Job ID = 2590440 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 14,848,947 reads read : 29,697,894 reads written : 14,848,947 reads 0-length : 14,848,947 spots read : 14,848,303 reads read : 29,696,606 reads written : 14,848,303 reads 0-length : 14,848,303 spots read : 14,744,519 reads read : 29,489,038 reads written : 14,744,519 reads 0-length : 14,744,519 fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:17:04 44441769 reads; of these: 44441769 (100.00%) were unpaired; of these: 1181670 (2.66%) aligned 0 times 32224500 (72.51%) aligned exactly 1 time 11035599 (24.83%) aligned >1 times 97.34% overall alignment rate Time searching: 00:17:04 Overall time: 00:17:04 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 5085344 / 43260099 = 0.1176 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 12 Aug 2019 22:12:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981674/SRX3981674.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981674/SRX3981674.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981674/SRX3981674.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981674/SRX3981674.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 22:12:44: #1 read tag files... INFO @ Mon, 12 Aug 2019 22:12:44: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 22:12:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981674/SRX3981674.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981674/SRX3981674.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981674/SRX3981674.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981674/SRX3981674.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 22:12:45: #1 read tag files... INFO @ Mon, 12 Aug 2019 22:12:45: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 22:12:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981674/SRX3981674.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981674/SRX3981674.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981674/SRX3981674.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981674/SRX3981674.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 22:12:45: #1 read tag files... INFO @ Mon, 12 Aug 2019 22:12:45: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 22:12:52: 1000000 INFO @ Mon, 12 Aug 2019 22:12:52: 1000000 INFO @ Mon, 12 Aug 2019 22:12:53: 1000000 INFO @ Mon, 12 Aug 2019 22:12:59: 2000000 INFO @ Mon, 12 Aug 2019 22:13:00: 2000000 INFO @ Mon, 12 Aug 2019 22:13:01: 2000000 INFO @ Mon, 12 Aug 2019 22:13:07: 3000000 INFO @ Mon, 12 Aug 2019 22:13:07: 3000000 INFO @ Mon, 12 Aug 2019 22:13:08: 3000000 INFO @ Mon, 12 Aug 2019 22:13:15: 4000000 INFO @ Mon, 12 Aug 2019 22:13:15: 4000000 INFO @ Mon, 12 Aug 2019 22:13:15: 4000000 INFO @ Mon, 12 Aug 2019 22:13:22: 5000000 INFO @ Mon, 12 Aug 2019 22:13:23: 5000000 INFO @ Mon, 12 Aug 2019 22:13:23: 5000000 INFO @ Mon, 12 Aug 2019 22:13:29: 6000000 INFO @ Mon, 12 Aug 2019 22:13:30: 6000000 INFO @ Mon, 12 Aug 2019 22:13:31: 6000000 INFO @ Mon, 12 Aug 2019 22:13:36: 7000000 INFO @ Mon, 12 Aug 2019 22:13:37: 7000000 INFO @ Mon, 12 Aug 2019 22:13:39: 7000000 INFO @ Mon, 12 Aug 2019 22:13:43: 8000000 INFO @ Mon, 12 Aug 2019 22:13:44: 8000000 INFO @ Mon, 12 Aug 2019 22:13:47: 8000000 INFO @ Mon, 12 Aug 2019 22:13:51: 9000000 INFO @ Mon, 12 Aug 2019 22:13:51: 9000000 INFO @ Mon, 12 Aug 2019 22:13:54: 9000000 INFO @ Mon, 12 Aug 2019 22:13:58: 10000000 INFO @ Mon, 12 Aug 2019 22:13:58: 10000000 INFO @ Mon, 12 Aug 2019 22:14:02: 10000000 INFO @ Mon, 12 Aug 2019 22:14:05: 11000000 INFO @ Mon, 12 Aug 2019 22:14:05: 11000000 INFO @ Mon, 12 Aug 2019 22:14:10: 11000000 INFO @ Mon, 12 Aug 2019 22:14:12: 12000000 INFO @ Mon, 12 Aug 2019 22:14:12: 12000000 INFO @ Mon, 12 Aug 2019 22:14:18: 12000000 INFO @ Mon, 12 Aug 2019 22:14:19: 13000000 INFO @ Mon, 12 Aug 2019 22:14:19: 13000000 INFO @ Mon, 12 Aug 2019 22:14:25: 13000000 INFO @ Mon, 12 Aug 2019 22:14:26: 14000000 INFO @ Mon, 12 Aug 2019 22:14:26: 14000000 INFO @ Mon, 12 Aug 2019 22:14:33: 14000000 INFO @ Mon, 12 Aug 2019 22:14:33: 15000000 INFO @ Mon, 12 Aug 2019 22:14:33: 15000000 INFO @ Mon, 12 Aug 2019 22:14:40: 16000000 INFO @ Mon, 12 Aug 2019 22:14:41: 16000000 INFO @ Mon, 12 Aug 2019 22:14:41: 15000000 INFO @ Mon, 12 Aug 2019 22:14:47: 17000000 INFO @ Mon, 12 Aug 2019 22:14:48: 17000000 INFO @ Mon, 12 Aug 2019 22:14:49: 16000000 INFO @ Mon, 12 Aug 2019 22:14:54: 18000000 INFO @ Mon, 12 Aug 2019 22:14:57: 18000000 INFO @ Mon, 12 Aug 2019 22:14:58: 17000000 INFO @ Mon, 12 Aug 2019 22:15:01: 19000000 INFO @ Mon, 12 Aug 2019 22:15:04: 19000000 INFO @ Mon, 12 Aug 2019 22:15:06: 18000000 INFO @ Mon, 12 Aug 2019 22:15:11: 20000000 INFO @ Mon, 12 Aug 2019 22:15:12: 20000000 INFO @ Mon, 12 Aug 2019 22:15:15: 19000000 INFO @ Mon, 12 Aug 2019 22:15:19: 21000000 INFO @ Mon, 12 Aug 2019 22:15:21: 21000000 INFO @ Mon, 12 Aug 2019 22:15:24: 20000000 INFO @ Mon, 12 Aug 2019 22:15:26: 22000000 INFO @ Mon, 12 Aug 2019 22:15:31: 22000000 INFO @ Mon, 12 Aug 2019 22:15:33: 23000000 INFO @ Mon, 12 Aug 2019 22:15:34: 21000000 INFO @ Mon, 12 Aug 2019 22:15:40: 24000000 INFO @ Mon, 12 Aug 2019 22:15:41: 23000000 INFO @ Mon, 12 Aug 2019 22:15:43: 22000000 INFO @ Mon, 12 Aug 2019 22:15:47: 25000000 INFO @ Mon, 12 Aug 2019 22:15:51: 24000000 INFO @ Mon, 12 Aug 2019 22:15:52: 23000000 INFO @ Mon, 12 Aug 2019 22:15:54: 26000000 INFO @ Mon, 12 Aug 2019 22:16:01: 24000000 INFO @ Mon, 12 Aug 2019 22:16:01: 25000000 INFO @ Mon, 12 Aug 2019 22:16:01: 27000000 INFO @ Mon, 12 Aug 2019 22:16:08: 28000000 INFO @ Mon, 12 Aug 2019 22:16:09: 25000000 INFO @ Mon, 12 Aug 2019 22:16:10: 26000000 INFO @ Mon, 12 Aug 2019 22:16:15: 29000000 INFO @ Mon, 12 Aug 2019 22:16:19: 26000000 INFO @ Mon, 12 Aug 2019 22:16:20: 27000000 INFO @ Mon, 12 Aug 2019 22:16:22: 30000000 INFO @ Mon, 12 Aug 2019 22:16:27: 27000000 INFO @ Mon, 12 Aug 2019 22:16:29: 28000000 INFO @ Mon, 12 Aug 2019 22:16:29: 31000000 INFO @ Mon, 12 Aug 2019 22:16:36: 32000000 INFO @ Mon, 12 Aug 2019 22:16:36: 28000000 INFO @ Mon, 12 Aug 2019 22:16:38: 29000000 INFO @ Mon, 12 Aug 2019 22:16:43: 33000000 INFO @ Mon, 12 Aug 2019 22:16:45: 29000000 INFO @ Mon, 12 Aug 2019 22:16:47: 30000000 INFO @ Mon, 12 Aug 2019 22:16:50: 34000000 INFO @ Mon, 12 Aug 2019 22:16:54: 30000000 INFO @ Mon, 12 Aug 2019 22:16:56: 31000000 INFO @ Mon, 12 Aug 2019 22:16:57: 35000000 INFO @ Mon, 12 Aug 2019 22:17:03: 31000000 INFO @ Mon, 12 Aug 2019 22:17:04: 36000000 INFO @ Mon, 12 Aug 2019 22:17:04: 32000000 INFO @ Mon, 12 Aug 2019 22:17:11: 37000000 INFO @ Mon, 12 Aug 2019 22:17:11: 32000000 INFO @ Mon, 12 Aug 2019 22:17:13: 33000000 INFO @ Mon, 12 Aug 2019 22:17:18: 38000000 INFO @ Mon, 12 Aug 2019 22:17:20: #1 tag size is determined as 51 bps INFO @ Mon, 12 Aug 2019 22:17:20: #1 tag size = 51 INFO @ Mon, 12 Aug 2019 22:17:20: #1 total tags in treatment: 38174755 INFO @ Mon, 12 Aug 2019 22:17:20: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 22:17:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 22:17:20: 33000000 INFO @ Mon, 12 Aug 2019 22:17:20: #1 tags after filtering in treatment: 38174755 INFO @ Mon, 12 Aug 2019 22:17:20: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 22:17:20: #1 finished! INFO @ Mon, 12 Aug 2019 22:17:20: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 22:17:20: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 22:17:22: 34000000 INFO @ Mon, 12 Aug 2019 22:17:24: #2 number of paired peaks: 2 WARNING @ Mon, 12 Aug 2019 22:17:24: Too few paired peaks (2) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 22:17:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981674/SRX3981674.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981674/SRX3981674.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981674/SRX3981674.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981674/SRX3981674.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 22:17:29: 34000000 INFO @ Mon, 12 Aug 2019 22:17:30: 35000000 INFO @ Mon, 12 Aug 2019 22:17:37: 35000000 INFO @ Mon, 12 Aug 2019 22:17:39: 36000000 INFO @ Mon, 12 Aug 2019 22:17:46: 36000000 INFO @ Mon, 12 Aug 2019 22:17:48: 37000000 INFO @ Mon, 12 Aug 2019 22:17:55: 37000000 INFO @ Mon, 12 Aug 2019 22:17:56: 38000000 INFO @ Mon, 12 Aug 2019 22:17:58: #1 tag size is determined as 51 bps INFO @ Mon, 12 Aug 2019 22:17:58: #1 tag size = 51 INFO @ Mon, 12 Aug 2019 22:17:58: #1 total tags in treatment: 38174755 INFO @ Mon, 12 Aug 2019 22:17:58: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 22:17:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 22:17:59: #1 tags after filtering in treatment: 38174755 INFO @ Mon, 12 Aug 2019 22:17:59: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 22:17:59: #1 finished! INFO @ Mon, 12 Aug 2019 22:17:59: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 22:17:59: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 22:18:02: #2 number of paired peaks: 2 WARNING @ Mon, 12 Aug 2019 22:18:02: Too few paired peaks (2) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 22:18:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981674/SRX3981674.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981674/SRX3981674.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981674/SRX3981674.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981674/SRX3981674.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 22:18:03: 38000000 INFO @ Mon, 12 Aug 2019 22:18:04: #1 tag size is determined as 51 bps INFO @ Mon, 12 Aug 2019 22:18:04: #1 tag size = 51 INFO @ Mon, 12 Aug 2019 22:18:04: #1 total tags in treatment: 38174755 INFO @ Mon, 12 Aug 2019 22:18:04: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 22:18:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 22:18:05: #1 tags after filtering in treatment: 38174755 INFO @ Mon, 12 Aug 2019 22:18:05: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 22:18:05: #1 finished! INFO @ Mon, 12 Aug 2019 22:18:05: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 22:18:05: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 22:18:08: #2 number of paired peaks: 2 WARNING @ Mon, 12 Aug 2019 22:18:08: Too few paired peaks (2) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 22:18:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981674/SRX3981674.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981674/SRX3981674.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981674/SRX3981674.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981674/SRX3981674.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。