Job ID = 2590495 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 13,346,840 reads read : 26,693,680 reads written : 13,346,840 reads 0-length : 13,346,840 spots read : 13,499,766 reads read : 26,999,532 reads written : 13,499,766 reads 0-length : 13,499,766 spots read : 13,550,595 reads read : 27,101,190 reads written : 13,550,595 reads 0-length : 13,550,595 fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:16:17 40397201 reads; of these: 40397201 (100.00%) were unpaired; of these: 1108219 (2.74%) aligned 0 times 28258918 (69.95%) aligned exactly 1 time 11030064 (27.30%) aligned >1 times 97.26% overall alignment rate Time searching: 00:16:17 Overall time: 00:16:17 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 4523925 / 39288982 = 0.1151 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 12 Aug 2019 22:38:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981722/SRX3981722.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981722/SRX3981722.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981722/SRX3981722.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981722/SRX3981722.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 22:38:59: #1 read tag files... INFO @ Mon, 12 Aug 2019 22:38:59: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 22:39:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981722/SRX3981722.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981722/SRX3981722.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981722/SRX3981722.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981722/SRX3981722.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 22:39:00: #1 read tag files... INFO @ Mon, 12 Aug 2019 22:39:00: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 22:39:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981722/SRX3981722.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981722/SRX3981722.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981722/SRX3981722.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981722/SRX3981722.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 22:39:01: #1 read tag files... INFO @ Mon, 12 Aug 2019 22:39:01: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 22:39:08: 1000000 INFO @ Mon, 12 Aug 2019 22:39:08: 1000000 INFO @ Mon, 12 Aug 2019 22:39:09: 1000000 INFO @ Mon, 12 Aug 2019 22:39:15: 2000000 INFO @ Mon, 12 Aug 2019 22:39:16: 2000000 INFO @ Mon, 12 Aug 2019 22:39:18: 2000000 INFO @ Mon, 12 Aug 2019 22:39:23: 3000000 INFO @ Mon, 12 Aug 2019 22:39:24: 3000000 INFO @ Mon, 12 Aug 2019 22:39:27: 3000000 INFO @ Mon, 12 Aug 2019 22:39:30: 4000000 INFO @ Mon, 12 Aug 2019 22:39:32: 4000000 INFO @ Mon, 12 Aug 2019 22:39:36: 4000000 INFO @ Mon, 12 Aug 2019 22:39:37: 5000000 INFO @ Mon, 12 Aug 2019 22:39:40: 5000000 INFO @ Mon, 12 Aug 2019 22:39:44: 6000000 INFO @ Mon, 12 Aug 2019 22:39:45: 5000000 INFO @ Mon, 12 Aug 2019 22:39:48: 6000000 INFO @ Mon, 12 Aug 2019 22:39:51: 7000000 INFO @ Mon, 12 Aug 2019 22:39:56: 6000000 INFO @ Mon, 12 Aug 2019 22:39:58: 8000000 INFO @ Mon, 12 Aug 2019 22:40:00: 7000000 INFO @ Mon, 12 Aug 2019 22:40:05: 9000000 INFO @ Mon, 12 Aug 2019 22:40:05: 7000000 INFO @ Mon, 12 Aug 2019 22:40:08: 8000000 INFO @ Mon, 12 Aug 2019 22:40:12: 10000000 INFO @ Mon, 12 Aug 2019 22:40:14: 8000000 INFO @ Mon, 12 Aug 2019 22:40:16: 9000000 INFO @ Mon, 12 Aug 2019 22:40:19: 11000000 INFO @ Mon, 12 Aug 2019 22:40:23: 9000000 INFO @ Mon, 12 Aug 2019 22:40:24: 10000000 INFO @ Mon, 12 Aug 2019 22:40:26: 12000000 INFO @ Mon, 12 Aug 2019 22:40:32: 11000000 INFO @ Mon, 12 Aug 2019 22:40:32: 10000000 INFO @ Mon, 12 Aug 2019 22:40:33: 13000000 INFO @ Mon, 12 Aug 2019 22:40:39: 12000000 INFO @ Mon, 12 Aug 2019 22:40:40: 14000000 INFO @ Mon, 12 Aug 2019 22:40:41: 11000000 INFO @ Mon, 12 Aug 2019 22:40:47: 13000000 INFO @ Mon, 12 Aug 2019 22:40:47: 15000000 INFO @ Mon, 12 Aug 2019 22:40:50: 12000000 INFO @ Mon, 12 Aug 2019 22:40:55: 16000000 INFO @ Mon, 12 Aug 2019 22:40:55: 14000000 INFO @ Mon, 12 Aug 2019 22:40:59: 13000000 INFO @ Mon, 12 Aug 2019 22:41:02: 17000000 INFO @ Mon, 12 Aug 2019 22:41:03: 15000000 INFO @ Mon, 12 Aug 2019 22:41:08: 14000000 INFO @ Mon, 12 Aug 2019 22:41:09: 18000000 INFO @ Mon, 12 Aug 2019 22:41:11: 16000000 INFO @ Mon, 12 Aug 2019 22:41:16: 19000000 INFO @ Mon, 12 Aug 2019 22:41:17: 15000000 INFO @ Mon, 12 Aug 2019 22:41:18: 17000000 INFO @ Mon, 12 Aug 2019 22:41:23: 20000000 INFO @ Mon, 12 Aug 2019 22:41:26: 18000000 INFO @ Mon, 12 Aug 2019 22:41:26: 16000000 INFO @ Mon, 12 Aug 2019 22:41:30: 21000000 INFO @ Mon, 12 Aug 2019 22:41:34: 19000000 INFO @ Mon, 12 Aug 2019 22:41:35: 17000000 INFO @ Mon, 12 Aug 2019 22:41:37: 22000000 INFO @ Mon, 12 Aug 2019 22:41:42: 20000000 INFO @ Mon, 12 Aug 2019 22:41:44: 23000000 INFO @ Mon, 12 Aug 2019 22:41:44: 18000000 INFO @ Mon, 12 Aug 2019 22:41:50: 21000000 INFO @ Mon, 12 Aug 2019 22:41:51: 24000000 INFO @ Mon, 12 Aug 2019 22:41:53: 19000000 INFO @ Mon, 12 Aug 2019 22:41:57: 22000000 INFO @ Mon, 12 Aug 2019 22:41:58: 25000000 INFO @ Mon, 12 Aug 2019 22:42:02: 20000000 INFO @ Mon, 12 Aug 2019 22:42:05: 23000000 INFO @ Mon, 12 Aug 2019 22:42:05: 26000000 INFO @ Mon, 12 Aug 2019 22:42:11: 21000000 INFO @ Mon, 12 Aug 2019 22:42:12: 27000000 INFO @ Mon, 12 Aug 2019 22:42:13: 24000000 INFO @ Mon, 12 Aug 2019 22:42:19: 28000000 INFO @ Mon, 12 Aug 2019 22:42:20: 22000000 INFO @ Mon, 12 Aug 2019 22:42:21: 25000000 INFO @ Mon, 12 Aug 2019 22:42:26: 29000000 INFO @ Mon, 12 Aug 2019 22:42:29: 26000000 INFO @ Mon, 12 Aug 2019 22:42:29: 23000000 INFO @ Mon, 12 Aug 2019 22:42:34: 30000000 INFO @ Mon, 12 Aug 2019 22:42:36: 27000000 INFO @ Mon, 12 Aug 2019 22:42:38: 24000000 INFO @ Mon, 12 Aug 2019 22:42:41: 31000000 INFO @ Mon, 12 Aug 2019 22:42:44: 28000000 INFO @ Mon, 12 Aug 2019 22:42:47: 25000000 INFO @ Mon, 12 Aug 2019 22:42:48: 32000000 INFO @ Mon, 12 Aug 2019 22:42:52: 29000000 INFO @ Mon, 12 Aug 2019 22:42:55: 33000000 INFO @ Mon, 12 Aug 2019 22:42:56: 26000000 INFO @ Mon, 12 Aug 2019 22:43:00: 30000000 INFO @ Mon, 12 Aug 2019 22:43:02: 34000000 INFO @ Mon, 12 Aug 2019 22:43:05: 27000000 INFO @ Mon, 12 Aug 2019 22:43:08: #1 tag size is determined as 51 bps INFO @ Mon, 12 Aug 2019 22:43:08: #1 tag size = 51 INFO @ Mon, 12 Aug 2019 22:43:08: #1 total tags in treatment: 34765057 INFO @ Mon, 12 Aug 2019 22:43:08: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 22:43:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 22:43:08: 31000000 INFO @ Mon, 12 Aug 2019 22:43:08: #1 tags after filtering in treatment: 34765057 INFO @ Mon, 12 Aug 2019 22:43:08: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 22:43:08: #1 finished! INFO @ Mon, 12 Aug 2019 22:43:08: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 22:43:08: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 22:43:11: #2 number of paired peaks: 15 WARNING @ Mon, 12 Aug 2019 22:43:11: Too few paired peaks (15) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 22:43:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981722/SRX3981722.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981722/SRX3981722.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981722/SRX3981722.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981722/SRX3981722.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 22:43:14: 28000000 INFO @ Mon, 12 Aug 2019 22:43:15: 32000000 INFO @ Mon, 12 Aug 2019 22:43:23: 33000000 INFO @ Mon, 12 Aug 2019 22:43:23: 29000000 INFO @ Mon, 12 Aug 2019 22:43:31: 34000000 INFO @ Mon, 12 Aug 2019 22:43:32: 30000000 INFO @ Mon, 12 Aug 2019 22:43:37: #1 tag size is determined as 51 bps INFO @ Mon, 12 Aug 2019 22:43:37: #1 tag size = 51 INFO @ Mon, 12 Aug 2019 22:43:37: #1 total tags in treatment: 34765057 INFO @ Mon, 12 Aug 2019 22:43:37: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 22:43:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 22:43:38: #1 tags after filtering in treatment: 34765057 INFO @ Mon, 12 Aug 2019 22:43:38: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 22:43:38: #1 finished! INFO @ Mon, 12 Aug 2019 22:43:38: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 22:43:38: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 22:43:41: #2 number of paired peaks: 15 WARNING @ Mon, 12 Aug 2019 22:43:41: Too few paired peaks (15) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 22:43:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981722/SRX3981722.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981722/SRX3981722.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981722/SRX3981722.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981722/SRX3981722.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 22:43:41: 31000000 INFO @ Mon, 12 Aug 2019 22:43:50: 32000000 INFO @ Mon, 12 Aug 2019 22:43:59: 33000000 INFO @ Mon, 12 Aug 2019 22:44:08: 34000000 INFO @ Mon, 12 Aug 2019 22:44:15: #1 tag size is determined as 51 bps INFO @ Mon, 12 Aug 2019 22:44:15: #1 tag size = 51 INFO @ Mon, 12 Aug 2019 22:44:15: #1 total tags in treatment: 34765057 INFO @ Mon, 12 Aug 2019 22:44:15: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 22:44:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 22:44:15: #1 tags after filtering in treatment: 34765057 INFO @ Mon, 12 Aug 2019 22:44:15: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 22:44:15: #1 finished! INFO @ Mon, 12 Aug 2019 22:44:15: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 22:44:15: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 22:44:18: #2 number of paired peaks: 15 WARNING @ Mon, 12 Aug 2019 22:44:18: Too few paired peaks (15) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 22:44:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981722/SRX3981722.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981722/SRX3981722.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981722/SRX3981722.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981722/SRX3981722.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。