Job ID = 6528089 SRX = SRX450798 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:16:51 prefetch.2.10.7: 1) Downloading 'SRR1145615'... 2020-06-29T14:16:51 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:28:41 prefetch.2.10.7: HTTPS download failed 2020-06-29T14:28:41 prefetch.2.10.7: 1) failed to download SRR1145615 2020-06-29T14:28:55 prefetch.2.10.7: 1) Downloading 'SRR1145615'... 2020-06-29T14:28:55 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:28:55 prefetch.2.10.7: Continue download of 'SRR1145615' from 1709965347 2020-06-29T14:29:03 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:29:03 prefetch.2.10.7: 1) 'SRR1145615' was downloaded successfully Read 27371657 spots for SRR1145615/SRR1145615.sra Written 27371657 spots for SRR1145615/SRR1145615.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:12:12 27371657 reads; of these: 27371657 (100.00%) were unpaired; of these: 4598993 (16.80%) aligned 0 times 19272983 (70.41%) aligned exactly 1 time 3499681 (12.79%) aligned >1 times 83.20% overall alignment rate Time searching: 00:12:12 Overall time: 00:12:12 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 6012704 / 22772664 = 0.2640 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:03:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX450798/SRX450798.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX450798/SRX450798.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX450798/SRX450798.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX450798/SRX450798.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:03:44: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:03:44: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:03:50: 1000000 INFO @ Tue, 30 Jun 2020 00:03:57: 2000000 INFO @ Tue, 30 Jun 2020 00:04:03: 3000000 INFO @ Tue, 30 Jun 2020 00:04:09: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:04:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX450798/SRX450798.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX450798/SRX450798.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX450798/SRX450798.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX450798/SRX450798.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:04:15: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:04:15: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:04:16: 5000000 INFO @ Tue, 30 Jun 2020 00:04:22: 1000000 INFO @ Tue, 30 Jun 2020 00:04:23: 6000000 INFO @ Tue, 30 Jun 2020 00:04:29: 2000000 INFO @ Tue, 30 Jun 2020 00:04:30: 7000000 INFO @ Tue, 30 Jun 2020 00:04:36: 3000000 INFO @ Tue, 30 Jun 2020 00:04:37: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:04:43: 4000000 INFO @ Tue, 30 Jun 2020 00:04:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX450798/SRX450798.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX450798/SRX450798.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX450798/SRX450798.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX450798/SRX450798.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:04:44: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:04:44: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:04:44: 9000000 INFO @ Tue, 30 Jun 2020 00:04:50: 5000000 INFO @ Tue, 30 Jun 2020 00:04:51: 1000000 INFO @ Tue, 30 Jun 2020 00:04:51: 10000000 INFO @ Tue, 30 Jun 2020 00:04:57: 6000000 INFO @ Tue, 30 Jun 2020 00:04:58: 2000000 INFO @ Tue, 30 Jun 2020 00:04:58: 11000000 INFO @ Tue, 30 Jun 2020 00:05:04: 7000000 INFO @ Tue, 30 Jun 2020 00:05:05: 3000000 INFO @ Tue, 30 Jun 2020 00:05:05: 12000000 INFO @ Tue, 30 Jun 2020 00:05:11: 8000000 INFO @ Tue, 30 Jun 2020 00:05:11: 4000000 INFO @ Tue, 30 Jun 2020 00:05:12: 13000000 INFO @ Tue, 30 Jun 2020 00:05:17: 9000000 INFO @ Tue, 30 Jun 2020 00:05:18: 5000000 INFO @ Tue, 30 Jun 2020 00:05:19: 14000000 INFO @ Tue, 30 Jun 2020 00:05:24: 10000000 INFO @ Tue, 30 Jun 2020 00:05:25: 6000000 INFO @ Tue, 30 Jun 2020 00:05:26: 15000000 INFO @ Tue, 30 Jun 2020 00:05:31: 11000000 INFO @ Tue, 30 Jun 2020 00:05:32: 7000000 INFO @ Tue, 30 Jun 2020 00:05:33: 16000000 INFO @ Tue, 30 Jun 2020 00:05:38: #1 tag size is determined as 100 bps INFO @ Tue, 30 Jun 2020 00:05:38: #1 tag size = 100 INFO @ Tue, 30 Jun 2020 00:05:38: #1 total tags in treatment: 16759960 INFO @ Tue, 30 Jun 2020 00:05:38: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:05:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:05:38: 12000000 INFO @ Tue, 30 Jun 2020 00:05:38: #1 tags after filtering in treatment: 16759960 INFO @ Tue, 30 Jun 2020 00:05:38: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:05:38: #1 finished! INFO @ Tue, 30 Jun 2020 00:05:38: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:05:38: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:05:39: 8000000 INFO @ Tue, 30 Jun 2020 00:05:39: #2 number of paired peaks: 35 WARNING @ Tue, 30 Jun 2020 00:05:39: Too few paired peaks (35) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:05:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX450798/SRX450798.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX450798/SRX450798.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX450798/SRX450798.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX450798/SRX450798.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:05:45: 13000000 INFO @ Tue, 30 Jun 2020 00:05:46: 9000000 INFO @ Tue, 30 Jun 2020 00:05:52: 14000000 INFO @ Tue, 30 Jun 2020 00:05:53: 10000000 INFO @ Tue, 30 Jun 2020 00:05:59: 15000000 INFO @ Tue, 30 Jun 2020 00:06:00: 11000000 INFO @ Tue, 30 Jun 2020 00:06:06: 16000000 INFO @ Tue, 30 Jun 2020 00:06:07: 12000000 INFO @ Tue, 30 Jun 2020 00:06:11: #1 tag size is determined as 100 bps INFO @ Tue, 30 Jun 2020 00:06:11: #1 tag size = 100 INFO @ Tue, 30 Jun 2020 00:06:11: #1 total tags in treatment: 16759960 INFO @ Tue, 30 Jun 2020 00:06:11: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:06:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:06:11: #1 tags after filtering in treatment: 16759960 INFO @ Tue, 30 Jun 2020 00:06:11: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:06:11: #1 finished! INFO @ Tue, 30 Jun 2020 00:06:11: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:06:11: #2 looking for paired plus/minus strand peaks... BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 00:06:13: #2 number of paired peaks: 35 WARNING @ Tue, 30 Jun 2020 00:06:13: Too few paired peaks (35) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:06:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX450798/SRX450798.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX450798/SRX450798.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX450798/SRX450798.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX450798/SRX450798.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:06:14: 13000000 INFO @ Tue, 30 Jun 2020 00:06:21: 14000000 INFO @ Tue, 30 Jun 2020 00:06:27: 15000000 INFO @ Tue, 30 Jun 2020 00:06:34: 16000000 INFO @ Tue, 30 Jun 2020 00:06:39: #1 tag size is determined as 100 bps INFO @ Tue, 30 Jun 2020 00:06:39: #1 tag size = 100 INFO @ Tue, 30 Jun 2020 00:06:39: #1 total tags in treatment: 16759960 INFO @ Tue, 30 Jun 2020 00:06:39: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:06:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:06:39: #1 tags after filtering in treatment: 16759960 INFO @ Tue, 30 Jun 2020 00:06:39: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:06:39: #1 finished! INFO @ Tue, 30 Jun 2020 00:06:39: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:06:39: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:06:40: #2 number of paired peaks: 35 WARNING @ Tue, 30 Jun 2020 00:06:40: Too few paired peaks (35) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:06:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX450798/SRX450798.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX450798/SRX450798.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX450798/SRX450798.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX450798/SRX450798.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。