Job ID = 1295769 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 44,778,817 reads read : 44,778,817 reads written : 44,778,817 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:41 44778817 reads; of these: 44778817 (100.00%) were unpaired; of these: 7256627 (16.21%) aligned 0 times 34804803 (77.73%) aligned exactly 1 time 2717387 (6.07%) aligned >1 times 83.79% overall alignment rate Time searching: 00:08:41 Overall time: 00:08:41 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 5050356 / 37522190 = 0.1346 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 15:46:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX450794/SRX450794.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX450794/SRX450794.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX450794/SRX450794.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX450794/SRX450794.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 15:46:13: #1 read tag files... INFO @ Mon, 03 Jun 2019 15:46:13: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 15:46:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX450794/SRX450794.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX450794/SRX450794.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX450794/SRX450794.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX450794/SRX450794.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 15:46:13: #1 read tag files... INFO @ Mon, 03 Jun 2019 15:46:13: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 15:46:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX450794/SRX450794.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX450794/SRX450794.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX450794/SRX450794.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX450794/SRX450794.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 15:46:13: #1 read tag files... INFO @ Mon, 03 Jun 2019 15:46:13: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 15:46:22: 1000000 INFO @ Mon, 03 Jun 2019 15:46:23: 1000000 INFO @ Mon, 03 Jun 2019 15:46:23: 1000000 INFO @ Mon, 03 Jun 2019 15:46:31: 2000000 INFO @ Mon, 03 Jun 2019 15:46:33: 2000000 INFO @ Mon, 03 Jun 2019 15:46:34: 2000000 INFO @ Mon, 03 Jun 2019 15:46:41: 3000000 INFO @ Mon, 03 Jun 2019 15:46:42: 3000000 INFO @ Mon, 03 Jun 2019 15:46:42: 3000000 INFO @ Mon, 03 Jun 2019 15:46:50: 4000000 INFO @ Mon, 03 Jun 2019 15:46:50: 4000000 INFO @ Mon, 03 Jun 2019 15:46:51: 4000000 INFO @ Mon, 03 Jun 2019 15:46:59: 5000000 INFO @ Mon, 03 Jun 2019 15:46:59: 5000000 INFO @ Mon, 03 Jun 2019 15:47:00: 5000000 INFO @ Mon, 03 Jun 2019 15:47:07: 6000000 INFO @ Mon, 03 Jun 2019 15:47:07: 6000000 INFO @ Mon, 03 Jun 2019 15:47:09: 6000000 INFO @ Mon, 03 Jun 2019 15:47:15: 7000000 INFO @ Mon, 03 Jun 2019 15:47:15: 7000000 INFO @ Mon, 03 Jun 2019 15:47:19: 7000000 INFO @ Mon, 03 Jun 2019 15:47:23: 8000000 INFO @ Mon, 03 Jun 2019 15:47:23: 8000000 INFO @ Mon, 03 Jun 2019 15:47:28: 8000000 INFO @ Mon, 03 Jun 2019 15:47:30: 9000000 INFO @ Mon, 03 Jun 2019 15:47:31: 9000000 INFO @ Mon, 03 Jun 2019 15:47:37: 9000000 INFO @ Mon, 03 Jun 2019 15:47:38: 10000000 INFO @ Mon, 03 Jun 2019 15:47:39: 10000000 INFO @ Mon, 03 Jun 2019 15:47:47: 10000000 INFO @ Mon, 03 Jun 2019 15:47:47: 11000000 INFO @ Mon, 03 Jun 2019 15:47:48: 11000000 INFO @ Mon, 03 Jun 2019 15:47:56: 12000000 INFO @ Mon, 03 Jun 2019 15:47:56: 12000000 INFO @ Mon, 03 Jun 2019 15:47:56: 11000000 INFO @ Mon, 03 Jun 2019 15:48:04: 13000000 INFO @ Mon, 03 Jun 2019 15:48:04: 13000000 INFO @ Mon, 03 Jun 2019 15:48:06: 12000000 INFO @ Mon, 03 Jun 2019 15:48:12: 14000000 INFO @ Mon, 03 Jun 2019 15:48:12: 14000000 INFO @ Mon, 03 Jun 2019 15:48:15: 13000000 INFO @ Mon, 03 Jun 2019 15:48:20: 15000000 INFO @ Mon, 03 Jun 2019 15:48:21: 15000000 INFO @ Mon, 03 Jun 2019 15:48:24: 14000000 INFO @ Mon, 03 Jun 2019 15:48:28: 16000000 INFO @ Mon, 03 Jun 2019 15:48:29: 16000000 INFO @ Mon, 03 Jun 2019 15:48:33: 15000000 INFO @ Mon, 03 Jun 2019 15:48:36: 17000000 INFO @ Mon, 03 Jun 2019 15:48:37: 17000000 INFO @ Mon, 03 Jun 2019 15:48:42: 16000000 INFO @ Mon, 03 Jun 2019 15:48:44: 18000000 INFO @ Mon, 03 Jun 2019 15:48:45: 18000000 INFO @ Mon, 03 Jun 2019 15:48:51: 19000000 INFO @ Mon, 03 Jun 2019 15:48:52: 17000000 INFO @ Mon, 03 Jun 2019 15:48:53: 19000000 INFO @ Mon, 03 Jun 2019 15:49:00: 20000000 INFO @ Mon, 03 Jun 2019 15:49:02: 20000000 INFO @ Mon, 03 Jun 2019 15:49:02: 18000000 INFO @ Mon, 03 Jun 2019 15:49:09: 21000000 INFO @ Mon, 03 Jun 2019 15:49:10: 21000000 INFO @ Mon, 03 Jun 2019 15:49:12: 19000000 INFO @ Mon, 03 Jun 2019 15:49:17: 22000000 INFO @ Mon, 03 Jun 2019 15:49:18: 22000000 INFO @ Mon, 03 Jun 2019 15:49:21: 20000000 INFO @ Mon, 03 Jun 2019 15:49:25: 23000000 INFO @ Mon, 03 Jun 2019 15:49:26: 23000000 INFO @ Mon, 03 Jun 2019 15:49:30: 21000000 INFO @ Mon, 03 Jun 2019 15:49:33: 24000000 INFO @ Mon, 03 Jun 2019 15:49:34: 24000000 INFO @ Mon, 03 Jun 2019 15:49:39: 22000000 INFO @ Mon, 03 Jun 2019 15:49:40: 25000000 INFO @ Mon, 03 Jun 2019 15:49:42: 25000000 INFO @ Mon, 03 Jun 2019 15:49:48: 26000000 INFO @ Mon, 03 Jun 2019 15:49:48: 23000000 INFO @ Mon, 03 Jun 2019 15:49:50: 26000000 INFO @ Mon, 03 Jun 2019 15:49:56: 27000000 INFO @ Mon, 03 Jun 2019 15:49:58: 24000000 INFO @ Mon, 03 Jun 2019 15:49:58: 27000000 INFO @ Mon, 03 Jun 2019 15:50:04: 28000000 INFO @ Mon, 03 Jun 2019 15:50:06: 28000000 INFO @ Mon, 03 Jun 2019 15:50:07: 25000000 INFO @ Mon, 03 Jun 2019 15:50:12: 29000000 INFO @ Mon, 03 Jun 2019 15:50:15: 29000000 INFO @ Mon, 03 Jun 2019 15:50:16: 26000000 INFO @ Mon, 03 Jun 2019 15:50:20: 30000000 INFO @ Mon, 03 Jun 2019 15:50:23: 30000000 INFO @ Mon, 03 Jun 2019 15:50:26: 27000000 INFO @ Mon, 03 Jun 2019 15:50:29: 31000000 INFO @ Mon, 03 Jun 2019 15:50:32: 31000000 INFO @ Mon, 03 Jun 2019 15:50:35: 28000000 INFO @ Mon, 03 Jun 2019 15:50:38: 32000000 INFO @ Mon, 03 Jun 2019 15:50:40: 32000000 INFO @ Mon, 03 Jun 2019 15:50:42: #1 tag size is determined as 41 bps INFO @ Mon, 03 Jun 2019 15:50:42: #1 tag size = 41 INFO @ Mon, 03 Jun 2019 15:50:42: #1 total tags in treatment: 32471834 INFO @ Mon, 03 Jun 2019 15:50:42: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 15:50:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 15:50:43: #1 tags after filtering in treatment: 32471834 INFO @ Mon, 03 Jun 2019 15:50:43: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 15:50:43: #1 finished! INFO @ Mon, 03 Jun 2019 15:50:43: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 15:50:43: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 15:50:44: #1 tag size is determined as 41 bps INFO @ Mon, 03 Jun 2019 15:50:44: #1 tag size = 41 INFO @ Mon, 03 Jun 2019 15:50:44: #1 total tags in treatment: 32471834 INFO @ Mon, 03 Jun 2019 15:50:44: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 15:50:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 15:50:45: #1 tags after filtering in treatment: 32471834 INFO @ Mon, 03 Jun 2019 15:50:45: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 15:50:45: #1 finished! INFO @ Mon, 03 Jun 2019 15:50:45: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 15:50:45: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 15:50:45: 29000000 INFO @ Mon, 03 Jun 2019 15:50:45: #2 number of paired peaks: 1 WARNING @ Mon, 03 Jun 2019 15:50:45: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 15:50:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX450794/SRX450794.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX450794/SRX450794.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX450794/SRX450794.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX450794/SRX450794.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 15:50:48: #2 number of paired peaks: 1 WARNING @ Mon, 03 Jun 2019 15:50:48: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 15:50:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX450794/SRX450794.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX450794/SRX450794.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX450794/SRX450794.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX450794/SRX450794.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 15:50:54: 30000000 INFO @ Mon, 03 Jun 2019 15:51:04: 31000000 INFO @ Mon, 03 Jun 2019 15:51:13: 32000000 INFO @ Mon, 03 Jun 2019 15:51:17: #1 tag size is determined as 41 bps INFO @ Mon, 03 Jun 2019 15:51:17: #1 tag size = 41 INFO @ Mon, 03 Jun 2019 15:51:17: #1 total tags in treatment: 32471834 INFO @ Mon, 03 Jun 2019 15:51:17: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 15:51:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 15:51:18: #1 tags after filtering in treatment: 32471834 INFO @ Mon, 03 Jun 2019 15:51:18: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 15:51:18: #1 finished! INFO @ Mon, 03 Jun 2019 15:51:18: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 15:51:18: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 15:51:21: #2 number of paired peaks: 1 WARNING @ Mon, 03 Jun 2019 15:51:21: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 15:51:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX450794/SRX450794.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX450794/SRX450794.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX450794/SRX450794.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX450794/SRX450794.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。