Job ID = 6528049 SRX = SRX3981666 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:14:21 prefetch.2.10.7: 1) Downloading 'SRR7050382'... 2020-06-29T14:14:21 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:15:16 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:15:16 prefetch.2.10.7: 'SRR7050382' is valid 2020-06-29T14:15:16 prefetch.2.10.7: 1) 'SRR7050382' was downloaded successfully 2020-06-29T14:15:16 prefetch.2.10.7: 'SRR7050382' has 0 unresolved dependencies Read 12282915 spots for SRR7050382/SRR7050382.sra Written 12282915 spots for SRR7050382/SRR7050382.sra 2020-06-29T14:16:11 prefetch.2.10.7: 1) Downloading 'SRR7050383'... 2020-06-29T14:16:11 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:17:11 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:17:12 prefetch.2.10.7: 'SRR7050383' is valid 2020-06-29T14:17:12 prefetch.2.10.7: 1) 'SRR7050383' was downloaded successfully 2020-06-29T14:17:12 prefetch.2.10.7: 'SRR7050383' has 0 unresolved dependencies Read 12260825 spots for SRR7050383/SRR7050383.sra Written 12260825 spots for SRR7050383/SRR7050383.sra 2020-06-29T14:18:05 prefetch.2.10.7: 1) Downloading 'SRR7050384'... 2020-06-29T14:18:05 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:18:55 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:18:55 prefetch.2.10.7: 'SRR7050384' is valid 2020-06-29T14:18:55 prefetch.2.10.7: 1) 'SRR7050384' was downloaded successfully 2020-06-29T14:18:55 prefetch.2.10.7: 'SRR7050384' has 0 unresolved dependencies Read 12342585 spots for SRR7050384/SRR7050384.sra Written 12342585 spots for SRR7050384/SRR7050384.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:30 36886325 reads; of these: 36886325 (100.00%) were unpaired; of these: 1200281 (3.25%) aligned 0 times 26404901 (71.58%) aligned exactly 1 time 9281143 (25.16%) aligned >1 times 96.75% overall alignment rate Time searching: 00:11:30 Overall time: 00:11:30 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 3823521 / 35686044 = 0.1071 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:47:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981666/SRX3981666.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981666/SRX3981666.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981666/SRX3981666.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981666/SRX3981666.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:47:26: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:47:26: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:47:31: 1000000 INFO @ Mon, 29 Jun 2020 23:47:36: 2000000 INFO @ Mon, 29 Jun 2020 23:47:42: 3000000 INFO @ Mon, 29 Jun 2020 23:47:47: 4000000 INFO @ Mon, 29 Jun 2020 23:47:52: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:47:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981666/SRX3981666.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981666/SRX3981666.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981666/SRX3981666.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981666/SRX3981666.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:47:56: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:47:56: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:47:57: 6000000 INFO @ Mon, 29 Jun 2020 23:48:02: 1000000 INFO @ Mon, 29 Jun 2020 23:48:03: 7000000 INFO @ Mon, 29 Jun 2020 23:48:07: 2000000 INFO @ Mon, 29 Jun 2020 23:48:08: 8000000 INFO @ Mon, 29 Jun 2020 23:48:13: 3000000 INFO @ Mon, 29 Jun 2020 23:48:13: 9000000 INFO @ Mon, 29 Jun 2020 23:48:19: 4000000 INFO @ Mon, 29 Jun 2020 23:48:19: 10000000 BedGraph に変換中... INFO @ Mon, 29 Jun 2020 23:48:24: 5000000 INFO @ Mon, 29 Jun 2020 23:48:24: 11000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:48:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981666/SRX3981666.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981666/SRX3981666.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981666/SRX3981666.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981666/SRX3981666.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:48:26: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:48:26: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:48:30: 12000000 INFO @ Mon, 29 Jun 2020 23:48:30: 6000000 INFO @ Mon, 29 Jun 2020 23:48:32: 1000000 INFO @ Mon, 29 Jun 2020 23:48:35: 13000000 INFO @ Mon, 29 Jun 2020 23:48:36: 7000000 INFO @ Mon, 29 Jun 2020 23:48:38: 2000000 INFO @ Mon, 29 Jun 2020 23:48:41: 14000000 INFO @ Mon, 29 Jun 2020 23:48:41: 8000000 INFO @ Mon, 29 Jun 2020 23:48:43: 3000000 INFO @ Mon, 29 Jun 2020 23:48:47: 15000000 INFO @ Mon, 29 Jun 2020 23:48:47: 9000000 INFO @ Mon, 29 Jun 2020 23:48:49: 4000000 INFO @ Mon, 29 Jun 2020 23:48:52: 16000000 INFO @ Mon, 29 Jun 2020 23:48:52: 10000000 INFO @ Mon, 29 Jun 2020 23:48:54: 5000000 INFO @ Mon, 29 Jun 2020 23:48:58: 17000000 INFO @ Mon, 29 Jun 2020 23:48:58: 11000000 INFO @ Mon, 29 Jun 2020 23:49:00: 6000000 INFO @ Mon, 29 Jun 2020 23:49:03: 18000000 INFO @ Mon, 29 Jun 2020 23:49:04: 12000000 INFO @ Mon, 29 Jun 2020 23:49:05: 7000000 INFO @ Mon, 29 Jun 2020 23:49:08: 19000000 INFO @ Mon, 29 Jun 2020 23:49:09: 13000000 INFO @ Mon, 29 Jun 2020 23:49:11: 8000000 INFO @ Mon, 29 Jun 2020 23:49:14: 20000000 INFO @ Mon, 29 Jun 2020 23:49:15: 14000000 INFO @ Mon, 29 Jun 2020 23:49:16: 9000000 INFO @ Mon, 29 Jun 2020 23:49:19: 21000000 INFO @ Mon, 29 Jun 2020 23:49:20: 15000000 INFO @ Mon, 29 Jun 2020 23:49:22: 10000000 INFO @ Mon, 29 Jun 2020 23:49:24: 22000000 INFO @ Mon, 29 Jun 2020 23:49:26: 16000000 INFO @ Mon, 29 Jun 2020 23:49:27: 11000000 INFO @ Mon, 29 Jun 2020 23:49:30: 23000000 INFO @ Mon, 29 Jun 2020 23:49:31: 17000000 INFO @ Mon, 29 Jun 2020 23:49:33: 12000000 INFO @ Mon, 29 Jun 2020 23:49:35: 24000000 INFO @ Mon, 29 Jun 2020 23:49:37: 18000000 INFO @ Mon, 29 Jun 2020 23:49:38: 13000000 INFO @ Mon, 29 Jun 2020 23:49:40: 25000000 INFO @ Mon, 29 Jun 2020 23:49:42: 19000000 INFO @ Mon, 29 Jun 2020 23:49:43: 14000000 INFO @ Mon, 29 Jun 2020 23:49:45: 26000000 INFO @ Mon, 29 Jun 2020 23:49:48: 20000000 INFO @ Mon, 29 Jun 2020 23:49:49: 15000000 INFO @ Mon, 29 Jun 2020 23:49:51: 27000000 INFO @ Mon, 29 Jun 2020 23:49:53: 21000000 INFO @ Mon, 29 Jun 2020 23:49:54: 16000000 INFO @ Mon, 29 Jun 2020 23:49:56: 28000000 INFO @ Mon, 29 Jun 2020 23:49:58: 22000000 INFO @ Mon, 29 Jun 2020 23:50:00: 17000000 INFO @ Mon, 29 Jun 2020 23:50:02: 29000000 INFO @ Mon, 29 Jun 2020 23:50:03: 23000000 INFO @ Mon, 29 Jun 2020 23:50:05: 18000000 INFO @ Mon, 29 Jun 2020 23:50:07: 30000000 INFO @ Mon, 29 Jun 2020 23:50:09: 24000000 INFO @ Mon, 29 Jun 2020 23:50:10: 19000000 INFO @ Mon, 29 Jun 2020 23:50:12: 31000000 INFO @ Mon, 29 Jun 2020 23:50:14: 25000000 INFO @ Mon, 29 Jun 2020 23:50:15: 20000000 INFO @ Mon, 29 Jun 2020 23:50:17: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:50:17: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:50:17: #1 total tags in treatment: 31862523 INFO @ Mon, 29 Jun 2020 23:50:17: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:50:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:50:18: #1 tags after filtering in treatment: 31862523 INFO @ Mon, 29 Jun 2020 23:50:18: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:50:18: #1 finished! INFO @ Mon, 29 Jun 2020 23:50:18: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:50:18: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:50:19: 26000000 INFO @ Mon, 29 Jun 2020 23:50:20: #2 number of paired peaks: 22 WARNING @ Mon, 29 Jun 2020 23:50:20: Too few paired peaks (22) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:50:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981666/SRX3981666.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981666/SRX3981666.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981666/SRX3981666.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981666/SRX3981666.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:50:20: 21000000 INFO @ Mon, 29 Jun 2020 23:50:24: 27000000 INFO @ Mon, 29 Jun 2020 23:50:26: 22000000 INFO @ Mon, 29 Jun 2020 23:50:30: 28000000 INFO @ Mon, 29 Jun 2020 23:50:31: 23000000 INFO @ Mon, 29 Jun 2020 23:50:35: 29000000 INFO @ Mon, 29 Jun 2020 23:50:36: 24000000 INFO @ Mon, 29 Jun 2020 23:50:40: 30000000 INFO @ Mon, 29 Jun 2020 23:50:41: 25000000 INFO @ Mon, 29 Jun 2020 23:50:46: 31000000 INFO @ Mon, 29 Jun 2020 23:50:46: 26000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 23:50:51: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:50:51: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:50:51: #1 total tags in treatment: 31862523 INFO @ Mon, 29 Jun 2020 23:50:51: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:50:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:50:51: #1 tags after filtering in treatment: 31862523 INFO @ Mon, 29 Jun 2020 23:50:51: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:50:51: #1 finished! INFO @ Mon, 29 Jun 2020 23:50:51: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:50:51: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:50:52: 27000000 INFO @ Mon, 29 Jun 2020 23:50:53: #2 number of paired peaks: 22 WARNING @ Mon, 29 Jun 2020 23:50:53: Too few paired peaks (22) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:50:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981666/SRX3981666.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981666/SRX3981666.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981666/SRX3981666.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981666/SRX3981666.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:50:57: 28000000 INFO @ Mon, 29 Jun 2020 23:51:02: 29000000 INFO @ Mon, 29 Jun 2020 23:51:08: 30000000 INFO @ Mon, 29 Jun 2020 23:51:13: 31000000 INFO @ Mon, 29 Jun 2020 23:51:18: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:51:18: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:51:18: #1 total tags in treatment: 31862523 INFO @ Mon, 29 Jun 2020 23:51:18: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:51:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:51:18: #1 tags after filtering in treatment: 31862523 INFO @ Mon, 29 Jun 2020 23:51:18: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:51:18: #1 finished! INFO @ Mon, 29 Jun 2020 23:51:18: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:51:18: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:51:20: #2 number of paired peaks: 22 WARNING @ Mon, 29 Jun 2020 23:51:20: Too few paired peaks (22) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:51:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981666/SRX3981666.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981666/SRX3981666.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981666/SRX3981666.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981666/SRX3981666.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。