Job ID = 12265229 SRX = SRX4518000 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 43374483 spots for SRR7655604/SRR7655604.sra Written 43374483 spots for SRR7655604/SRR7655604.sra fastq に変換しました。 bowtie でマッピング中... Your job 12265546 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:35:23 43374483 reads; of these: 43374483 (100.00%) were paired; of these: 7540542 (17.38%) aligned concordantly 0 times 34204329 (78.86%) aligned concordantly exactly 1 time 1629612 (3.76%) aligned concordantly >1 times ---- 7540542 pairs aligned concordantly 0 times; of these: 3771526 (50.02%) aligned discordantly 1 time ---- 3769016 pairs aligned 0 times concordantly or discordantly; of these: 7538032 mates make up the pairs; of these: 6006268 (79.68%) aligned 0 times 1098313 (14.57%) aligned exactly 1 time 433451 (5.75%) aligned >1 times 93.08% overall alignment rate Time searching: 00:35:23 Overall time: 00:35:23 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 36 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 4081785 / 38927899 = 0.1049 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:27:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4518000/SRX4518000.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4518000/SRX4518000.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4518000/SRX4518000.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4518000/SRX4518000.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:27:59: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:27:59: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:28:04: 1000000 INFO @ Sat, 03 Apr 2021 07:28:09: 2000000 INFO @ Sat, 03 Apr 2021 07:28:14: 3000000 INFO @ Sat, 03 Apr 2021 07:28:19: 4000000 INFO @ Sat, 03 Apr 2021 07:28:23: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:28:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4518000/SRX4518000.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4518000/SRX4518000.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4518000/SRX4518000.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4518000/SRX4518000.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:28:28: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:28:28: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:28:28: 6000000 INFO @ Sat, 03 Apr 2021 07:28:33: 1000000 INFO @ Sat, 03 Apr 2021 07:28:33: 7000000 INFO @ Sat, 03 Apr 2021 07:28:38: 2000000 INFO @ Sat, 03 Apr 2021 07:28:39: 8000000 INFO @ Sat, 03 Apr 2021 07:28:43: 3000000 INFO @ Sat, 03 Apr 2021 07:28:44: 9000000 INFO @ Sat, 03 Apr 2021 07:28:48: 4000000 INFO @ Sat, 03 Apr 2021 07:28:49: 10000000 INFO @ Sat, 03 Apr 2021 07:28:53: 5000000 INFO @ Sat, 03 Apr 2021 07:28:54: 11000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:28:58: 6000000 INFO @ Sat, 03 Apr 2021 07:28:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4518000/SRX4518000.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4518000/SRX4518000.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4518000/SRX4518000.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4518000/SRX4518000.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:28:58: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:28:58: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:28:59: 12000000 INFO @ Sat, 03 Apr 2021 07:29:03: 7000000 INFO @ Sat, 03 Apr 2021 07:29:04: 1000000 INFO @ Sat, 03 Apr 2021 07:29:04: 13000000 INFO @ Sat, 03 Apr 2021 07:29:08: 8000000 INFO @ Sat, 03 Apr 2021 07:29:09: 2000000 INFO @ Sat, 03 Apr 2021 07:29:10: 14000000 INFO @ Sat, 03 Apr 2021 07:29:13: 9000000 INFO @ Sat, 03 Apr 2021 07:29:15: 3000000 INFO @ Sat, 03 Apr 2021 07:29:16: 15000000 INFO @ Sat, 03 Apr 2021 07:29:18: 10000000 INFO @ Sat, 03 Apr 2021 07:29:21: 4000000 INFO @ Sat, 03 Apr 2021 07:29:21: 16000000 INFO @ Sat, 03 Apr 2021 07:29:23: 11000000 INFO @ Sat, 03 Apr 2021 07:29:26: 5000000 INFO @ Sat, 03 Apr 2021 07:29:27: 17000000 INFO @ Sat, 03 Apr 2021 07:29:28: 12000000 INFO @ Sat, 03 Apr 2021 07:29:32: 6000000 INFO @ Sat, 03 Apr 2021 07:29:32: 18000000 INFO @ Sat, 03 Apr 2021 07:29:33: 13000000 INFO @ Sat, 03 Apr 2021 07:29:38: 14000000 INFO @ Sat, 03 Apr 2021 07:29:38: 7000000 INFO @ Sat, 03 Apr 2021 07:29:38: 19000000 INFO @ Sat, 03 Apr 2021 07:29:43: 15000000 INFO @ Sat, 03 Apr 2021 07:29:44: 8000000 INFO @ Sat, 03 Apr 2021 07:29:44: 20000000 INFO @ Sat, 03 Apr 2021 07:29:48: 16000000 INFO @ Sat, 03 Apr 2021 07:29:49: 9000000 INFO @ Sat, 03 Apr 2021 07:29:50: 21000000 INFO @ Sat, 03 Apr 2021 07:29:53: 17000000 INFO @ Sat, 03 Apr 2021 07:29:55: 10000000 INFO @ Sat, 03 Apr 2021 07:29:55: 22000000 INFO @ Sat, 03 Apr 2021 07:29:58: 18000000 INFO @ Sat, 03 Apr 2021 07:30:00: 11000000 INFO @ Sat, 03 Apr 2021 07:30:01: 23000000 INFO @ Sat, 03 Apr 2021 07:30:03: 19000000 INFO @ Sat, 03 Apr 2021 07:30:06: 12000000 INFO @ Sat, 03 Apr 2021 07:30:06: 24000000 INFO @ Sat, 03 Apr 2021 07:30:08: 20000000 INFO @ Sat, 03 Apr 2021 07:30:11: 13000000 INFO @ Sat, 03 Apr 2021 07:30:12: 25000000 INFO @ Sat, 03 Apr 2021 07:30:13: 21000000 INFO @ Sat, 03 Apr 2021 07:30:17: 14000000 INFO @ Sat, 03 Apr 2021 07:30:17: 26000000 INFO @ Sat, 03 Apr 2021 07:30:17: 22000000 INFO @ Sat, 03 Apr 2021 07:30:22: 23000000 INFO @ Sat, 03 Apr 2021 07:30:22: 15000000 INFO @ Sat, 03 Apr 2021 07:30:23: 27000000 INFO @ Sat, 03 Apr 2021 07:30:27: 24000000 INFO @ Sat, 03 Apr 2021 07:30:28: 16000000 INFO @ Sat, 03 Apr 2021 07:30:28: 28000000 INFO @ Sat, 03 Apr 2021 07:30:32: 25000000 INFO @ Sat, 03 Apr 2021 07:30:33: 17000000 INFO @ Sat, 03 Apr 2021 07:30:33: 29000000 INFO @ Sat, 03 Apr 2021 07:30:37: 26000000 INFO @ Sat, 03 Apr 2021 07:30:39: 18000000 INFO @ Sat, 03 Apr 2021 07:30:39: 30000000 INFO @ Sat, 03 Apr 2021 07:30:42: 27000000 INFO @ Sat, 03 Apr 2021 07:30:45: 19000000 INFO @ Sat, 03 Apr 2021 07:30:45: 31000000 INFO @ Sat, 03 Apr 2021 07:30:46: 28000000 INFO @ Sat, 03 Apr 2021 07:30:50: 20000000 INFO @ Sat, 03 Apr 2021 07:30:50: 32000000 INFO @ Sat, 03 Apr 2021 07:30:51: 29000000 INFO @ Sat, 03 Apr 2021 07:30:56: 21000000 INFO @ Sat, 03 Apr 2021 07:30:56: 33000000 INFO @ Sat, 03 Apr 2021 07:30:56: 30000000 INFO @ Sat, 03 Apr 2021 07:31:01: 34000000 INFO @ Sat, 03 Apr 2021 07:31:01: 22000000 INFO @ Sat, 03 Apr 2021 07:31:02: 31000000 INFO @ Sat, 03 Apr 2021 07:31:07: 32000000 INFO @ Sat, 03 Apr 2021 07:31:07: 35000000 INFO @ Sat, 03 Apr 2021 07:31:07: 23000000 INFO @ Sat, 03 Apr 2021 07:31:12: 33000000 INFO @ Sat, 03 Apr 2021 07:31:12: 36000000 INFO @ Sat, 03 Apr 2021 07:31:12: 24000000 INFO @ Sat, 03 Apr 2021 07:31:16: 34000000 INFO @ Sat, 03 Apr 2021 07:31:18: 37000000 INFO @ Sat, 03 Apr 2021 07:31:18: 25000000 INFO @ Sat, 03 Apr 2021 07:31:21: 35000000 INFO @ Sat, 03 Apr 2021 07:31:24: 38000000 INFO @ Sat, 03 Apr 2021 07:31:24: 26000000 INFO @ Sat, 03 Apr 2021 07:31:27: 36000000 INFO @ Sat, 03 Apr 2021 07:31:30: 39000000 INFO @ Sat, 03 Apr 2021 07:31:30: 27000000 INFO @ Sat, 03 Apr 2021 07:31:32: 37000000 INFO @ Sat, 03 Apr 2021 07:31:35: 40000000 INFO @ Sat, 03 Apr 2021 07:31:35: 28000000 INFO @ Sat, 03 Apr 2021 07:31:37: 38000000 INFO @ Sat, 03 Apr 2021 07:31:41: 41000000 INFO @ Sat, 03 Apr 2021 07:31:41: 29000000 INFO @ Sat, 03 Apr 2021 07:31:42: 39000000 INFO @ Sat, 03 Apr 2021 07:31:46: 42000000 INFO @ Sat, 03 Apr 2021 07:31:46: 30000000 INFO @ Sat, 03 Apr 2021 07:31:47: 40000000 INFO @ Sat, 03 Apr 2021 07:31:52: 41000000 INFO @ Sat, 03 Apr 2021 07:31:52: 31000000 INFO @ Sat, 03 Apr 2021 07:31:52: 43000000 INFO @ Sat, 03 Apr 2021 07:31:56: 42000000 INFO @ Sat, 03 Apr 2021 07:31:58: 32000000 INFO @ Sat, 03 Apr 2021 07:31:58: 44000000 INFO @ Sat, 03 Apr 2021 07:32:01: 43000000 INFO @ Sat, 03 Apr 2021 07:32:03: 33000000 INFO @ Sat, 03 Apr 2021 07:32:04: 45000000 INFO @ Sat, 03 Apr 2021 07:32:06: 44000000 INFO @ Sat, 03 Apr 2021 07:32:09: 34000000 INFO @ Sat, 03 Apr 2021 07:32:09: 46000000 INFO @ Sat, 03 Apr 2021 07:32:11: 45000000 INFO @ Sat, 03 Apr 2021 07:32:14: 35000000 INFO @ Sat, 03 Apr 2021 07:32:15: 47000000 INFO @ Sat, 03 Apr 2021 07:32:16: 46000000 INFO @ Sat, 03 Apr 2021 07:32:20: 36000000 INFO @ Sat, 03 Apr 2021 07:32:21: 48000000 INFO @ Sat, 03 Apr 2021 07:32:21: 47000000 INFO @ Sat, 03 Apr 2021 07:32:25: 37000000 INFO @ Sat, 03 Apr 2021 07:32:26: 48000000 INFO @ Sat, 03 Apr 2021 07:32:26: 49000000 INFO @ Sat, 03 Apr 2021 07:32:31: 49000000 INFO @ Sat, 03 Apr 2021 07:32:31: 38000000 INFO @ Sat, 03 Apr 2021 07:32:32: 50000000 INFO @ Sat, 03 Apr 2021 07:32:36: 50000000 INFO @ Sat, 03 Apr 2021 07:32:37: 39000000 INFO @ Sat, 03 Apr 2021 07:32:38: 51000000 INFO @ Sat, 03 Apr 2021 07:32:42: 51000000 INFO @ Sat, 03 Apr 2021 07:32:43: 40000000 INFO @ Sat, 03 Apr 2021 07:32:44: 52000000 INFO @ Sat, 03 Apr 2021 07:32:46: 52000000 INFO @ Sat, 03 Apr 2021 07:32:49: 41000000 INFO @ Sat, 03 Apr 2021 07:32:50: 53000000 INFO @ Sat, 03 Apr 2021 07:32:51: 53000000 INFO @ Sat, 03 Apr 2021 07:32:55: 42000000 INFO @ Sat, 03 Apr 2021 07:32:56: 54000000 INFO @ Sat, 03 Apr 2021 07:32:56: 54000000 INFO @ Sat, 03 Apr 2021 07:33:01: 43000000 INFO @ Sat, 03 Apr 2021 07:33:01: 55000000 INFO @ Sat, 03 Apr 2021 07:33:02: 55000000 INFO @ Sat, 03 Apr 2021 07:33:07: 44000000 INFO @ Sat, 03 Apr 2021 07:33:07: 56000000 INFO @ Sat, 03 Apr 2021 07:33:08: 56000000 INFO @ Sat, 03 Apr 2021 07:33:12: 57000000 INFO @ Sat, 03 Apr 2021 07:33:12: 45000000 INFO @ Sat, 03 Apr 2021 07:33:14: 57000000 INFO @ Sat, 03 Apr 2021 07:33:17: 58000000 INFO @ Sat, 03 Apr 2021 07:33:18: 46000000 INFO @ Sat, 03 Apr 2021 07:33:20: 58000000 INFO @ Sat, 03 Apr 2021 07:33:22: 59000000 INFO @ Sat, 03 Apr 2021 07:33:24: 47000000 INFO @ Sat, 03 Apr 2021 07:33:26: 59000000 INFO @ Sat, 03 Apr 2021 07:33:27: 60000000 INFO @ Sat, 03 Apr 2021 07:33:30: 48000000 INFO @ Sat, 03 Apr 2021 07:33:31: 60000000 INFO @ Sat, 03 Apr 2021 07:33:31: 61000000 INFO @ Sat, 03 Apr 2021 07:33:36: 49000000 INFO @ Sat, 03 Apr 2021 07:33:36: 62000000 INFO @ Sat, 03 Apr 2021 07:33:37: 61000000 INFO @ Sat, 03 Apr 2021 07:33:41: 63000000 INFO @ Sat, 03 Apr 2021 07:33:42: 50000000 INFO @ Sat, 03 Apr 2021 07:33:43: 62000000 INFO @ Sat, 03 Apr 2021 07:33:46: 64000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 03 Apr 2021 07:33:48: 51000000 INFO @ Sat, 03 Apr 2021 07:33:49: 63000000 INFO @ Sat, 03 Apr 2021 07:33:51: 65000000 INFO @ Sat, 03 Apr 2021 07:33:54: 52000000 INFO @ Sat, 03 Apr 2021 07:33:55: 64000000 INFO @ Sat, 03 Apr 2021 07:33:56: 66000000 INFO @ Sat, 03 Apr 2021 07:34:00: 53000000 INFO @ Sat, 03 Apr 2021 07:34:01: 67000000 INFO @ Sat, 03 Apr 2021 07:34:02: 65000000 INFO @ Sat, 03 Apr 2021 07:34:06: 68000000 INFO @ Sat, 03 Apr 2021 07:34:06: 54000000 INFO @ Sat, 03 Apr 2021 07:34:08: 66000000 INFO @ Sat, 03 Apr 2021 07:34:11: 69000000 INFO @ Sat, 03 Apr 2021 07:34:12: 55000000 INFO @ Sat, 03 Apr 2021 07:34:14: 67000000 INFO @ Sat, 03 Apr 2021 07:34:17: 70000000 INFO @ Sat, 03 Apr 2021 07:34:19: 56000000 INFO @ Sat, 03 Apr 2021 07:34:21: 68000000 INFO @ Sat, 03 Apr 2021 07:34:22: 71000000 INFO @ Sat, 03 Apr 2021 07:34:25: 57000000 INFO @ Sat, 03 Apr 2021 07:34:27: 72000000 INFO @ Sat, 03 Apr 2021 07:34:28: 69000000 INFO @ Sat, 03 Apr 2021 07:34:30: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 07:34:30: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 07:34:30: #1 total tags in treatment: 32406922 INFO @ Sat, 03 Apr 2021 07:34:30: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:34:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 07:34:31: #1 tags after filtering in treatment: 20951638 INFO @ Sat, 03 Apr 2021 07:34:31: #1 Redundant rate of treatment: 0.35 INFO @ Sat, 03 Apr 2021 07:34:31: #1 finished! INFO @ Sat, 03 Apr 2021 07:34:31: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:34:31: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 07:34:32: 58000000 INFO @ Sat, 03 Apr 2021 07:34:32: #2 number of paired peaks: 363 WARNING @ Sat, 03 Apr 2021 07:34:32: Fewer paired peaks (363) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 363 pairs to build model! INFO @ Sat, 03 Apr 2021 07:34:32: start model_add_line... INFO @ Sat, 03 Apr 2021 07:34:33: start X-correlation... INFO @ Sat, 03 Apr 2021 07:34:33: end of X-cor INFO @ Sat, 03 Apr 2021 07:34:33: #2 finished! INFO @ Sat, 03 Apr 2021 07:34:33: #2 predicted fragment length is 48 bps INFO @ Sat, 03 Apr 2021 07:34:33: #2 alternative fragment length(s) may be 4,48,580,585 bps INFO @ Sat, 03 Apr 2021 07:34:33: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX4518000/SRX4518000.10_model.r WARNING @ Sat, 03 Apr 2021 07:34:33: #2 Since the d (48) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 07:34:33: #2 You may need to consider one of the other alternative d(s): 4,48,580,585 WARNING @ Sat, 03 Apr 2021 07:34:33: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 07:34:33: #3 Call peaks... INFO @ Sat, 03 Apr 2021 07:34:33: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 07:34:34: 70000000 INFO @ Sat, 03 Apr 2021 07:34:39: 59000000 INFO @ Sat, 03 Apr 2021 07:34:41: 71000000 INFO @ Sat, 03 Apr 2021 07:34:45: 60000000 INFO @ Sat, 03 Apr 2021 07:34:47: 72000000 INFO @ Sat, 03 Apr 2021 07:34:52: 61000000 INFO @ Sat, 03 Apr 2021 07:34:52: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 07:34:52: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 07:34:52: #1 total tags in treatment: 32406922 INFO @ Sat, 03 Apr 2021 07:34:52: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:34:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 07:34:52: #1 tags after filtering in treatment: 20951638 INFO @ Sat, 03 Apr 2021 07:34:52: #1 Redundant rate of treatment: 0.35 INFO @ Sat, 03 Apr 2021 07:34:52: #1 finished! INFO @ Sat, 03 Apr 2021 07:34:52: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:34:52: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 07:34:54: #2 number of paired peaks: 363 WARNING @ Sat, 03 Apr 2021 07:34:54: Fewer paired peaks (363) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 363 pairs to build model! INFO @ Sat, 03 Apr 2021 07:34:54: start model_add_line... INFO @ Sat, 03 Apr 2021 07:34:54: start X-correlation... INFO @ Sat, 03 Apr 2021 07:34:54: end of X-cor INFO @ Sat, 03 Apr 2021 07:34:54: #2 finished! INFO @ Sat, 03 Apr 2021 07:34:54: #2 predicted fragment length is 48 bps INFO @ Sat, 03 Apr 2021 07:34:54: #2 alternative fragment length(s) may be 4,48,580,585 bps INFO @ Sat, 03 Apr 2021 07:34:54: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX4518000/SRX4518000.05_model.r WARNING @ Sat, 03 Apr 2021 07:34:54: #2 Since the d (48) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 07:34:54: #2 You may need to consider one of the other alternative d(s): 4,48,580,585 WARNING @ Sat, 03 Apr 2021 07:34:54: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 07:34:54: #3 Call peaks... INFO @ Sat, 03 Apr 2021 07:34:54: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 07:34:58: 62000000 INFO @ Sat, 03 Apr 2021 07:35:05: 63000000 INFO @ Sat, 03 Apr 2021 07:35:08: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 07:35:11: 64000000 INFO @ Sat, 03 Apr 2021 07:35:18: 65000000 BigWig に変換しました。 INFO @ Sat, 03 Apr 2021 07:35:24: 66000000 INFO @ Sat, 03 Apr 2021 07:35:27: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX4518000/SRX4518000.10_peaks.xls INFO @ Sat, 03 Apr 2021 07:35:27: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX4518000/SRX4518000.10_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 07:35:27: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX4518000/SRX4518000.10_summits.bed INFO @ Sat, 03 Apr 2021 07:35:27: Done! pass1 - making usageList (13 chroms): 2 millis pass2 - checking and writing primary data (4224 records, 4 fields): 7 millis CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 07:35:31: 67000000 INFO @ Sat, 03 Apr 2021 07:35:33: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 07:35:37: 68000000 INFO @ Sat, 03 Apr 2021 07:35:44: 69000000 INFO @ Sat, 03 Apr 2021 07:35:50: 70000000 INFO @ Sat, 03 Apr 2021 07:35:54: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX4518000/SRX4518000.05_peaks.xls INFO @ Sat, 03 Apr 2021 07:35:54: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX4518000/SRX4518000.05_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 07:35:54: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX4518000/SRX4518000.05_summits.bed INFO @ Sat, 03 Apr 2021 07:35:54: Done! pass1 - making usageList (14 chroms): 3 millis pass2 - checking and writing primary data (11950 records, 4 fields): 15 millis CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 07:35:57: 71000000 INFO @ Sat, 03 Apr 2021 07:36:04: 72000000 INFO @ Sat, 03 Apr 2021 07:36:08: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 07:36:08: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 07:36:08: #1 total tags in treatment: 32406922 INFO @ Sat, 03 Apr 2021 07:36:08: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:36:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 07:36:09: #1 tags after filtering in treatment: 20951638 INFO @ Sat, 03 Apr 2021 07:36:09: #1 Redundant rate of treatment: 0.35 INFO @ Sat, 03 Apr 2021 07:36:09: #1 finished! INFO @ Sat, 03 Apr 2021 07:36:09: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:36:09: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 07:36:10: #2 number of paired peaks: 363 WARNING @ Sat, 03 Apr 2021 07:36:10: Fewer paired peaks (363) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 363 pairs to build model! INFO @ Sat, 03 Apr 2021 07:36:10: start model_add_line... INFO @ Sat, 03 Apr 2021 07:36:10: start X-correlation... INFO @ Sat, 03 Apr 2021 07:36:10: end of X-cor INFO @ Sat, 03 Apr 2021 07:36:10: #2 finished! INFO @ Sat, 03 Apr 2021 07:36:10: #2 predicted fragment length is 48 bps INFO @ Sat, 03 Apr 2021 07:36:10: #2 alternative fragment length(s) may be 4,48,580,585 bps INFO @ Sat, 03 Apr 2021 07:36:10: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX4518000/SRX4518000.20_model.r WARNING @ Sat, 03 Apr 2021 07:36:10: #2 Since the d (48) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 07:36:10: #2 You may need to consider one of the other alternative d(s): 4,48,580,585 WARNING @ Sat, 03 Apr 2021 07:36:10: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 07:36:10: #3 Call peaks... INFO @ Sat, 03 Apr 2021 07:36:10: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 07:36:49: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 07:37:10: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX4518000/SRX4518000.20_peaks.xls INFO @ Sat, 03 Apr 2021 07:37:10: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX4518000/SRX4518000.20_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 07:37:10: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX4518000/SRX4518000.20_summits.bed INFO @ Sat, 03 Apr 2021 07:37:10: Done! pass1 - making usageList (9 chroms): 1 millis pass2 - checking and writing primary data (242 records, 4 fields): 2 millis CompletedMACS2peakCalling