO @ Sat, 03 Apr 2021 07:38:26: 43000000 INFO @ Sat, 03 Apr 2021 07:38:31: 43000000 INFO @ Sat, 03 Apr 2021 07:38:31: 39000000 INFO @ Sat, 03 Apr 2021 07:38:32: 44000000 INFO @ Sat, 03 Apr 2021 07:38:36: 44000000 INFO @ Sat, 03 Apr 2021 07:38:37: 40000000 INFO @ Sat, 03 Apr 2021 07:38:38: 45000000 INFO @ Sat, 03 Apr 2021 07:38:41: 45000000 INFO @ Sat, 03 Apr 2021 07:38:43: 41000000 INFO @ Sat, 03 Apr 2021 07:38:44: 46000000 INFO @ Sat, 03 Apr 2021 07:38:46: 46000000 INFO @ Sat, 03 Apr 2021 07:38:48: 42000000 INFO @ Sat, 03 Apr 2021 07:38:49: 47000000 INFO @ Sat, 03 Apr 2021 07:38:51: 47000000 INFO @ Sat, 03 Apr 2021 07:38:54: 43000000 INFO @ Sat, 03 Apr 2021 07:38:55: 48000000 INFO @ Sat, 03 Apr 2021 07:38:56: 48000000 INFO @ Sat, 03 Apr 2021 07:39:00: 44000000 INFO @ Sat, 03 Apr 2021 07:39:01: 49000000 INFO @ Sat, 03 Apr 2021 07:39:02: 49000000 INFO @ Sat, 03 Apr 2021 07:39:06: 45000000 INFO @ Sat, 03 Apr 2021 07:39:08: 50000000 INFO @ Sat, 03 Apr 2021 07:39:08: 50000000 INFO @ Sat, 03 Apr 2021 07:39:13: 51000000 INFO @ Sat, 03 Apr 2021 07:39:13: 46000000 INFO @ Sat, 03 Apr 2021 07:39:14: 51000000 INFO @ Sat, 03 Apr 2021 07:39:18: 52000000 INFO @ Sat, 03 Apr 2021 07:39:19: 47000000 INFO @ Sat, 03 Apr 2021 07:39:20: 52000000 INFO @ Sat, 03 Apr 2021 07:39:23: 53000000 INFO @ Sat, 03 Apr 2021 07:39:26: 48000000 INFO @ Sat, 03 Apr 2021 07:39:27: 53000000 INFO @ Sat, 03 Apr 2021 07:39:29: 54000000 INFO @ Sat, 03 Apr 2021 07:39:32: 49000000 INFO @ Sat, 03 Apr 2021 07:39:33: 54000000 INFO @ Sat, 03 Apr 2021 07:39:34: 55000000 INFO @ Sat, 03 Apr 2021 07:39:38: 50000000 INFO @ Sat, 03 Apr 2021 07:39:39: 55000000 INFO @ Sat, 03 Apr 2021 07:39:40: 56000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 03 Apr 2021 07:39:44: 51000000 INFO @ Sat, 03 Apr 2021 07:39:45: 57000000 INFO @ Sat, 03 Apr 2021 07:39:45: 56000000 INFO @ Sat, 03 Apr 2021 07:39:50: 52000000 INFO @ Sat, 03 Apr 2021 07:39:50: 58000000 INFO @ Sat, 03 Apr 2021 07:39:51: 57000000 INFO @ Sat, 03 Apr 2021 07:39:55: 59000000 INFO @ Sat, 03 Apr 2021 07:39:56: 53000000 INFO @ Sat, 03 Apr 2021 07:39:57: 58000000 INFO @ Sat, 03 Apr 2021 07:40:01: 60000000 INFO @ Sat, 03 Apr 2021 07:40:02: 54000000 INFO @ Sat, 03 Apr 2021 07:40:02: 59000000 INFO @ Sat, 03 Apr 2021 07:40:06: 61000000 INFO @ Sat, 03 Apr 2021 07:40:08: 55000000 INFO @ Sat, 03 Apr 2021 07:40:08: 60000000 INFO @ Sat, 03 Apr 2021 07:40:11: 62000000 INFO @ Sat, 03 Apr 2021 07:40:14: 56000000 INFO @ Sat, 03 Apr 2021 07:40:14: 61000000 INFO @ Sat, 03 Apr 2021 07:40:16: 63000000 INFO @ Sat, 03 Apr 2021 07:40:20: 57000000 INFO @ Sat, 03 Apr 2021 07:40:21: 62000000 INFO @ Sat, 03 Apr 2021 07:40:22: 64000000 INFO @ Sat, 03 Apr 2021 07:40:26: 58000000 INFO @ Sat, 03 Apr 2021 07:40:27: 63000000 INFO @ Sat, 03 Apr 2021 07:40:27: 65000000 INFO @ Sat, 03 Apr 2021 07:40:32: 59000000 INFO @ Sat, 03 Apr 2021 07:40:33: 66000000 INFO @ Sat, 03 Apr 2021 07:40:33: 64000000 INFO @ Sat, 03 Apr 2021 07:40:38: 60000000 INFO @ Sat, 03 Apr 2021 07:40:38: 67000000 INFO @ Sat, 03 Apr 2021 07:40:39: 65000000 INFO @ Sat, 03 Apr 2021 07:40:43: 68000000 INFO @ Sat, 03 Apr 2021 07:40:43: 61000000 INFO @ Sat, 03 Apr 2021 07:40:45: 66000000 INFO @ Sat, 03 Apr 2021 07:40:49: 69000000 INFO @ Sat, 03 Apr 2021 07:40:50: 62000000 INFO @ Sat, 03 Apr 2021 07:40:51: 67000000 INFO @ Sat, 03 Apr 2021 07:40:54: 70000000 INFO @ Sat, 03 Apr 2021 07:40:56: 63000000 INFO @ Sat, 03 Apr 2021 07:40:57: 68000000 INFO @ Sat, 03 Apr 2021 07:40:59: 71000000 INFO @ Sat, 03 Apr 2021 07:41:02: 64000000 INFO @ Sat, 03 Apr 2021 07:41:03: 69000000 INFO @ Sat, 03 Apr 2021 07:41:04: 72000000 INFO @ Sat, 03 Apr 2021 07:41:08: 65000000 INFO @ Sat, 03 Apr 2021 07:41:08: 70000000 INFO @ Sat, 03 Apr 2021 07:41:10: 73000000 INFO @ Sat, 03 Apr 2021 07:41:14: 66000000 INFO @ Sat, 03 Apr 2021 07:41:14: 71000000 INFO @ Sat, 03 Apr 2021 07:41:15: 74000000 INFO @ Sat, 03 Apr 2021 07:41:20: 67000000 BigWig に変換しました。 INFO @ Sat, 03 Apr 2021 07:41:21: 75000000 INFO @ Sat, 03 Apr 2021 07:41:21: 72000000 INFO @ Sat, 03 Apr 2021 07:41:26: 68000000 INFO @ Sat, 03 Apr 2021 07:41:26: 76000000 INFO @ Sat, 03 Apr 2021 07:41:27: 73000000 INFO @ Sat, 03 Apr 2021 07:41:27: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 07:41:27: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 07:41:27: #1 total tags in treatment: 35029143 INFO @ Sat, 03 Apr 2021 07:41:27: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:41:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 07:41:28: #1 tags after filtering in treatment: 21913404 INFO @ Sat, 03 Apr 2021 07:41:28: #1 Redundant rate of treatment: 0.37 INFO @ Sat, 03 Apr 2021 07:41:28: #1 finished! INFO @ Sat, 03 Apr 2021 07:41:28: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:41:28: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 07:41:30: #2 number of paired peaks: 318 WARNING @ Sat, 03 Apr 2021 07:41:30: Fewer paired peaks (318) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 318 pairs to build model! INFO @ Sat, 03 Apr 2021 07:41:30: start model_add_line... INFO @ Sat, 03 Apr 2021 07:41:30: start X-correlation... INFO @ Sat, 03 Apr 2021 07:41:30: end of X-cor INFO @ Sat, 03 Apr 2021 07:41:30: #2 finished! INFO @ Sat, 03 Apr 2021 07:41:30: #2 predicted fragment length is 15 bps INFO @ Sat, 03 Apr 2021 07:41:30: #2 alternative fragment length(s) may be 4,15,41,577 bps INFO @ Sat, 03 Apr 2021 07:41:30: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX4518001/SRX4518001.20_model.r WARNING @ Sat, 03 Apr 2021 07:41:30: #2 Since the d (15) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 07:41:30: #2 You may need to consider one of the other alternative d(s): 4,15,41,577 WARNING @ Sat, 03 Apr 2021 07:41:30: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 07:41:30: #3 Call peaks... INFO @ Sat, 03 Apr 2021 07:41:30: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 07:41:32: 69000000 INFO @ Sat, 03 Apr 2021 07:41:33: 74000000 INFO @ Sat, 03 Apr 2021 07:41:38: 70000000 INFO @ Sat, 03 Apr 2021 07:41:39: 75000000 INFO @ Sat, 03 Apr 2021 07:41:45: 71000000 INFO @ Sat, 03 Apr 2021 07:41:46: 76000000 INFO @ Sat, 03 Apr 2021 07:41:47: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 07:41:47: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 07:41:47: #1 total tags in treatment: 35029143 INFO @ Sat, 03 Apr 2021 07:41:47: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:41:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 07:41:48: #1 tags after filtering in treatment: 21913404 INFO @ Sat, 03 Apr 2021 07:41:48: #1 Redundant rate of treatment: 0.37 INFO @ Sat, 03 Apr 2021 07:41:48: #1 finished! INFO @ Sat, 03 Apr 2021 07:41:48: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:41:48: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 07:41:49: #2 number of paired peaks: 318 WARNING @ Sat, 03 Apr 2021 07:41:49: Fewer paired peaks (318) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 318 pairs to build model! INFO @ Sat, 03 Apr 2021 07:41:49: start model_add_line... INFO @ Sat, 03 Apr 2021 07:41:49: start X-correlation... INFO @ Sat, 03 Apr 2021 07:41:49: end of X-cor INFO @ Sat, 03 Apr 2021 07:41:49: #2 finished! INFO @ Sat, 03 Apr 2021 07:41:49: #2 predicted fragment length is 15 bps INFO @ Sat, 03 Apr 2021 07:41:49: #2 alternative fragment length(s) may be 4,15,41,577 bps INFO @ Sat, 03 Apr 2021 07:41:49: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX4518001/SRX4518001.05_model.r WARNING @ Sat, 03 Apr 2021 07:41:49: #2 Since the d (15) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 07:41:49: #2 You may need to consider one of the other alternative d(s): 4,15,41,577 WARNING @ Sat, 03 Apr 2021 07:41:49: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 07:41:49: #3 Call peaks... INFO @ Sat, 03 Apr 2021 07:41:49: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 07:41:50: 72000000 INFO @ Sat, 03 Apr 2021 07:41:56: 73000000 INFO @ Sat, 03 Apr 2021 07:42:01: 74000000 INFO @ Sat, 03 Apr 2021 07:42:04: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 07:42:07: 75000000 INFO @ Sat, 03 Apr 2021 07:42:12: 76000000 INFO @ Sat, 03 Apr 2021 07:42:13: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 07:42:13: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 07:42:13: #1 total tags in treatment: 35029143 INFO @ Sat, 03 Apr 2021 07:42:13: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:42:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 07:42:14: #1 tags after filtering in treatment: 21913404 INFO @ Sat, 03 Apr 2021 07:42:14: #1 Redundant rate of treatment: 0.37 INFO @ Sat, 03 Apr 2021 07:42:14: #1 finished! INFO @ Sat, 03 Apr 2021 07:42:14: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:42:14: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 07:42:16: #2 number of paired peaks: 318 WARNING @ Sat, 03 Apr 2021 07:42:16: Fewer paired peaks (318) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 318 pairs to build model! INFO @ Sat, 03 Apr 2021 07:42:16: start model_add_line... INFO @ Sat, 03 Apr 2021 07:42:16: start X-correlation... INFO @ Sat, 03 Apr 2021 07:42:16: end of X-cor INFO @ Sat, 03 Apr 2021 07:42:16: #2 finished! INFO @ Sat, 03 Apr 2021 07:42:16: #2 predicted fragment length is 15 bps INFO @ Sat, 03 Apr 2021 07:42:16: #2 alternative fragment length(s) may be 4,15,41,577 bps INFO @ Sat, 03 Apr 2021 07:42:16: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX4518001/SRX4518001.10_model.r WARNING @ Sat, 03 Apr 2021 07:42:16: #2 Since the d (15) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 07:42:16: #2 You may need to consider one of the other alternative d(s): 4,15,41,577 WARNING @ Sat, 03 Apr 2021 07:42:16: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 07:42:16: #3 Call peaks... INFO @ Sat, 03 Apr 2021 07:42:16: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 07:42:21: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX4518001/SRX4518001.20_peaks.xls INFO @ Sat, 03 Apr 2021 07:42:21: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX4518001/SRX4518001.20_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 07:42:21: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX4518001/SRX4518001.20_summits.bed INFO @ Sat, 03 Apr 2021 07:42:21: Done! pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 07:42:25: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 07:42:43: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX4518001/SRX4518001.05_peaks.xls INFO @ Sat, 03 Apr 2021 07:42:43: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX4518001/SRX4518001.05_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 07:42:43: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX4518001/SRX4518001.05_summits.bed INFO @ Sat, 03 Apr 2021 07:42:43: Done! pass1 - making usageList (12 chroms): 1 millis pass2 - checking and writing primary data (2084 records, 4 fields): 4 millis CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 07:42:51: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 07:43:09: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX4518001/SRX4518001.10_peaks.xls INFO @ Sat, 03 Apr 2021 07:43:09: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX4518001/SRX4518001.10_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 07:43:09: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX4518001/SRX4518001.10_summits.bed INFO @ Sat, 03 Apr 2021 07:43:09: Done! pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) CompletedMACS2peakCalling