Job ID = 6528068 SRX = SRX3981720 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:16:05 prefetch.2.10.7: 1) Downloading 'SRR7050572'... 2020-06-29T14:16:05 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:17:05 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:17:06 prefetch.2.10.7: 'SRR7050572' is valid 2020-06-29T14:17:06 prefetch.2.10.7: 1) 'SRR7050572' was downloaded successfully 2020-06-29T14:17:06 prefetch.2.10.7: 'SRR7050572' has 0 unresolved dependencies Read 12786427 spots for SRR7050572/SRR7050572.sra Written 12786427 spots for SRR7050572/SRR7050572.sra 2020-06-29T14:17:58 prefetch.2.10.7: 1) Downloading 'SRR7050573'... 2020-06-29T14:17:58 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:18:51 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:18:52 prefetch.2.10.7: 'SRR7050573' is valid 2020-06-29T14:18:52 prefetch.2.10.7: 1) 'SRR7050573' was downloaded successfully 2020-06-29T14:18:52 prefetch.2.10.7: 'SRR7050573' has 0 unresolved dependencies Read 12941440 spots for SRR7050573/SRR7050573.sra Written 12941440 spots for SRR7050573/SRR7050573.sra 2020-06-29T14:19:44 prefetch.2.10.7: 1) Downloading 'SRR7050574'... 2020-06-29T14:19:44 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:20:46 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:20:46 prefetch.2.10.7: 'SRR7050574' is valid 2020-06-29T14:20:46 prefetch.2.10.7: 1) 'SRR7050574' was downloaded successfully 2020-06-29T14:20:46 prefetch.2.10.7: 'SRR7050574' has 0 unresolved dependencies Read 13071550 spots for SRR7050574/SRR7050574.sra Written 13071550 spots for SRR7050574/SRR7050574.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:12:25 38799417 reads; of these: 38799417 (100.00%) were unpaired; of these: 1082501 (2.79%) aligned 0 times 27042235 (69.70%) aligned exactly 1 time 10674681 (27.51%) aligned >1 times 97.21% overall alignment rate Time searching: 00:12:26 Overall time: 00:12:26 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 4280819 / 37716916 = 0.1135 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:50:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981720/SRX3981720.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981720/SRX3981720.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981720/SRX3981720.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981720/SRX3981720.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:50:26: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:50:26: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:50:31: 1000000 INFO @ Mon, 29 Jun 2020 23:50:37: 2000000 INFO @ Mon, 29 Jun 2020 23:50:42: 3000000 INFO @ Mon, 29 Jun 2020 23:50:47: 4000000 INFO @ Mon, 29 Jun 2020 23:50:53: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:50:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981720/SRX3981720.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981720/SRX3981720.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981720/SRX3981720.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981720/SRX3981720.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:50:56: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:50:56: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:50:58: 6000000 INFO @ Mon, 29 Jun 2020 23:51:03: 1000000 INFO @ Mon, 29 Jun 2020 23:51:05: 7000000 INFO @ Mon, 29 Jun 2020 23:51:10: 2000000 INFO @ Mon, 29 Jun 2020 23:51:11: 8000000 INFO @ Mon, 29 Jun 2020 23:51:17: 3000000 INFO @ Mon, 29 Jun 2020 23:51:18: 9000000 INFO @ Mon, 29 Jun 2020 23:51:23: 4000000 INFO @ Mon, 29 Jun 2020 23:51:24: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:51:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981720/SRX3981720.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981720/SRX3981720.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981720/SRX3981720.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981720/SRX3981720.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:51:26: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:51:26: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:51:30: 5000000 INFO @ Mon, 29 Jun 2020 23:51:31: 11000000 INFO @ Mon, 29 Jun 2020 23:51:32: 1000000 INFO @ Mon, 29 Jun 2020 23:51:36: 6000000 INFO @ Mon, 29 Jun 2020 23:51:37: 12000000 INFO @ Mon, 29 Jun 2020 23:51:39: 2000000 INFO @ Mon, 29 Jun 2020 23:51:42: 7000000 INFO @ Mon, 29 Jun 2020 23:51:43: 13000000 INFO @ Mon, 29 Jun 2020 23:51:45: 3000000 INFO @ Mon, 29 Jun 2020 23:51:49: 8000000 INFO @ Mon, 29 Jun 2020 23:51:50: 14000000 INFO @ Mon, 29 Jun 2020 23:51:52: 4000000 INFO @ Mon, 29 Jun 2020 23:51:55: 9000000 INFO @ Mon, 29 Jun 2020 23:51:56: 15000000 INFO @ Mon, 29 Jun 2020 23:51:58: 5000000 INFO @ Mon, 29 Jun 2020 23:52:02: 10000000 INFO @ Mon, 29 Jun 2020 23:52:02: 16000000 INFO @ Mon, 29 Jun 2020 23:52:05: 6000000 INFO @ Mon, 29 Jun 2020 23:52:08: 11000000 INFO @ Mon, 29 Jun 2020 23:52:09: 17000000 INFO @ Mon, 29 Jun 2020 23:52:11: 7000000 INFO @ Mon, 29 Jun 2020 23:52:14: 12000000 INFO @ Mon, 29 Jun 2020 23:52:15: 18000000 INFO @ Mon, 29 Jun 2020 23:52:17: 8000000 INFO @ Mon, 29 Jun 2020 23:52:21: 13000000 INFO @ Mon, 29 Jun 2020 23:52:21: 19000000 INFO @ Mon, 29 Jun 2020 23:52:24: 9000000 INFO @ Mon, 29 Jun 2020 23:52:27: 14000000 INFO @ Mon, 29 Jun 2020 23:52:28: 20000000 INFO @ Mon, 29 Jun 2020 23:52:30: 10000000 INFO @ Mon, 29 Jun 2020 23:52:34: 15000000 INFO @ Mon, 29 Jun 2020 23:52:34: 21000000 INFO @ Mon, 29 Jun 2020 23:52:36: 11000000 INFO @ Mon, 29 Jun 2020 23:52:40: 16000000 INFO @ Mon, 29 Jun 2020 23:52:40: 22000000 INFO @ Mon, 29 Jun 2020 23:52:43: 12000000 INFO @ Mon, 29 Jun 2020 23:52:46: 17000000 INFO @ Mon, 29 Jun 2020 23:52:47: 23000000 INFO @ Mon, 29 Jun 2020 23:52:49: 13000000 INFO @ Mon, 29 Jun 2020 23:52:52: 18000000 INFO @ Mon, 29 Jun 2020 23:52:53: 24000000 INFO @ Mon, 29 Jun 2020 23:52:55: 14000000 INFO @ Mon, 29 Jun 2020 23:52:59: 19000000 INFO @ Mon, 29 Jun 2020 23:52:59: 25000000 INFO @ Mon, 29 Jun 2020 23:53:01: 15000000 INFO @ Mon, 29 Jun 2020 23:53:05: 20000000 INFO @ Mon, 29 Jun 2020 23:53:05: 26000000 INFO @ Mon, 29 Jun 2020 23:53:08: 16000000 INFO @ Mon, 29 Jun 2020 23:53:11: 21000000 INFO @ Mon, 29 Jun 2020 23:53:12: 27000000 INFO @ Mon, 29 Jun 2020 23:53:14: 17000000 INFO @ Mon, 29 Jun 2020 23:53:18: 22000000 INFO @ Mon, 29 Jun 2020 23:53:18: 28000000 INFO @ Mon, 29 Jun 2020 23:53:20: 18000000 INFO @ Mon, 29 Jun 2020 23:53:24: 23000000 INFO @ Mon, 29 Jun 2020 23:53:24: 29000000 INFO @ Mon, 29 Jun 2020 23:53:26: 19000000 INFO @ Mon, 29 Jun 2020 23:53:30: 24000000 INFO @ Mon, 29 Jun 2020 23:53:31: 30000000 INFO @ Mon, 29 Jun 2020 23:53:33: 20000000 INFO @ Mon, 29 Jun 2020 23:53:36: 25000000 INFO @ Mon, 29 Jun 2020 23:53:37: 31000000 INFO @ Mon, 29 Jun 2020 23:53:39: 21000000 INFO @ Mon, 29 Jun 2020 23:53:43: 26000000 INFO @ Mon, 29 Jun 2020 23:53:43: 32000000 INFO @ Mon, 29 Jun 2020 23:53:45: 22000000 INFO @ Mon, 29 Jun 2020 23:53:49: 27000000 INFO @ Mon, 29 Jun 2020 23:53:49: 33000000 INFO @ Mon, 29 Jun 2020 23:53:51: 23000000 INFO @ Mon, 29 Jun 2020 23:53:52: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:53:52: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:53:52: #1 total tags in treatment: 33436097 INFO @ Mon, 29 Jun 2020 23:53:52: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:53:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:53:53: #1 tags after filtering in treatment: 33436097 INFO @ Mon, 29 Jun 2020 23:53:53: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:53:53: #1 finished! INFO @ Mon, 29 Jun 2020 23:53:53: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:53:53: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:53:55: #2 number of paired peaks: 26 WARNING @ Mon, 29 Jun 2020 23:53:55: Too few paired peaks (26) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:53:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981720/SRX3981720.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981720/SRX3981720.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981720/SRX3981720.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981720/SRX3981720.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:53:55: 28000000 INFO @ Mon, 29 Jun 2020 23:53:58: 24000000 INFO @ Mon, 29 Jun 2020 23:54:01: 29000000 INFO @ Mon, 29 Jun 2020 23:54:04: 25000000 INFO @ Mon, 29 Jun 2020 23:54:08: 30000000 INFO @ Mon, 29 Jun 2020 23:54:10: 26000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 23:54:14: 31000000 INFO @ Mon, 29 Jun 2020 23:54:17: 27000000 INFO @ Mon, 29 Jun 2020 23:54:21: 32000000 INFO @ Mon, 29 Jun 2020 23:54:23: 28000000 INFO @ Mon, 29 Jun 2020 23:54:27: 33000000 INFO @ Mon, 29 Jun 2020 23:54:30: 29000000 INFO @ Mon, 29 Jun 2020 23:54:30: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:54:30: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:54:30: #1 total tags in treatment: 33436097 INFO @ Mon, 29 Jun 2020 23:54:30: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:54:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:54:30: #1 tags after filtering in treatment: 33436097 INFO @ Mon, 29 Jun 2020 23:54:30: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:54:30: #1 finished! INFO @ Mon, 29 Jun 2020 23:54:30: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:54:30: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:54:32: #2 number of paired peaks: 26 WARNING @ Mon, 29 Jun 2020 23:54:32: Too few paired peaks (26) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:54:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981720/SRX3981720.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981720/SRX3981720.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981720/SRX3981720.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981720/SRX3981720.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:54:36: 30000000 INFO @ Mon, 29 Jun 2020 23:54:42: 31000000 INFO @ Mon, 29 Jun 2020 23:54:48: 32000000 INFO @ Mon, 29 Jun 2020 23:54:54: 33000000 INFO @ Mon, 29 Jun 2020 23:54:57: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:54:57: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:54:57: #1 total tags in treatment: 33436097 INFO @ Mon, 29 Jun 2020 23:54:57: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:54:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:54:57: #1 tags after filtering in treatment: 33436097 INFO @ Mon, 29 Jun 2020 23:54:57: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:54:57: #1 finished! INFO @ Mon, 29 Jun 2020 23:54:57: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:54:57: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:54:59: #2 number of paired peaks: 26 WARNING @ Mon, 29 Jun 2020 23:54:59: Too few paired peaks (26) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:54:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981720/SRX3981720.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981720/SRX3981720.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981720/SRX3981720.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981720/SRX3981720.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。