Job ID = 2590434 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 15,758,532 reads read : 31,517,064 reads written : 15,758,532 reads 0-length : 15,758,532 spots read : 15,726,977 reads read : 31,453,954 reads written : 15,726,977 reads 0-length : 15,726,977 spots read : 15,841,430 reads read : 31,682,860 reads written : 15,841,430 reads 0-length : 15,841,430 fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:16:28 47326939 reads; of these: 47326939 (100.00%) were unpaired; of these: 2293564 (4.85%) aligned 0 times 33471290 (70.72%) aligned exactly 1 time 11562085 (24.43%) aligned >1 times 95.15% overall alignment rate Time searching: 00:16:28 Overall time: 00:16:28 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 5381398 / 45033375 = 0.1195 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 12 Aug 2019 21:56:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981668/SRX3981668.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981668/SRX3981668.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981668/SRX3981668.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981668/SRX3981668.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 21:56:19: #1 read tag files... INFO @ Mon, 12 Aug 2019 21:56:19: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 21:56:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981668/SRX3981668.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981668/SRX3981668.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981668/SRX3981668.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981668/SRX3981668.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 21:56:19: #1 read tag files... INFO @ Mon, 12 Aug 2019 21:56:19: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 21:56:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981668/SRX3981668.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981668/SRX3981668.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981668/SRX3981668.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981668/SRX3981668.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 21:56:21: #1 read tag files... INFO @ Mon, 12 Aug 2019 21:56:21: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 21:56:27: 1000000 INFO @ Mon, 12 Aug 2019 21:56:27: 1000000 INFO @ Mon, 12 Aug 2019 21:56:28: 1000000 INFO @ Mon, 12 Aug 2019 21:56:34: 2000000 INFO @ Mon, 12 Aug 2019 21:56:35: 2000000 INFO @ Mon, 12 Aug 2019 21:56:36: 2000000 INFO @ Mon, 12 Aug 2019 21:56:40: 3000000 INFO @ Mon, 12 Aug 2019 21:56:43: 3000000 INFO @ Mon, 12 Aug 2019 21:56:44: 3000000 INFO @ Mon, 12 Aug 2019 21:56:47: 4000000 INFO @ Mon, 12 Aug 2019 21:56:50: 4000000 INFO @ Mon, 12 Aug 2019 21:56:52: 4000000 INFO @ Mon, 12 Aug 2019 21:56:53: 5000000 INFO @ Mon, 12 Aug 2019 21:56:59: 5000000 INFO @ Mon, 12 Aug 2019 21:56:59: 5000000 INFO @ Mon, 12 Aug 2019 21:57:00: 6000000 INFO @ Mon, 12 Aug 2019 21:57:06: 6000000 INFO @ Mon, 12 Aug 2019 21:57:06: 7000000 INFO @ Mon, 12 Aug 2019 21:57:07: 6000000 INFO @ Mon, 12 Aug 2019 21:57:12: 8000000 INFO @ Mon, 12 Aug 2019 21:57:13: 7000000 INFO @ Mon, 12 Aug 2019 21:57:15: 7000000 INFO @ Mon, 12 Aug 2019 21:57:19: 9000000 INFO @ Mon, 12 Aug 2019 21:57:21: 8000000 INFO @ Mon, 12 Aug 2019 21:57:23: 8000000 INFO @ Mon, 12 Aug 2019 21:57:25: 10000000 INFO @ Mon, 12 Aug 2019 21:57:29: 9000000 INFO @ Mon, 12 Aug 2019 21:57:31: 9000000 INFO @ Mon, 12 Aug 2019 21:57:32: 11000000 INFO @ Mon, 12 Aug 2019 21:57:37: 10000000 INFO @ Mon, 12 Aug 2019 21:57:38: 12000000 INFO @ Mon, 12 Aug 2019 21:57:39: 10000000 INFO @ Mon, 12 Aug 2019 21:57:45: 11000000 INFO @ Mon, 12 Aug 2019 21:57:45: 13000000 INFO @ Mon, 12 Aug 2019 21:57:46: 11000000 INFO @ Mon, 12 Aug 2019 21:57:52: 14000000 INFO @ Mon, 12 Aug 2019 21:57:53: 12000000 INFO @ Mon, 12 Aug 2019 21:57:53: 12000000 INFO @ Mon, 12 Aug 2019 21:57:58: 15000000 INFO @ Mon, 12 Aug 2019 21:58:01: 13000000 INFO @ Mon, 12 Aug 2019 21:58:02: 13000000 INFO @ Mon, 12 Aug 2019 21:58:05: 16000000 INFO @ Mon, 12 Aug 2019 21:58:09: 14000000 INFO @ Mon, 12 Aug 2019 21:58:10: 14000000 INFO @ Mon, 12 Aug 2019 21:58:11: 17000000 INFO @ Mon, 12 Aug 2019 21:58:17: 15000000 INFO @ Mon, 12 Aug 2019 21:58:17: 18000000 INFO @ Mon, 12 Aug 2019 21:58:18: 15000000 INFO @ Mon, 12 Aug 2019 21:58:24: 19000000 INFO @ Mon, 12 Aug 2019 21:58:25: 16000000 INFO @ Mon, 12 Aug 2019 21:58:26: 16000000 INFO @ Mon, 12 Aug 2019 21:58:30: 20000000 INFO @ Mon, 12 Aug 2019 21:58:32: 17000000 INFO @ Mon, 12 Aug 2019 21:58:34: 17000000 INFO @ Mon, 12 Aug 2019 21:58:38: 21000000 INFO @ Mon, 12 Aug 2019 21:58:40: 18000000 INFO @ Mon, 12 Aug 2019 21:58:42: 18000000 INFO @ Mon, 12 Aug 2019 21:58:45: 22000000 INFO @ Mon, 12 Aug 2019 21:58:47: 19000000 INFO @ Mon, 12 Aug 2019 21:58:50: 19000000 INFO @ Mon, 12 Aug 2019 21:58:52: 23000000 INFO @ Mon, 12 Aug 2019 21:58:54: 20000000 INFO @ Mon, 12 Aug 2019 21:58:58: 20000000 INFO @ Mon, 12 Aug 2019 21:58:59: 24000000 INFO @ Mon, 12 Aug 2019 21:59:01: 21000000 INFO @ Mon, 12 Aug 2019 21:59:06: 25000000 INFO @ Mon, 12 Aug 2019 21:59:07: 21000000 INFO @ Mon, 12 Aug 2019 21:59:08: 22000000 INFO @ Mon, 12 Aug 2019 21:59:13: 26000000 INFO @ Mon, 12 Aug 2019 21:59:15: 22000000 INFO @ Mon, 12 Aug 2019 21:59:16: 23000000 INFO @ Mon, 12 Aug 2019 21:59:20: 27000000 INFO @ Mon, 12 Aug 2019 21:59:23: 23000000 INFO @ Mon, 12 Aug 2019 21:59:23: 24000000 INFO @ Mon, 12 Aug 2019 21:59:27: 28000000 INFO @ Mon, 12 Aug 2019 21:59:30: 25000000 INFO @ Mon, 12 Aug 2019 21:59:31: 24000000 INFO @ Mon, 12 Aug 2019 21:59:35: 29000000 INFO @ Mon, 12 Aug 2019 21:59:38: 26000000 INFO @ Mon, 12 Aug 2019 21:59:39: 25000000 INFO @ Mon, 12 Aug 2019 21:59:43: 30000000 INFO @ Mon, 12 Aug 2019 21:59:45: 27000000 INFO @ Mon, 12 Aug 2019 21:59:47: 26000000 INFO @ Mon, 12 Aug 2019 21:59:50: 31000000 INFO @ Mon, 12 Aug 2019 21:59:53: 28000000 INFO @ Mon, 12 Aug 2019 21:59:55: 27000000 INFO @ Mon, 12 Aug 2019 21:59:58: 32000000 INFO @ Mon, 12 Aug 2019 22:00:01: 29000000 INFO @ Mon, 12 Aug 2019 22:00:03: 28000000 INFO @ Mon, 12 Aug 2019 22:00:06: 33000000 INFO @ Mon, 12 Aug 2019 22:00:08: 30000000 INFO @ Mon, 12 Aug 2019 22:00:11: 29000000 INFO @ Mon, 12 Aug 2019 22:00:13: 34000000 INFO @ Mon, 12 Aug 2019 22:00:16: 31000000 INFO @ Mon, 12 Aug 2019 22:00:19: 30000000 INFO @ Mon, 12 Aug 2019 22:00:20: 35000000 INFO @ Mon, 12 Aug 2019 22:00:23: 32000000 INFO @ Mon, 12 Aug 2019 22:00:27: 31000000 INFO @ Mon, 12 Aug 2019 22:00:28: 36000000 INFO @ Mon, 12 Aug 2019 22:00:31: 33000000 INFO @ Mon, 12 Aug 2019 22:00:35: 32000000 INFO @ Mon, 12 Aug 2019 22:00:35: 37000000 INFO @ Mon, 12 Aug 2019 22:00:38: 34000000 INFO @ Mon, 12 Aug 2019 22:00:43: 38000000 INFO @ Mon, 12 Aug 2019 22:00:43: 33000000 INFO @ Mon, 12 Aug 2019 22:00:45: 35000000 INFO @ Mon, 12 Aug 2019 22:00:50: 39000000 INFO @ Mon, 12 Aug 2019 22:00:51: 34000000 INFO @ Mon, 12 Aug 2019 22:00:53: 36000000 INFO @ Mon, 12 Aug 2019 22:00:56: #1 tag size is determined as 51 bps INFO @ Mon, 12 Aug 2019 22:00:56: #1 tag size = 51 INFO @ Mon, 12 Aug 2019 22:00:56: #1 total tags in treatment: 39651977 INFO @ Mon, 12 Aug 2019 22:00:56: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 22:00:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 22:00:56: #1 tags after filtering in treatment: 39651977 INFO @ Mon, 12 Aug 2019 22:00:56: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 22:00:56: #1 finished! INFO @ Mon, 12 Aug 2019 22:00:56: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 22:00:56: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 22:00:59: 35000000 INFO @ Mon, 12 Aug 2019 22:01:00: #2 number of paired peaks: 0 WARNING @ Mon, 12 Aug 2019 22:01:00: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 22:01:00: Process for pairing-model is terminated! INFO @ Mon, 12 Aug 2019 22:01:00: 37000000 cut: /home/okishinya/chipatlas/results/dm3/SRX3981668/SRX3981668.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981668/SRX3981668.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981668/SRX3981668.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981668/SRX3981668.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 22:01:06: 38000000 INFO @ Mon, 12 Aug 2019 22:01:07: 36000000 INFO @ Mon, 12 Aug 2019 22:01:13: 39000000 INFO @ Mon, 12 Aug 2019 22:01:15: 37000000 INFO @ Mon, 12 Aug 2019 22:01:17: #1 tag size is determined as 51 bps INFO @ Mon, 12 Aug 2019 22:01:17: #1 tag size = 51 INFO @ Mon, 12 Aug 2019 22:01:17: #1 total tags in treatment: 39651977 INFO @ Mon, 12 Aug 2019 22:01:17: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 22:01:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 22:01:18: #1 tags after filtering in treatment: 39651977 INFO @ Mon, 12 Aug 2019 22:01:18: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 22:01:18: #1 finished! INFO @ Mon, 12 Aug 2019 22:01:18: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 22:01:18: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 22:01:21: #2 number of paired peaks: 0 WARNING @ Mon, 12 Aug 2019 22:01:21: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 22:01:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981668/SRX3981668.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981668/SRX3981668.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981668/SRX3981668.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981668/SRX3981668.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 22:01:23: 38000000 INFO @ Mon, 12 Aug 2019 22:01:31: 39000000 INFO @ Mon, 12 Aug 2019 22:01:36: #1 tag size is determined as 51 bps INFO @ Mon, 12 Aug 2019 22:01:36: #1 tag size = 51 INFO @ Mon, 12 Aug 2019 22:01:36: #1 total tags in treatment: 39651977 INFO @ Mon, 12 Aug 2019 22:01:36: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 22:01:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 22:01:37: #1 tags after filtering in treatment: 39651977 INFO @ Mon, 12 Aug 2019 22:01:37: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 22:01:37: #1 finished! INFO @ Mon, 12 Aug 2019 22:01:37: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 22:01:37: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 22:01:40: #2 number of paired peaks: 0 WARNING @ Mon, 12 Aug 2019 22:01:40: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 22:01:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981668/SRX3981668.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981668/SRX3981668.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981668/SRX3981668.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981668/SRX3981668.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。