Job ID = 2590441 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 15,010,383 reads read : 30,020,766 reads written : 15,010,383 reads 0-length : 15,010,383 spots read : 15,019,898 reads read : 30,039,796 reads written : 15,019,898 reads 0-length : 15,019,898 spots read : 14,919,269 reads read : 29,838,538 reads written : 14,919,269 reads 0-length : 14,919,269 fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:15:23 44949550 reads; of these: 44949550 (100.00%) were unpaired; of these: 1424265 (3.17%) aligned 0 times 32605749 (72.54%) aligned exactly 1 time 10919536 (24.29%) aligned >1 times 96.83% overall alignment rate Time searching: 00:15:23 Overall time: 00:15:23 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 5013578 / 43525285 = 0.1152 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 12 Aug 2019 22:13:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981675/SRX3981675.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981675/SRX3981675.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981675/SRX3981675.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981675/SRX3981675.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 22:13:24: #1 read tag files... INFO @ Mon, 12 Aug 2019 22:13:24: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 22:13:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981675/SRX3981675.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981675/SRX3981675.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981675/SRX3981675.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981675/SRX3981675.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 22:13:24: #1 read tag files... INFO @ Mon, 12 Aug 2019 22:13:24: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 22:13:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981675/SRX3981675.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981675/SRX3981675.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981675/SRX3981675.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981675/SRX3981675.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 22:13:25: #1 read tag files... INFO @ Mon, 12 Aug 2019 22:13:25: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 22:13:33: 1000000 INFO @ Mon, 12 Aug 2019 22:13:34: 1000000 INFO @ Mon, 12 Aug 2019 22:13:34: 1000000 INFO @ Mon, 12 Aug 2019 22:13:43: 2000000 INFO @ Mon, 12 Aug 2019 22:13:43: 2000000 INFO @ Mon, 12 Aug 2019 22:13:43: 2000000 INFO @ Mon, 12 Aug 2019 22:13:51: 3000000 INFO @ Mon, 12 Aug 2019 22:13:52: 3000000 INFO @ Mon, 12 Aug 2019 22:13:52: 3000000 INFO @ Mon, 12 Aug 2019 22:14:00: 4000000 INFO @ Mon, 12 Aug 2019 22:14:01: 4000000 INFO @ Mon, 12 Aug 2019 22:14:01: 4000000 INFO @ Mon, 12 Aug 2019 22:14:08: 5000000 INFO @ Mon, 12 Aug 2019 22:14:10: 5000000 INFO @ Mon, 12 Aug 2019 22:14:10: 5000000 INFO @ Mon, 12 Aug 2019 22:14:17: 6000000 INFO @ Mon, 12 Aug 2019 22:14:18: 6000000 INFO @ Mon, 12 Aug 2019 22:14:18: 6000000 INFO @ Mon, 12 Aug 2019 22:14:25: 7000000 INFO @ Mon, 12 Aug 2019 22:14:27: 7000000 INFO @ Mon, 12 Aug 2019 22:14:27: 7000000 INFO @ Mon, 12 Aug 2019 22:14:33: 8000000 INFO @ Mon, 12 Aug 2019 22:14:35: 8000000 INFO @ Mon, 12 Aug 2019 22:14:36: 8000000 INFO @ Mon, 12 Aug 2019 22:14:42: 9000000 INFO @ Mon, 12 Aug 2019 22:14:44: 9000000 INFO @ Mon, 12 Aug 2019 22:14:44: 9000000 INFO @ Mon, 12 Aug 2019 22:14:50: 10000000 INFO @ Mon, 12 Aug 2019 22:14:52: 10000000 INFO @ Mon, 12 Aug 2019 22:14:53: 10000000 INFO @ Mon, 12 Aug 2019 22:14:58: 11000000 INFO @ Mon, 12 Aug 2019 22:15:01: 11000000 INFO @ Mon, 12 Aug 2019 22:15:01: 11000000 INFO @ Mon, 12 Aug 2019 22:15:07: 12000000 INFO @ Mon, 12 Aug 2019 22:15:09: 12000000 INFO @ Mon, 12 Aug 2019 22:15:10: 12000000 INFO @ Mon, 12 Aug 2019 22:15:15: 13000000 INFO @ Mon, 12 Aug 2019 22:15:18: 13000000 INFO @ Mon, 12 Aug 2019 22:15:18: 13000000 INFO @ Mon, 12 Aug 2019 22:15:23: 14000000 INFO @ Mon, 12 Aug 2019 22:15:27: 14000000 INFO @ Mon, 12 Aug 2019 22:15:27: 14000000 INFO @ Mon, 12 Aug 2019 22:15:32: 15000000 INFO @ Mon, 12 Aug 2019 22:15:35: 15000000 INFO @ Mon, 12 Aug 2019 22:15:35: 15000000 INFO @ Mon, 12 Aug 2019 22:15:40: 16000000 INFO @ Mon, 12 Aug 2019 22:15:44: 16000000 INFO @ Mon, 12 Aug 2019 22:15:44: 16000000 INFO @ Mon, 12 Aug 2019 22:15:49: 17000000 INFO @ Mon, 12 Aug 2019 22:15:52: 17000000 INFO @ Mon, 12 Aug 2019 22:15:53: 17000000 INFO @ Mon, 12 Aug 2019 22:15:57: 18000000 INFO @ Mon, 12 Aug 2019 22:16:01: 18000000 INFO @ Mon, 12 Aug 2019 22:16:01: 18000000 INFO @ Mon, 12 Aug 2019 22:16:05: 19000000 INFO @ Mon, 12 Aug 2019 22:16:09: 19000000 INFO @ Mon, 12 Aug 2019 22:16:10: 19000000 INFO @ Mon, 12 Aug 2019 22:16:14: 20000000 INFO @ Mon, 12 Aug 2019 22:16:18: 20000000 INFO @ Mon, 12 Aug 2019 22:16:18: 20000000 INFO @ Mon, 12 Aug 2019 22:16:22: 21000000 INFO @ Mon, 12 Aug 2019 22:16:26: 21000000 INFO @ Mon, 12 Aug 2019 22:16:27: 21000000 INFO @ Mon, 12 Aug 2019 22:16:31: 22000000 INFO @ Mon, 12 Aug 2019 22:16:35: 22000000 INFO @ Mon, 12 Aug 2019 22:16:35: 22000000 INFO @ Mon, 12 Aug 2019 22:16:39: 23000000 INFO @ Mon, 12 Aug 2019 22:16:43: 23000000 INFO @ Mon, 12 Aug 2019 22:16:44: 23000000 INFO @ Mon, 12 Aug 2019 22:16:48: 24000000 INFO @ Mon, 12 Aug 2019 22:16:51: 24000000 INFO @ Mon, 12 Aug 2019 22:16:52: 24000000 INFO @ Mon, 12 Aug 2019 22:16:56: 25000000 INFO @ Mon, 12 Aug 2019 22:17:00: 25000000 INFO @ Mon, 12 Aug 2019 22:17:01: 25000000 INFO @ Mon, 12 Aug 2019 22:17:05: 26000000 INFO @ Mon, 12 Aug 2019 22:17:09: 26000000 INFO @ Mon, 12 Aug 2019 22:17:09: 26000000 INFO @ Mon, 12 Aug 2019 22:17:14: 27000000 INFO @ Mon, 12 Aug 2019 22:17:17: 27000000 INFO @ Mon, 12 Aug 2019 22:17:18: 27000000 INFO @ Mon, 12 Aug 2019 22:17:22: 28000000 INFO @ Mon, 12 Aug 2019 22:17:26: 28000000 INFO @ Mon, 12 Aug 2019 22:17:26: 28000000 INFO @ Mon, 12 Aug 2019 22:17:30: 29000000 INFO @ Mon, 12 Aug 2019 22:17:34: 29000000 INFO @ Mon, 12 Aug 2019 22:17:35: 29000000 INFO @ Mon, 12 Aug 2019 22:17:39: 30000000 INFO @ Mon, 12 Aug 2019 22:17:43: 30000000 INFO @ Mon, 12 Aug 2019 22:17:43: 30000000 INFO @ Mon, 12 Aug 2019 22:17:47: 31000000 INFO @ Mon, 12 Aug 2019 22:17:51: 31000000 INFO @ Mon, 12 Aug 2019 22:17:52: 31000000 INFO @ Mon, 12 Aug 2019 22:17:55: 32000000 INFO @ Mon, 12 Aug 2019 22:18:00: 32000000 INFO @ Mon, 12 Aug 2019 22:18:00: 32000000 INFO @ Mon, 12 Aug 2019 22:18:04: 33000000 INFO @ Mon, 12 Aug 2019 22:18:08: 33000000 INFO @ Mon, 12 Aug 2019 22:18:09: 33000000 INFO @ Mon, 12 Aug 2019 22:18:12: 34000000 INFO @ Mon, 12 Aug 2019 22:18:17: 34000000 INFO @ Mon, 12 Aug 2019 22:18:17: 34000000 INFO @ Mon, 12 Aug 2019 22:18:21: 35000000 INFO @ Mon, 12 Aug 2019 22:18:25: 35000000 INFO @ Mon, 12 Aug 2019 22:18:26: 35000000 INFO @ Mon, 12 Aug 2019 22:18:29: 36000000 INFO @ Mon, 12 Aug 2019 22:18:34: 36000000 INFO @ Mon, 12 Aug 2019 22:18:34: 36000000 INFO @ Mon, 12 Aug 2019 22:18:38: 37000000 INFO @ Mon, 12 Aug 2019 22:18:42: 37000000 INFO @ Mon, 12 Aug 2019 22:18:43: 37000000 INFO @ Mon, 12 Aug 2019 22:18:46: 38000000 INFO @ Mon, 12 Aug 2019 22:18:51: #1 tag size is determined as 51 bps INFO @ Mon, 12 Aug 2019 22:18:51: #1 tag size = 51 INFO @ Mon, 12 Aug 2019 22:18:51: #1 total tags in treatment: 38511707 INFO @ Mon, 12 Aug 2019 22:18:51: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 22:18:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 22:18:51: 38000000 INFO @ Mon, 12 Aug 2019 22:18:51: #1 tags after filtering in treatment: 38511707 INFO @ Mon, 12 Aug 2019 22:18:51: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 22:18:51: #1 finished! INFO @ Mon, 12 Aug 2019 22:18:51: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 22:18:51: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 22:18:52: 38000000 INFO @ Mon, 12 Aug 2019 22:18:55: #2 number of paired peaks: 3 WARNING @ Mon, 12 Aug 2019 22:18:55: Too few paired peaks (3) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 22:18:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981675/SRX3981675.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981675/SRX3981675.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981675/SRX3981675.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981675/SRX3981675.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 22:18:55: #1 tag size is determined as 51 bps INFO @ Mon, 12 Aug 2019 22:18:55: #1 tag size = 51 INFO @ Mon, 12 Aug 2019 22:18:55: #1 total tags in treatment: 38511707 INFO @ Mon, 12 Aug 2019 22:18:55: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 22:18:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 22:18:56: #1 tag size is determined as 51 bps INFO @ Mon, 12 Aug 2019 22:18:56: #1 tag size = 51 INFO @ Mon, 12 Aug 2019 22:18:56: #1 total tags in treatment: 38511707 INFO @ Mon, 12 Aug 2019 22:18:56: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 22:18:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 22:18:56: #1 tags after filtering in treatment: 38511707 INFO @ Mon, 12 Aug 2019 22:18:56: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 22:18:56: #1 finished! INFO @ Mon, 12 Aug 2019 22:18:56: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 22:18:56: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 22:18:57: #1 tags after filtering in treatment: 38511707 INFO @ Mon, 12 Aug 2019 22:18:57: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 22:18:57: #1 finished! INFO @ Mon, 12 Aug 2019 22:18:57: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 22:18:57: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 22:18:59: #2 number of paired peaks: 3 WARNING @ Mon, 12 Aug 2019 22:18:59: Too few paired peaks (3) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 22:18:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981675/SRX3981675.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981675/SRX3981675.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981675/SRX3981675.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981675/SRX3981675.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 22:19:00: #2 number of paired peaks: 3 WARNING @ Mon, 12 Aug 2019 22:19:00: Too few paired peaks (3) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 22:19:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981675/SRX3981675.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981675/SRX3981675.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981675/SRX3981675.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981675/SRX3981675.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。