Job ID = 1295761 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 35,703,091 reads read : 35,703,091 reads written : 35,703,091 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:34 35703091 reads; of these: 35703091 (100.00%) were unpaired; of these: 27671731 (77.51%) aligned 0 times 7252900 (20.31%) aligned exactly 1 time 778460 (2.18%) aligned >1 times 22.49% overall alignment rate Time searching: 00:04:34 Overall time: 00:04:34 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 1387491 / 8031360 = 0.1728 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 15:30:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX450791/SRX450791.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX450791/SRX450791.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX450791/SRX450791.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX450791/SRX450791.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 15:30:29: #1 read tag files... INFO @ Mon, 03 Jun 2019 15:30:29: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 15:30:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX450791/SRX450791.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX450791/SRX450791.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX450791/SRX450791.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX450791/SRX450791.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 15:30:29: #1 read tag files... INFO @ Mon, 03 Jun 2019 15:30:29: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 15:30:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX450791/SRX450791.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX450791/SRX450791.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX450791/SRX450791.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX450791/SRX450791.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 15:30:29: #1 read tag files... INFO @ Mon, 03 Jun 2019 15:30:29: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 15:30:37: 1000000 INFO @ Mon, 03 Jun 2019 15:30:39: 1000000 INFO @ Mon, 03 Jun 2019 15:30:39: 1000000 INFO @ Mon, 03 Jun 2019 15:30:45: 2000000 INFO @ Mon, 03 Jun 2019 15:30:48: 2000000 INFO @ Mon, 03 Jun 2019 15:30:49: 2000000 INFO @ Mon, 03 Jun 2019 15:30:52: 3000000 INFO @ Mon, 03 Jun 2019 15:30:57: 3000000 INFO @ Mon, 03 Jun 2019 15:30:59: 3000000 INFO @ Mon, 03 Jun 2019 15:30:59: 4000000 INFO @ Mon, 03 Jun 2019 15:31:06: 4000000 INFO @ Mon, 03 Jun 2019 15:31:07: 5000000 INFO @ Mon, 03 Jun 2019 15:31:08: 4000000 INFO @ Mon, 03 Jun 2019 15:31:15: 6000000 INFO @ Mon, 03 Jun 2019 15:31:15: 5000000 INFO @ Mon, 03 Jun 2019 15:31:18: 5000000 INFO @ Mon, 03 Jun 2019 15:31:20: #1 tag size is determined as 41 bps INFO @ Mon, 03 Jun 2019 15:31:20: #1 tag size = 41 INFO @ Mon, 03 Jun 2019 15:31:20: #1 total tags in treatment: 6643869 INFO @ Mon, 03 Jun 2019 15:31:20: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 15:31:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 15:31:20: #1 tags after filtering in treatment: 6643869 INFO @ Mon, 03 Jun 2019 15:31:20: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 15:31:20: #1 finished! INFO @ Mon, 03 Jun 2019 15:31:20: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 15:31:20: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 15:31:21: #2 number of paired peaks: 34 WARNING @ Mon, 03 Jun 2019 15:31:21: Too few paired peaks (34) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 15:31:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX450791/SRX450791.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX450791/SRX450791.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX450791/SRX450791.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX450791/SRX450791.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 15:31:24: 6000000 INFO @ Mon, 03 Jun 2019 15:31:27: 6000000 INFO @ Mon, 03 Jun 2019 15:31:29: #1 tag size is determined as 41 bps INFO @ Mon, 03 Jun 2019 15:31:29: #1 tag size = 41 INFO @ Mon, 03 Jun 2019 15:31:29: #1 total tags in treatment: 6643869 INFO @ Mon, 03 Jun 2019 15:31:29: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 15:31:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 15:31:30: #1 tags after filtering in treatment: 6643869 INFO @ Mon, 03 Jun 2019 15:31:30: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 15:31:30: #1 finished! INFO @ Mon, 03 Jun 2019 15:31:30: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 15:31:30: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 15:31:30: #2 number of paired peaks: 34 WARNING @ Mon, 03 Jun 2019 15:31:30: Too few paired peaks (34) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 15:31:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX450791/SRX450791.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX450791/SRX450791.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX450791/SRX450791.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX450791/SRX450791.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 15:31:32: #1 tag size is determined as 41 bps INFO @ Mon, 03 Jun 2019 15:31:32: #1 tag size = 41 INFO @ Mon, 03 Jun 2019 15:31:32: #1 total tags in treatment: 6643869 INFO @ Mon, 03 Jun 2019 15:31:32: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 15:31:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 15:31:33: #1 tags after filtering in treatment: 6643869 INFO @ Mon, 03 Jun 2019 15:31:33: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 15:31:33: #1 finished! INFO @ Mon, 03 Jun 2019 15:31:33: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 15:31:33: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 15:31:33: #2 number of paired peaks: 34 WARNING @ Mon, 03 Jun 2019 15:31:33: Too few paired peaks (34) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 15:31:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX450791/SRX450791.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX450791/SRX450791.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX450791/SRX450791.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX450791/SRX450791.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。