Job ID = 6528058 SRX = SRX3981678 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:22:36 prefetch.2.10.7: 1) Downloading 'SRR7050418'... 2020-06-29T14:22:36 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:23:56 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:23:56 prefetch.2.10.7: 'SRR7050418' is valid 2020-06-29T14:23:56 prefetch.2.10.7: 1) 'SRR7050418' was downloaded successfully 2020-06-29T14:23:56 prefetch.2.10.7: 'SRR7050418' has 0 unresolved dependencies Read 13054686 spots for SRR7050418/SRR7050418.sra Written 13054686 spots for SRR7050418/SRR7050418.sra 2020-06-29T14:24:52 prefetch.2.10.7: 1) Downloading 'SRR7050419'... 2020-06-29T14:24:52 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:26:20 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:26:21 prefetch.2.10.7: 'SRR7050419' is valid 2020-06-29T14:26:21 prefetch.2.10.7: 1) 'SRR7050419' was downloaded successfully 2020-06-29T14:26:21 prefetch.2.10.7: 'SRR7050419' has 0 unresolved dependencies Read 13069624 spots for SRR7050419/SRR7050419.sra Written 13069624 spots for SRR7050419/SRR7050419.sra 2020-06-29T14:27:17 prefetch.2.10.7: 1) Downloading 'SRR7050420'... 2020-06-29T14:27:17 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:28:59 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:29:00 prefetch.2.10.7: 'SRR7050420' is valid 2020-06-29T14:29:00 prefetch.2.10.7: 1) 'SRR7050420' was downloaded successfully 2020-06-29T14:29:00 prefetch.2.10.7: 'SRR7050420' has 0 unresolved dependencies Read 12988020 spots for SRR7050420/SRR7050420.sra Written 12988020 spots for SRR7050420/SRR7050420.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:12:19 39112330 reads; of these: 39112330 (100.00%) were unpaired; of these: 1214337 (3.10%) aligned 0 times 27685070 (70.78%) aligned exactly 1 time 10212923 (26.11%) aligned >1 times 96.90% overall alignment rate Time searching: 00:12:19 Overall time: 00:12:19 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 4304108 / 37897993 = 0.1136 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:59:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981678/SRX3981678.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981678/SRX3981678.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981678/SRX3981678.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981678/SRX3981678.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:59:49: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:59:49: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:59:56: 1000000 INFO @ Tue, 30 Jun 2020 00:00:03: 2000000 INFO @ Tue, 30 Jun 2020 00:00:10: 3000000 INFO @ Tue, 30 Jun 2020 00:00:17: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:00:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981678/SRX3981678.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981678/SRX3981678.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981678/SRX3981678.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981678/SRX3981678.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:00:19: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:00:19: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:00:24: 5000000 INFO @ Tue, 30 Jun 2020 00:00:27: 1000000 INFO @ Tue, 30 Jun 2020 00:00:31: 6000000 INFO @ Tue, 30 Jun 2020 00:00:34: 2000000 INFO @ Tue, 30 Jun 2020 00:00:38: 7000000 INFO @ Tue, 30 Jun 2020 00:00:41: 3000000 INFO @ Tue, 30 Jun 2020 00:00:45: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:00:48: 4000000 INFO @ Tue, 30 Jun 2020 00:00:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981678/SRX3981678.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981678/SRX3981678.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981678/SRX3981678.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981678/SRX3981678.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:00:49: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:00:49: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:00:52: 9000000 INFO @ Tue, 30 Jun 2020 00:00:55: 5000000 INFO @ Tue, 30 Jun 2020 00:00:56: 1000000 INFO @ Tue, 30 Jun 2020 00:00:59: 10000000 INFO @ Tue, 30 Jun 2020 00:01:02: 6000000 INFO @ Tue, 30 Jun 2020 00:01:02: 2000000 INFO @ Tue, 30 Jun 2020 00:01:06: 11000000 INFO @ Tue, 30 Jun 2020 00:01:08: 3000000 INFO @ Tue, 30 Jun 2020 00:01:09: 7000000 INFO @ Tue, 30 Jun 2020 00:01:13: 12000000 INFO @ Tue, 30 Jun 2020 00:01:15: 4000000 INFO @ Tue, 30 Jun 2020 00:01:16: 8000000 INFO @ Tue, 30 Jun 2020 00:01:20: 13000000 INFO @ Tue, 30 Jun 2020 00:01:21: 5000000 INFO @ Tue, 30 Jun 2020 00:01:23: 9000000 INFO @ Tue, 30 Jun 2020 00:01:27: 14000000 INFO @ Tue, 30 Jun 2020 00:01:27: 6000000 INFO @ Tue, 30 Jun 2020 00:01:31: 10000000 INFO @ Tue, 30 Jun 2020 00:01:33: 15000000 INFO @ Tue, 30 Jun 2020 00:01:33: 7000000 INFO @ Tue, 30 Jun 2020 00:01:38: 11000000 INFO @ Tue, 30 Jun 2020 00:01:40: 8000000 INFO @ Tue, 30 Jun 2020 00:01:40: 16000000 INFO @ Tue, 30 Jun 2020 00:01:45: 12000000 INFO @ Tue, 30 Jun 2020 00:01:46: 9000000 INFO @ Tue, 30 Jun 2020 00:01:47: 17000000 INFO @ Tue, 30 Jun 2020 00:01:52: 13000000 INFO @ Tue, 30 Jun 2020 00:01:52: 10000000 INFO @ Tue, 30 Jun 2020 00:01:54: 18000000 INFO @ Tue, 30 Jun 2020 00:01:59: 11000000 INFO @ Tue, 30 Jun 2020 00:01:59: 14000000 INFO @ Tue, 30 Jun 2020 00:02:01: 19000000 INFO @ Tue, 30 Jun 2020 00:02:05: 12000000 INFO @ Tue, 30 Jun 2020 00:02:06: 15000000 INFO @ Tue, 30 Jun 2020 00:02:08: 20000000 INFO @ Tue, 30 Jun 2020 00:02:11: 13000000 INFO @ Tue, 30 Jun 2020 00:02:13: 16000000 INFO @ Tue, 30 Jun 2020 00:02:15: 21000000 INFO @ Tue, 30 Jun 2020 00:02:17: 14000000 INFO @ Tue, 30 Jun 2020 00:02:20: 17000000 INFO @ Tue, 30 Jun 2020 00:02:22: 22000000 INFO @ Tue, 30 Jun 2020 00:02:23: 15000000 INFO @ Tue, 30 Jun 2020 00:02:28: 18000000 INFO @ Tue, 30 Jun 2020 00:02:28: 23000000 INFO @ Tue, 30 Jun 2020 00:02:29: 16000000 INFO @ Tue, 30 Jun 2020 00:02:35: 19000000 INFO @ Tue, 30 Jun 2020 00:02:35: 24000000 INFO @ Tue, 30 Jun 2020 00:02:36: 17000000 INFO @ Tue, 30 Jun 2020 00:02:42: 18000000 INFO @ Tue, 30 Jun 2020 00:02:42: 25000000 INFO @ Tue, 30 Jun 2020 00:02:42: 20000000 INFO @ Tue, 30 Jun 2020 00:02:48: 19000000 INFO @ Tue, 30 Jun 2020 00:02:49: 26000000 INFO @ Tue, 30 Jun 2020 00:02:50: 21000000 INFO @ Tue, 30 Jun 2020 00:02:54: 20000000 INFO @ Tue, 30 Jun 2020 00:02:56: 27000000 INFO @ Tue, 30 Jun 2020 00:02:57: 22000000 INFO @ Tue, 30 Jun 2020 00:03:00: 21000000 INFO @ Tue, 30 Jun 2020 00:03:03: 28000000 INFO @ Tue, 30 Jun 2020 00:03:04: 23000000 INFO @ Tue, 30 Jun 2020 00:03:06: 22000000 INFO @ Tue, 30 Jun 2020 00:03:10: 29000000 INFO @ Tue, 30 Jun 2020 00:03:11: 24000000 INFO @ Tue, 30 Jun 2020 00:03:12: 23000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 00:03:17: 30000000 INFO @ Tue, 30 Jun 2020 00:03:18: 25000000 INFO @ Tue, 30 Jun 2020 00:03:18: 24000000 INFO @ Tue, 30 Jun 2020 00:03:23: 31000000 INFO @ Tue, 30 Jun 2020 00:03:24: 25000000 INFO @ Tue, 30 Jun 2020 00:03:25: 26000000 INFO @ Tue, 30 Jun 2020 00:03:30: 26000000 INFO @ Tue, 30 Jun 2020 00:03:30: 32000000 INFO @ Tue, 30 Jun 2020 00:03:32: 27000000 INFO @ Tue, 30 Jun 2020 00:03:36: 27000000 INFO @ Tue, 30 Jun 2020 00:03:37: 33000000 INFO @ Tue, 30 Jun 2020 00:03:39: 28000000 INFO @ Tue, 30 Jun 2020 00:03:41: #1 tag size is determined as 51 bps INFO @ Tue, 30 Jun 2020 00:03:41: #1 tag size = 51 INFO @ Tue, 30 Jun 2020 00:03:41: #1 total tags in treatment: 33593885 INFO @ Tue, 30 Jun 2020 00:03:41: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:03:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:03:41: #1 tags after filtering in treatment: 33593885 INFO @ Tue, 30 Jun 2020 00:03:41: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:03:41: #1 finished! INFO @ Tue, 30 Jun 2020 00:03:41: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:03:41: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:03:42: 28000000 INFO @ Tue, 30 Jun 2020 00:03:43: #2 number of paired peaks: 23 WARNING @ Tue, 30 Jun 2020 00:03:43: Too few paired peaks (23) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:03:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981678/SRX3981678.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981678/SRX3981678.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981678/SRX3981678.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981678/SRX3981678.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:03:46: 29000000 INFO @ Tue, 30 Jun 2020 00:03:48: 29000000 INFO @ Tue, 30 Jun 2020 00:03:53: 30000000 INFO @ Tue, 30 Jun 2020 00:03:54: 30000000 INFO @ Tue, 30 Jun 2020 00:03:59: 31000000 INFO @ Tue, 30 Jun 2020 00:04:00: 31000000 INFO @ Tue, 30 Jun 2020 00:04:05: 32000000 INFO @ Tue, 30 Jun 2020 00:04:06: 32000000 INFO @ Tue, 30 Jun 2020 00:04:12: 33000000 INFO @ Tue, 30 Jun 2020 00:04:12: 33000000 INFO @ Tue, 30 Jun 2020 00:04:16: #1 tag size is determined as 51 bps INFO @ Tue, 30 Jun 2020 00:04:16: #1 tag size = 51 INFO @ Tue, 30 Jun 2020 00:04:16: #1 total tags in treatment: 33593885 INFO @ Tue, 30 Jun 2020 00:04:16: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:04:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:04:16: #1 tags after filtering in treatment: 33593885 INFO @ Tue, 30 Jun 2020 00:04:16: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:04:16: #1 finished! INFO @ Tue, 30 Jun 2020 00:04:16: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:04:16: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:04:16: #1 tag size is determined as 51 bps INFO @ Tue, 30 Jun 2020 00:04:16: #1 tag size = 51 INFO @ Tue, 30 Jun 2020 00:04:16: #1 total tags in treatment: 33593885 INFO @ Tue, 30 Jun 2020 00:04:16: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:04:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:04:17: #1 tags after filtering in treatment: 33593885 INFO @ Tue, 30 Jun 2020 00:04:17: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:04:17: #1 finished! INFO @ Tue, 30 Jun 2020 00:04:17: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:04:17: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:04:18: #2 number of paired peaks: 23 WARNING @ Tue, 30 Jun 2020 00:04:18: Too few paired peaks (23) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:04:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981678/SRX3981678.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981678/SRX3981678.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981678/SRX3981678.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981678/SRX3981678.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:04:19: #2 number of paired peaks: 23 WARNING @ Tue, 30 Jun 2020 00:04:19: Too few paired peaks (23) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:04:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981678/SRX3981678.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981678/SRX3981678.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981678/SRX3981678.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981678/SRX3981678.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。