Job ID = 2590496 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 13,610,938 reads read : 27,221,876 reads written : 13,610,938 reads 0-length : 13,610,938 spots read : 13,774,023 reads read : 27,548,046 reads written : 13,774,023 reads 0-length : 13,774,023 spots read : 13,819,355 reads read : 27,638,710 reads written : 13,819,355 reads 0-length : 13,819,355 fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:15:27 41204316 reads; of these: 41204316 (100.00%) were unpaired; of these: 1140630 (2.77%) aligned 0 times 28874364 (70.08%) aligned exactly 1 time 11189322 (27.16%) aligned >1 times 97.23% overall alignment rate Time searching: 00:15:27 Overall time: 00:15:27 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 4706926 / 40063686 = 0.1175 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 12 Aug 2019 22:39:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981723/SRX3981723.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981723/SRX3981723.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981723/SRX3981723.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981723/SRX3981723.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 22:39:55: #1 read tag files... INFO @ Mon, 12 Aug 2019 22:39:55: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 22:39:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981723/SRX3981723.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981723/SRX3981723.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981723/SRX3981723.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981723/SRX3981723.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 22:39:56: #1 read tag files... INFO @ Mon, 12 Aug 2019 22:39:56: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 22:39:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981723/SRX3981723.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981723/SRX3981723.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981723/SRX3981723.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981723/SRX3981723.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 22:39:57: #1 read tag files... INFO @ Mon, 12 Aug 2019 22:39:57: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 22:40:02: 1000000 INFO @ Mon, 12 Aug 2019 22:40:05: 1000000 INFO @ Mon, 12 Aug 2019 22:40:06: 1000000 INFO @ Mon, 12 Aug 2019 22:40:09: 2000000 INFO @ Mon, 12 Aug 2019 22:40:13: 2000000 INFO @ Mon, 12 Aug 2019 22:40:15: 2000000 INFO @ Mon, 12 Aug 2019 22:40:17: 3000000 INFO @ Mon, 12 Aug 2019 22:40:21: 3000000 INFO @ Mon, 12 Aug 2019 22:40:24: 4000000 INFO @ Mon, 12 Aug 2019 22:40:24: 3000000 INFO @ Mon, 12 Aug 2019 22:40:28: 4000000 INFO @ Mon, 12 Aug 2019 22:40:31: 5000000 INFO @ Mon, 12 Aug 2019 22:40:33: 4000000 INFO @ Mon, 12 Aug 2019 22:40:36: 5000000 INFO @ Mon, 12 Aug 2019 22:40:38: 6000000 INFO @ Mon, 12 Aug 2019 22:40:42: 5000000 INFO @ Mon, 12 Aug 2019 22:40:43: 6000000 INFO @ Mon, 12 Aug 2019 22:40:45: 7000000 INFO @ Mon, 12 Aug 2019 22:40:50: 6000000 INFO @ Mon, 12 Aug 2019 22:40:51: 7000000 INFO @ Mon, 12 Aug 2019 22:40:52: 8000000 INFO @ Mon, 12 Aug 2019 22:40:58: 8000000 INFO @ Mon, 12 Aug 2019 22:40:59: 9000000 INFO @ Mon, 12 Aug 2019 22:40:59: 7000000 INFO @ Mon, 12 Aug 2019 22:41:06: 9000000 INFO @ Mon, 12 Aug 2019 22:41:06: 10000000 INFO @ Mon, 12 Aug 2019 22:41:08: 8000000 INFO @ Mon, 12 Aug 2019 22:41:13: 11000000 INFO @ Mon, 12 Aug 2019 22:41:13: 10000000 INFO @ Mon, 12 Aug 2019 22:41:17: 9000000 INFO @ Mon, 12 Aug 2019 22:41:20: 12000000 INFO @ Mon, 12 Aug 2019 22:41:21: 11000000 INFO @ Mon, 12 Aug 2019 22:41:26: 10000000 INFO @ Mon, 12 Aug 2019 22:41:28: 13000000 INFO @ Mon, 12 Aug 2019 22:41:29: 12000000 INFO @ Mon, 12 Aug 2019 22:41:35: 11000000 INFO @ Mon, 12 Aug 2019 22:41:36: 14000000 INFO @ Mon, 12 Aug 2019 22:41:37: 13000000 INFO @ Mon, 12 Aug 2019 22:41:43: 12000000 INFO @ Mon, 12 Aug 2019 22:41:44: 15000000 INFO @ Mon, 12 Aug 2019 22:41:45: 14000000 INFO @ Mon, 12 Aug 2019 22:41:52: 13000000 INFO @ Mon, 12 Aug 2019 22:41:53: 16000000 INFO @ Mon, 12 Aug 2019 22:41:54: 15000000 INFO @ Mon, 12 Aug 2019 22:42:01: 17000000 INFO @ Mon, 12 Aug 2019 22:42:02: 14000000 INFO @ Mon, 12 Aug 2019 22:42:02: 16000000 INFO @ Mon, 12 Aug 2019 22:42:09: 18000000 INFO @ Mon, 12 Aug 2019 22:42:10: 17000000 INFO @ Mon, 12 Aug 2019 22:42:11: 15000000 INFO @ Mon, 12 Aug 2019 22:42:18: 19000000 INFO @ Mon, 12 Aug 2019 22:42:18: 18000000 INFO @ Mon, 12 Aug 2019 22:42:20: 16000000 INFO @ Mon, 12 Aug 2019 22:42:26: 19000000 INFO @ Mon, 12 Aug 2019 22:42:28: 20000000 INFO @ Mon, 12 Aug 2019 22:42:29: 17000000 INFO @ Mon, 12 Aug 2019 22:42:35: 20000000 INFO @ Mon, 12 Aug 2019 22:42:37: 21000000 INFO @ Mon, 12 Aug 2019 22:42:37: 18000000 INFO @ Mon, 12 Aug 2019 22:42:43: 21000000 INFO @ Mon, 12 Aug 2019 22:42:47: 19000000 INFO @ Mon, 12 Aug 2019 22:42:47: 22000000 INFO @ Mon, 12 Aug 2019 22:42:51: 22000000 INFO @ Mon, 12 Aug 2019 22:42:56: 20000000 INFO @ Mon, 12 Aug 2019 22:42:57: 23000000 INFO @ Mon, 12 Aug 2019 22:42:59: 23000000 INFO @ Mon, 12 Aug 2019 22:43:05: 21000000 INFO @ Mon, 12 Aug 2019 22:43:06: 24000000 INFO @ Mon, 12 Aug 2019 22:43:08: 24000000 INFO @ Mon, 12 Aug 2019 22:43:14: 22000000 INFO @ Mon, 12 Aug 2019 22:43:16: 25000000 INFO @ Mon, 12 Aug 2019 22:43:16: 25000000 INFO @ Mon, 12 Aug 2019 22:43:23: 23000000 INFO @ Mon, 12 Aug 2019 22:43:24: 26000000 INFO @ Mon, 12 Aug 2019 22:43:24: 26000000 INFO @ Mon, 12 Aug 2019 22:43:31: 24000000 INFO @ Mon, 12 Aug 2019 22:43:33: 27000000 INFO @ Mon, 12 Aug 2019 22:43:33: 27000000 INFO @ Mon, 12 Aug 2019 22:43:41: 28000000 INFO @ Mon, 12 Aug 2019 22:43:41: 25000000 INFO @ Mon, 12 Aug 2019 22:43:41: 28000000 INFO @ Mon, 12 Aug 2019 22:43:49: 29000000 INFO @ Mon, 12 Aug 2019 22:43:49: 29000000 INFO @ Mon, 12 Aug 2019 22:43:50: 26000000 INFO @ Mon, 12 Aug 2019 22:43:57: 30000000 INFO @ Mon, 12 Aug 2019 22:43:57: 30000000 INFO @ Mon, 12 Aug 2019 22:43:59: 27000000 INFO @ Mon, 12 Aug 2019 22:44:05: 31000000 INFO @ Mon, 12 Aug 2019 22:44:05: 31000000 INFO @ Mon, 12 Aug 2019 22:44:08: 28000000 INFO @ Mon, 12 Aug 2019 22:44:13: 32000000 INFO @ Mon, 12 Aug 2019 22:44:13: 32000000 INFO @ Mon, 12 Aug 2019 22:44:17: 29000000 INFO @ Mon, 12 Aug 2019 22:44:21: 33000000 INFO @ Mon, 12 Aug 2019 22:44:21: 33000000 INFO @ Mon, 12 Aug 2019 22:44:25: 30000000 INFO @ Mon, 12 Aug 2019 22:44:29: 34000000 INFO @ Mon, 12 Aug 2019 22:44:29: 34000000 INFO @ Mon, 12 Aug 2019 22:44:34: 31000000 INFO @ Mon, 12 Aug 2019 22:44:38: 35000000 INFO @ Mon, 12 Aug 2019 22:44:38: 35000000 INFO @ Mon, 12 Aug 2019 22:44:41: #1 tag size is determined as 51 bps INFO @ Mon, 12 Aug 2019 22:44:41: #1 tag size = 51 INFO @ Mon, 12 Aug 2019 22:44:41: #1 total tags in treatment: 35356760 INFO @ Mon, 12 Aug 2019 22:44:41: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 22:44:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 22:44:41: #1 tag size is determined as 51 bps INFO @ Mon, 12 Aug 2019 22:44:41: #1 tag size = 51 INFO @ Mon, 12 Aug 2019 22:44:41: #1 total tags in treatment: 35356760 INFO @ Mon, 12 Aug 2019 22:44:41: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 22:44:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 22:44:41: #1 tags after filtering in treatment: 35356760 INFO @ Mon, 12 Aug 2019 22:44:41: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 22:44:41: #1 finished! INFO @ Mon, 12 Aug 2019 22:44:41: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 22:44:41: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 22:44:42: #1 tags after filtering in treatment: 35356760 INFO @ Mon, 12 Aug 2019 22:44:42: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 22:44:42: #1 finished! INFO @ Mon, 12 Aug 2019 22:44:42: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 22:44:42: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 22:44:43: 32000000 INFO @ Mon, 12 Aug 2019 22:44:44: #2 number of paired peaks: 11 WARNING @ Mon, 12 Aug 2019 22:44:44: Too few paired peaks (11) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 22:44:44: Process for pairing-model is terminated! INFO @ Mon, 12 Aug 2019 22:44:44: #2 number of paired peaks: 11 WARNING @ Mon, 12 Aug 2019 22:44:44: Too few paired peaks (11) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 22:44:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981723/SRX3981723.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981723/SRX3981723.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981723/SRX3981723.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981723/SRX3981723.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling cut: /home/okishinya/chipatlas/results/dm3/SRX3981723/SRX3981723.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981723/SRX3981723.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981723/SRX3981723.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981723/SRX3981723.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 22:44:51: 33000000 INFO @ Mon, 12 Aug 2019 22:44:59: 34000000 INFO @ Mon, 12 Aug 2019 22:45:07: 35000000 INFO @ Mon, 12 Aug 2019 22:45:10: #1 tag size is determined as 51 bps INFO @ Mon, 12 Aug 2019 22:45:10: #1 tag size = 51 INFO @ Mon, 12 Aug 2019 22:45:10: #1 total tags in treatment: 35356760 INFO @ Mon, 12 Aug 2019 22:45:10: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 22:45:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 22:45:11: #1 tags after filtering in treatment: 35356760 INFO @ Mon, 12 Aug 2019 22:45:11: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 22:45:11: #1 finished! INFO @ Mon, 12 Aug 2019 22:45:11: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 22:45:11: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 22:45:14: #2 number of paired peaks: 11 WARNING @ Mon, 12 Aug 2019 22:45:14: Too few paired peaks (11) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 22:45:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981723/SRX3981723.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981723/SRX3981723.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981723/SRX3981723.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981723/SRX3981723.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。