Job ID = 2590436 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 14,148,098 reads read : 28,296,196 reads written : 14,148,098 reads 0-length : 14,148,098 spots read : 14,140,581 reads read : 28,281,162 reads written : 14,140,581 reads 0-length : 14,140,581 spots read : 14,055,217 reads read : 28,110,434 reads written : 14,055,217 reads 0-length : 14,055,217 fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:16:09 42343896 reads; of these: 42343896 (100.00%) were unpaired; of these: 1194103 (2.82%) aligned 0 times 30161375 (71.23%) aligned exactly 1 time 10988418 (25.95%) aligned >1 times 97.18% overall alignment rate Time searching: 00:16:09 Overall time: 00:16:09 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 4858810 / 41149793 = 0.1181 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 12 Aug 2019 21:53:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981670/SRX3981670.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981670/SRX3981670.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981670/SRX3981670.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981670/SRX3981670.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 21:53:43: #1 read tag files... INFO @ Mon, 12 Aug 2019 21:53:43: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 21:53:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981670/SRX3981670.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981670/SRX3981670.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981670/SRX3981670.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981670/SRX3981670.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 21:53:44: #1 read tag files... INFO @ Mon, 12 Aug 2019 21:53:44: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 21:53:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981670/SRX3981670.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981670/SRX3981670.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981670/SRX3981670.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981670/SRX3981670.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 21:53:45: #1 read tag files... INFO @ Mon, 12 Aug 2019 21:53:45: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 21:53:51: 1000000 INFO @ Mon, 12 Aug 2019 21:53:51: 1000000 INFO @ Mon, 12 Aug 2019 21:53:53: 1000000 INFO @ Mon, 12 Aug 2019 21:53:58: 2000000 INFO @ Mon, 12 Aug 2019 21:53:58: 2000000 INFO @ Mon, 12 Aug 2019 21:54:00: 2000000 INFO @ Mon, 12 Aug 2019 21:54:04: 3000000 INFO @ Mon, 12 Aug 2019 21:54:05: 3000000 INFO @ Mon, 12 Aug 2019 21:54:07: 3000000 INFO @ Mon, 12 Aug 2019 21:54:11: 4000000 INFO @ Mon, 12 Aug 2019 21:54:13: 4000000 INFO @ Mon, 12 Aug 2019 21:54:15: 4000000 INFO @ Mon, 12 Aug 2019 21:54:18: 5000000 INFO @ Mon, 12 Aug 2019 21:54:20: 5000000 INFO @ Mon, 12 Aug 2019 21:54:22: 5000000 INFO @ Mon, 12 Aug 2019 21:54:24: 6000000 INFO @ Mon, 12 Aug 2019 21:54:27: 6000000 INFO @ Mon, 12 Aug 2019 21:54:29: 6000000 INFO @ Mon, 12 Aug 2019 21:54:31: 7000000 INFO @ Mon, 12 Aug 2019 21:54:34: 7000000 INFO @ Mon, 12 Aug 2019 21:54:36: 7000000 INFO @ Mon, 12 Aug 2019 21:54:38: 8000000 INFO @ Mon, 12 Aug 2019 21:54:42: 8000000 INFO @ Mon, 12 Aug 2019 21:54:44: 8000000 INFO @ Mon, 12 Aug 2019 21:54:44: 9000000 INFO @ Mon, 12 Aug 2019 21:54:49: 9000000 INFO @ Mon, 12 Aug 2019 21:54:51: 10000000 INFO @ Mon, 12 Aug 2019 21:54:51: 9000000 INFO @ Mon, 12 Aug 2019 21:54:57: 10000000 INFO @ Mon, 12 Aug 2019 21:54:57: 11000000 INFO @ Mon, 12 Aug 2019 21:54:58: 10000000 INFO @ Mon, 12 Aug 2019 21:55:04: 12000000 INFO @ Mon, 12 Aug 2019 21:55:04: 11000000 INFO @ Mon, 12 Aug 2019 21:55:06: 11000000 INFO @ Mon, 12 Aug 2019 21:55:11: 13000000 INFO @ Mon, 12 Aug 2019 21:55:12: 12000000 INFO @ Mon, 12 Aug 2019 21:55:13: 12000000 INFO @ Mon, 12 Aug 2019 21:55:17: 14000000 INFO @ Mon, 12 Aug 2019 21:55:19: 13000000 INFO @ Mon, 12 Aug 2019 21:55:20: 13000000 INFO @ Mon, 12 Aug 2019 21:55:24: 15000000 INFO @ Mon, 12 Aug 2019 21:55:26: 14000000 INFO @ Mon, 12 Aug 2019 21:55:27: 14000000 INFO @ Mon, 12 Aug 2019 21:55:30: 16000000 INFO @ Mon, 12 Aug 2019 21:55:34: 15000000 INFO @ Mon, 12 Aug 2019 21:55:34: 15000000 INFO @ Mon, 12 Aug 2019 21:55:37: 17000000 INFO @ Mon, 12 Aug 2019 21:55:41: 16000000 INFO @ Mon, 12 Aug 2019 21:55:42: 16000000 INFO @ Mon, 12 Aug 2019 21:55:44: 18000000 INFO @ Mon, 12 Aug 2019 21:55:48: 17000000 INFO @ Mon, 12 Aug 2019 21:55:49: 17000000 INFO @ Mon, 12 Aug 2019 21:55:50: 19000000 INFO @ Mon, 12 Aug 2019 21:55:55: 18000000 INFO @ Mon, 12 Aug 2019 21:55:56: 18000000 INFO @ Mon, 12 Aug 2019 21:55:57: 20000000 INFO @ Mon, 12 Aug 2019 21:56:03: 19000000 INFO @ Mon, 12 Aug 2019 21:56:03: 19000000 INFO @ Mon, 12 Aug 2019 21:56:03: 21000000 INFO @ Mon, 12 Aug 2019 21:56:10: 20000000 INFO @ Mon, 12 Aug 2019 21:56:10: 22000000 INFO @ Mon, 12 Aug 2019 21:56:10: 20000000 INFO @ Mon, 12 Aug 2019 21:56:17: 23000000 INFO @ Mon, 12 Aug 2019 21:56:17: 21000000 INFO @ Mon, 12 Aug 2019 21:56:18: 21000000 INFO @ Mon, 12 Aug 2019 21:56:23: 24000000 INFO @ Mon, 12 Aug 2019 21:56:24: 22000000 INFO @ Mon, 12 Aug 2019 21:56:25: 22000000 INFO @ Mon, 12 Aug 2019 21:56:30: 25000000 INFO @ Mon, 12 Aug 2019 21:56:31: 23000000 INFO @ Mon, 12 Aug 2019 21:56:32: 23000000 INFO @ Mon, 12 Aug 2019 21:56:36: 26000000 INFO @ Mon, 12 Aug 2019 21:56:39: 24000000 INFO @ Mon, 12 Aug 2019 21:56:39: 24000000 INFO @ Mon, 12 Aug 2019 21:56:43: 27000000 INFO @ Mon, 12 Aug 2019 21:56:46: 25000000 INFO @ Mon, 12 Aug 2019 21:56:47: 25000000 INFO @ Mon, 12 Aug 2019 21:56:50: 28000000 INFO @ Mon, 12 Aug 2019 21:56:53: 26000000 INFO @ Mon, 12 Aug 2019 21:56:54: 26000000 INFO @ Mon, 12 Aug 2019 21:56:56: 29000000 INFO @ Mon, 12 Aug 2019 21:57:01: 27000000 INFO @ Mon, 12 Aug 2019 21:57:01: 27000000 INFO @ Mon, 12 Aug 2019 21:57:03: 30000000 INFO @ Mon, 12 Aug 2019 21:57:08: 28000000 INFO @ Mon, 12 Aug 2019 21:57:08: 28000000 INFO @ Mon, 12 Aug 2019 21:57:10: 31000000 INFO @ Mon, 12 Aug 2019 21:57:15: 29000000 INFO @ Mon, 12 Aug 2019 21:57:16: 29000000 INFO @ Mon, 12 Aug 2019 21:57:17: 32000000 INFO @ Mon, 12 Aug 2019 21:57:23: 30000000 INFO @ Mon, 12 Aug 2019 21:57:23: 30000000 INFO @ Mon, 12 Aug 2019 21:57:23: 33000000 INFO @ Mon, 12 Aug 2019 21:57:30: 34000000 INFO @ Mon, 12 Aug 2019 21:57:30: 31000000 INFO @ Mon, 12 Aug 2019 21:57:30: 31000000 INFO @ Mon, 12 Aug 2019 21:57:37: 35000000 INFO @ Mon, 12 Aug 2019 21:57:37: 32000000 INFO @ Mon, 12 Aug 2019 21:57:38: 32000000 INFO @ Mon, 12 Aug 2019 21:57:43: 36000000 INFO @ Mon, 12 Aug 2019 21:57:44: 33000000 INFO @ Mon, 12 Aug 2019 21:57:45: 33000000 INFO @ Mon, 12 Aug 2019 21:57:45: #1 tag size is determined as 51 bps INFO @ Mon, 12 Aug 2019 21:57:45: #1 tag size = 51 INFO @ Mon, 12 Aug 2019 21:57:45: #1 total tags in treatment: 36290983 INFO @ Mon, 12 Aug 2019 21:57:45: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 21:57:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 21:57:46: #1 tags after filtering in treatment: 36290983 INFO @ Mon, 12 Aug 2019 21:57:46: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 21:57:46: #1 finished! INFO @ Mon, 12 Aug 2019 21:57:46: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 21:57:46: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 21:57:49: #2 number of paired peaks: 12 WARNING @ Mon, 12 Aug 2019 21:57:49: Too few paired peaks (12) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 21:57:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981670/SRX3981670.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981670/SRX3981670.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981670/SRX3981670.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981670/SRX3981670.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 21:57:52: 34000000 INFO @ Mon, 12 Aug 2019 21:57:52: 34000000 INFO @ Mon, 12 Aug 2019 21:57:59: 35000000 INFO @ Mon, 12 Aug 2019 21:57:59: 35000000 INFO @ Mon, 12 Aug 2019 21:58:06: 36000000 INFO @ Mon, 12 Aug 2019 21:58:06: 36000000 INFO @ Mon, 12 Aug 2019 21:58:08: #1 tag size is determined as 51 bps INFO @ Mon, 12 Aug 2019 21:58:08: #1 tag size = 51 INFO @ Mon, 12 Aug 2019 21:58:08: #1 total tags in treatment: 36290983 INFO @ Mon, 12 Aug 2019 21:58:08: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 21:58:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 21:58:09: #1 tag size is determined as 51 bps INFO @ Mon, 12 Aug 2019 21:58:09: #1 tag size = 51 INFO @ Mon, 12 Aug 2019 21:58:09: #1 total tags in treatment: 36290983 INFO @ Mon, 12 Aug 2019 21:58:09: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 21:58:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 21:58:09: #1 tags after filtering in treatment: 36290983 INFO @ Mon, 12 Aug 2019 21:58:09: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 21:58:09: #1 finished! INFO @ Mon, 12 Aug 2019 21:58:09: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 21:58:09: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 21:58:10: #1 tags after filtering in treatment: 36290983 INFO @ Mon, 12 Aug 2019 21:58:10: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 21:58:10: #1 finished! INFO @ Mon, 12 Aug 2019 21:58:10: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 21:58:10: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 21:58:12: #2 number of paired peaks: 12 WARNING @ Mon, 12 Aug 2019 21:58:12: Too few paired peaks (12) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 21:58:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981670/SRX3981670.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981670/SRX3981670.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981670/SRX3981670.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981670/SRX3981670.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 21:58:13: #2 number of paired peaks: 12 WARNING @ Mon, 12 Aug 2019 21:58:13: Too few paired peaks (12) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 21:58:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981670/SRX3981670.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981670/SRX3981670.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981670/SRX3981670.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981670/SRX3981670.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。