Job ID = 11293663 sra ファイルのダウンロード中... Completed: 351182K bytes transferred in 15 seconds (185880K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 11224074 spots for /home/okishinya/chipatlas/results/dm3/SRX4375825/SRR7506479.sra Written 11224074 spots for /home/okishinya/chipatlas/results/dm3/SRX4375825/SRR7506479.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:32 11224074 reads; of these: 11224074 (100.00%) were unpaired; of these: 8801155 (78.41%) aligned 0 times 1962107 (17.48%) aligned exactly 1 time 460812 (4.11%) aligned >1 times 21.59% overall alignment rate Time searching: 00:01:32 Overall time: 00:01:32 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 898985 / 2422919 = 0.3710 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sun, 04 Nov 2018 17:49:39: # Command line: callpeak -t SRX4375825.bam -f BAM -g dm -n SRX4375825.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX4375825.10 # format = BAM # ChIP-seq file = ['SRX4375825.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 04 Nov 2018 17:49:39: # Command line: callpeak -t SRX4375825.bam -f BAM -g dm -n SRX4375825.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX4375825.20 # format = BAM # ChIP-seq file = ['SRX4375825.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 04 Nov 2018 17:49:39: # Command line: callpeak -t SRX4375825.bam -f BAM -g dm -n SRX4375825.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX4375825.05 # format = BAM # ChIP-seq file = ['SRX4375825.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 04 Nov 2018 17:49:39: #1 read tag files... INFO @ Sun, 04 Nov 2018 17:49:39: #1 read tag files... INFO @ Sun, 04 Nov 2018 17:49:39: #1 read tag files... INFO @ Sun, 04 Nov 2018 17:49:39: #1 read treatment tags... INFO @ Sun, 04 Nov 2018 17:49:39: #1 read treatment tags... INFO @ Sun, 04 Nov 2018 17:49:39: #1 read treatment tags... INFO @ Sun, 04 Nov 2018 17:49:46: 1000000 INFO @ Sun, 04 Nov 2018 17:49:47: 1000000 INFO @ Sun, 04 Nov 2018 17:49:47: 1000000 INFO @ Sun, 04 Nov 2018 17:49:50: #1 tag size is determined as 51 bps INFO @ Sun, 04 Nov 2018 17:49:50: #1 tag size = 51 INFO @ Sun, 04 Nov 2018 17:49:50: #1 total tags in treatment: 1523934 INFO @ Sun, 04 Nov 2018 17:49:50: #1 user defined the maximum tags... INFO @ Sun, 04 Nov 2018 17:49:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 04 Nov 2018 17:49:50: #1 tags after filtering in treatment: 1523934 INFO @ Sun, 04 Nov 2018 17:49:50: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 04 Nov 2018 17:49:50: #1 finished! INFO @ Sun, 04 Nov 2018 17:49:50: #2 Build Peak Model... INFO @ Sun, 04 Nov 2018 17:49:50: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 04 Nov 2018 17:49:50: #1 tag size is determined as 51 bps INFO @ Sun, 04 Nov 2018 17:49:50: #1 tag size = 51 INFO @ Sun, 04 Nov 2018 17:49:50: #1 total tags in treatment: 1523934 INFO @ Sun, 04 Nov 2018 17:49:50: #1 user defined the maximum tags... INFO @ Sun, 04 Nov 2018 17:49:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 04 Nov 2018 17:49:50: #1 tag size is determined as 51 bps INFO @ Sun, 04 Nov 2018 17:49:50: #1 tag size = 51 INFO @ Sun, 04 Nov 2018 17:49:50: #1 total tags in treatment: 1523934 INFO @ Sun, 04 Nov 2018 17:49:50: #1 user defined the maximum tags... INFO @ Sun, 04 Nov 2018 17:49:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 04 Nov 2018 17:49:51: #1 tags after filtering in treatment: 1523934 INFO @ Sun, 04 Nov 2018 17:49:51: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 04 Nov 2018 17:49:51: #1 finished! INFO @ Sun, 04 Nov 2018 17:49:51: #2 Build Peak Model... INFO @ Sun, 04 Nov 2018 17:49:51: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 04 Nov 2018 17:49:51: #1 tags after filtering in treatment: 1523934 INFO @ Sun, 04 Nov 2018 17:49:51: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 04 Nov 2018 17:49:51: #1 finished! INFO @ Sun, 04 Nov 2018 17:49:51: #2 Build Peak Model... INFO @ Sun, 04 Nov 2018 17:49:51: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 04 Nov 2018 17:49:51: #2 number of paired peaks: 5515 INFO @ Sun, 04 Nov 2018 17:49:51: start model_add_line... INFO @ Sun, 04 Nov 2018 17:49:51: start X-correlation... INFO @ Sun, 04 Nov 2018 17:49:51: #2 number of paired peaks: 5515 INFO @ Sun, 04 Nov 2018 17:49:51: start model_add_line... INFO @ Sun, 04 Nov 2018 17:49:51: #2 number of paired peaks: 5515 INFO @ Sun, 04 Nov 2018 17:49:51: start model_add_line... INFO @ Sun, 04 Nov 2018 17:49:51: start X-correlation... INFO @ Sun, 04 Nov 2018 17:49:51: start X-correlation... INFO @ Sun, 04 Nov 2018 17:49:51: end of X-cor INFO @ Sun, 04 Nov 2018 17:49:51: end of X-cor INFO @ Sun, 04 Nov 2018 17:49:51: end of X-cor INFO @ Sun, 04 Nov 2018 17:49:51: #2 finished! INFO @ Sun, 04 Nov 2018 17:49:51: #2 finished! INFO @ Sun, 04 Nov 2018 17:49:51: #2 finished! INFO @ Sun, 04 Nov 2018 17:49:51: #2 predicted fragment length is 228 bps INFO @ Sun, 04 Nov 2018 17:49:51: #2 predicted fragment length is 228 bps INFO @ Sun, 04 Nov 2018 17:49:51: #2 predicted fragment length is 228 bps INFO @ Sun, 04 Nov 2018 17:49:51: #2 alternative fragment length(s) may be 228 bps INFO @ Sun, 04 Nov 2018 17:49:51: #2 alternative fragment length(s) may be 228 bps INFO @ Sun, 04 Nov 2018 17:49:51: #2 alternative fragment length(s) may be 228 bps INFO @ Sun, 04 Nov 2018 17:49:51: #2.2 Generate R script for model : SRX4375825.20_model.r INFO @ Sun, 04 Nov 2018 17:49:51: #2.2 Generate R script for model : SRX4375825.10_model.r INFO @ Sun, 04 Nov 2018 17:49:51: #2.2 Generate R script for model : SRX4375825.05_model.r INFO @ Sun, 04 Nov 2018 17:49:51: #3 Call peaks... INFO @ Sun, 04 Nov 2018 17:49:51: #3 Call peaks... INFO @ Sun, 04 Nov 2018 17:49:51: #3 Call peaks... INFO @ Sun, 04 Nov 2018 17:49:51: #3 Pre-compute pvalue-qvalue table... INFO @ Sun, 04 Nov 2018 17:49:51: #3 Pre-compute pvalue-qvalue table... INFO @ Sun, 04 Nov 2018 17:49:51: #3 Pre-compute pvalue-qvalue table... INFO @ Sun, 04 Nov 2018 17:49:56: #3 Call peaks for each chromosome... INFO @ Sun, 04 Nov 2018 17:49:56: #3 Call peaks for each chromosome... INFO @ Sun, 04 Nov 2018 17:49:56: #3 Call peaks for each chromosome... INFO @ Sun, 04 Nov 2018 17:49:58: #4 Write output xls file... SRX4375825.20_peaks.xls INFO @ Sun, 04 Nov 2018 17:49:58: #4 Write peak in narrowPeak format file... SRX4375825.20_peaks.narrowPeak INFO @ Sun, 04 Nov 2018 17:49:58: #4 Write summits bed file... SRX4375825.20_summits.bed INFO @ Sun, 04 Nov 2018 17:49:58: Done! pass1 - making usageList (12 chroms): 1 millis pass2 - checking and writing primary data (2616 records, 4 fields): 6 millis CompletedMACS2peakCalling INFO @ Sun, 04 Nov 2018 17:49:58: #4 Write output xls file... SRX4375825.10_peaks.xls INFO @ Sun, 04 Nov 2018 17:49:58: #4 Write peak in narrowPeak format file... SRX4375825.10_peaks.narrowPeak INFO @ Sun, 04 Nov 2018 17:49:58: #4 Write summits bed file... SRX4375825.10_summits.bed INFO @ Sun, 04 Nov 2018 17:49:58: Done! INFO @ Sun, 04 Nov 2018 17:49:58: #4 Write output xls file... SRX4375825.05_peaks.xls pass1 - making usageList (14 chroms): 1 millis pass2 - checking and writing primary data (3915 records, 4 fields): 8 millis INFO @ Sun, 04 Nov 2018 17:49:59: #4 Write peak in narrowPeak format file... SRX4375825.05_peaks.narrowPeak CompletedMACS2peakCalling INFO @ Sun, 04 Nov 2018 17:49:59: #4 Write summits bed file... SRX4375825.05_summits.bed INFO @ Sun, 04 Nov 2018 17:49:59: Done! pass1 - making usageList (14 chroms): 1 millis pass2 - checking and writing primary data (5244 records, 4 fields): 8 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。