Job ID = 10714561 sra ファイルのダウンロード中... Completed: 843229K bytes transferred in 10 seconds (628540K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 28276738 spots for /home/okishinya/chipatlas/results/dm3/SRX4047183/SRR7126164.sra Written 28276738 spots for /home/okishinya/chipatlas/results/dm3/SRX4047183/SRR7126164.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:26 28276738 reads; of these: 28276738 (100.00%) were unpaired; of these: 6944932 (24.56%) aligned 0 times 17305017 (61.20%) aligned exactly 1 time 4026789 (14.24%) aligned >1 times 75.44% overall alignment rate Time searching: 00:10:26 Overall time: 00:10:26 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 4181338 / 21331806 = 0.1960 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sun, 03 Jun 2018 13:43:15: # Command line: callpeak -t SRX4047183.bam -f BAM -g dm -n SRX4047183.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX4047183.10 # format = BAM # ChIP-seq file = ['SRX4047183.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 03 Jun 2018 13:43:15: #1 read tag files... INFO @ Sun, 03 Jun 2018 13:43:15: #1 read treatment tags... INFO @ Sun, 03 Jun 2018 13:43:15: # Command line: callpeak -t SRX4047183.bam -f BAM -g dm -n SRX4047183.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX4047183.20 # format = BAM # ChIP-seq file = ['SRX4047183.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 03 Jun 2018 13:43:15: #1 read tag files... INFO @ Sun, 03 Jun 2018 13:43:15: #1 read treatment tags... INFO @ Sun, 03 Jun 2018 13:43:15: # Command line: callpeak -t SRX4047183.bam -f BAM -g dm -n SRX4047183.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX4047183.05 # format = BAM # ChIP-seq file = ['SRX4047183.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 03 Jun 2018 13:43:15: #1 read tag files... INFO @ Sun, 03 Jun 2018 13:43:15: #1 read treatment tags... INFO @ Sun, 03 Jun 2018 13:43:22: 1000000 INFO @ Sun, 03 Jun 2018 13:43:22: 1000000 INFO @ Sun, 03 Jun 2018 13:43:22: 1000000 INFO @ Sun, 03 Jun 2018 13:43:29: 2000000 INFO @ Sun, 03 Jun 2018 13:43:29: 2000000 INFO @ Sun, 03 Jun 2018 13:43:29: 2000000 INFO @ Sun, 03 Jun 2018 13:43:36: 3000000 INFO @ Sun, 03 Jun 2018 13:43:37: 3000000 INFO @ Sun, 03 Jun 2018 13:43:37: 3000000 INFO @ Sun, 03 Jun 2018 13:43:43: 4000000 INFO @ Sun, 03 Jun 2018 13:43:44: 4000000 INFO @ Sun, 03 Jun 2018 13:43:44: 4000000 INFO @ Sun, 03 Jun 2018 13:43:50: 5000000 INFO @ Sun, 03 Jun 2018 13:43:51: 5000000 INFO @ Sun, 03 Jun 2018 13:43:51: 5000000 INFO @ Sun, 03 Jun 2018 13:43:58: 6000000 INFO @ Sun, 03 Jun 2018 13:43:59: 6000000 INFO @ Sun, 03 Jun 2018 13:43:59: 6000000 INFO @ Sun, 03 Jun 2018 13:44:05: 7000000 INFO @ Sun, 03 Jun 2018 13:44:06: 7000000 INFO @ Sun, 03 Jun 2018 13:44:06: 7000000 INFO @ Sun, 03 Jun 2018 13:44:12: 8000000 INFO @ Sun, 03 Jun 2018 13:44:13: 8000000 INFO @ Sun, 03 Jun 2018 13:44:13: 8000000 INFO @ Sun, 03 Jun 2018 13:44:19: 9000000 INFO @ Sun, 03 Jun 2018 13:44:20: 9000000 INFO @ Sun, 03 Jun 2018 13:44:20: 9000000 INFO @ Sun, 03 Jun 2018 13:44:26: 10000000 INFO @ Sun, 03 Jun 2018 13:44:28: 10000000 INFO @ Sun, 03 Jun 2018 13:44:28: 10000000 INFO @ Sun, 03 Jun 2018 13:44:33: 11000000 INFO @ Sun, 03 Jun 2018 13:44:35: 11000000 INFO @ Sun, 03 Jun 2018 13:44:35: 11000000 INFO @ Sun, 03 Jun 2018 13:44:40: 12000000 INFO @ Sun, 03 Jun 2018 13:44:42: 12000000 INFO @ Sun, 03 Jun 2018 13:44:42: 12000000 INFO @ Sun, 03 Jun 2018 13:44:47: 13000000 INFO @ Sun, 03 Jun 2018 13:44:49: 13000000 INFO @ Sun, 03 Jun 2018 13:44:49: 13000000 INFO @ Sun, 03 Jun 2018 13:44:55: 14000000 INFO @ Sun, 03 Jun 2018 13:44:57: 14000000 INFO @ Sun, 03 Jun 2018 13:44:57: 14000000 INFO @ Sun, 03 Jun 2018 13:45:02: 15000000 INFO @ Sun, 03 Jun 2018 13:45:04: 15000000 INFO @ Sun, 03 Jun 2018 13:45:04: 15000000 INFO @ Sun, 03 Jun 2018 13:45:09: 16000000 INFO @ Sun, 03 Jun 2018 13:45:11: 16000000 INFO @ Sun, 03 Jun 2018 13:45:11: 16000000 INFO @ Sun, 03 Jun 2018 13:45:16: 17000000 INFO @ Sun, 03 Jun 2018 13:45:17: #1 tag size is determined as 49 bps INFO @ Sun, 03 Jun 2018 13:45:17: #1 tag size = 49 INFO @ Sun, 03 Jun 2018 13:45:17: #1 total tags in treatment: 17150468 INFO @ Sun, 03 Jun 2018 13:45:17: #1 user defined the maximum tags... INFO @ Sun, 03 Jun 2018 13:45:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 03 Jun 2018 13:45:18: #1 tags after filtering in treatment: 17150468 INFO @ Sun, 03 Jun 2018 13:45:18: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 03 Jun 2018 13:45:18: #1 finished! INFO @ Sun, 03 Jun 2018 13:45:18: #2 Build Peak Model... INFO @ Sun, 03 Jun 2018 13:45:18: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 03 Jun 2018 13:45:19: 17000000 INFO @ Sun, 03 Jun 2018 13:45:19: #2 number of paired peaks: 0 WARNING @ Sun, 03 Jun 2018 13:45:19: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 03 Jun 2018 13:45:19: Process for pairing-model is terminated! cat: SRX4047183.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4047183.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4047183.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4047183.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sun, 03 Jun 2018 13:45:19: 17000000 INFO @ Sun, 03 Jun 2018 13:45:20: #1 tag size is determined as 49 bps INFO @ Sun, 03 Jun 2018 13:45:20: #1 tag size = 49 INFO @ Sun, 03 Jun 2018 13:45:20: #1 total tags in treatment: 17150468 INFO @ Sun, 03 Jun 2018 13:45:20: #1 user defined the maximum tags... INFO @ Sun, 03 Jun 2018 13:45:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 03 Jun 2018 13:45:20: #1 tag size is determined as 49 bps INFO @ Sun, 03 Jun 2018 13:45:20: #1 tag size = 49 INFO @ Sun, 03 Jun 2018 13:45:20: #1 total tags in treatment: 17150468 INFO @ Sun, 03 Jun 2018 13:45:20: #1 user defined the maximum tags... INFO @ Sun, 03 Jun 2018 13:45:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 03 Jun 2018 13:45:20: #1 tags after filtering in treatment: 17150468 INFO @ Sun, 03 Jun 2018 13:45:20: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 03 Jun 2018 13:45:20: #1 finished! INFO @ Sun, 03 Jun 2018 13:45:20: #2 Build Peak Model... INFO @ Sun, 03 Jun 2018 13:45:20: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 03 Jun 2018 13:45:21: #1 tags after filtering in treatment: 17150468 INFO @ Sun, 03 Jun 2018 13:45:21: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 03 Jun 2018 13:45:21: #1 finished! INFO @ Sun, 03 Jun 2018 13:45:21: #2 Build Peak Model... INFO @ Sun, 03 Jun 2018 13:45:21: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 03 Jun 2018 13:45:21: #2 number of paired peaks: 0 WARNING @ Sun, 03 Jun 2018 13:45:21: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 03 Jun 2018 13:45:21: Process for pairing-model is terminated! cat: SRX4047183.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4047183.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4047183.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4047183.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sun, 03 Jun 2018 13:45:22: #2 number of paired peaks: 0 WARNING @ Sun, 03 Jun 2018 13:45:22: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 03 Jun 2018 13:45:22: Process for pairing-model is terminated! cat: SRX4047183.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4047183.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4047183.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4047183.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。