Job ID = 1295771 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 16,190,346 reads read : 16,190,346 reads written : 16,190,346 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:58 16190346 reads; of these: 16190346 (100.00%) were unpaired; of these: 4642164 (28.67%) aligned 0 times 10736150 (66.31%) aligned exactly 1 time 812032 (5.02%) aligned >1 times 71.33% overall alignment rate Time searching: 00:02:58 Overall time: 00:02:58 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 1202655 / 11548182 = 0.1041 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 15:36:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX450795/SRX450795.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX450795/SRX450795.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX450795/SRX450795.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX450795/SRX450795.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 15:36:25: #1 read tag files... INFO @ Mon, 03 Jun 2019 15:36:25: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 15:36:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX450795/SRX450795.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX450795/SRX450795.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX450795/SRX450795.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX450795/SRX450795.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 15:36:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX450795/SRX450795.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX450795/SRX450795.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX450795/SRX450795.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX450795/SRX450795.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 15:36:25: #1 read tag files... INFO @ Mon, 03 Jun 2019 15:36:25: #1 read tag files... INFO @ Mon, 03 Jun 2019 15:36:25: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 15:36:25: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 15:36:34: 1000000 INFO @ Mon, 03 Jun 2019 15:36:34: 1000000 INFO @ Mon, 03 Jun 2019 15:36:34: 1000000 INFO @ Mon, 03 Jun 2019 15:36:43: 2000000 INFO @ Mon, 03 Jun 2019 15:36:44: 2000000 INFO @ Mon, 03 Jun 2019 15:36:44: 2000000 INFO @ Mon, 03 Jun 2019 15:36:53: 3000000 INFO @ Mon, 03 Jun 2019 15:36:53: 3000000 INFO @ Mon, 03 Jun 2019 15:36:53: 3000000 INFO @ Mon, 03 Jun 2019 15:37:01: 4000000 INFO @ Mon, 03 Jun 2019 15:37:01: 4000000 INFO @ Mon, 03 Jun 2019 15:37:02: 4000000 INFO @ Mon, 03 Jun 2019 15:37:09: 5000000 INFO @ Mon, 03 Jun 2019 15:37:09: 5000000 INFO @ Mon, 03 Jun 2019 15:37:10: 5000000 INFO @ Mon, 03 Jun 2019 15:37:16: 6000000 INFO @ Mon, 03 Jun 2019 15:37:17: 6000000 INFO @ Mon, 03 Jun 2019 15:37:18: 6000000 INFO @ Mon, 03 Jun 2019 15:37:24: 7000000 INFO @ Mon, 03 Jun 2019 15:37:25: 7000000 INFO @ Mon, 03 Jun 2019 15:37:27: 7000000 INFO @ Mon, 03 Jun 2019 15:37:32: 8000000 INFO @ Mon, 03 Jun 2019 15:37:32: 8000000 INFO @ Mon, 03 Jun 2019 15:37:35: 8000000 INFO @ Mon, 03 Jun 2019 15:37:39: 9000000 INFO @ Mon, 03 Jun 2019 15:37:40: 9000000 INFO @ Mon, 03 Jun 2019 15:37:43: 9000000 INFO @ Mon, 03 Jun 2019 15:37:47: 10000000 INFO @ Mon, 03 Jun 2019 15:37:48: 10000000 INFO @ Mon, 03 Jun 2019 15:37:50: #1 tag size is determined as 41 bps INFO @ Mon, 03 Jun 2019 15:37:50: #1 tag size = 41 INFO @ Mon, 03 Jun 2019 15:37:50: #1 total tags in treatment: 10345527 INFO @ Mon, 03 Jun 2019 15:37:50: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 15:37:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 15:37:50: #1 tags after filtering in treatment: 10345527 INFO @ Mon, 03 Jun 2019 15:37:50: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 15:37:50: #1 finished! INFO @ Mon, 03 Jun 2019 15:37:50: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 15:37:50: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 15:37:50: #1 tag size is determined as 41 bps INFO @ Mon, 03 Jun 2019 15:37:50: #1 tag size = 41 INFO @ Mon, 03 Jun 2019 15:37:50: #1 total tags in treatment: 10345527 INFO @ Mon, 03 Jun 2019 15:37:50: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 15:37:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 15:37:51: #1 tags after filtering in treatment: 10345527 INFO @ Mon, 03 Jun 2019 15:37:51: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 15:37:51: #1 finished! INFO @ Mon, 03 Jun 2019 15:37:51: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 15:37:51: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 15:37:51: #2 number of paired peaks: 82 WARNING @ Mon, 03 Jun 2019 15:37:51: Too few paired peaks (82) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 15:37:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX450795/SRX450795.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX450795/SRX450795.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX450795/SRX450795.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX450795/SRX450795.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 15:37:52: #2 number of paired peaks: 82 WARNING @ Mon, 03 Jun 2019 15:37:52: Too few paired peaks (82) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 15:37:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX450795/SRX450795.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX450795/SRX450795.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX450795/SRX450795.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX450795/SRX450795.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 15:37:52: 10000000 INFO @ Mon, 03 Jun 2019 15:37:55: #1 tag size is determined as 41 bps INFO @ Mon, 03 Jun 2019 15:37:55: #1 tag size = 41 INFO @ Mon, 03 Jun 2019 15:37:55: #1 total tags in treatment: 10345527 INFO @ Mon, 03 Jun 2019 15:37:55: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 15:37:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 15:37:55: #1 tags after filtering in treatment: 10345527 INFO @ Mon, 03 Jun 2019 15:37:55: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 15:37:55: #1 finished! INFO @ Mon, 03 Jun 2019 15:37:55: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 15:37:55: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 15:37:56: #2 number of paired peaks: 82 WARNING @ Mon, 03 Jun 2019 15:37:56: Too few paired peaks (82) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 15:37:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX450795/SRX450795.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX450795/SRX450795.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX450795/SRX450795.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX450795/SRX450795.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。