Job ID = 12265212 SRX = SRX4517986 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 35037705 spots for SRR7655590/SRR7655590.sra Written 35037705 spots for SRR7655590/SRR7655590.sra fastq に変換しました。 bowtie でマッピング中... Your job 12265512 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:29:00 35037705 reads; of these: 35037705 (100.00%) were paired; of these: 4271276 (12.19%) aligned concordantly 0 times 29388598 (83.88%) aligned concordantly exactly 1 time 1377831 (3.93%) aligned concordantly >1 times ---- 4271276 pairs aligned concordantly 0 times; of these: 2113869 (49.49%) aligned discordantly 1 time ---- 2157407 pairs aligned 0 times concordantly or discordantly; of these: 4314814 mates make up the pairs; of these: 3364523 (77.98%) aligned 0 times 706778 (16.38%) aligned exactly 1 time 243513 (5.64%) aligned >1 times 95.20% overall alignment rate Time searching: 00:29:00 Overall time: 00:29:00 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 28 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 2569463 / 32461142 = 0.0792 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:09:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4517986/SRX4517986.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4517986/SRX4517986.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4517986/SRX4517986.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4517986/SRX4517986.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:09:49: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:09:49: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:09:57: 1000000 INFO @ Sat, 03 Apr 2021 07:10:05: 2000000 INFO @ Sat, 03 Apr 2021 07:10:14: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:10:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4517986/SRX4517986.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4517986/SRX4517986.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4517986/SRX4517986.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4517986/SRX4517986.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:10:20: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:10:20: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:10:23: 4000000 INFO @ Sat, 03 Apr 2021 07:10:29: 1000000 INFO @ Sat, 03 Apr 2021 07:10:32: 5000000 INFO @ Sat, 03 Apr 2021 07:10:37: 2000000 INFO @ Sat, 03 Apr 2021 07:10:41: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:10:47: 3000000 INFO @ Sat, 03 Apr 2021 07:10:49: 7000000 INFO @ Sat, 03 Apr 2021 07:10:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4517986/SRX4517986.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4517986/SRX4517986.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4517986/SRX4517986.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4517986/SRX4517986.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:10:50: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:10:50: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:10:57: 4000000 INFO @ Sat, 03 Apr 2021 07:10:59: 8000000 INFO @ Sat, 03 Apr 2021 07:10:59: 1000000 INFO @ Sat, 03 Apr 2021 07:11:06: 5000000 INFO @ Sat, 03 Apr 2021 07:11:07: 9000000 INFO @ Sat, 03 Apr 2021 07:11:09: 2000000 INFO @ Sat, 03 Apr 2021 07:11:14: 6000000 INFO @ Sat, 03 Apr 2021 07:11:16: 10000000 INFO @ Sat, 03 Apr 2021 07:11:19: 3000000 INFO @ Sat, 03 Apr 2021 07:11:23: 7000000 INFO @ Sat, 03 Apr 2021 07:11:25: 11000000 INFO @ Sat, 03 Apr 2021 07:11:28: 4000000 INFO @ Sat, 03 Apr 2021 07:11:32: 8000000 INFO @ Sat, 03 Apr 2021 07:11:34: 12000000 INFO @ Sat, 03 Apr 2021 07:11:37: 5000000 INFO @ Sat, 03 Apr 2021 07:11:40: 9000000 INFO @ Sat, 03 Apr 2021 07:11:43: 13000000 INFO @ Sat, 03 Apr 2021 07:11:46: 6000000 INFO @ Sat, 03 Apr 2021 07:11:50: 10000000 INFO @ Sat, 03 Apr 2021 07:11:52: 14000000 INFO @ Sat, 03 Apr 2021 07:11:55: 7000000 INFO @ Sat, 03 Apr 2021 07:11:58: 11000000 INFO @ Sat, 03 Apr 2021 07:12:01: 15000000 INFO @ Sat, 03 Apr 2021 07:12:03: 8000000 INFO @ Sat, 03 Apr 2021 07:12:08: 12000000 INFO @ Sat, 03 Apr 2021 07:12:10: 16000000 INFO @ Sat, 03 Apr 2021 07:12:12: 9000000 INFO @ Sat, 03 Apr 2021 07:12:17: 13000000 INFO @ Sat, 03 Apr 2021 07:12:19: 17000000 INFO @ Sat, 03 Apr 2021 07:12:21: 10000000 INFO @ Sat, 03 Apr 2021 07:12:27: 14000000 INFO @ Sat, 03 Apr 2021 07:12:28: 18000000 INFO @ Sat, 03 Apr 2021 07:12:30: 11000000 INFO @ Sat, 03 Apr 2021 07:12:36: 15000000 INFO @ Sat, 03 Apr 2021 07:12:36: 19000000 INFO @ Sat, 03 Apr 2021 07:12:39: 12000000 INFO @ Sat, 03 Apr 2021 07:12:44: 16000000 INFO @ Sat, 03 Apr 2021 07:12:45: 20000000 INFO @ Sat, 03 Apr 2021 07:12:48: 13000000 INFO @ Sat, 03 Apr 2021 07:12:53: 17000000 INFO @ Sat, 03 Apr 2021 07:12:54: 21000000 INFO @ Sat, 03 Apr 2021 07:12:57: 14000000 INFO @ Sat, 03 Apr 2021 07:13:02: 18000000 INFO @ Sat, 03 Apr 2021 07:13:03: 22000000 INFO @ Sat, 03 Apr 2021 07:13:05: 15000000 INFO @ Sat, 03 Apr 2021 07:13:11: 19000000 INFO @ Sat, 03 Apr 2021 07:13:11: 23000000 INFO @ Sat, 03 Apr 2021 07:13:14: 16000000 INFO @ Sat, 03 Apr 2021 07:13:19: 20000000 INFO @ Sat, 03 Apr 2021 07:13:21: 24000000 INFO @ Sat, 03 Apr 2021 07:13:23: 17000000 INFO @ Sat, 03 Apr 2021 07:13:28: 21000000 INFO @ Sat, 03 Apr 2021 07:13:30: 25000000 INFO @ Sat, 03 Apr 2021 07:13:31: 18000000 INFO @ Sat, 03 Apr 2021 07:13:36: 22000000 INFO @ Sat, 03 Apr 2021 07:13:39: 26000000 INFO @ Sat, 03 Apr 2021 07:13:41: 19000000 INFO @ Sat, 03 Apr 2021 07:13:45: 23000000 INFO @ Sat, 03 Apr 2021 07:13:49: 20000000 INFO @ Sat, 03 Apr 2021 07:13:50: 27000000 INFO @ Sat, 03 Apr 2021 07:13:54: 24000000 INFO @ Sat, 03 Apr 2021 07:13:58: 21000000 INFO @ Sat, 03 Apr 2021 07:13:59: 28000000 INFO @ Sat, 03 Apr 2021 07:14:02: 25000000 INFO @ Sat, 03 Apr 2021 07:14:07: 22000000 INFO @ Sat, 03 Apr 2021 07:14:08: 29000000 INFO @ Sat, 03 Apr 2021 07:14:10: 26000000 INFO @ Sat, 03 Apr 2021 07:14:17: 23000000 INFO @ Sat, 03 Apr 2021 07:14:17: 30000000 INFO @ Sat, 03 Apr 2021 07:14:19: 27000000 INFO @ Sat, 03 Apr 2021 07:14:25: 24000000 INFO @ Sat, 03 Apr 2021 07:14:26: 31000000 INFO @ Sat, 03 Apr 2021 07:14:29: 28000000 INFO @ Sat, 03 Apr 2021 07:14:35: 25000000 INFO @ Sat, 03 Apr 2021 07:14:36: 32000000 INFO @ Sat, 03 Apr 2021 07:14:38: 29000000 INFO @ Sat, 03 Apr 2021 07:14:43: 26000000 INFO @ Sat, 03 Apr 2021 07:14:45: 33000000 INFO @ Sat, 03 Apr 2021 07:14:47: 30000000 INFO @ Sat, 03 Apr 2021 07:14:52: 27000000 INFO @ Sat, 03 Apr 2021 07:14:54: 34000000 INFO @ Sat, 03 Apr 2021 07:14:56: 31000000 INFO @ Sat, 03 Apr 2021 07:15:00: 28000000 INFO @ Sat, 03 Apr 2021 07:15:03: 35000000 INFO @ Sat, 03 Apr 2021 07:15:05: 32000000 INFO @ Sat, 03 Apr 2021 07:15:10: 29000000 INFO @ Sat, 03 Apr 2021 07:15:12: 36000000 INFO @ Sat, 03 Apr 2021 07:15:15: 33000000 INFO @ Sat, 03 Apr 2021 07:15:19: 30000000 INFO @ Sat, 03 Apr 2021 07:15:22: 37000000 INFO @ Sat, 03 Apr 2021 07:15:23: 34000000 INFO @ Sat, 03 Apr 2021 07:15:28: 31000000 INFO @ Sat, 03 Apr 2021 07:15:30: 38000000 INFO @ Sat, 03 Apr 2021 07:15:32: 35000000 INFO @ Sat, 03 Apr 2021 07:15:37: 32000000 INFO @ Sat, 03 Apr 2021 07:15:38: 39000000 INFO @ Sat, 03 Apr 2021 07:15:41: 36000000 INFO @ Sat, 03 Apr 2021 07:15:47: 33000000 INFO @ Sat, 03 Apr 2021 07:15:47: 40000000 INFO @ Sat, 03 Apr 2021 07:15:48: 37000000 INFO @ Sat, 03 Apr 2021 07:15:56: 34000000 INFO @ Sat, 03 Apr 2021 07:15:56: 38000000 INFO @ Sat, 03 Apr 2021 07:15:57: 41000000 INFO @ Sat, 03 Apr 2021 07:16:04: 35000000 INFO @ Sat, 03 Apr 2021 07:16:04: 39000000 INFO @ Sat, 03 Apr 2021 07:16:05: 42000000 INFO @ Sat, 03 Apr 2021 07:16:12: 40000000 INFO @ Sat, 03 Apr 2021 07:16:12: 36000000 INFO @ Sat, 03 Apr 2021 07:16:12: 43000000 INFO @ Sat, 03 Apr 2021 07:16:20: 41000000 INFO @ Sat, 03 Apr 2021 07:16:20: 44000000 INFO @ Sat, 03 Apr 2021 07:16:21: 37000000 INFO @ Sat, 03 Apr 2021 07:16:28: 42000000 INFO @ Sat, 03 Apr 2021 07:16:29: 45000000 INFO @ Sat, 03 Apr 2021 07:16:29: 38000000 INFO @ Sat, 03 Apr 2021 07:16:36: 43000000 INFO @ Sat, 03 Apr 2021 07:16:37: 39000000 INFO @ Sat, 03 Apr 2021 07:16:38: 46000000 INFO @ Sat, 03 Apr 2021 07:16:45: 44000000 INFO @ Sat, 03 Apr 2021 07:16:46: 40000000 INFO @ Sat, 03 Apr 2021 07:16:46: 47000000 INFO @ Sat, 03 Apr 2021 07:16:53: 45000000 INFO @ Sat, 03 Apr 2021 07:16:54: 48000000 INFO @ Sat, 03 Apr 2021 07:16:54: 41000000 INFO @ Sat, 03 Apr 2021 07:17:00: 46000000 INFO @ Sat, 03 Apr 2021 07:17:02: 49000000 INFO @ Sat, 03 Apr 2021 07:17:02: 42000000 INFO @ Sat, 03 Apr 2021 07:17:08: 47000000 INFO @ Sat, 03 Apr 2021 07:17:10: 50000000 INFO @ Sat, 03 Apr 2021 07:17:10: 43000000 INFO @ Sat, 03 Apr 2021 07:17:15: 48000000 INFO @ Sat, 03 Apr 2021 07:17:17: 51000000 INFO @ Sat, 03 Apr 2021 07:17:17: 44000000 INFO @ Sat, 03 Apr 2021 07:17:22: 49000000 INFO @ Sat, 03 Apr 2021 07:17:24: 52000000 INFO @ Sat, 03 Apr 2021 07:17:25: 45000000 INFO @ Sat, 03 Apr 2021 07:17:29: 50000000 INFO @ Sat, 03 Apr 2021 07:17:32: 53000000 INFO @ Sat, 03 Apr 2021 07:17:32: 46000000 INFO @ Sat, 03 Apr 2021 07:17:36: 51000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 03 Apr 2021 07:17:39: 54000000 INFO @ Sat, 03 Apr 2021 07:17:39: 47000000 INFO @ Sat, 03 Apr 2021 07:17:43: 52000000 INFO @ Sat, 03 Apr 2021 07:17:46: 55000000 INFO @ Sat, 03 Apr 2021 07:17:46: 48000000 INFO @ Sat, 03 Apr 2021 07:17:50: 53000000 INFO @ Sat, 03 Apr 2021 07:17:54: 56000000 INFO @ Sat, 03 Apr 2021 07:17:54: 49000000 INFO @ Sat, 03 Apr 2021 07:17:57: 54000000 INFO @ Sat, 03 Apr 2021 07:18:01: 50000000 INFO @ Sat, 03 Apr 2021 07:18:01: 57000000 INFO @ Sat, 03 Apr 2021 07:18:04: 55000000 INFO @ Sat, 03 Apr 2021 07:18:08: 51000000 INFO @ Sat, 03 Apr 2021 07:18:08: 58000000 INFO @ Sat, 03 Apr 2021 07:18:11: 56000000 INFO @ Sat, 03 Apr 2021 07:18:15: 59000000 INFO @ Sat, 03 Apr 2021 07:18:16: 52000000 INFO @ Sat, 03 Apr 2021 07:18:19: 57000000 INFO @ Sat, 03 Apr 2021 07:18:23: 60000000 INFO @ Sat, 03 Apr 2021 07:18:23: 53000000 INFO @ Sat, 03 Apr 2021 07:18:27: 58000000 INFO @ Sat, 03 Apr 2021 07:18:30: 61000000 INFO @ Sat, 03 Apr 2021 07:18:30: 54000000 INFO @ Sat, 03 Apr 2021 07:18:34: 59000000 INFO @ Sat, 03 Apr 2021 07:18:34: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 07:18:34: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 07:18:34: #1 total tags in treatment: 28469689 INFO @ Sat, 03 Apr 2021 07:18:34: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:18:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 07:18:35: #1 tags after filtering in treatment: 19042355 INFO @ Sat, 03 Apr 2021 07:18:35: #1 Redundant rate of treatment: 0.33 INFO @ Sat, 03 Apr 2021 07:18:35: #1 finished! INFO @ Sat, 03 Apr 2021 07:18:35: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:18:35: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 07:18:36: #2 number of paired peaks: 303 WARNING @ Sat, 03 Apr 2021 07:18:36: Fewer paired peaks (303) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 303 pairs to build model! INFO @ Sat, 03 Apr 2021 07:18:36: start model_add_line... INFO @ Sat, 03 Apr 2021 07:18:37: start X-correlation... INFO @ Sat, 03 Apr 2021 07:18:37: end of X-cor INFO @ Sat, 03 Apr 2021 07:18:37: #2 finished! INFO @ Sat, 03 Apr 2021 07:18:37: #2 predicted fragment length is 46 bps INFO @ Sat, 03 Apr 2021 07:18:37: #2 alternative fragment length(s) may be 46,587 bps INFO @ Sat, 03 Apr 2021 07:18:37: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX4517986/SRX4517986.05_model.r WARNING @ Sat, 03 Apr 2021 07:18:37: #2 Since the d (46) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 07:18:37: #2 You may need to consider one of the other alternative d(s): 46,587 WARNING @ Sat, 03 Apr 2021 07:18:37: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 07:18:37: #3 Call peaks... INFO @ Sat, 03 Apr 2021 07:18:37: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 07:18:37: 55000000 INFO @ Sat, 03 Apr 2021 07:18:41: 60000000 INFO @ Sat, 03 Apr 2021 07:18:44: 56000000 INFO @ Sat, 03 Apr 2021 07:18:48: 61000000 INFO @ Sat, 03 Apr 2021 07:18:51: 57000000 INFO @ Sat, 03 Apr 2021 07:18:51: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 07:18:51: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 07:18:51: #1 total tags in treatment: 28469689 INFO @ Sat, 03 Apr 2021 07:18:51: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:18:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 07:18:52: #1 tags after filtering in treatment: 19042355 INFO @ Sat, 03 Apr 2021 07:18:52: #1 Redundant rate of treatment: 0.33 INFO @ Sat, 03 Apr 2021 07:18:52: #1 finished! INFO @ Sat, 03 Apr 2021 07:18:52: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:18:52: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 07:18:53: #2 number of paired peaks: 303 WARNING @ Sat, 03 Apr 2021 07:18:53: Fewer paired peaks (303) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 303 pairs to build model! INFO @ Sat, 03 Apr 2021 07:18:53: start model_add_line... INFO @ Sat, 03 Apr 2021 07:18:54: start X-correlation... INFO @ Sat, 03 Apr 2021 07:18:54: end of X-cor INFO @ Sat, 03 Apr 2021 07:18:54: #2 finished! INFO @ Sat, 03 Apr 2021 07:18:54: #2 predicted fragment length is 46 bps INFO @ Sat, 03 Apr 2021 07:18:54: #2 alternative fragment length(s) may be 46,587 bps INFO @ Sat, 03 Apr 2021 07:18:54: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX4517986/SRX4517986.10_model.r WARNING @ Sat, 03 Apr 2021 07:18:54: #2 Since the d (46) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 07:18:54: #2 You may need to consider one of the other alternative d(s): 46,587 WARNING @ Sat, 03 Apr 2021 07:18:54: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 07:18:54: #3 Call peaks... INFO @ Sat, 03 Apr 2021 07:18:54: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 07:18:58: 58000000 INFO @ Sat, 03 Apr 2021 07:19:05: 59000000 BigWig に変換しました。 INFO @ Sat, 03 Apr 2021 07:19:11: 60000000 INFO @ Sat, 03 Apr 2021 07:19:13: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 07:19:18: 61000000 INFO @ Sat, 03 Apr 2021 07:19:22: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 07:19:22: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 07:19:22: #1 total tags in treatment: 28469689 INFO @ Sat, 03 Apr 2021 07:19:22: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:19:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 07:19:23: #1 tags after filtering in treatment: 19042355 INFO @ Sat, 03 Apr 2021 07:19:23: #1 Redundant rate of treatment: 0.33 INFO @ Sat, 03 Apr 2021 07:19:23: #1 finished! INFO @ Sat, 03 Apr 2021 07:19:23: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:19:23: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 07:19:24: #2 number of paired peaks: 303 WARNING @ Sat, 03 Apr 2021 07:19:24: Fewer paired peaks (303) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 303 pairs to build model! INFO @ Sat, 03 Apr 2021 07:19:24: start model_add_line... INFO @ Sat, 03 Apr 2021 07:19:25: start X-correlation... INFO @ Sat, 03 Apr 2021 07:19:25: end of X-cor INFO @ Sat, 03 Apr 2021 07:19:25: #2 finished! INFO @ Sat, 03 Apr 2021 07:19:25: #2 predicted fragment length is 46 bps INFO @ Sat, 03 Apr 2021 07:19:25: #2 alternative fragment length(s) may be 46,587 bps INFO @ Sat, 03 Apr 2021 07:19:25: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX4517986/SRX4517986.20_model.r WARNING @ Sat, 03 Apr 2021 07:19:25: #2 Since the d (46) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 07:19:25: #2 You may need to consider one of the other alternative d(s): 46,587 WARNING @ Sat, 03 Apr 2021 07:19:25: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 07:19:25: #3 Call peaks... INFO @ Sat, 03 Apr 2021 07:19:25: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 07:19:28: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 07:19:32: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX4517986/SRX4517986.05_peaks.xls INFO @ Sat, 03 Apr 2021 07:19:32: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX4517986/SRX4517986.05_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 07:19:32: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX4517986/SRX4517986.05_summits.bed INFO @ Sat, 03 Apr 2021 07:19:32: Done! pass1 - making usageList (13 chroms): 2 millis pass2 - checking and writing primary data (9204 records, 4 fields): 13 millis CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 07:19:46: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX4517986/SRX4517986.10_peaks.xls INFO @ Sat, 03 Apr 2021 07:19:46: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX4517986/SRX4517986.10_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 07:19:46: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX4517986/SRX4517986.10_summits.bed INFO @ Sat, 03 Apr 2021 07:19:46: Done! pass1 - making usageList (12 chroms): 1 millis pass2 - checking and writing primary data (2484 records, 4 fields): 17 millis CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 07:19:59: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 07:20:17: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX4517986/SRX4517986.20_peaks.xls INFO @ Sat, 03 Apr 2021 07:20:17: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX4517986/SRX4517986.20_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 07:20:17: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX4517986/SRX4517986.20_summits.bed INFO @ Sat, 03 Apr 2021 07:20:17: Done! pass1 - making usageList (7 chroms): 0 millis pass2 - checking and writing primary data (91 records, 4 fields): 2 millis CompletedMACS2peakCalling