Job ID = 12265220 SRX = SRX4517992 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 36515157 spots for SRR7655596/SRR7655596.sra Written 36515157 spots for SRR7655596/SRR7655596.sra fastq に変換しました。 bowtie でマッピング中... Your job 12265520 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:29:56 36515157 reads; of these: 36515157 (100.00%) were paired; of these: 3733279 (10.22%) aligned concordantly 0 times 31368873 (85.91%) aligned concordantly exactly 1 time 1413005 (3.87%) aligned concordantly >1 times ---- 3733279 pairs aligned concordantly 0 times; of these: 1761038 (47.17%) aligned discordantly 1 time ---- 1972241 pairs aligned 0 times concordantly or discordantly; of these: 3944482 mates make up the pairs; of these: 3094222 (78.44%) aligned 0 times 658003 (16.68%) aligned exactly 1 time 192257 (4.87%) aligned >1 times 95.76% overall alignment rate Time searching: 00:29:57 Overall time: 00:29:57 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 32 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 3015711 / 34184445 = 0.0882 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:17:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4517992/SRX4517992.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4517992/SRX4517992.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4517992/SRX4517992.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4517992/SRX4517992.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:17:06: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:17:06: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:17:15: 1000000 INFO @ Sat, 03 Apr 2021 07:17:24: 2000000 INFO @ Sat, 03 Apr 2021 07:17:33: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:17:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4517992/SRX4517992.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4517992/SRX4517992.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4517992/SRX4517992.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4517992/SRX4517992.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:17:36: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:17:36: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:17:42: 4000000 INFO @ Sat, 03 Apr 2021 07:17:45: 1000000 INFO @ Sat, 03 Apr 2021 07:17:51: 5000000 INFO @ Sat, 03 Apr 2021 07:17:54: 2000000 INFO @ Sat, 03 Apr 2021 07:18:00: 6000000 INFO @ Sat, 03 Apr 2021 07:18:03: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:18:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4517992/SRX4517992.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4517992/SRX4517992.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4517992/SRX4517992.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4517992/SRX4517992.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:18:06: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:18:06: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:18:10: 7000000 INFO @ Sat, 03 Apr 2021 07:18:12: 4000000 INFO @ Sat, 03 Apr 2021 07:18:16: 1000000 INFO @ Sat, 03 Apr 2021 07:18:19: 8000000 INFO @ Sat, 03 Apr 2021 07:18:22: 5000000 INFO @ Sat, 03 Apr 2021 07:18:25: 2000000 INFO @ Sat, 03 Apr 2021 07:18:28: 9000000 INFO @ Sat, 03 Apr 2021 07:18:31: 6000000 INFO @ Sat, 03 Apr 2021 07:18:35: 3000000 INFO @ Sat, 03 Apr 2021 07:18:37: 10000000 INFO @ Sat, 03 Apr 2021 07:18:40: 7000000 INFO @ Sat, 03 Apr 2021 07:18:44: 4000000 INFO @ Sat, 03 Apr 2021 07:18:46: 11000000 INFO @ Sat, 03 Apr 2021 07:18:49: 8000000 INFO @ Sat, 03 Apr 2021 07:18:54: 5000000 INFO @ Sat, 03 Apr 2021 07:18:55: 12000000 INFO @ Sat, 03 Apr 2021 07:18:59: 9000000 INFO @ Sat, 03 Apr 2021 07:19:03: 6000000 INFO @ Sat, 03 Apr 2021 07:19:03: 13000000 INFO @ Sat, 03 Apr 2021 07:19:09: 10000000 INFO @ Sat, 03 Apr 2021 07:19:13: 14000000 INFO @ Sat, 03 Apr 2021 07:19:13: 7000000 INFO @ Sat, 03 Apr 2021 07:19:17: 11000000 INFO @ Sat, 03 Apr 2021 07:19:22: 15000000 INFO @ Sat, 03 Apr 2021 07:19:22: 8000000 INFO @ Sat, 03 Apr 2021 07:19:26: 12000000 INFO @ Sat, 03 Apr 2021 07:19:30: 16000000 INFO @ Sat, 03 Apr 2021 07:19:32: 9000000 INFO @ Sat, 03 Apr 2021 07:19:35: 13000000 INFO @ Sat, 03 Apr 2021 07:19:39: 17000000 INFO @ Sat, 03 Apr 2021 07:19:41: 10000000 INFO @ Sat, 03 Apr 2021 07:19:44: 14000000 INFO @ Sat, 03 Apr 2021 07:19:48: 18000000 INFO @ Sat, 03 Apr 2021 07:19:51: 11000000 INFO @ Sat, 03 Apr 2021 07:19:54: 15000000 INFO @ Sat, 03 Apr 2021 07:19:57: 19000000 INFO @ Sat, 03 Apr 2021 07:20:00: 12000000 INFO @ Sat, 03 Apr 2021 07:20:03: 16000000 INFO @ Sat, 03 Apr 2021 07:20:06: 20000000 INFO @ Sat, 03 Apr 2021 07:20:09: 13000000 INFO @ Sat, 03 Apr 2021 07:20:12: 17000000 INFO @ Sat, 03 Apr 2021 07:20:15: 21000000 INFO @ Sat, 03 Apr 2021 07:20:19: 14000000 INFO @ Sat, 03 Apr 2021 07:20:22: 18000000 INFO @ Sat, 03 Apr 2021 07:20:23: 22000000 INFO @ Sat, 03 Apr 2021 07:20:28: 15000000 INFO @ Sat, 03 Apr 2021 07:20:31: 19000000 INFO @ Sat, 03 Apr 2021 07:20:32: 23000000 INFO @ Sat, 03 Apr 2021 07:20:38: 16000000 INFO @ Sat, 03 Apr 2021 07:20:40: 20000000 INFO @ Sat, 03 Apr 2021 07:20:41: 24000000 INFO @ Sat, 03 Apr 2021 07:20:47: 17000000 INFO @ Sat, 03 Apr 2021 07:20:50: 25000000 INFO @ Sat, 03 Apr 2021 07:20:50: 21000000 INFO @ Sat, 03 Apr 2021 07:20:57: 18000000 INFO @ Sat, 03 Apr 2021 07:20:59: 26000000 INFO @ Sat, 03 Apr 2021 07:21:00: 22000000 INFO @ Sat, 03 Apr 2021 07:21:06: 19000000 INFO @ Sat, 03 Apr 2021 07:21:07: 27000000 INFO @ Sat, 03 Apr 2021 07:21:09: 23000000 INFO @ Sat, 03 Apr 2021 07:21:15: 20000000 INFO @ Sat, 03 Apr 2021 07:21:16: 28000000 INFO @ Sat, 03 Apr 2021 07:21:18: 24000000 INFO @ Sat, 03 Apr 2021 07:21:24: 21000000 INFO @ Sat, 03 Apr 2021 07:21:25: 29000000 INFO @ Sat, 03 Apr 2021 07:21:28: 25000000 INFO @ Sat, 03 Apr 2021 07:21:34: 30000000 INFO @ Sat, 03 Apr 2021 07:21:34: 22000000 INFO @ Sat, 03 Apr 2021 07:21:37: 26000000 INFO @ Sat, 03 Apr 2021 07:21:42: 31000000 INFO @ Sat, 03 Apr 2021 07:21:43: 23000000 INFO @ Sat, 03 Apr 2021 07:21:46: 27000000 INFO @ Sat, 03 Apr 2021 07:21:51: 32000000 INFO @ Sat, 03 Apr 2021 07:21:53: 24000000 INFO @ Sat, 03 Apr 2021 07:21:56: 28000000 INFO @ Sat, 03 Apr 2021 07:22:00: 33000000 INFO @ Sat, 03 Apr 2021 07:22:02: 25000000 INFO @ Sat, 03 Apr 2021 07:22:05: 29000000 INFO @ Sat, 03 Apr 2021 07:22:09: 34000000 INFO @ Sat, 03 Apr 2021 07:22:11: 26000000 INFO @ Sat, 03 Apr 2021 07:22:14: 30000000 INFO @ Sat, 03 Apr 2021 07:22:18: 35000000 INFO @ Sat, 03 Apr 2021 07:22:21: 27000000 INFO @ Sat, 03 Apr 2021 07:22:24: 31000000 INFO @ Sat, 03 Apr 2021 07:22:26: 36000000 INFO @ Sat, 03 Apr 2021 07:22:30: 28000000 INFO @ Sat, 03 Apr 2021 07:22:33: 32000000 INFO @ Sat, 03 Apr 2021 07:22:35: 37000000 INFO @ Sat, 03 Apr 2021 07:22:39: 29000000 INFO @ Sat, 03 Apr 2021 07:22:43: 33000000 INFO @ Sat, 03 Apr 2021 07:22:44: 38000000 INFO @ Sat, 03 Apr 2021 07:22:48: 30000000 INFO @ Sat, 03 Apr 2021 07:22:52: 34000000 INFO @ Sat, 03 Apr 2021 07:22:53: 39000000 INFO @ Sat, 03 Apr 2021 07:22:57: 31000000 INFO @ Sat, 03 Apr 2021 07:23:01: 35000000 INFO @ Sat, 03 Apr 2021 07:23:02: 40000000 INFO @ Sat, 03 Apr 2021 07:23:07: 32000000 INFO @ Sat, 03 Apr 2021 07:23:10: 36000000 INFO @ Sat, 03 Apr 2021 07:23:11: 41000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 03 Apr 2021 07:23:16: 33000000 INFO @ Sat, 03 Apr 2021 07:23:19: 37000000 INFO @ Sat, 03 Apr 2021 07:23:20: 42000000 INFO @ Sat, 03 Apr 2021 07:23:25: 34000000 INFO @ Sat, 03 Apr 2021 07:23:28: 38000000 INFO @ Sat, 03 Apr 2021 07:23:28: 43000000 INFO @ Sat, 03 Apr 2021 07:23:34: 35000000 INFO @ Sat, 03 Apr 2021 07:23:37: 44000000 INFO @ Sat, 03 Apr 2021 07:23:37: 39000000 INFO @ Sat, 03 Apr 2021 07:23:44: 36000000 INFO @ Sat, 03 Apr 2021 07:23:45: 45000000 INFO @ Sat, 03 Apr 2021 07:23:46: 40000000 INFO @ Sat, 03 Apr 2021 07:23:53: 37000000 INFO @ Sat, 03 Apr 2021 07:23:54: 46000000 INFO @ Sat, 03 Apr 2021 07:23:56: 41000000 INFO @ Sat, 03 Apr 2021 07:24:02: 47000000 INFO @ Sat, 03 Apr 2021 07:24:02: 38000000 INFO @ Sat, 03 Apr 2021 07:24:06: 42000000 INFO @ Sat, 03 Apr 2021 07:24:11: 48000000 INFO @ Sat, 03 Apr 2021 07:24:11: 39000000 INFO @ Sat, 03 Apr 2021 07:24:15: 43000000 INFO @ Sat, 03 Apr 2021 07:24:19: 49000000 INFO @ Sat, 03 Apr 2021 07:24:21: 40000000 INFO @ Sat, 03 Apr 2021 07:24:24: 44000000 INFO @ Sat, 03 Apr 2021 07:24:28: 50000000 INFO @ Sat, 03 Apr 2021 07:24:31: 41000000 INFO @ Sat, 03 Apr 2021 07:24:34: 45000000 INFO @ Sat, 03 Apr 2021 07:24:37: 51000000 INFO @ Sat, 03 Apr 2021 07:24:40: 42000000 INFO @ Sat, 03 Apr 2021 07:24:44: 46000000 INFO @ Sat, 03 Apr 2021 07:24:45: 52000000 BigWig に変換しました。 INFO @ Sat, 03 Apr 2021 07:24:50: 43000000 INFO @ Sat, 03 Apr 2021 07:24:53: 47000000 INFO @ Sat, 03 Apr 2021 07:24:54: 53000000 INFO @ Sat, 03 Apr 2021 07:24:59: 44000000 INFO @ Sat, 03 Apr 2021 07:25:03: 48000000 INFO @ Sat, 03 Apr 2021 07:25:03: 54000000 INFO @ Sat, 03 Apr 2021 07:25:09: 45000000 INFO @ Sat, 03 Apr 2021 07:25:11: 55000000 INFO @ Sat, 03 Apr 2021 07:25:12: 49000000 INFO @ Sat, 03 Apr 2021 07:25:19: 46000000 INFO @ Sat, 03 Apr 2021 07:25:20: 56000000 INFO @ Sat, 03 Apr 2021 07:25:22: 50000000 INFO @ Sat, 03 Apr 2021 07:25:28: 47000000 INFO @ Sat, 03 Apr 2021 07:25:28: 57000000 INFO @ Sat, 03 Apr 2021 07:25:31: 51000000 INFO @ Sat, 03 Apr 2021 07:25:37: 58000000 INFO @ Sat, 03 Apr 2021 07:25:38: 48000000 INFO @ Sat, 03 Apr 2021 07:25:40: 52000000 INFO @ Sat, 03 Apr 2021 07:25:45: 59000000 INFO @ Sat, 03 Apr 2021 07:25:47: 49000000 INFO @ Sat, 03 Apr 2021 07:25:50: 53000000 INFO @ Sat, 03 Apr 2021 07:25:54: 60000000 INFO @ Sat, 03 Apr 2021 07:25:59: 50000000 INFO @ Sat, 03 Apr 2021 07:26:00: 54000000 INFO @ Sat, 03 Apr 2021 07:26:03: 61000000 INFO @ Sat, 03 Apr 2021 07:26:09: 55000000 INFO @ Sat, 03 Apr 2021 07:26:10: 51000000 INFO @ Sat, 03 Apr 2021 07:26:12: 62000000 INFO @ Sat, 03 Apr 2021 07:26:19: 56000000 INFO @ Sat, 03 Apr 2021 07:26:20: 52000000 INFO @ Sat, 03 Apr 2021 07:26:20: 63000000 INFO @ Sat, 03 Apr 2021 07:26:28: 57000000 INFO @ Sat, 03 Apr 2021 07:26:29: 53000000 INFO @ Sat, 03 Apr 2021 07:26:29: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 07:26:29: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 07:26:29: #1 total tags in treatment: 30009591 INFO @ Sat, 03 Apr 2021 07:26:29: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:26:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 07:26:29: #1 tags after filtering in treatment: 18992006 INFO @ Sat, 03 Apr 2021 07:26:29: #1 Redundant rate of treatment: 0.37 INFO @ Sat, 03 Apr 2021 07:26:29: #1 finished! INFO @ Sat, 03 Apr 2021 07:26:29: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:26:29: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 07:26:31: #2 number of paired peaks: 616 WARNING @ Sat, 03 Apr 2021 07:26:31: Fewer paired peaks (616) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 616 pairs to build model! INFO @ Sat, 03 Apr 2021 07:26:31: start model_add_line... INFO @ Sat, 03 Apr 2021 07:26:31: start X-correlation... INFO @ Sat, 03 Apr 2021 07:26:31: end of X-cor INFO @ Sat, 03 Apr 2021 07:26:31: #2 finished! INFO @ Sat, 03 Apr 2021 07:26:31: #2 predicted fragment length is 62 bps INFO @ Sat, 03 Apr 2021 07:26:31: #2 alternative fragment length(s) may be 62 bps INFO @ Sat, 03 Apr 2021 07:26:31: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX4517992/SRX4517992.05_model.r WARNING @ Sat, 03 Apr 2021 07:26:31: #2 Since the d (62) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 07:26:31: #2 You may need to consider one of the other alternative d(s): 62 WARNING @ Sat, 03 Apr 2021 07:26:31: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 07:26:31: #3 Call peaks... INFO @ Sat, 03 Apr 2021 07:26:31: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 07:26:37: 58000000 INFO @ Sat, 03 Apr 2021 07:26:38: 54000000 INFO @ Sat, 03 Apr 2021 07:26:46: 59000000 INFO @ Sat, 03 Apr 2021 07:26:47: 55000000 INFO @ Sat, 03 Apr 2021 07:26:56: 60000000 INFO @ Sat, 03 Apr 2021 07:26:56: 56000000 INFO @ Sat, 03 Apr 2021 07:27:05: 57000000 INFO @ Sat, 03 Apr 2021 07:27:05: 61000000 INFO @ Sat, 03 Apr 2021 07:27:14: 58000000 INFO @ Sat, 03 Apr 2021 07:27:14: 62000000 INFO @ Sat, 03 Apr 2021 07:27:20: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 07:27:23: 59000000 INFO @ Sat, 03 Apr 2021 07:27:23: 63000000 INFO @ Sat, 03 Apr 2021 07:27:32: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 07:27:32: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 07:27:32: #1 total tags in treatment: 30009591 INFO @ Sat, 03 Apr 2021 07:27:32: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:27:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 07:27:32: 60000000 INFO @ Sat, 03 Apr 2021 07:27:32: #1 tags after filtering in treatment: 18992006 INFO @ Sat, 03 Apr 2021 07:27:32: #1 Redundant rate of treatment: 0.37 INFO @ Sat, 03 Apr 2021 07:27:32: #1 finished! INFO @ Sat, 03 Apr 2021 07:27:32: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:27:32: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 07:27:34: #2 number of paired peaks: 616 WARNING @ Sat, 03 Apr 2021 07:27:34: Fewer paired peaks (616) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 616 pairs to build model! INFO @ Sat, 03 Apr 2021 07:27:34: start model_add_line... INFO @ Sat, 03 Apr 2021 07:27:34: start X-correlation... INFO @ Sat, 03 Apr 2021 07:27:34: end of X-cor INFO @ Sat, 03 Apr 2021 07:27:34: #2 finished! INFO @ Sat, 03 Apr 2021 07:27:34: #2 predicted fragment length is 62 bps INFO @ Sat, 03 Apr 2021 07:27:34: #2 alternative fragment length(s) may be 62 bps INFO @ Sat, 03 Apr 2021 07:27:34: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX4517992/SRX4517992.10_model.r WARNING @ Sat, 03 Apr 2021 07:27:34: #2 Since the d (62) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 07:27:34: #2 You may need to consider one of the other alternative d(s): 62 WARNING @ Sat, 03 Apr 2021 07:27:34: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 07:27:34: #3 Call peaks... INFO @ Sat, 03 Apr 2021 07:27:34: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 07:27:40: 61000000 INFO @ Sat, 03 Apr 2021 07:27:48: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX4517992/SRX4517992.05_peaks.xls INFO @ Sat, 03 Apr 2021 07:27:48: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX4517992/SRX4517992.05_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 07:27:48: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX4517992/SRX4517992.05_summits.bed INFO @ Sat, 03 Apr 2021 07:27:48: Done! pass1 - making usageList (13 chroms): 5 millis pass2 - checking and writing primary data (11892 records, 4 fields): 21 millis CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 07:27:49: 62000000 INFO @ Sat, 03 Apr 2021 07:27:57: 63000000 INFO @ Sat, 03 Apr 2021 07:28:05: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 07:28:05: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 07:28:05: #1 total tags in treatment: 30009591 INFO @ Sat, 03 Apr 2021 07:28:05: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:28:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 07:28:06: #1 tags after filtering in treatment: 18992006 INFO @ Sat, 03 Apr 2021 07:28:06: #1 Redundant rate of treatment: 0.37 INFO @ Sat, 03 Apr 2021 07:28:06: #1 finished! INFO @ Sat, 03 Apr 2021 07:28:06: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:28:06: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 07:28:08: #2 number of paired peaks: 616 WARNING @ Sat, 03 Apr 2021 07:28:08: Fewer paired peaks (616) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 616 pairs to build model! INFO @ Sat, 03 Apr 2021 07:28:08: start model_add_line... INFO @ Sat, 03 Apr 2021 07:28:08: start X-correlation... INFO @ Sat, 03 Apr 2021 07:28:08: end of X-cor INFO @ Sat, 03 Apr 2021 07:28:08: #2 finished! INFO @ Sat, 03 Apr 2021 07:28:08: #2 predicted fragment length is 62 bps INFO @ Sat, 03 Apr 2021 07:28:08: #2 alternative fragment length(s) may be 62 bps INFO @ Sat, 03 Apr 2021 07:28:08: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX4517992/SRX4517992.20_model.r WARNING @ Sat, 03 Apr 2021 07:28:08: #2 Since the d (62) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 07:28:08: #2 You may need to consider one of the other alternative d(s): 62 WARNING @ Sat, 03 Apr 2021 07:28:08: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 07:28:08: #3 Call peaks... INFO @ Sat, 03 Apr 2021 07:28:08: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 07:28:23: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 07:28:50: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX4517992/SRX4517992.10_peaks.xls INFO @ Sat, 03 Apr 2021 07:28:50: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX4517992/SRX4517992.10_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 07:28:51: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX4517992/SRX4517992.10_summits.bed INFO @ Sat, 03 Apr 2021 07:28:51: Done! pass1 - making usageList (13 chroms): 3 millis pass2 - checking and writing primary data (5113 records, 4 fields): 13 millis CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 07:28:56: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 07:29:22: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX4517992/SRX4517992.20_peaks.xls INFO @ Sat, 03 Apr 2021 07:29:22: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX4517992/SRX4517992.20_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 07:29:22: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX4517992/SRX4517992.20_summits.bed INFO @ Sat, 03 Apr 2021 07:29:22: Done! pass1 - making usageList (10 chroms): 1 millis pass2 - checking and writing primary data (706 records, 4 fields): 4 millis CompletedMACS2peakCalling