Job ID = 6528057 SRX = SRX3981677 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:15:20 prefetch.2.10.7: 1) Downloading 'SRR7050415'... 2020-06-29T14:15:20 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:16:19 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:16:20 prefetch.2.10.7: 'SRR7050415' is valid 2020-06-29T14:16:20 prefetch.2.10.7: 1) 'SRR7050415' was downloaded successfully 2020-06-29T14:16:20 prefetch.2.10.7: 'SRR7050415' has 0 unresolved dependencies Read 11882720 spots for SRR7050415/SRR7050415.sra Written 11882720 spots for SRR7050415/SRR7050415.sra 2020-06-29T14:17:09 prefetch.2.10.7: 1) Downloading 'SRR7050416'... 2020-06-29T14:17:09 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:18:11 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:18:11 prefetch.2.10.7: 'SRR7050416' is valid 2020-06-29T14:18:11 prefetch.2.10.7: 1) 'SRR7050416' was downloaded successfully 2020-06-29T14:18:11 prefetch.2.10.7: 'SRR7050416' has 0 unresolved dependencies Read 11893274 spots for SRR7050416/SRR7050416.sra Written 11893274 spots for SRR7050416/SRR7050416.sra 2020-06-29T14:19:04 prefetch.2.10.7: 1) Downloading 'SRR7050417'... 2020-06-29T14:19:04 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:20:04 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:20:04 prefetch.2.10.7: 'SRR7050417' is valid 2020-06-29T14:20:04 prefetch.2.10.7: 1) 'SRR7050417' was downloaded successfully 2020-06-29T14:20:04 prefetch.2.10.7: 'SRR7050417' has 0 unresolved dependencies Read 11825531 spots for SRR7050417/SRR7050417.sra Written 11825531 spots for SRR7050417/SRR7050417.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:51 35601525 reads; of these: 35601525 (100.00%) were unpaired; of these: 956376 (2.69%) aligned 0 times 25235818 (70.88%) aligned exactly 1 time 9409331 (26.43%) aligned >1 times 97.31% overall alignment rate Time searching: 00:11:51 Overall time: 00:11:51 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 3767223 / 34645149 = 0.1087 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:48:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981677/SRX3981677.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981677/SRX3981677.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981677/SRX3981677.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981677/SRX3981677.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:48:15: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:48:15: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:48:21: 1000000 INFO @ Mon, 29 Jun 2020 23:48:27: 2000000 INFO @ Mon, 29 Jun 2020 23:48:33: 3000000 INFO @ Mon, 29 Jun 2020 23:48:39: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:48:44: 5000000 INFO @ Mon, 29 Jun 2020 23:48:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981677/SRX3981677.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981677/SRX3981677.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981677/SRX3981677.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981677/SRX3981677.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:48:45: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:48:45: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:48:51: 6000000 INFO @ Mon, 29 Jun 2020 23:48:51: 1000000 INFO @ Mon, 29 Jun 2020 23:48:57: 7000000 INFO @ Mon, 29 Jun 2020 23:48:58: 2000000 INFO @ Mon, 29 Jun 2020 23:49:03: 8000000 INFO @ Mon, 29 Jun 2020 23:49:04: 3000000 INFO @ Mon, 29 Jun 2020 23:49:09: 9000000 INFO @ Mon, 29 Jun 2020 23:49:10: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:49:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981677/SRX3981677.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981677/SRX3981677.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981677/SRX3981677.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981677/SRX3981677.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:49:15: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:49:15: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:49:15: 10000000 INFO @ Mon, 29 Jun 2020 23:49:16: 5000000 INFO @ Mon, 29 Jun 2020 23:49:21: 1000000 INFO @ Mon, 29 Jun 2020 23:49:21: 11000000 INFO @ Mon, 29 Jun 2020 23:49:23: 6000000 INFO @ Mon, 29 Jun 2020 23:49:27: 12000000 INFO @ Mon, 29 Jun 2020 23:49:27: 2000000 INFO @ Mon, 29 Jun 2020 23:49:29: 7000000 INFO @ Mon, 29 Jun 2020 23:49:33: 13000000 INFO @ Mon, 29 Jun 2020 23:49:34: 3000000 INFO @ Mon, 29 Jun 2020 23:49:35: 8000000 INFO @ Mon, 29 Jun 2020 23:49:39: 14000000 INFO @ Mon, 29 Jun 2020 23:49:40: 4000000 INFO @ Mon, 29 Jun 2020 23:49:41: 9000000 INFO @ Mon, 29 Jun 2020 23:49:45: 15000000 INFO @ Mon, 29 Jun 2020 23:49:46: 5000000 INFO @ Mon, 29 Jun 2020 23:49:47: 10000000 INFO @ Mon, 29 Jun 2020 23:49:52: 16000000 INFO @ Mon, 29 Jun 2020 23:49:52: 6000000 INFO @ Mon, 29 Jun 2020 23:49:53: 11000000 INFO @ Mon, 29 Jun 2020 23:49:58: 17000000 INFO @ Mon, 29 Jun 2020 23:49:58: 7000000 INFO @ Mon, 29 Jun 2020 23:49:59: 12000000 INFO @ Mon, 29 Jun 2020 23:50:04: 18000000 INFO @ Mon, 29 Jun 2020 23:50:04: 8000000 INFO @ Mon, 29 Jun 2020 23:50:05: 13000000 INFO @ Mon, 29 Jun 2020 23:50:10: 19000000 INFO @ Mon, 29 Jun 2020 23:50:10: 9000000 INFO @ Mon, 29 Jun 2020 23:50:11: 14000000 INFO @ Mon, 29 Jun 2020 23:50:16: 20000000 INFO @ Mon, 29 Jun 2020 23:50:16: 10000000 INFO @ Mon, 29 Jun 2020 23:50:16: 15000000 INFO @ Mon, 29 Jun 2020 23:50:22: 21000000 INFO @ Mon, 29 Jun 2020 23:50:22: 11000000 INFO @ Mon, 29 Jun 2020 23:50:23: 16000000 INFO @ Mon, 29 Jun 2020 23:50:28: 22000000 INFO @ Mon, 29 Jun 2020 23:50:28: 12000000 INFO @ Mon, 29 Jun 2020 23:50:29: 17000000 INFO @ Mon, 29 Jun 2020 23:50:34: 23000000 INFO @ Mon, 29 Jun 2020 23:50:34: 13000000 INFO @ Mon, 29 Jun 2020 23:50:35: 18000000 INFO @ Mon, 29 Jun 2020 23:50:40: 24000000 INFO @ Mon, 29 Jun 2020 23:50:40: 14000000 INFO @ Mon, 29 Jun 2020 23:50:41: 19000000 INFO @ Mon, 29 Jun 2020 23:50:46: 15000000 INFO @ Mon, 29 Jun 2020 23:50:46: 25000000 INFO @ Mon, 29 Jun 2020 23:50:47: 20000000 INFO @ Mon, 29 Jun 2020 23:50:52: 16000000 INFO @ Mon, 29 Jun 2020 23:50:53: 26000000 INFO @ Mon, 29 Jun 2020 23:50:53: 21000000 INFO @ Mon, 29 Jun 2020 23:50:59: 17000000 INFO @ Mon, 29 Jun 2020 23:50:59: 22000000 INFO @ Mon, 29 Jun 2020 23:50:59: 27000000 INFO @ Mon, 29 Jun 2020 23:51:05: 18000000 INFO @ Mon, 29 Jun 2020 23:51:05: 23000000 INFO @ Mon, 29 Jun 2020 23:51:06: 28000000 INFO @ Mon, 29 Jun 2020 23:51:11: 19000000 INFO @ Mon, 29 Jun 2020 23:51:12: 24000000 INFO @ Mon, 29 Jun 2020 23:51:12: 29000000 INFO @ Mon, 29 Jun 2020 23:51:17: 20000000 INFO @ Mon, 29 Jun 2020 23:51:18: 25000000 INFO @ Mon, 29 Jun 2020 23:51:18: 30000000 INFO @ Mon, 29 Jun 2020 23:51:23: 21000000 INFO @ Mon, 29 Jun 2020 23:51:24: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:51:24: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:51:24: #1 total tags in treatment: 30877926 INFO @ Mon, 29 Jun 2020 23:51:24: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:51:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:51:24: 26000000 INFO @ Mon, 29 Jun 2020 23:51:25: #1 tags after filtering in treatment: 30877926 INFO @ Mon, 29 Jun 2020 23:51:25: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:51:25: #1 finished! INFO @ Mon, 29 Jun 2020 23:51:25: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:51:25: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:51:26: #2 number of paired peaks: 39 WARNING @ Mon, 29 Jun 2020 23:51:26: Too few paired peaks (39) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:51:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981677/SRX3981677.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981677/SRX3981677.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981677/SRX3981677.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981677/SRX3981677.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:51:29: 22000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 23:51:30: 27000000 INFO @ Mon, 29 Jun 2020 23:51:35: 23000000 INFO @ Mon, 29 Jun 2020 23:51:36: 28000000 INFO @ Mon, 29 Jun 2020 23:51:42: 24000000 INFO @ Mon, 29 Jun 2020 23:51:42: 29000000 INFO @ Mon, 29 Jun 2020 23:51:47: 25000000 INFO @ Mon, 29 Jun 2020 23:51:48: 30000000 INFO @ Mon, 29 Jun 2020 23:51:53: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:51:53: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:51:53: #1 total tags in treatment: 30877926 INFO @ Mon, 29 Jun 2020 23:51:53: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:51:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:51:53: 26000000 INFO @ Mon, 29 Jun 2020 23:51:54: #1 tags after filtering in treatment: 30877926 INFO @ Mon, 29 Jun 2020 23:51:54: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:51:54: #1 finished! INFO @ Mon, 29 Jun 2020 23:51:54: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:51:54: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:51:56: #2 number of paired peaks: 39 WARNING @ Mon, 29 Jun 2020 23:51:56: Too few paired peaks (39) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:51:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981677/SRX3981677.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981677/SRX3981677.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981677/SRX3981677.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981677/SRX3981677.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:51:59: 27000000 INFO @ Mon, 29 Jun 2020 23:52:05: 28000000 INFO @ Mon, 29 Jun 2020 23:52:11: 29000000 INFO @ Mon, 29 Jun 2020 23:52:16: 30000000 INFO @ Mon, 29 Jun 2020 23:52:21: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:52:21: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:52:21: #1 total tags in treatment: 30877926 INFO @ Mon, 29 Jun 2020 23:52:21: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:52:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:52:22: #1 tags after filtering in treatment: 30877926 INFO @ Mon, 29 Jun 2020 23:52:22: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:52:22: #1 finished! INFO @ Mon, 29 Jun 2020 23:52:22: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:52:22: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:52:24: #2 number of paired peaks: 39 WARNING @ Mon, 29 Jun 2020 23:52:24: Too few paired peaks (39) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:52:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981677/SRX3981677.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981677/SRX3981677.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981677/SRX3981677.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981677/SRX3981677.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。