Job ID = 6528036 SRX = SRX3981654 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:28:06 prefetch.2.10.7: 1) Downloading 'SRR7050346'... 2020-06-29T14:28:06 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:29:16 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:29:17 prefetch.2.10.7: 'SRR7050346' is valid 2020-06-29T14:29:17 prefetch.2.10.7: 1) 'SRR7050346' was downloaded successfully 2020-06-29T14:29:17 prefetch.2.10.7: 'SRR7050346' has 0 unresolved dependencies Read 12935491 spots for SRR7050346/SRR7050346.sra Written 12935491 spots for SRR7050346/SRR7050346.sra 2020-06-29T14:30:10 prefetch.2.10.7: 1) Downloading 'SRR7050347'... 2020-06-29T14:30:10 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:31:53 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:31:53 prefetch.2.10.7: 'SRR7050347' is valid 2020-06-29T14:31:53 prefetch.2.10.7: 1) 'SRR7050347' was downloaded successfully 2020-06-29T14:31:53 prefetch.2.10.7: 'SRR7050347' has 0 unresolved dependencies Read 12921334 spots for SRR7050347/SRR7050347.sra Written 12921334 spots for SRR7050347/SRR7050347.sra 2020-06-29T14:32:46 prefetch.2.10.7: 1) Downloading 'SRR7050348'... 2020-06-29T14:32:46 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:34:49 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:34:50 prefetch.2.10.7: 'SRR7050348' is valid 2020-06-29T14:34:50 prefetch.2.10.7: 1) 'SRR7050348' was downloaded successfully 2020-06-29T14:34:50 prefetch.2.10.7: 'SRR7050348' has 0 unresolved dependencies Read 13008973 spots for SRR7050348/SRR7050348.sra Written 13008973 spots for SRR7050348/SRR7050348.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:31 38865798 reads; of these: 38865798 (100.00%) were unpaired; of these: 1316995 (3.39%) aligned 0 times 27535867 (70.85%) aligned exactly 1 time 10012936 (25.76%) aligned >1 times 96.61% overall alignment rate Time searching: 00:11:31 Overall time: 00:11:31 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 4049508 / 37548803 = 0.1078 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:03:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981654/SRX3981654.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981654/SRX3981654.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981654/SRX3981654.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981654/SRX3981654.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:03:00: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:03:00: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:03:05: 1000000 INFO @ Tue, 30 Jun 2020 00:03:11: 2000000 INFO @ Tue, 30 Jun 2020 00:03:16: 3000000 INFO @ Tue, 30 Jun 2020 00:03:22: 4000000 INFO @ Tue, 30 Jun 2020 00:03:27: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:03:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981654/SRX3981654.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981654/SRX3981654.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981654/SRX3981654.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981654/SRX3981654.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:03:30: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:03:30: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:03:34: 6000000 INFO @ Tue, 30 Jun 2020 00:03:36: 1000000 INFO @ Tue, 30 Jun 2020 00:03:40: 7000000 INFO @ Tue, 30 Jun 2020 00:03:42: 2000000 INFO @ Tue, 30 Jun 2020 00:03:46: 8000000 INFO @ Tue, 30 Jun 2020 00:03:49: 3000000 INFO @ Tue, 30 Jun 2020 00:03:52: 9000000 INFO @ Tue, 30 Jun 2020 00:03:55: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:03:59: 10000000 INFO @ Tue, 30 Jun 2020 00:04:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981654/SRX3981654.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981654/SRX3981654.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981654/SRX3981654.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981654/SRX3981654.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:04:00: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:04:00: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:04:01: 5000000 INFO @ Tue, 30 Jun 2020 00:04:05: 11000000 INFO @ Tue, 30 Jun 2020 00:04:06: 1000000 INFO @ Tue, 30 Jun 2020 00:04:07: 6000000 INFO @ Tue, 30 Jun 2020 00:04:11: 12000000 INFO @ Tue, 30 Jun 2020 00:04:12: 2000000 INFO @ Tue, 30 Jun 2020 00:04:14: 7000000 INFO @ Tue, 30 Jun 2020 00:04:17: 13000000 INFO @ Tue, 30 Jun 2020 00:04:19: 3000000 INFO @ Tue, 30 Jun 2020 00:04:20: 8000000 INFO @ Tue, 30 Jun 2020 00:04:24: 14000000 INFO @ Tue, 30 Jun 2020 00:04:25: 4000000 INFO @ Tue, 30 Jun 2020 00:04:26: 9000000 INFO @ Tue, 30 Jun 2020 00:04:30: 15000000 INFO @ Tue, 30 Jun 2020 00:04:31: 5000000 INFO @ Tue, 30 Jun 2020 00:04:33: 10000000 INFO @ Tue, 30 Jun 2020 00:04:36: 16000000 INFO @ Tue, 30 Jun 2020 00:04:38: 6000000 INFO @ Tue, 30 Jun 2020 00:04:39: 11000000 INFO @ Tue, 30 Jun 2020 00:04:43: 17000000 INFO @ Tue, 30 Jun 2020 00:04:44: 7000000 INFO @ Tue, 30 Jun 2020 00:04:45: 12000000 INFO @ Tue, 30 Jun 2020 00:04:49: 18000000 INFO @ Tue, 30 Jun 2020 00:04:50: 8000000 INFO @ Tue, 30 Jun 2020 00:04:52: 13000000 INFO @ Tue, 30 Jun 2020 00:04:55: 19000000 INFO @ Tue, 30 Jun 2020 00:04:57: 9000000 INFO @ Tue, 30 Jun 2020 00:04:58: 14000000 INFO @ Tue, 30 Jun 2020 00:05:01: 20000000 INFO @ Tue, 30 Jun 2020 00:05:03: 10000000 INFO @ Tue, 30 Jun 2020 00:05:04: 15000000 INFO @ Tue, 30 Jun 2020 00:05:08: 21000000 INFO @ Tue, 30 Jun 2020 00:05:09: 11000000 INFO @ Tue, 30 Jun 2020 00:05:11: 16000000 INFO @ Tue, 30 Jun 2020 00:05:14: 22000000 INFO @ Tue, 30 Jun 2020 00:05:15: 12000000 INFO @ Tue, 30 Jun 2020 00:05:17: 17000000 INFO @ Tue, 30 Jun 2020 00:05:20: 23000000 INFO @ Tue, 30 Jun 2020 00:05:22: 13000000 INFO @ Tue, 30 Jun 2020 00:05:23: 18000000 INFO @ Tue, 30 Jun 2020 00:05:26: 24000000 INFO @ Tue, 30 Jun 2020 00:05:28: 14000000 INFO @ Tue, 30 Jun 2020 00:05:29: 19000000 INFO @ Tue, 30 Jun 2020 00:05:33: 25000000 INFO @ Tue, 30 Jun 2020 00:05:34: 15000000 INFO @ Tue, 30 Jun 2020 00:05:36: 20000000 INFO @ Tue, 30 Jun 2020 00:05:39: 26000000 INFO @ Tue, 30 Jun 2020 00:05:40: 16000000 INFO @ Tue, 30 Jun 2020 00:05:42: 21000000 INFO @ Tue, 30 Jun 2020 00:05:45: 27000000 INFO @ Tue, 30 Jun 2020 00:05:47: 17000000 INFO @ Tue, 30 Jun 2020 00:05:48: 22000000 INFO @ Tue, 30 Jun 2020 00:05:52: 28000000 INFO @ Tue, 30 Jun 2020 00:05:53: 18000000 INFO @ Tue, 30 Jun 2020 00:05:54: 23000000 INFO @ Tue, 30 Jun 2020 00:05:58: 29000000 INFO @ Tue, 30 Jun 2020 00:05:59: 19000000 INFO @ Tue, 30 Jun 2020 00:06:01: 24000000 INFO @ Tue, 30 Jun 2020 00:06:04: 30000000 INFO @ Tue, 30 Jun 2020 00:06:06: 20000000 INFO @ Tue, 30 Jun 2020 00:06:07: 25000000 INFO @ Tue, 30 Jun 2020 00:06:11: 31000000 INFO @ Tue, 30 Jun 2020 00:06:12: 21000000 INFO @ Tue, 30 Jun 2020 00:06:13: 26000000 INFO @ Tue, 30 Jun 2020 00:06:17: 32000000 INFO @ Tue, 30 Jun 2020 00:06:18: 22000000 INFO @ Tue, 30 Jun 2020 00:06:20: 27000000 INFO @ Tue, 30 Jun 2020 00:06:23: 33000000 INFO @ Tue, 30 Jun 2020 00:06:24: 23000000 INFO @ Tue, 30 Jun 2020 00:06:26: 28000000 INFO @ Tue, 30 Jun 2020 00:06:27: #1 tag size is determined as 51 bps INFO @ Tue, 30 Jun 2020 00:06:27: #1 tag size = 51 INFO @ Tue, 30 Jun 2020 00:06:27: #1 total tags in treatment: 33499295 INFO @ Tue, 30 Jun 2020 00:06:27: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:06:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:06:27: #1 tags after filtering in treatment: 33499295 INFO @ Tue, 30 Jun 2020 00:06:27: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:06:27: #1 finished! INFO @ Tue, 30 Jun 2020 00:06:27: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:06:27: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:06:29: #2 number of paired peaks: 17 WARNING @ Tue, 30 Jun 2020 00:06:29: Too few paired peaks (17) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:06:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981654/SRX3981654.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981654/SRX3981654.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981654/SRX3981654.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981654/SRX3981654.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:06:31: 24000000 INFO @ Tue, 30 Jun 2020 00:06:33: 29000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 00:06:38: 25000000 INFO @ Tue, 30 Jun 2020 00:06:40: 30000000 INFO @ Tue, 30 Jun 2020 00:06:44: 26000000 INFO @ Tue, 30 Jun 2020 00:06:46: 31000000 INFO @ Tue, 30 Jun 2020 00:06:51: 27000000 INFO @ Tue, 30 Jun 2020 00:06:53: 32000000 INFO @ Tue, 30 Jun 2020 00:06:57: 28000000 INFO @ Tue, 30 Jun 2020 00:06:59: 33000000 INFO @ Tue, 30 Jun 2020 00:07:03: #1 tag size is determined as 51 bps INFO @ Tue, 30 Jun 2020 00:07:03: #1 tag size = 51 INFO @ Tue, 30 Jun 2020 00:07:03: #1 total tags in treatment: 33499295 INFO @ Tue, 30 Jun 2020 00:07:03: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:07:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:07:03: #1 tags after filtering in treatment: 33499295 INFO @ Tue, 30 Jun 2020 00:07:03: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:07:03: #1 finished! INFO @ Tue, 30 Jun 2020 00:07:03: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:07:03: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:07:04: 29000000 INFO @ Tue, 30 Jun 2020 00:07:05: #2 number of paired peaks: 17 WARNING @ Tue, 30 Jun 2020 00:07:05: Too few paired peaks (17) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:07:05: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981654/SRX3981654.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981654/SRX3981654.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981654/SRX3981654.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981654/SRX3981654.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:07:10: 30000000 INFO @ Tue, 30 Jun 2020 00:07:17: 31000000 INFO @ Tue, 30 Jun 2020 00:07:23: 32000000 INFO @ Tue, 30 Jun 2020 00:07:29: 33000000 INFO @ Tue, 30 Jun 2020 00:07:32: #1 tag size is determined as 51 bps INFO @ Tue, 30 Jun 2020 00:07:32: #1 tag size = 51 INFO @ Tue, 30 Jun 2020 00:07:32: #1 total tags in treatment: 33499295 INFO @ Tue, 30 Jun 2020 00:07:32: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:07:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:07:32: #1 tags after filtering in treatment: 33499295 INFO @ Tue, 30 Jun 2020 00:07:32: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:07:32: #1 finished! INFO @ Tue, 30 Jun 2020 00:07:32: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:07:32: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:07:34: #2 number of paired peaks: 17 WARNING @ Tue, 30 Jun 2020 00:07:34: Too few paired peaks (17) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:07:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981654/SRX3981654.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981654/SRX3981654.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981654/SRX3981654.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981654/SRX3981654.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。