Job ID = 6528047 SRX = SRX3981665 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:38:58 prefetch.2.10.7: 1) Downloading 'SRR7050379'... 2020-06-29T14:38:58 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:40:35 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:40:35 prefetch.2.10.7: 'SRR7050379' is valid 2020-06-29T14:40:35 prefetch.2.10.7: 1) 'SRR7050379' was downloaded successfully 2020-06-29T14:40:35 prefetch.2.10.7: 'SRR7050379' has 0 unresolved dependencies Read 12334069 spots for SRR7050379/SRR7050379.sra Written 12334069 spots for SRR7050379/SRR7050379.sra 2020-06-29T14:41:36 prefetch.2.10.7: 1) Downloading 'SRR7050380'... 2020-06-29T14:41:36 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:42:35 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:42:36 prefetch.2.10.7: 'SRR7050380' is valid 2020-06-29T14:42:36 prefetch.2.10.7: 1) 'SRR7050380' was downloaded successfully 2020-06-29T14:42:36 prefetch.2.10.7: 'SRR7050380' has 0 unresolved dependencies Read 12319768 spots for SRR7050380/SRR7050380.sra Written 12319768 spots for SRR7050380/SRR7050380.sra 2020-06-29T14:43:36 prefetch.2.10.7: 1) Downloading 'SRR7050381'... 2020-06-29T14:43:36 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:44:38 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:44:39 prefetch.2.10.7: 'SRR7050381' is valid 2020-06-29T14:44:39 prefetch.2.10.7: 1) 'SRR7050381' was downloaded successfully 2020-06-29T14:44:39 prefetch.2.10.7: 'SRR7050381' has 0 unresolved dependencies Read 12416584 spots for SRR7050381/SRR7050381.sra Written 12416584 spots for SRR7050381/SRR7050381.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:12:06 37070421 reads; of these: 37070421 (100.00%) were unpaired; of these: 1339946 (3.61%) aligned 0 times 26545311 (71.61%) aligned exactly 1 time 9185164 (24.78%) aligned >1 times 96.39% overall alignment rate Time searching: 00:12:06 Overall time: 00:12:06 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 3709803 / 35730475 = 0.1038 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:18:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981665/SRX3981665.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981665/SRX3981665.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981665/SRX3981665.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981665/SRX3981665.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:18:20: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:18:20: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:18:25: 1000000 INFO @ Tue, 30 Jun 2020 00:18:30: 2000000 INFO @ Tue, 30 Jun 2020 00:18:36: 3000000 INFO @ Tue, 30 Jun 2020 00:18:41: 4000000 INFO @ Tue, 30 Jun 2020 00:18:46: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:18:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981665/SRX3981665.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981665/SRX3981665.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981665/SRX3981665.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981665/SRX3981665.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:18:50: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:18:50: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:18:51: 6000000 INFO @ Tue, 30 Jun 2020 00:18:55: 1000000 INFO @ Tue, 30 Jun 2020 00:18:56: 7000000 INFO @ Tue, 30 Jun 2020 00:19:00: 2000000 INFO @ Tue, 30 Jun 2020 00:19:01: 8000000 INFO @ Tue, 30 Jun 2020 00:19:05: 3000000 INFO @ Tue, 30 Jun 2020 00:19:06: 9000000 INFO @ Tue, 30 Jun 2020 00:19:10: 4000000 INFO @ Tue, 30 Jun 2020 00:19:11: 10000000 INFO @ Tue, 30 Jun 2020 00:19:15: 5000000 INFO @ Tue, 30 Jun 2020 00:19:15: 11000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:19:19: 6000000 INFO @ Tue, 30 Jun 2020 00:19:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981665/SRX3981665.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981665/SRX3981665.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981665/SRX3981665.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981665/SRX3981665.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:19:20: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:19:20: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:19:20: 12000000 INFO @ Tue, 30 Jun 2020 00:19:24: 7000000 INFO @ Tue, 30 Jun 2020 00:19:25: 1000000 INFO @ Tue, 30 Jun 2020 00:19:26: 13000000 INFO @ Tue, 30 Jun 2020 00:19:29: 8000000 INFO @ Tue, 30 Jun 2020 00:19:31: 2000000 INFO @ Tue, 30 Jun 2020 00:19:31: 14000000 INFO @ Tue, 30 Jun 2020 00:19:34: 9000000 INFO @ Tue, 30 Jun 2020 00:19:36: 3000000 INFO @ Tue, 30 Jun 2020 00:19:36: 15000000 INFO @ Tue, 30 Jun 2020 00:19:39: 10000000 INFO @ Tue, 30 Jun 2020 00:19:41: 4000000 INFO @ Tue, 30 Jun 2020 00:19:42: 16000000 INFO @ Tue, 30 Jun 2020 00:19:44: 11000000 INFO @ Tue, 30 Jun 2020 00:19:47: 5000000 INFO @ Tue, 30 Jun 2020 00:19:47: 17000000 INFO @ Tue, 30 Jun 2020 00:19:49: 12000000 INFO @ Tue, 30 Jun 2020 00:19:52: 6000000 INFO @ Tue, 30 Jun 2020 00:19:52: 18000000 INFO @ Tue, 30 Jun 2020 00:19:54: 13000000 INFO @ Tue, 30 Jun 2020 00:19:57: 7000000 INFO @ Tue, 30 Jun 2020 00:19:58: 19000000 INFO @ Tue, 30 Jun 2020 00:19:59: 14000000 INFO @ Tue, 30 Jun 2020 00:20:03: 8000000 INFO @ Tue, 30 Jun 2020 00:20:03: 20000000 INFO @ Tue, 30 Jun 2020 00:20:04: 15000000 INFO @ Tue, 30 Jun 2020 00:20:08: 9000000 INFO @ Tue, 30 Jun 2020 00:20:08: 21000000 INFO @ Tue, 30 Jun 2020 00:20:09: 16000000 INFO @ Tue, 30 Jun 2020 00:20:14: 10000000 INFO @ Tue, 30 Jun 2020 00:20:14: 22000000 INFO @ Tue, 30 Jun 2020 00:20:14: 17000000 INFO @ Tue, 30 Jun 2020 00:20:19: 11000000 INFO @ Tue, 30 Jun 2020 00:20:19: 23000000 INFO @ Tue, 30 Jun 2020 00:20:19: 18000000 INFO @ Tue, 30 Jun 2020 00:20:24: 12000000 INFO @ Tue, 30 Jun 2020 00:20:24: 19000000 INFO @ Tue, 30 Jun 2020 00:20:24: 24000000 INFO @ Tue, 30 Jun 2020 00:20:29: 20000000 INFO @ Tue, 30 Jun 2020 00:20:29: 13000000 INFO @ Tue, 30 Jun 2020 00:20:29: 25000000 INFO @ Tue, 30 Jun 2020 00:20:34: 21000000 INFO @ Tue, 30 Jun 2020 00:20:35: 14000000 INFO @ Tue, 30 Jun 2020 00:20:35: 26000000 INFO @ Tue, 30 Jun 2020 00:20:39: 22000000 INFO @ Tue, 30 Jun 2020 00:20:40: 15000000 INFO @ Tue, 30 Jun 2020 00:20:40: 27000000 INFO @ Tue, 30 Jun 2020 00:20:44: 23000000 INFO @ Tue, 30 Jun 2020 00:20:45: 16000000 INFO @ Tue, 30 Jun 2020 00:20:45: 28000000 INFO @ Tue, 30 Jun 2020 00:20:49: 24000000 INFO @ Tue, 30 Jun 2020 00:20:51: 17000000 INFO @ Tue, 30 Jun 2020 00:20:51: 29000000 INFO @ Tue, 30 Jun 2020 00:20:53: 25000000 INFO @ Tue, 30 Jun 2020 00:20:56: 18000000 INFO @ Tue, 30 Jun 2020 00:20:56: 30000000 INFO @ Tue, 30 Jun 2020 00:20:58: 26000000 INFO @ Tue, 30 Jun 2020 00:21:01: 19000000 INFO @ Tue, 30 Jun 2020 00:21:02: 31000000 INFO @ Tue, 30 Jun 2020 00:21:03: 27000000 INFO @ Tue, 30 Jun 2020 00:21:06: 20000000 INFO @ Tue, 30 Jun 2020 00:21:07: 32000000 INFO @ Tue, 30 Jun 2020 00:21:08: #1 tag size is determined as 51 bps INFO @ Tue, 30 Jun 2020 00:21:08: #1 tag size = 51 INFO @ Tue, 30 Jun 2020 00:21:08: #1 total tags in treatment: 32020672 INFO @ Tue, 30 Jun 2020 00:21:08: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:21:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:21:08: 28000000 INFO @ Tue, 30 Jun 2020 00:21:08: #1 tags after filtering in treatment: 32020672 INFO @ Tue, 30 Jun 2020 00:21:08: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:21:08: #1 finished! INFO @ Tue, 30 Jun 2020 00:21:08: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:21:08: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:21:10: #2 number of paired peaks: 17 WARNING @ Tue, 30 Jun 2020 00:21:10: Too few paired peaks (17) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:21:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981665/SRX3981665.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981665/SRX3981665.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981665/SRX3981665.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981665/SRX3981665.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:21:11: 21000000 INFO @ Tue, 30 Jun 2020 00:21:13: 29000000 INFO @ Tue, 30 Jun 2020 00:21:16: 22000000 INFO @ Tue, 30 Jun 2020 00:21:18: 30000000 INFO @ Tue, 30 Jun 2020 00:21:21: 23000000 INFO @ Tue, 30 Jun 2020 00:21:23: 31000000 INFO @ Tue, 30 Jun 2020 00:21:26: 24000000 INFO @ Tue, 30 Jun 2020 00:21:28: 32000000 INFO @ Tue, 30 Jun 2020 00:21:28: #1 tag size is determined as 51 bps INFO @ Tue, 30 Jun 2020 00:21:28: #1 tag size = 51 INFO @ Tue, 30 Jun 2020 00:21:28: #1 total tags in treatment: 32020672 INFO @ Tue, 30 Jun 2020 00:21:28: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:21:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:21:29: #1 tags after filtering in treatment: 32020672 INFO @ Tue, 30 Jun 2020 00:21:29: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:21:29: #1 finished! INFO @ Tue, 30 Jun 2020 00:21:29: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:21:29: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:21:31: 25000000 INFO @ Tue, 30 Jun 2020 00:21:31: #2 number of paired peaks: 17 WARNING @ Tue, 30 Jun 2020 00:21:31: Too few paired peaks (17) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:21:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981665/SRX3981665.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981665/SRX3981665.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981665/SRX3981665.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981665/SRX3981665.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:21:35: 26000000 INFO @ Tue, 30 Jun 2020 00:21:40: 27000000 INFO @ Tue, 30 Jun 2020 00:21:45: 28000000 INFO @ Tue, 30 Jun 2020 00:21:50: 29000000 INFO @ Tue, 30 Jun 2020 00:21:54: 30000000 INFO @ Tue, 30 Jun 2020 00:21:59: 31000000 INFO @ Tue, 30 Jun 2020 00:22:04: 32000000 INFO @ Tue, 30 Jun 2020 00:22:05: #1 tag size is determined as 51 bps INFO @ Tue, 30 Jun 2020 00:22:05: #1 tag size = 51 INFO @ Tue, 30 Jun 2020 00:22:05: #1 total tags in treatment: 32020672 INFO @ Tue, 30 Jun 2020 00:22:05: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:22:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:22:05: #1 tags after filtering in treatment: 32020672 INFO @ Tue, 30 Jun 2020 00:22:05: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:22:05: #1 finished! INFO @ Tue, 30 Jun 2020 00:22:05: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:22:05: #2 looking for paired plus/minus strand peaks... BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 00:22:07: #2 number of paired peaks: 17 WARNING @ Tue, 30 Jun 2020 00:22:07: Too few paired peaks (17) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:22:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981665/SRX3981665.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981665/SRX3981665.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981665/SRX3981665.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981665/SRX3981665.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。