Job ID = 2590471 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 9,601,109 reads read : 19,202,218 reads written : 9,601,109 reads 0-length : 9,601,109 spots read : 9,596,163 reads read : 19,192,326 reads written : 9,596,163 reads 0-length : 9,596,163 spots read : 9,632,729 reads read : 19,265,458 reads written : 9,632,729 reads 0-length : 9,632,729 spots read : 9,675,584 reads read : 19,351,168 reads written : 9,675,584 reads 0-length : 9,675,584 fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:13:07 38505585 reads; of these: 38505585 (100.00%) were unpaired; of these: 1140754 (2.96%) aligned 0 times 28808362 (74.82%) aligned exactly 1 time 8556469 (22.22%) aligned >1 times 97.04% overall alignment rate Time searching: 00:13:07 Overall time: 00:13:07 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 4661410 / 37364831 = 0.1248 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 12 Aug 2019 22:24:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981702/SRX3981702.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981702/SRX3981702.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981702/SRX3981702.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981702/SRX3981702.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 22:24:04: #1 read tag files... INFO @ Mon, 12 Aug 2019 22:24:04: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 22:24:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981702/SRX3981702.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981702/SRX3981702.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981702/SRX3981702.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981702/SRX3981702.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 22:24:05: #1 read tag files... INFO @ Mon, 12 Aug 2019 22:24:05: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 22:24:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981702/SRX3981702.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981702/SRX3981702.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981702/SRX3981702.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981702/SRX3981702.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 22:24:06: #1 read tag files... INFO @ Mon, 12 Aug 2019 22:24:06: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 22:24:12: 1000000 INFO @ Mon, 12 Aug 2019 22:24:14: 1000000 INFO @ Mon, 12 Aug 2019 22:24:16: 1000000 INFO @ Mon, 12 Aug 2019 22:24:20: 2000000 INFO @ Mon, 12 Aug 2019 22:24:23: 2000000 INFO @ Mon, 12 Aug 2019 22:24:25: 2000000 INFO @ Mon, 12 Aug 2019 22:24:27: 3000000 INFO @ Mon, 12 Aug 2019 22:24:32: 3000000 INFO @ Mon, 12 Aug 2019 22:24:34: 4000000 INFO @ Mon, 12 Aug 2019 22:24:35: 3000000 INFO @ Mon, 12 Aug 2019 22:24:41: 5000000 INFO @ Mon, 12 Aug 2019 22:24:41: 4000000 INFO @ Mon, 12 Aug 2019 22:24:44: 4000000 INFO @ Mon, 12 Aug 2019 22:24:48: 6000000 INFO @ Mon, 12 Aug 2019 22:24:50: 5000000 INFO @ Mon, 12 Aug 2019 22:24:53: 5000000 INFO @ Mon, 12 Aug 2019 22:24:55: 7000000 INFO @ Mon, 12 Aug 2019 22:24:59: 6000000 INFO @ Mon, 12 Aug 2019 22:25:02: 8000000 INFO @ Mon, 12 Aug 2019 22:25:02: 6000000 INFO @ Mon, 12 Aug 2019 22:25:07: 7000000 INFO @ Mon, 12 Aug 2019 22:25:09: 9000000 INFO @ Mon, 12 Aug 2019 22:25:11: 7000000 INFO @ Mon, 12 Aug 2019 22:25:16: 10000000 INFO @ Mon, 12 Aug 2019 22:25:16: 8000000 INFO @ Mon, 12 Aug 2019 22:25:21: 8000000 INFO @ Mon, 12 Aug 2019 22:25:23: 11000000 INFO @ Mon, 12 Aug 2019 22:25:25: 9000000 INFO @ Mon, 12 Aug 2019 22:25:30: 9000000 INFO @ Mon, 12 Aug 2019 22:25:31: 12000000 INFO @ Mon, 12 Aug 2019 22:25:34: 10000000 INFO @ Mon, 12 Aug 2019 22:25:38: 13000000 INFO @ Mon, 12 Aug 2019 22:25:39: 10000000 INFO @ Mon, 12 Aug 2019 22:25:43: 11000000 INFO @ Mon, 12 Aug 2019 22:25:45: 14000000 INFO @ Mon, 12 Aug 2019 22:25:49: 11000000 INFO @ Mon, 12 Aug 2019 22:25:51: 12000000 INFO @ Mon, 12 Aug 2019 22:25:53: 15000000 INFO @ Mon, 12 Aug 2019 22:25:58: 12000000 INFO @ Mon, 12 Aug 2019 22:26:00: 16000000 INFO @ Mon, 12 Aug 2019 22:26:00: 13000000 INFO @ Mon, 12 Aug 2019 22:26:07: 13000000 INFO @ Mon, 12 Aug 2019 22:26:07: 17000000 INFO @ Mon, 12 Aug 2019 22:26:09: 14000000 INFO @ Mon, 12 Aug 2019 22:26:14: 18000000 INFO @ Mon, 12 Aug 2019 22:26:16: 14000000 INFO @ Mon, 12 Aug 2019 22:26:18: 15000000 INFO @ Mon, 12 Aug 2019 22:26:21: 19000000 INFO @ Mon, 12 Aug 2019 22:26:25: 15000000 INFO @ Mon, 12 Aug 2019 22:26:26: 16000000 INFO @ Mon, 12 Aug 2019 22:26:28: 20000000 INFO @ Mon, 12 Aug 2019 22:26:34: 16000000 INFO @ Mon, 12 Aug 2019 22:26:35: 17000000 INFO @ Mon, 12 Aug 2019 22:26:35: 21000000 INFO @ Mon, 12 Aug 2019 22:26:42: 22000000 INFO @ Mon, 12 Aug 2019 22:26:43: 17000000 INFO @ Mon, 12 Aug 2019 22:26:44: 18000000 INFO @ Mon, 12 Aug 2019 22:26:49: 23000000 INFO @ Mon, 12 Aug 2019 22:26:52: 18000000 INFO @ Mon, 12 Aug 2019 22:26:52: 19000000 INFO @ Mon, 12 Aug 2019 22:26:56: 24000000 INFO @ Mon, 12 Aug 2019 22:27:01: 20000000 INFO @ Mon, 12 Aug 2019 22:27:01: 19000000 INFO @ Mon, 12 Aug 2019 22:27:03: 25000000 INFO @ Mon, 12 Aug 2019 22:27:10: 21000000 INFO @ Mon, 12 Aug 2019 22:27:10: 26000000 INFO @ Mon, 12 Aug 2019 22:27:11: 20000000 INFO @ Mon, 12 Aug 2019 22:27:17: 27000000 INFO @ Mon, 12 Aug 2019 22:27:18: 22000000 INFO @ Mon, 12 Aug 2019 22:27:20: 21000000 INFO @ Mon, 12 Aug 2019 22:27:25: 28000000 INFO @ Mon, 12 Aug 2019 22:27:27: 23000000 INFO @ Mon, 12 Aug 2019 22:27:29: 22000000 INFO @ Mon, 12 Aug 2019 22:27:32: 29000000 INFO @ Mon, 12 Aug 2019 22:27:36: 24000000 INFO @ Mon, 12 Aug 2019 22:27:38: 23000000 INFO @ Mon, 12 Aug 2019 22:27:39: 30000000 INFO @ Mon, 12 Aug 2019 22:27:45: 25000000 INFO @ Mon, 12 Aug 2019 22:27:47: 31000000 INFO @ Mon, 12 Aug 2019 22:27:47: 24000000 INFO @ Mon, 12 Aug 2019 22:27:53: 26000000 INFO @ Mon, 12 Aug 2019 22:27:54: 32000000 INFO @ Mon, 12 Aug 2019 22:27:56: 25000000 INFO @ Mon, 12 Aug 2019 22:27:59: #1 tag size is determined as 51 bps INFO @ Mon, 12 Aug 2019 22:27:59: #1 tag size = 51 INFO @ Mon, 12 Aug 2019 22:27:59: #1 total tags in treatment: 32703421 INFO @ Mon, 12 Aug 2019 22:27:59: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 22:27:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 22:28:00: #1 tags after filtering in treatment: 32703421 INFO @ Mon, 12 Aug 2019 22:28:00: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 22:28:00: #1 finished! INFO @ Mon, 12 Aug 2019 22:28:00: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 22:28:00: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 22:28:02: 27000000 INFO @ Mon, 12 Aug 2019 22:28:03: #2 number of paired peaks: 55 WARNING @ Mon, 12 Aug 2019 22:28:03: Too few paired peaks (55) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 22:28:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981702/SRX3981702.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981702/SRX3981702.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981702/SRX3981702.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981702/SRX3981702.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 22:28:05: 26000000 INFO @ Mon, 12 Aug 2019 22:28:11: 28000000 INFO @ Mon, 12 Aug 2019 22:28:14: 27000000 INFO @ Mon, 12 Aug 2019 22:28:20: 29000000 INFO @ Mon, 12 Aug 2019 22:28:24: 28000000 INFO @ Mon, 12 Aug 2019 22:28:28: 30000000 INFO @ Mon, 12 Aug 2019 22:28:33: 29000000 INFO @ Mon, 12 Aug 2019 22:28:37: 31000000 INFO @ Mon, 12 Aug 2019 22:28:42: 30000000 INFO @ Mon, 12 Aug 2019 22:28:46: 32000000 INFO @ Mon, 12 Aug 2019 22:28:51: 31000000 INFO @ Mon, 12 Aug 2019 22:28:52: #1 tag size is determined as 51 bps INFO @ Mon, 12 Aug 2019 22:28:52: #1 tag size = 51 INFO @ Mon, 12 Aug 2019 22:28:52: #1 total tags in treatment: 32703421 INFO @ Mon, 12 Aug 2019 22:28:52: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 22:28:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 22:28:53: #1 tags after filtering in treatment: 32703421 INFO @ Mon, 12 Aug 2019 22:28:53: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 22:28:53: #1 finished! INFO @ Mon, 12 Aug 2019 22:28:53: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 22:28:53: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 22:28:55: #2 number of paired peaks: 55 WARNING @ Mon, 12 Aug 2019 22:28:55: Too few paired peaks (55) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 22:28:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981702/SRX3981702.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981702/SRX3981702.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981702/SRX3981702.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981702/SRX3981702.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 22:29:00: 32000000 INFO @ Mon, 12 Aug 2019 22:29:06: #1 tag size is determined as 51 bps INFO @ Mon, 12 Aug 2019 22:29:06: #1 tag size = 51 INFO @ Mon, 12 Aug 2019 22:29:06: #1 total tags in treatment: 32703421 INFO @ Mon, 12 Aug 2019 22:29:06: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 22:29:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 22:29:07: #1 tags after filtering in treatment: 32703421 INFO @ Mon, 12 Aug 2019 22:29:07: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 22:29:07: #1 finished! INFO @ Mon, 12 Aug 2019 22:29:07: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 22:29:07: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 22:29:10: #2 number of paired peaks: 55 WARNING @ Mon, 12 Aug 2019 22:29:10: Too few paired peaks (55) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 22:29:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981702/SRX3981702.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981702/SRX3981702.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981702/SRX3981702.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981702/SRX3981702.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。