Job ID = 6528073 SRX = SRX3989974 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:11:35 prefetch.2.10.7: 1) Downloading 'SRR7059015'... 2020-06-29T14:11:35 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:12:41 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:12:41 prefetch.2.10.7: 'SRR7059015' is valid 2020-06-29T14:12:41 prefetch.2.10.7: 1) 'SRR7059015' was downloaded successfully 2020-06-29T14:12:41 prefetch.2.10.7: 'SRR7059015' has 0 unresolved dependencies Read 6379144 spots for SRR7059015/SRR7059015.sra Written 6379144 spots for SRR7059015/SRR7059015.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:40 6379144 reads; of these: 6379144 (100.00%) were unpaired; of these: 391667 (6.14%) aligned 0 times 4332070 (67.91%) aligned exactly 1 time 1655407 (25.95%) aligned >1 times 93.86% overall alignment rate Time searching: 00:02:40 Overall time: 00:02:40 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 730520 / 5987477 = 0.1220 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:19:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3989974/SRX3989974.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3989974/SRX3989974.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3989974/SRX3989974.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3989974/SRX3989974.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:19:13: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:19:13: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:19:20: 1000000 INFO @ Mon, 29 Jun 2020 23:19:27: 2000000 INFO @ Mon, 29 Jun 2020 23:19:33: 3000000 INFO @ Mon, 29 Jun 2020 23:19:40: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:19:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3989974/SRX3989974.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3989974/SRX3989974.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3989974/SRX3989974.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3989974/SRX3989974.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:19:43: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:19:43: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:19:46: 5000000 INFO @ Mon, 29 Jun 2020 23:19:48: #1 tag size is determined as 49 bps INFO @ Mon, 29 Jun 2020 23:19:48: #1 tag size = 49 INFO @ Mon, 29 Jun 2020 23:19:48: #1 total tags in treatment: 5256957 INFO @ Mon, 29 Jun 2020 23:19:48: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:19:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:19:48: #1 tags after filtering in treatment: 5256957 INFO @ Mon, 29 Jun 2020 23:19:48: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:19:48: #1 finished! INFO @ Mon, 29 Jun 2020 23:19:48: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:19:48: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:19:49: #2 number of paired peaks: 38 WARNING @ Mon, 29 Jun 2020 23:19:49: Too few paired peaks (38) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:19:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3989974/SRX3989974.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3989974/SRX3989974.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3989974/SRX3989974.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3989974/SRX3989974.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:19:50: 1000000 INFO @ Mon, 29 Jun 2020 23:19:57: 2000000 INFO @ Mon, 29 Jun 2020 23:20:04: 3000000 INFO @ Mon, 29 Jun 2020 23:20:10: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:20:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3989974/SRX3989974.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3989974/SRX3989974.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3989974/SRX3989974.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3989974/SRX3989974.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:20:13: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:20:13: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:20:17: 5000000 INFO @ Mon, 29 Jun 2020 23:20:19: #1 tag size is determined as 49 bps INFO @ Mon, 29 Jun 2020 23:20:19: #1 tag size = 49 INFO @ Mon, 29 Jun 2020 23:20:19: #1 total tags in treatment: 5256957 INFO @ Mon, 29 Jun 2020 23:20:19: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:20:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:20:19: #1 tags after filtering in treatment: 5256957 INFO @ Mon, 29 Jun 2020 23:20:19: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:20:19: #1 finished! INFO @ Mon, 29 Jun 2020 23:20:19: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:20:19: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:20:19: #2 number of paired peaks: 38 WARNING @ Mon, 29 Jun 2020 23:20:19: Too few paired peaks (38) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:20:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3989974/SRX3989974.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3989974/SRX3989974.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3989974/SRX3989974.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3989974/SRX3989974.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:20:20: 1000000 INFO @ Mon, 29 Jun 2020 23:20:27: 2000000 INFO @ Mon, 29 Jun 2020 23:20:34: 3000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 23:20:40: 4000000 INFO @ Mon, 29 Jun 2020 23:20:47: 5000000 INFO @ Mon, 29 Jun 2020 23:20:48: #1 tag size is determined as 49 bps INFO @ Mon, 29 Jun 2020 23:20:48: #1 tag size = 49 INFO @ Mon, 29 Jun 2020 23:20:48: #1 total tags in treatment: 5256957 INFO @ Mon, 29 Jun 2020 23:20:48: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:20:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:20:49: #1 tags after filtering in treatment: 5256957 INFO @ Mon, 29 Jun 2020 23:20:49: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:20:49: #1 finished! INFO @ Mon, 29 Jun 2020 23:20:49: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:20:49: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:20:49: #2 number of paired peaks: 38 WARNING @ Mon, 29 Jun 2020 23:20:49: Too few paired peaks (38) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:20:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3989974/SRX3989974.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3989974/SRX3989974.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3989974/SRX3989974.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3989974/SRX3989974.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。