Job ID = 6528054 SRX = SRX3981673 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:22:22 prefetch.2.10.7: 1) Downloading 'SRR7050403'... 2020-06-29T14:22:22 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:23:24 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:23:25 prefetch.2.10.7: 'SRR7050403' is valid 2020-06-29T14:23:25 prefetch.2.10.7: 1) 'SRR7050403' was downloaded successfully 2020-06-29T14:23:25 prefetch.2.10.7: 'SRR7050403' has 0 unresolved dependencies Read 13282132 spots for SRR7050403/SRR7050403.sra Written 13282132 spots for SRR7050403/SRR7050403.sra 2020-06-29T14:24:22 prefetch.2.10.7: 1) Downloading 'SRR7050404'... 2020-06-29T14:24:22 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:25:15 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:25:15 prefetch.2.10.7: 'SRR7050404' is valid 2020-06-29T14:25:15 prefetch.2.10.7: 1) 'SRR7050404' was downloaded successfully 2020-06-29T14:25:15 prefetch.2.10.7: 'SRR7050404' has 0 unresolved dependencies Read 13296709 spots for SRR7050404/SRR7050404.sra Written 13296709 spots for SRR7050404/SRR7050404.sra 2020-06-29T14:26:09 prefetch.2.10.7: 1) Downloading 'SRR7050405'... 2020-06-29T14:26:09 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:27:33 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:27:33 prefetch.2.10.7: 'SRR7050405' is valid 2020-06-29T14:27:33 prefetch.2.10.7: 1) 'SRR7050405' was downloaded successfully 2020-06-29T14:27:33 prefetch.2.10.7: 'SRR7050405' has 0 unresolved dependencies Read 13215111 spots for SRR7050405/SRR7050405.sra Written 13215111 spots for SRR7050405/SRR7050405.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:12:30 39793952 reads; of these: 39793952 (100.00%) were unpaired; of these: 1041472 (2.62%) aligned 0 times 28617837 (71.92%) aligned exactly 1 time 10134643 (25.47%) aligned >1 times 97.38% overall alignment rate Time searching: 00:12:30 Overall time: 00:12:30 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 4418587 / 38752480 = 0.1140 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:58:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981673/SRX3981673.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981673/SRX3981673.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981673/SRX3981673.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981673/SRX3981673.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:58:32: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:58:32: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:58:38: 1000000 INFO @ Mon, 29 Jun 2020 23:58:45: 2000000 INFO @ Mon, 29 Jun 2020 23:58:52: 3000000 INFO @ Mon, 29 Jun 2020 23:58:59: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:59:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981673/SRX3981673.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981673/SRX3981673.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981673/SRX3981673.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981673/SRX3981673.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:59:02: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:59:02: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:59:06: 5000000 INFO @ Mon, 29 Jun 2020 23:59:10: 1000000 INFO @ Mon, 29 Jun 2020 23:59:14: 6000000 INFO @ Mon, 29 Jun 2020 23:59:18: 2000000 INFO @ Mon, 29 Jun 2020 23:59:22: 7000000 INFO @ Mon, 29 Jun 2020 23:59:25: 3000000 INFO @ Mon, 29 Jun 2020 23:59:29: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:59:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981673/SRX3981673.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981673/SRX3981673.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981673/SRX3981673.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981673/SRX3981673.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:59:32: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:59:32: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:59:33: 4000000 INFO @ Mon, 29 Jun 2020 23:59:37: 9000000 INFO @ Mon, 29 Jun 2020 23:59:40: 1000000 INFO @ Mon, 29 Jun 2020 23:59:42: 5000000 INFO @ Mon, 29 Jun 2020 23:59:45: 10000000 INFO @ Mon, 29 Jun 2020 23:59:47: 2000000 INFO @ Mon, 29 Jun 2020 23:59:50: 6000000 INFO @ Mon, 29 Jun 2020 23:59:53: 11000000 INFO @ Mon, 29 Jun 2020 23:59:54: 3000000 INFO @ Mon, 29 Jun 2020 23:59:58: 7000000 INFO @ Tue, 30 Jun 2020 00:00:01: 12000000 INFO @ Tue, 30 Jun 2020 00:00:01: 4000000 INFO @ Tue, 30 Jun 2020 00:00:06: 8000000 INFO @ Tue, 30 Jun 2020 00:00:09: 5000000 INFO @ Tue, 30 Jun 2020 00:00:10: 13000000 INFO @ Tue, 30 Jun 2020 00:00:14: 9000000 INFO @ Tue, 30 Jun 2020 00:00:17: 6000000 INFO @ Tue, 30 Jun 2020 00:00:18: 14000000 INFO @ Tue, 30 Jun 2020 00:00:22: 10000000 INFO @ Tue, 30 Jun 2020 00:00:25: 7000000 INFO @ Tue, 30 Jun 2020 00:00:26: 15000000 INFO @ Tue, 30 Jun 2020 00:00:30: 11000000 INFO @ Tue, 30 Jun 2020 00:00:32: 8000000 INFO @ Tue, 30 Jun 2020 00:00:34: 16000000 INFO @ Tue, 30 Jun 2020 00:00:37: 12000000 INFO @ Tue, 30 Jun 2020 00:00:40: 9000000 INFO @ Tue, 30 Jun 2020 00:00:42: 17000000 INFO @ Tue, 30 Jun 2020 00:00:45: 13000000 INFO @ Tue, 30 Jun 2020 00:00:48: 10000000 INFO @ Tue, 30 Jun 2020 00:00:50: 18000000 INFO @ Tue, 30 Jun 2020 00:00:53: 14000000 INFO @ Tue, 30 Jun 2020 00:00:55: 11000000 INFO @ Tue, 30 Jun 2020 00:00:58: 19000000 INFO @ Tue, 30 Jun 2020 00:01:01: 15000000 INFO @ Tue, 30 Jun 2020 00:01:02: 12000000 INFO @ Tue, 30 Jun 2020 00:01:07: 20000000 INFO @ Tue, 30 Jun 2020 00:01:09: 16000000 INFO @ Tue, 30 Jun 2020 00:01:10: 13000000 INFO @ Tue, 30 Jun 2020 00:01:14: 21000000 INFO @ Tue, 30 Jun 2020 00:01:17: 17000000 INFO @ Tue, 30 Jun 2020 00:01:17: 14000000 INFO @ Tue, 30 Jun 2020 00:01:22: 22000000 INFO @ Tue, 30 Jun 2020 00:01:25: 15000000 INFO @ Tue, 30 Jun 2020 00:01:26: 18000000 INFO @ Tue, 30 Jun 2020 00:01:30: 23000000 INFO @ Tue, 30 Jun 2020 00:01:32: 16000000 INFO @ Tue, 30 Jun 2020 00:01:34: 19000000 INFO @ Tue, 30 Jun 2020 00:01:38: 24000000 INFO @ Tue, 30 Jun 2020 00:01:39: 17000000 INFO @ Tue, 30 Jun 2020 00:01:42: 20000000 INFO @ Tue, 30 Jun 2020 00:01:45: 25000000 INFO @ Tue, 30 Jun 2020 00:01:47: 18000000 INFO @ Tue, 30 Jun 2020 00:01:50: 21000000 INFO @ Tue, 30 Jun 2020 00:01:53: 26000000 INFO @ Tue, 30 Jun 2020 00:01:54: 19000000 INFO @ Tue, 30 Jun 2020 00:01:58: 22000000 INFO @ Tue, 30 Jun 2020 00:02:01: 27000000 INFO @ Tue, 30 Jun 2020 00:02:01: 20000000 INFO @ Tue, 30 Jun 2020 00:02:07: 23000000 INFO @ Tue, 30 Jun 2020 00:02:08: 21000000 INFO @ Tue, 30 Jun 2020 00:02:09: 28000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 00:02:15: 24000000 INFO @ Tue, 30 Jun 2020 00:02:15: 22000000 INFO @ Tue, 30 Jun 2020 00:02:16: 29000000 INFO @ Tue, 30 Jun 2020 00:02:22: 23000000 INFO @ Tue, 30 Jun 2020 00:02:22: 25000000 INFO @ Tue, 30 Jun 2020 00:02:24: 30000000 INFO @ Tue, 30 Jun 2020 00:02:29: 24000000 INFO @ Tue, 30 Jun 2020 00:02:30: 26000000 INFO @ Tue, 30 Jun 2020 00:02:32: 31000000 INFO @ Tue, 30 Jun 2020 00:02:36: 25000000 INFO @ Tue, 30 Jun 2020 00:02:38: 27000000 INFO @ Tue, 30 Jun 2020 00:02:39: 32000000 INFO @ Tue, 30 Jun 2020 00:02:43: 26000000 INFO @ Tue, 30 Jun 2020 00:02:46: 28000000 INFO @ Tue, 30 Jun 2020 00:02:47: 33000000 INFO @ Tue, 30 Jun 2020 00:02:50: 27000000 INFO @ Tue, 30 Jun 2020 00:02:54: 34000000 INFO @ Tue, 30 Jun 2020 00:02:55: 29000000 INFO @ Tue, 30 Jun 2020 00:02:57: #1 tag size is determined as 51 bps INFO @ Tue, 30 Jun 2020 00:02:57: #1 tag size = 51 INFO @ Tue, 30 Jun 2020 00:02:57: #1 total tags in treatment: 34333893 INFO @ Tue, 30 Jun 2020 00:02:57: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:02:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:02:57: 28000000 INFO @ Tue, 30 Jun 2020 00:02:58: #1 tags after filtering in treatment: 34333893 INFO @ Tue, 30 Jun 2020 00:02:58: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:02:58: #1 finished! INFO @ Tue, 30 Jun 2020 00:02:58: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:02:58: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:03:00: #2 number of paired peaks: 21 WARNING @ Tue, 30 Jun 2020 00:03:00: Too few paired peaks (21) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:03:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981673/SRX3981673.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981673/SRX3981673.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981673/SRX3981673.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981673/SRX3981673.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:03:03: 30000000 INFO @ Tue, 30 Jun 2020 00:03:04: 29000000 INFO @ Tue, 30 Jun 2020 00:03:11: 31000000 INFO @ Tue, 30 Jun 2020 00:03:11: 30000000 INFO @ Tue, 30 Jun 2020 00:03:18: 31000000 INFO @ Tue, 30 Jun 2020 00:03:19: 32000000 BigWig に変換しました。 INFO @ Tue, 30 Jun 2020 00:03:25: 32000000 INFO @ Tue, 30 Jun 2020 00:03:26: 33000000 INFO @ Tue, 30 Jun 2020 00:03:32: 33000000 INFO @ Tue, 30 Jun 2020 00:03:34: 34000000 INFO @ Tue, 30 Jun 2020 00:03:37: #1 tag size is determined as 51 bps INFO @ Tue, 30 Jun 2020 00:03:37: #1 tag size = 51 INFO @ Tue, 30 Jun 2020 00:03:37: #1 total tags in treatment: 34333893 INFO @ Tue, 30 Jun 2020 00:03:37: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:03:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:03:38: #1 tags after filtering in treatment: 34333893 INFO @ Tue, 30 Jun 2020 00:03:38: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:03:38: #1 finished! INFO @ Tue, 30 Jun 2020 00:03:38: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:03:38: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:03:38: 34000000 INFO @ Tue, 30 Jun 2020 00:03:40: #2 number of paired peaks: 21 WARNING @ Tue, 30 Jun 2020 00:03:40: Too few paired peaks (21) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:03:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981673/SRX3981673.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981673/SRX3981673.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981673/SRX3981673.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981673/SRX3981673.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:03:41: #1 tag size is determined as 51 bps INFO @ Tue, 30 Jun 2020 00:03:41: #1 tag size = 51 INFO @ Tue, 30 Jun 2020 00:03:41: #1 total tags in treatment: 34333893 INFO @ Tue, 30 Jun 2020 00:03:41: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:03:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:03:41: #1 tags after filtering in treatment: 34333893 INFO @ Tue, 30 Jun 2020 00:03:41: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:03:41: #1 finished! INFO @ Tue, 30 Jun 2020 00:03:41: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:03:41: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:03:43: #2 number of paired peaks: 21 WARNING @ Tue, 30 Jun 2020 00:03:43: Too few paired peaks (21) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:03:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981673/SRX3981673.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981673/SRX3981673.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981673/SRX3981673.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981673/SRX3981673.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling