Job ID = 11171381 sra ファイルのダウンロード中... Completed: 137384K bytes transferred in 5 seconds (197078K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 9002283 spots for /home/okishinya/chipatlas/results/dm3/SRX4315053/SRR7444514.sra Written 9002283 spots for /home/okishinya/chipatlas/results/dm3/SRX4315053/SRR7444514.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:03 9002283 reads; of these: 9002283 (100.00%) were unpaired; of these: 1117 (0.01%) aligned 0 times 8081812 (89.78%) aligned exactly 1 time 919354 (10.21%) aligned >1 times 99.99% overall alignment rate Time searching: 00:03:03 Overall time: 00:03:03 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 3517035 / 9001166 = 0.3907 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 08 Sep 2018 14:10:27: # Command line: callpeak -t SRX4315053.bam -f BAM -g dm -n SRX4315053.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX4315053.20 # format = BAM # ChIP-seq file = ['SRX4315053.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Sep 2018 14:10:27: #1 read tag files... INFO @ Sat, 08 Sep 2018 14:10:27: #1 read treatment tags... INFO @ Sat, 08 Sep 2018 14:10:27: # Command line: callpeak -t SRX4315053.bam -f BAM -g dm -n SRX4315053.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX4315053.10 # format = BAM # ChIP-seq file = ['SRX4315053.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Sep 2018 14:10:27: #1 read tag files... INFO @ Sat, 08 Sep 2018 14:10:27: #1 read treatment tags... INFO @ Sat, 08 Sep 2018 14:10:27: # Command line: callpeak -t SRX4315053.bam -f BAM -g dm -n SRX4315053.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX4315053.05 # format = BAM # ChIP-seq file = ['SRX4315053.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Sep 2018 14:10:27: #1 read tag files... INFO @ Sat, 08 Sep 2018 14:10:27: #1 read treatment tags... INFO @ Sat, 08 Sep 2018 14:10:34: 1000000 INFO @ Sat, 08 Sep 2018 14:10:35: 1000000 INFO @ Sat, 08 Sep 2018 14:10:35: 1000000 INFO @ Sat, 08 Sep 2018 14:10:41: 2000000 INFO @ Sat, 08 Sep 2018 14:10:43: 2000000 INFO @ Sat, 08 Sep 2018 14:10:43: 2000000 INFO @ Sat, 08 Sep 2018 14:10:48: 3000000 INFO @ Sat, 08 Sep 2018 14:10:50: 3000000 INFO @ Sat, 08 Sep 2018 14:10:51: 3000000 INFO @ Sat, 08 Sep 2018 14:10:55: 4000000 INFO @ Sat, 08 Sep 2018 14:10:58: 4000000 INFO @ Sat, 08 Sep 2018 14:10:58: 4000000 INFO @ Sat, 08 Sep 2018 14:11:03: 5000000 INFO @ Sat, 08 Sep 2018 14:11:06: 5000000 INFO @ Sat, 08 Sep 2018 14:11:06: #1 tag size is determined as 75 bps INFO @ Sat, 08 Sep 2018 14:11:06: #1 tag size = 75 INFO @ Sat, 08 Sep 2018 14:11:06: #1 total tags in treatment: 5484131 INFO @ Sat, 08 Sep 2018 14:11:06: #1 user defined the maximum tags... INFO @ Sat, 08 Sep 2018 14:11:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Sep 2018 14:11:06: #1 tags after filtering in treatment: 5484131 INFO @ Sat, 08 Sep 2018 14:11:06: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 08 Sep 2018 14:11:06: #1 finished! INFO @ Sat, 08 Sep 2018 14:11:06: #2 Build Peak Model... INFO @ Sat, 08 Sep 2018 14:11:06: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Sep 2018 14:11:06: 5000000 INFO @ Sat, 08 Sep 2018 14:11:07: #2 number of paired peaks: 2727 INFO @ Sat, 08 Sep 2018 14:11:07: start model_add_line... INFO @ Sat, 08 Sep 2018 14:11:07: start X-correlation... INFO @ Sat, 08 Sep 2018 14:11:07: end of X-cor INFO @ Sat, 08 Sep 2018 14:11:07: #2 finished! INFO @ Sat, 08 Sep 2018 14:11:07: #2 predicted fragment length is 103 bps INFO @ Sat, 08 Sep 2018 14:11:07: #2 alternative fragment length(s) may be 103 bps INFO @ Sat, 08 Sep 2018 14:11:07: #2.2 Generate R script for model : SRX4315053.05_model.r WARNING @ Sat, 08 Sep 2018 14:11:07: #2 Since the d (103) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 08 Sep 2018 14:11:07: #2 You may need to consider one of the other alternative d(s): 103 WARNING @ Sat, 08 Sep 2018 14:11:07: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 08 Sep 2018 14:11:07: #3 Call peaks... INFO @ Sat, 08 Sep 2018 14:11:07: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 08 Sep 2018 14:11:10: #1 tag size is determined as 75 bps INFO @ Sat, 08 Sep 2018 14:11:10: #1 tag size = 75 INFO @ Sat, 08 Sep 2018 14:11:10: #1 total tags in treatment: 5484131 INFO @ Sat, 08 Sep 2018 14:11:10: #1 user defined the maximum tags... INFO @ Sat, 08 Sep 2018 14:11:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Sep 2018 14:11:10: #1 tags after filtering in treatment: 5484131 INFO @ Sat, 08 Sep 2018 14:11:10: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 08 Sep 2018 14:11:10: #1 finished! INFO @ Sat, 08 Sep 2018 14:11:10: #2 Build Peak Model... INFO @ Sat, 08 Sep 2018 14:11:10: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Sep 2018 14:11:10: #1 tag size is determined as 75 bps INFO @ Sat, 08 Sep 2018 14:11:10: #1 tag size = 75 INFO @ Sat, 08 Sep 2018 14:11:10: #1 total tags in treatment: 5484131 INFO @ Sat, 08 Sep 2018 14:11:10: #1 user defined the maximum tags... INFO @ Sat, 08 Sep 2018 14:11:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Sep 2018 14:11:10: #1 tags after filtering in treatment: 5484131 INFO @ Sat, 08 Sep 2018 14:11:10: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 08 Sep 2018 14:11:10: #1 finished! INFO @ Sat, 08 Sep 2018 14:11:10: #2 Build Peak Model... INFO @ Sat, 08 Sep 2018 14:11:10: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Sep 2018 14:11:10: #2 number of paired peaks: 2727 INFO @ Sat, 08 Sep 2018 14:11:10: start model_add_line... INFO @ Sat, 08 Sep 2018 14:11:10: start X-correlation... INFO @ Sat, 08 Sep 2018 14:11:10: end of X-cor INFO @ Sat, 08 Sep 2018 14:11:10: #2 finished! INFO @ Sat, 08 Sep 2018 14:11:10: #2 predicted fragment length is 103 bps INFO @ Sat, 08 Sep 2018 14:11:10: #2 alternative fragment length(s) may be 103 bps INFO @ Sat, 08 Sep 2018 14:11:10: #2.2 Generate R script for model : SRX4315053.20_model.r WARNING @ Sat, 08 Sep 2018 14:11:10: #2 Since the d (103) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 08 Sep 2018 14:11:10: #2 You may need to consider one of the other alternative d(s): 103 WARNING @ Sat, 08 Sep 2018 14:11:10: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 08 Sep 2018 14:11:10: #3 Call peaks... INFO @ Sat, 08 Sep 2018 14:11:10: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 08 Sep 2018 14:11:11: #2 number of paired peaks: 2727 INFO @ Sat, 08 Sep 2018 14:11:11: start model_add_line... INFO @ Sat, 08 Sep 2018 14:11:11: start X-correlation... INFO @ Sat, 08 Sep 2018 14:11:11: end of X-cor INFO @ Sat, 08 Sep 2018 14:11:11: #2 finished! INFO @ Sat, 08 Sep 2018 14:11:11: #2 predicted fragment length is 103 bps INFO @ Sat, 08 Sep 2018 14:11:11: #2 alternative fragment length(s) may be 103 bps INFO @ Sat, 08 Sep 2018 14:11:11: #2.2 Generate R script for model : SRX4315053.10_model.r WARNING @ Sat, 08 Sep 2018 14:11:11: #2 Since the d (103) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 08 Sep 2018 14:11:11: #2 You may need to consider one of the other alternative d(s): 103 WARNING @ Sat, 08 Sep 2018 14:11:11: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 08 Sep 2018 14:11:11: #3 Call peaks... INFO @ Sat, 08 Sep 2018 14:11:11: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 08 Sep 2018 14:11:21: #3 Call peaks for each chromosome... INFO @ Sat, 08 Sep 2018 14:11:25: #3 Call peaks for each chromosome... INFO @ Sat, 08 Sep 2018 14:11:26: #3 Call peaks for each chromosome... INFO @ Sat, 08 Sep 2018 14:11:30: #4 Write output xls file... SRX4315053.05_peaks.xls INFO @ Sat, 08 Sep 2018 14:11:30: #4 Write peak in narrowPeak format file... SRX4315053.05_peaks.narrowPeak INFO @ Sat, 08 Sep 2018 14:11:30: #4 Write summits bed file... SRX4315053.05_summits.bed INFO @ Sat, 08 Sep 2018 14:11:30: Done! pass1 - making usageList (14 chroms): 2 millis pass2 - checking and writing primary data (6195 records, 4 fields): 9 millis CompletedMACS2peakCalling INFO @ Sat, 08 Sep 2018 14:11:32: #4 Write output xls file... SRX4315053.10_peaks.xls INFO @ Sat, 08 Sep 2018 14:11:33: #4 Write peak in narrowPeak format file... SRX4315053.10_peaks.narrowPeak INFO @ Sat, 08 Sep 2018 14:11:33: #4 Write summits bed file... SRX4315053.10_summits.bed INFO @ Sat, 08 Sep 2018 14:11:33: Done! pass1 - making usageList (14 chroms): 1 millis pass2 - checking and writing primary data (3351 records, 4 fields): 6 millis CompletedMACS2peakCalling INFO @ Sat, 08 Sep 2018 14:11:33: #4 Write output xls file... SRX4315053.20_peaks.xls INFO @ Sat, 08 Sep 2018 14:11:34: #4 Write peak in narrowPeak format file... SRX4315053.20_peaks.narrowPeak INFO @ Sat, 08 Sep 2018 14:11:34: #4 Write summits bed file... SRX4315053.20_summits.bed INFO @ Sat, 08 Sep 2018 14:11:34: Done! pass1 - making usageList (12 chroms): 1 millis pass2 - checking and writing primary data (1547 records, 4 fields): 4 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。