Job ID = 2590386 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 11,404,344 reads read : 22,808,688 reads written : 11,404,344 reads 0-length : 11,404,344 spots read : 11,369,091 reads read : 22,738,182 reads written : 11,369,091 reads 0-length : 11,369,091 spots read : 11,437,414 reads read : 22,874,828 reads written : 11,437,414 reads 0-length : 11,437,414 fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:12:02 34210849 reads; of these: 34210849 (100.00%) were unpaired; of these: 1032113 (3.02%) aligned 0 times 24288679 (71.00%) aligned exactly 1 time 8890057 (25.99%) aligned >1 times 96.98% overall alignment rate Time searching: 00:12:02 Overall time: 00:12:02 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 3413985 / 33178736 = 0.1029 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 12 Aug 2019 21:39:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981655/SRX3981655.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981655/SRX3981655.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981655/SRX3981655.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981655/SRX3981655.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 21:39:31: #1 read tag files... INFO @ Mon, 12 Aug 2019 21:39:31: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 21:39:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981655/SRX3981655.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981655/SRX3981655.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981655/SRX3981655.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981655/SRX3981655.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 21:39:32: #1 read tag files... INFO @ Mon, 12 Aug 2019 21:39:32: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 21:39:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981655/SRX3981655.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981655/SRX3981655.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981655/SRX3981655.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981655/SRX3981655.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 21:39:33: #1 read tag files... INFO @ Mon, 12 Aug 2019 21:39:33: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 21:39:38: 1000000 INFO @ Mon, 12 Aug 2019 21:39:38: 1000000 INFO @ Mon, 12 Aug 2019 21:39:40: 1000000 INFO @ Mon, 12 Aug 2019 21:39:45: 2000000 INFO @ Mon, 12 Aug 2019 21:39:45: 2000000 INFO @ Mon, 12 Aug 2019 21:39:46: 2000000 INFO @ Mon, 12 Aug 2019 21:39:51: 3000000 INFO @ Mon, 12 Aug 2019 21:39:51: 3000000 INFO @ Mon, 12 Aug 2019 21:39:53: 3000000 INFO @ Mon, 12 Aug 2019 21:39:57: 4000000 INFO @ Mon, 12 Aug 2019 21:39:58: 4000000 INFO @ Mon, 12 Aug 2019 21:39:59: 4000000 INFO @ Mon, 12 Aug 2019 21:40:03: 5000000 INFO @ Mon, 12 Aug 2019 21:40:04: 5000000 INFO @ Mon, 12 Aug 2019 21:40:06: 5000000 INFO @ Mon, 12 Aug 2019 21:40:09: 6000000 INFO @ Mon, 12 Aug 2019 21:40:11: 6000000 INFO @ Mon, 12 Aug 2019 21:40:12: 6000000 INFO @ Mon, 12 Aug 2019 21:40:15: 7000000 INFO @ Mon, 12 Aug 2019 21:40:17: 7000000 INFO @ Mon, 12 Aug 2019 21:40:19: 7000000 INFO @ Mon, 12 Aug 2019 21:40:21: 8000000 INFO @ Mon, 12 Aug 2019 21:40:24: 8000000 INFO @ Mon, 12 Aug 2019 21:40:25: 8000000 INFO @ Mon, 12 Aug 2019 21:40:27: 9000000 INFO @ Mon, 12 Aug 2019 21:40:30: 9000000 INFO @ Mon, 12 Aug 2019 21:40:32: 9000000 INFO @ Mon, 12 Aug 2019 21:40:33: 10000000 INFO @ Mon, 12 Aug 2019 21:40:37: 10000000 INFO @ Mon, 12 Aug 2019 21:40:38: 10000000 INFO @ Mon, 12 Aug 2019 21:40:39: 11000000 INFO @ Mon, 12 Aug 2019 21:40:43: 11000000 INFO @ Mon, 12 Aug 2019 21:40:45: 11000000 INFO @ Mon, 12 Aug 2019 21:40:45: 12000000 INFO @ Mon, 12 Aug 2019 21:40:50: 12000000 INFO @ Mon, 12 Aug 2019 21:40:51: 12000000 INFO @ Mon, 12 Aug 2019 21:40:51: 13000000 INFO @ Mon, 12 Aug 2019 21:40:56: 13000000 INFO @ Mon, 12 Aug 2019 21:40:57: 14000000 INFO @ Mon, 12 Aug 2019 21:40:58: 13000000 INFO @ Mon, 12 Aug 2019 21:41:03: 14000000 INFO @ Mon, 12 Aug 2019 21:41:04: 15000000 INFO @ Mon, 12 Aug 2019 21:41:04: 14000000 INFO @ Mon, 12 Aug 2019 21:41:09: 15000000 INFO @ Mon, 12 Aug 2019 21:41:10: 16000000 INFO @ Mon, 12 Aug 2019 21:41:11: 15000000 INFO @ Mon, 12 Aug 2019 21:41:16: 17000000 INFO @ Mon, 12 Aug 2019 21:41:16: 16000000 INFO @ Mon, 12 Aug 2019 21:41:17: 16000000 INFO @ Mon, 12 Aug 2019 21:41:22: 18000000 INFO @ Mon, 12 Aug 2019 21:41:22: 17000000 INFO @ Mon, 12 Aug 2019 21:41:24: 17000000 INFO @ Mon, 12 Aug 2019 21:41:28: 19000000 INFO @ Mon, 12 Aug 2019 21:41:29: 18000000 INFO @ Mon, 12 Aug 2019 21:41:30: 18000000 INFO @ Mon, 12 Aug 2019 21:41:34: 20000000 INFO @ Mon, 12 Aug 2019 21:41:35: 19000000 INFO @ Mon, 12 Aug 2019 21:41:37: 19000000 INFO @ Mon, 12 Aug 2019 21:41:40: 21000000 INFO @ Mon, 12 Aug 2019 21:41:42: 20000000 INFO @ Mon, 12 Aug 2019 21:41:43: 20000000 INFO @ Mon, 12 Aug 2019 21:41:46: 22000000 INFO @ Mon, 12 Aug 2019 21:41:48: 21000000 INFO @ Mon, 12 Aug 2019 21:41:50: 21000000 INFO @ Mon, 12 Aug 2019 21:41:52: 23000000 INFO @ Mon, 12 Aug 2019 21:41:54: 22000000 INFO @ Mon, 12 Aug 2019 21:41:56: 22000000 INFO @ Mon, 12 Aug 2019 21:41:58: 24000000 INFO @ Mon, 12 Aug 2019 21:42:01: 23000000 INFO @ Mon, 12 Aug 2019 21:42:03: 23000000 INFO @ Mon, 12 Aug 2019 21:42:04: 25000000 INFO @ Mon, 12 Aug 2019 21:42:07: 24000000 INFO @ Mon, 12 Aug 2019 21:42:09: 24000000 INFO @ Mon, 12 Aug 2019 21:42:10: 26000000 INFO @ Mon, 12 Aug 2019 21:42:14: 25000000 INFO @ Mon, 12 Aug 2019 21:42:16: 25000000 INFO @ Mon, 12 Aug 2019 21:42:16: 27000000 INFO @ Mon, 12 Aug 2019 21:42:20: 26000000 INFO @ Mon, 12 Aug 2019 21:42:22: 28000000 INFO @ Mon, 12 Aug 2019 21:42:22: 26000000 INFO @ Mon, 12 Aug 2019 21:42:27: 27000000 INFO @ Mon, 12 Aug 2019 21:42:28: 29000000 INFO @ Mon, 12 Aug 2019 21:42:29: 27000000 INFO @ Mon, 12 Aug 2019 21:42:33: #1 tag size is determined as 51 bps INFO @ Mon, 12 Aug 2019 21:42:33: #1 tag size = 51 INFO @ Mon, 12 Aug 2019 21:42:33: #1 total tags in treatment: 29764751 INFO @ Mon, 12 Aug 2019 21:42:33: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 21:42:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 21:42:33: 28000000 INFO @ Mon, 12 Aug 2019 21:42:34: #1 tags after filtering in treatment: 29764751 INFO @ Mon, 12 Aug 2019 21:42:34: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 21:42:34: #1 finished! INFO @ Mon, 12 Aug 2019 21:42:34: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 21:42:34: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 21:42:35: 28000000 INFO @ Mon, 12 Aug 2019 21:42:36: #2 number of paired peaks: 41 WARNING @ Mon, 12 Aug 2019 21:42:36: Too few paired peaks (41) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 21:42:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981655/SRX3981655.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981655/SRX3981655.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981655/SRX3981655.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981655/SRX3981655.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 21:42:40: 29000000 INFO @ Mon, 12 Aug 2019 21:42:41: 29000000 INFO @ Mon, 12 Aug 2019 21:42:45: #1 tag size is determined as 51 bps INFO @ Mon, 12 Aug 2019 21:42:45: #1 tag size = 51 INFO @ Mon, 12 Aug 2019 21:42:45: #1 total tags in treatment: 29764751 INFO @ Mon, 12 Aug 2019 21:42:45: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 21:42:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 21:42:46: #1 tags after filtering in treatment: 29764751 INFO @ Mon, 12 Aug 2019 21:42:46: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 21:42:46: #1 finished! INFO @ Mon, 12 Aug 2019 21:42:46: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 21:42:46: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 21:42:47: #1 tag size is determined as 51 bps INFO @ Mon, 12 Aug 2019 21:42:47: #1 tag size = 51 INFO @ Mon, 12 Aug 2019 21:42:47: #1 total tags in treatment: 29764751 INFO @ Mon, 12 Aug 2019 21:42:47: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 21:42:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 21:42:47: #1 tags after filtering in treatment: 29764751 INFO @ Mon, 12 Aug 2019 21:42:47: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 21:42:47: #1 finished! INFO @ Mon, 12 Aug 2019 21:42:47: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 21:42:47: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 21:42:48: #2 number of paired peaks: 41 WARNING @ Mon, 12 Aug 2019 21:42:48: Too few paired peaks (41) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 21:42:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981655/SRX3981655.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981655/SRX3981655.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981655/SRX3981655.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981655/SRX3981655.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 21:42:50: #2 number of paired peaks: 41 WARNING @ Mon, 12 Aug 2019 21:42:50: Too few paired peaks (41) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 21:42:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981655/SRX3981655.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981655/SRX3981655.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981655/SRX3981655.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981655/SRX3981655.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。