Job ID = 6528033 SRX = SRX3981650 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:10:50 prefetch.2.10.7: 1) Downloading 'SRR7050334'... 2020-06-29T14:10:50 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:11:52 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:11:52 prefetch.2.10.7: 'SRR7050334' is valid 2020-06-29T14:11:52 prefetch.2.10.7: 1) 'SRR7050334' was downloaded successfully 2020-06-29T14:11:52 prefetch.2.10.7: 'SRR7050334' has 0 unresolved dependencies Read 12135503 spots for SRR7050334/SRR7050334.sra Written 12135503 spots for SRR7050334/SRR7050334.sra 2020-06-29T14:12:42 prefetch.2.10.7: 1) Downloading 'SRR7050335'... 2020-06-29T14:12:42 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:13:37 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:13:37 prefetch.2.10.7: 'SRR7050335' is valid 2020-06-29T14:13:37 prefetch.2.10.7: 1) 'SRR7050335' was downloaded successfully 2020-06-29T14:13:37 prefetch.2.10.7: 'SRR7050335' has 0 unresolved dependencies Read 12122228 spots for SRR7050335/SRR7050335.sra Written 12122228 spots for SRR7050335/SRR7050335.sra 2020-06-29T14:14:25 prefetch.2.10.7: 1) Downloading 'SRR7050336'... 2020-06-29T14:14:25 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:15:23 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:15:24 prefetch.2.10.7: 'SRR7050336' is valid 2020-06-29T14:15:24 prefetch.2.10.7: 1) 'SRR7050336' was downloaded successfully 2020-06-29T14:15:24 prefetch.2.10.7: 'SRR7050336' has 0 unresolved dependencies Read 12220200 spots for SRR7050336/SRR7050336.sra Written 12220200 spots for SRR7050336/SRR7050336.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:18 36477931 reads; of these: 36477931 (100.00%) were unpaired; of these: 973868 (2.67%) aligned 0 times 25981705 (71.23%) aligned exactly 1 time 9522358 (26.10%) aligned >1 times 97.33% overall alignment rate Time searching: 00:11:18 Overall time: 00:11:18 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 4362604 / 35504063 = 0.1229 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:42:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981650/SRX3981650.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981650/SRX3981650.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981650/SRX3981650.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981650/SRX3981650.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:42:35: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:42:35: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:42:40: 1000000 INFO @ Mon, 29 Jun 2020 23:42:45: 2000000 INFO @ Mon, 29 Jun 2020 23:42:51: 3000000 INFO @ Mon, 29 Jun 2020 23:42:56: 4000000 INFO @ Mon, 29 Jun 2020 23:43:01: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:43:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981650/SRX3981650.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981650/SRX3981650.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981650/SRX3981650.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981650/SRX3981650.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:43:05: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:43:05: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:43:06: 6000000 INFO @ Mon, 29 Jun 2020 23:43:10: 1000000 INFO @ Mon, 29 Jun 2020 23:43:11: 7000000 INFO @ Mon, 29 Jun 2020 23:43:16: 2000000 INFO @ Mon, 29 Jun 2020 23:43:17: 8000000 INFO @ Mon, 29 Jun 2020 23:43:21: 3000000 INFO @ Mon, 29 Jun 2020 23:43:22: 9000000 INFO @ Mon, 29 Jun 2020 23:43:27: 4000000 INFO @ Mon, 29 Jun 2020 23:43:27: 10000000 INFO @ Mon, 29 Jun 2020 23:43:32: 5000000 INFO @ Mon, 29 Jun 2020 23:43:33: 11000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:43:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981650/SRX3981650.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981650/SRX3981650.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981650/SRX3981650.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981650/SRX3981650.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:43:35: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:43:35: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:43:37: 6000000 INFO @ Mon, 29 Jun 2020 23:43:38: 12000000 INFO @ Mon, 29 Jun 2020 23:43:40: 1000000 INFO @ Mon, 29 Jun 2020 23:43:43: 7000000 INFO @ Mon, 29 Jun 2020 23:43:43: 13000000 INFO @ Mon, 29 Jun 2020 23:43:46: 2000000 INFO @ Mon, 29 Jun 2020 23:43:48: 8000000 INFO @ Mon, 29 Jun 2020 23:43:49: 14000000 INFO @ Mon, 29 Jun 2020 23:43:51: 3000000 INFO @ Mon, 29 Jun 2020 23:43:54: 9000000 INFO @ Mon, 29 Jun 2020 23:43:54: 15000000 INFO @ Mon, 29 Jun 2020 23:43:56: 4000000 INFO @ Mon, 29 Jun 2020 23:43:59: 10000000 INFO @ Mon, 29 Jun 2020 23:44:00: 16000000 INFO @ Mon, 29 Jun 2020 23:44:02: 5000000 INFO @ Mon, 29 Jun 2020 23:44:04: 11000000 INFO @ Mon, 29 Jun 2020 23:44:05: 17000000 INFO @ Mon, 29 Jun 2020 23:44:07: 6000000 INFO @ Mon, 29 Jun 2020 23:44:10: 12000000 INFO @ Mon, 29 Jun 2020 23:44:10: 18000000 INFO @ Mon, 29 Jun 2020 23:44:13: 7000000 INFO @ Mon, 29 Jun 2020 23:44:15: 13000000 INFO @ Mon, 29 Jun 2020 23:44:16: 19000000 INFO @ Mon, 29 Jun 2020 23:44:18: 8000000 INFO @ Mon, 29 Jun 2020 23:44:21: 14000000 INFO @ Mon, 29 Jun 2020 23:44:21: 20000000 INFO @ Mon, 29 Jun 2020 23:44:23: 9000000 INFO @ Mon, 29 Jun 2020 23:44:26: 15000000 INFO @ Mon, 29 Jun 2020 23:44:27: 21000000 INFO @ Mon, 29 Jun 2020 23:44:29: 10000000 INFO @ Mon, 29 Jun 2020 23:44:31: 16000000 INFO @ Mon, 29 Jun 2020 23:44:32: 22000000 INFO @ Mon, 29 Jun 2020 23:44:34: 11000000 INFO @ Mon, 29 Jun 2020 23:44:37: 17000000 INFO @ Mon, 29 Jun 2020 23:44:37: 23000000 INFO @ Mon, 29 Jun 2020 23:44:40: 12000000 INFO @ Mon, 29 Jun 2020 23:44:42: 18000000 INFO @ Mon, 29 Jun 2020 23:44:43: 24000000 INFO @ Mon, 29 Jun 2020 23:44:45: 13000000 INFO @ Mon, 29 Jun 2020 23:44:48: 19000000 INFO @ Mon, 29 Jun 2020 23:44:48: 25000000 INFO @ Mon, 29 Jun 2020 23:44:51: 14000000 INFO @ Mon, 29 Jun 2020 23:44:53: 20000000 INFO @ Mon, 29 Jun 2020 23:44:54: 26000000 INFO @ Mon, 29 Jun 2020 23:44:56: 15000000 INFO @ Mon, 29 Jun 2020 23:44:59: 21000000 INFO @ Mon, 29 Jun 2020 23:44:59: 27000000 INFO @ Mon, 29 Jun 2020 23:45:02: 16000000 INFO @ Mon, 29 Jun 2020 23:45:04: 22000000 INFO @ Mon, 29 Jun 2020 23:45:05: 28000000 INFO @ Mon, 29 Jun 2020 23:45:07: 17000000 INFO @ Mon, 29 Jun 2020 23:45:10: 23000000 INFO @ Mon, 29 Jun 2020 23:45:10: 29000000 INFO @ Mon, 29 Jun 2020 23:45:13: 18000000 INFO @ Mon, 29 Jun 2020 23:45:15: 30000000 INFO @ Mon, 29 Jun 2020 23:45:16: 24000000 INFO @ Mon, 29 Jun 2020 23:45:18: 19000000 INFO @ Mon, 29 Jun 2020 23:45:21: 31000000 INFO @ Mon, 29 Jun 2020 23:45:21: 25000000 INFO @ Mon, 29 Jun 2020 23:45:22: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:45:22: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:45:22: #1 total tags in treatment: 31141459 INFO @ Mon, 29 Jun 2020 23:45:22: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:45:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:45:22: #1 tags after filtering in treatment: 31141459 INFO @ Mon, 29 Jun 2020 23:45:22: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:45:22: #1 finished! INFO @ Mon, 29 Jun 2020 23:45:22: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:45:22: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:45:24: 20000000 INFO @ Mon, 29 Jun 2020 23:45:24: #2 number of paired peaks: 37 WARNING @ Mon, 29 Jun 2020 23:45:24: Too few paired peaks (37) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:45:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981650/SRX3981650.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981650/SRX3981650.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981650/SRX3981650.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981650/SRX3981650.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:45:27: 26000000 INFO @ Mon, 29 Jun 2020 23:45:29: 21000000 INFO @ Mon, 29 Jun 2020 23:45:32: 27000000 INFO @ Mon, 29 Jun 2020 23:45:35: 22000000 INFO @ Mon, 29 Jun 2020 23:45:37: 28000000 INFO @ Mon, 29 Jun 2020 23:45:40: 23000000 INFO @ Mon, 29 Jun 2020 23:45:43: 29000000 INFO @ Mon, 29 Jun 2020 23:45:45: 24000000 INFO @ Mon, 29 Jun 2020 23:45:48: 30000000 INFO @ Mon, 29 Jun 2020 23:45:51: 25000000 INFO @ Mon, 29 Jun 2020 23:45:54: 31000000 INFO @ Mon, 29 Jun 2020 23:45:55: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:45:55: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:45:55: #1 total tags in treatment: 31141459 INFO @ Mon, 29 Jun 2020 23:45:55: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:45:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:45:55: #1 tags after filtering in treatment: 31141459 INFO @ Mon, 29 Jun 2020 23:45:55: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:45:55: #1 finished! INFO @ Mon, 29 Jun 2020 23:45:55: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:45:55: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:45:56: 26000000 INFO @ Mon, 29 Jun 2020 23:45:57: #2 number of paired peaks: 37 WARNING @ Mon, 29 Jun 2020 23:45:57: Too few paired peaks (37) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:45:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981650/SRX3981650.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981650/SRX3981650.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981650/SRX3981650.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981650/SRX3981650.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:46:02: 27000000 INFO @ Mon, 29 Jun 2020 23:46:07: 28000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 23:46:13: 29000000 INFO @ Mon, 29 Jun 2020 23:46:18: 30000000 INFO @ Mon, 29 Jun 2020 23:46:23: 31000000 INFO @ Mon, 29 Jun 2020 23:46:24: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:46:24: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:46:24: #1 total tags in treatment: 31141459 INFO @ Mon, 29 Jun 2020 23:46:24: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:46:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:46:25: #1 tags after filtering in treatment: 31141459 INFO @ Mon, 29 Jun 2020 23:46:25: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:46:25: #1 finished! INFO @ Mon, 29 Jun 2020 23:46:25: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:46:25: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:46:27: #2 number of paired peaks: 37 WARNING @ Mon, 29 Jun 2020 23:46:27: Too few paired peaks (37) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:46:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981650/SRX3981650.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981650/SRX3981650.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981650/SRX3981650.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981650/SRX3981650.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。