Job ID = 6528063 SRX = SRX3981699 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:46:13 prefetch.2.10.7: 1) Downloading 'SRR7050491'... 2020-06-29T14:46:13 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:46:59 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:46:59 prefetch.2.10.7: 'SRR7050491' is valid 2020-06-29T14:46:59 prefetch.2.10.7: 1) 'SRR7050491' was downloaded successfully 2020-06-29T14:46:59 prefetch.2.10.7: 'SRR7050491' has 0 unresolved dependencies Read 9426413 spots for SRR7050491/SRR7050491.sra Written 9426413 spots for SRR7050491/SRR7050491.sra 2020-06-29T14:47:49 prefetch.2.10.7: 1) Downloading 'SRR7050492'... 2020-06-29T14:47:49 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:48:40 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:48:40 prefetch.2.10.7: 'SRR7050492' is valid 2020-06-29T14:48:40 prefetch.2.10.7: 1) 'SRR7050492' was downloaded successfully 2020-06-29T14:48:40 prefetch.2.10.7: 'SRR7050492' has 0 unresolved dependencies Read 9407230 spots for SRR7050492/SRR7050492.sra Written 9407230 spots for SRR7050492/SRR7050492.sra 2020-06-29T14:49:30 prefetch.2.10.7: 1) Downloading 'SRR7050493'... 2020-06-29T14:49:30 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:50:12 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:50:13 prefetch.2.10.7: 'SRR7050493' is valid 2020-06-29T14:50:13 prefetch.2.10.7: 1) 'SRR7050493' was downloaded successfully 2020-06-29T14:50:13 prefetch.2.10.7: 'SRR7050493' has 0 unresolved dependencies Read 9445954 spots for SRR7050493/SRR7050493.sra Written 9445954 spots for SRR7050493/SRR7050493.sra 2020-06-29T14:51:02 prefetch.2.10.7: 1) Downloading 'SRR7050494'... 2020-06-29T14:51:02 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:51:59 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:51:59 prefetch.2.10.7: 'SRR7050494' is valid 2020-06-29T14:51:59 prefetch.2.10.7: 1) 'SRR7050494' was downloaded successfully 2020-06-29T14:51:59 prefetch.2.10.7: 'SRR7050494' has 0 unresolved dependencies Read 9493908 spots for SRR7050494/SRR7050494.sra Written 9493908 spots for SRR7050494/SRR7050494.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:12:25 37773505 reads; of these: 37773505 (100.00%) were unpaired; of these: 1334203 (3.53%) aligned 0 times 27299159 (72.27%) aligned exactly 1 time 9140143 (24.20%) aligned >1 times 96.47% overall alignment rate Time searching: 00:12:25 Overall time: 00:12:25 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 4496062 / 36439302 = 0.1234 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:24:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981699/SRX3981699.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981699/SRX3981699.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981699/SRX3981699.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981699/SRX3981699.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:24:47: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:24:47: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:24:52: 1000000 INFO @ Tue, 30 Jun 2020 00:24:57: 2000000 INFO @ Tue, 30 Jun 2020 00:25:02: 3000000 INFO @ Tue, 30 Jun 2020 00:25:07: 4000000 INFO @ Tue, 30 Jun 2020 00:25:12: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:25:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981699/SRX3981699.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981699/SRX3981699.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981699/SRX3981699.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981699/SRX3981699.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:25:16: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:25:16: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:25:16: 6000000 INFO @ Tue, 30 Jun 2020 00:25:22: 7000000 INFO @ Tue, 30 Jun 2020 00:25:22: 1000000 INFO @ Tue, 30 Jun 2020 00:25:27: 8000000 INFO @ Tue, 30 Jun 2020 00:25:28: 2000000 INFO @ Tue, 30 Jun 2020 00:25:33: 9000000 INFO @ Tue, 30 Jun 2020 00:25:33: 3000000 INFO @ Tue, 30 Jun 2020 00:25:38: 10000000 INFO @ Tue, 30 Jun 2020 00:25:38: 4000000 INFO @ Tue, 30 Jun 2020 00:25:43: 11000000 INFO @ Tue, 30 Jun 2020 00:25:44: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:25:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981699/SRX3981699.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981699/SRX3981699.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981699/SRX3981699.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981699/SRX3981699.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:25:46: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:25:46: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:25:49: 12000000 INFO @ Tue, 30 Jun 2020 00:25:49: 6000000 INFO @ Tue, 30 Jun 2020 00:25:52: 1000000 INFO @ Tue, 30 Jun 2020 00:25:55: 13000000 INFO @ Tue, 30 Jun 2020 00:25:55: 7000000 INFO @ Tue, 30 Jun 2020 00:25:58: 2000000 INFO @ Tue, 30 Jun 2020 00:26:01: 14000000 INFO @ Tue, 30 Jun 2020 00:26:01: 8000000 INFO @ Tue, 30 Jun 2020 00:26:04: 3000000 INFO @ Tue, 30 Jun 2020 00:26:06: 15000000 INFO @ Tue, 30 Jun 2020 00:26:07: 9000000 INFO @ Tue, 30 Jun 2020 00:26:10: 4000000 INFO @ Tue, 30 Jun 2020 00:26:12: 16000000 INFO @ Tue, 30 Jun 2020 00:26:13: 10000000 INFO @ Tue, 30 Jun 2020 00:26:16: 5000000 INFO @ Tue, 30 Jun 2020 00:26:18: 17000000 INFO @ Tue, 30 Jun 2020 00:26:18: 11000000 INFO @ Tue, 30 Jun 2020 00:26:21: 6000000 INFO @ Tue, 30 Jun 2020 00:26:24: 18000000 INFO @ Tue, 30 Jun 2020 00:26:24: 12000000 INFO @ Tue, 30 Jun 2020 00:26:27: 7000000 INFO @ Tue, 30 Jun 2020 00:26:29: 19000000 INFO @ Tue, 30 Jun 2020 00:26:30: 13000000 INFO @ Tue, 30 Jun 2020 00:26:33: 8000000 INFO @ Tue, 30 Jun 2020 00:26:35: 20000000 INFO @ Tue, 30 Jun 2020 00:26:36: 14000000 INFO @ Tue, 30 Jun 2020 00:26:39: 9000000 INFO @ Tue, 30 Jun 2020 00:26:41: 21000000 INFO @ Tue, 30 Jun 2020 00:26:42: 15000000 INFO @ Tue, 30 Jun 2020 00:26:45: 10000000 INFO @ Tue, 30 Jun 2020 00:26:47: 22000000 INFO @ Tue, 30 Jun 2020 00:26:48: 16000000 INFO @ Tue, 30 Jun 2020 00:26:50: 11000000 INFO @ Tue, 30 Jun 2020 00:26:53: 23000000 INFO @ Tue, 30 Jun 2020 00:26:54: 17000000 INFO @ Tue, 30 Jun 2020 00:26:56: 12000000 INFO @ Tue, 30 Jun 2020 00:26:58: 24000000 INFO @ Tue, 30 Jun 2020 00:26:59: 18000000 INFO @ Tue, 30 Jun 2020 00:27:02: 13000000 INFO @ Tue, 30 Jun 2020 00:27:04: 25000000 INFO @ Tue, 30 Jun 2020 00:27:05: 19000000 INFO @ Tue, 30 Jun 2020 00:27:08: 14000000 INFO @ Tue, 30 Jun 2020 00:27:10: 26000000 INFO @ Tue, 30 Jun 2020 00:27:11: 20000000 INFO @ Tue, 30 Jun 2020 00:27:14: 15000000 INFO @ Tue, 30 Jun 2020 00:27:16: 27000000 INFO @ Tue, 30 Jun 2020 00:27:17: 21000000 INFO @ Tue, 30 Jun 2020 00:27:20: 16000000 INFO @ Tue, 30 Jun 2020 00:27:21: 28000000 INFO @ Tue, 30 Jun 2020 00:27:23: 22000000 INFO @ Tue, 30 Jun 2020 00:27:26: 17000000 INFO @ Tue, 30 Jun 2020 00:27:27: 29000000 INFO @ Tue, 30 Jun 2020 00:27:29: 23000000 INFO @ Tue, 30 Jun 2020 00:27:31: 18000000 INFO @ Tue, 30 Jun 2020 00:27:33: 30000000 INFO @ Tue, 30 Jun 2020 00:27:34: 24000000 INFO @ Tue, 30 Jun 2020 00:27:37: 19000000 INFO @ Tue, 30 Jun 2020 00:27:39: 31000000 INFO @ Tue, 30 Jun 2020 00:27:40: 25000000 INFO @ Tue, 30 Jun 2020 00:27:43: 20000000 INFO @ Tue, 30 Jun 2020 00:27:44: #1 tag size is determined as 51 bps INFO @ Tue, 30 Jun 2020 00:27:44: #1 tag size = 51 INFO @ Tue, 30 Jun 2020 00:27:44: #1 total tags in treatment: 31943240 INFO @ Tue, 30 Jun 2020 00:27:44: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:27:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:27:45: #1 tags after filtering in treatment: 31943240 INFO @ Tue, 30 Jun 2020 00:27:45: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:27:45: #1 finished! INFO @ Tue, 30 Jun 2020 00:27:45: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:27:45: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:27:46: 26000000 INFO @ Tue, 30 Jun 2020 00:27:47: #2 number of paired peaks: 45 WARNING @ Tue, 30 Jun 2020 00:27:47: Too few paired peaks (45) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:27:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981699/SRX3981699.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981699/SRX3981699.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981699/SRX3981699.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981699/SRX3981699.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:27:48: 21000000 INFO @ Tue, 30 Jun 2020 00:27:51: 27000000 INFO @ Tue, 30 Jun 2020 00:27:54: 22000000 INFO @ Tue, 30 Jun 2020 00:27:57: 28000000 INFO @ Tue, 30 Jun 2020 00:27:59: 23000000 INFO @ Tue, 30 Jun 2020 00:28:02: 29000000 INFO @ Tue, 30 Jun 2020 00:28:05: 24000000 INFO @ Tue, 30 Jun 2020 00:28:08: 30000000 INFO @ Tue, 30 Jun 2020 00:28:10: 25000000 INFO @ Tue, 30 Jun 2020 00:28:13: 31000000 INFO @ Tue, 30 Jun 2020 00:28:15: 26000000 INFO @ Tue, 30 Jun 2020 00:28:19: #1 tag size is determined as 51 bps INFO @ Tue, 30 Jun 2020 00:28:19: #1 tag size = 51 INFO @ Tue, 30 Jun 2020 00:28:19: #1 total tags in treatment: 31943240 INFO @ Tue, 30 Jun 2020 00:28:19: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:28:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:28:19: #1 tags after filtering in treatment: 31943240 INFO @ Tue, 30 Jun 2020 00:28:19: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:28:19: #1 finished! INFO @ Tue, 30 Jun 2020 00:28:19: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:28:19: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:28:21: 27000000 INFO @ Tue, 30 Jun 2020 00:28:21: #2 number of paired peaks: 45 WARNING @ Tue, 30 Jun 2020 00:28:21: Too few paired peaks (45) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:28:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981699/SRX3981699.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981699/SRX3981699.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981699/SRX3981699.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981699/SRX3981699.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:28:25: 28000000 INFO @ Tue, 30 Jun 2020 00:28:30: 29000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 00:28:35: 30000000 INFO @ Tue, 30 Jun 2020 00:28:40: 31000000 INFO @ Tue, 30 Jun 2020 00:28:45: #1 tag size is determined as 51 bps INFO @ Tue, 30 Jun 2020 00:28:45: #1 tag size = 51 INFO @ Tue, 30 Jun 2020 00:28:45: #1 total tags in treatment: 31943240 INFO @ Tue, 30 Jun 2020 00:28:45: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:28:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:28:45: #1 tags after filtering in treatment: 31943240 INFO @ Tue, 30 Jun 2020 00:28:45: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:28:45: #1 finished! INFO @ Tue, 30 Jun 2020 00:28:45: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:28:45: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:28:48: #2 number of paired peaks: 45 WARNING @ Tue, 30 Jun 2020 00:28:48: Too few paired peaks (45) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:28:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981699/SRX3981699.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981699/SRX3981699.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981699/SRX3981699.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981699/SRX3981699.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。