Job ID = 6528030 SRX = SRX3981638 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:09:35 prefetch.2.10.7: 1) Downloading 'SRR7050298'... 2020-06-29T14:09:35 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:11:04 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:11:05 prefetch.2.10.7: 'SRR7050298' is valid 2020-06-29T14:11:05 prefetch.2.10.7: 1) 'SRR7050298' was downloaded successfully 2020-06-29T14:11:05 prefetch.2.10.7: 'SRR7050298' has 0 unresolved dependencies Read 13591701 spots for SRR7050298/SRR7050298.sra Written 13591701 spots for SRR7050298/SRR7050298.sra 2020-06-29T14:12:00 prefetch.2.10.7: 1) Downloading 'SRR7050299'... 2020-06-29T14:12:00 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:13:02 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:13:02 prefetch.2.10.7: 'SRR7050299' is valid 2020-06-29T14:13:02 prefetch.2.10.7: 1) 'SRR7050299' was downloaded successfully 2020-06-29T14:13:02 prefetch.2.10.7: 'SRR7050299' has 0 unresolved dependencies Read 13469267 spots for SRR7050299/SRR7050299.sra Written 13469267 spots for SRR7050299/SRR7050299.sra 2020-06-29T14:13:57 prefetch.2.10.7: 1) Downloading 'SRR7050300'... 2020-06-29T14:13:57 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:14:58 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:14:59 prefetch.2.10.7: 'SRR7050300' is valid 2020-06-29T14:14:59 prefetch.2.10.7: 1) 'SRR7050300' was downloaded successfully 2020-06-29T14:14:59 prefetch.2.10.7: 'SRR7050300' has 0 unresolved dependencies Read 13459860 spots for SRR7050300/SRR7050300.sra Written 13459860 spots for SRR7050300/SRR7050300.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:12:51 40520828 reads; of these: 40520828 (100.00%) were unpaired; of these: 1130062 (2.79%) aligned 0 times 30496265 (75.26%) aligned exactly 1 time 8894501 (21.95%) aligned >1 times 97.21% overall alignment rate Time searching: 00:12:51 Overall time: 00:12:51 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 4622445 / 39390766 = 0.1173 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:46:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981638/SRX3981638.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981638/SRX3981638.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981638/SRX3981638.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981638/SRX3981638.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:46:04: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:46:04: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:46:12: 1000000 INFO @ Mon, 29 Jun 2020 23:46:19: 2000000 INFO @ Mon, 29 Jun 2020 23:46:26: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:46:34: 4000000 INFO @ Mon, 29 Jun 2020 23:46:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981638/SRX3981638.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981638/SRX3981638.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981638/SRX3981638.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981638/SRX3981638.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:46:34: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:46:34: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:46:41: 1000000 INFO @ Mon, 29 Jun 2020 23:46:42: 5000000 INFO @ Mon, 29 Jun 2020 23:46:48: 2000000 INFO @ Mon, 29 Jun 2020 23:46:50: 6000000 INFO @ Mon, 29 Jun 2020 23:46:55: 3000000 INFO @ Mon, 29 Jun 2020 23:46:58: 7000000 INFO @ Mon, 29 Jun 2020 23:47:01: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:47:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981638/SRX3981638.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981638/SRX3981638.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981638/SRX3981638.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981638/SRX3981638.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:47:04: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:47:04: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:47:06: 8000000 INFO @ Mon, 29 Jun 2020 23:47:08: 5000000 INFO @ Mon, 29 Jun 2020 23:47:13: 1000000 INFO @ Mon, 29 Jun 2020 23:47:14: 9000000 INFO @ Mon, 29 Jun 2020 23:47:15: 6000000 INFO @ Mon, 29 Jun 2020 23:47:21: 7000000 INFO @ Mon, 29 Jun 2020 23:47:22: 2000000 INFO @ Mon, 29 Jun 2020 23:47:23: 10000000 INFO @ Mon, 29 Jun 2020 23:47:28: 8000000 INFO @ Mon, 29 Jun 2020 23:47:30: 3000000 INFO @ Mon, 29 Jun 2020 23:47:31: 11000000 INFO @ Mon, 29 Jun 2020 23:47:35: 9000000 INFO @ Mon, 29 Jun 2020 23:47:37: 4000000 INFO @ Mon, 29 Jun 2020 23:47:39: 12000000 INFO @ Mon, 29 Jun 2020 23:47:42: 10000000 INFO @ Mon, 29 Jun 2020 23:47:45: 5000000 INFO @ Mon, 29 Jun 2020 23:47:47: 13000000 INFO @ Mon, 29 Jun 2020 23:47:49: 11000000 INFO @ Mon, 29 Jun 2020 23:47:52: 6000000 INFO @ Mon, 29 Jun 2020 23:47:55: 14000000 INFO @ Mon, 29 Jun 2020 23:47:55: 12000000 INFO @ Mon, 29 Jun 2020 23:48:00: 7000000 INFO @ Mon, 29 Jun 2020 23:48:02: 13000000 INFO @ Mon, 29 Jun 2020 23:48:03: 15000000 INFO @ Mon, 29 Jun 2020 23:48:07: 8000000 INFO @ Mon, 29 Jun 2020 23:48:09: 14000000 INFO @ Mon, 29 Jun 2020 23:48:11: 16000000 INFO @ Mon, 29 Jun 2020 23:48:15: 9000000 INFO @ Mon, 29 Jun 2020 23:48:16: 15000000 INFO @ Mon, 29 Jun 2020 23:48:19: 17000000 INFO @ Mon, 29 Jun 2020 23:48:22: 10000000 INFO @ Mon, 29 Jun 2020 23:48:23: 16000000 INFO @ Mon, 29 Jun 2020 23:48:28: 18000000 INFO @ Mon, 29 Jun 2020 23:48:30: 11000000 INFO @ Mon, 29 Jun 2020 23:48:30: 17000000 INFO @ Mon, 29 Jun 2020 23:48:36: 19000000 INFO @ Mon, 29 Jun 2020 23:48:37: 18000000 INFO @ Mon, 29 Jun 2020 23:48:37: 12000000 INFO @ Mon, 29 Jun 2020 23:48:44: 19000000 INFO @ Mon, 29 Jun 2020 23:48:44: 20000000 INFO @ Mon, 29 Jun 2020 23:48:45: 13000000 INFO @ Mon, 29 Jun 2020 23:48:50: 20000000 INFO @ Mon, 29 Jun 2020 23:48:52: 21000000 INFO @ Mon, 29 Jun 2020 23:48:52: 14000000 INFO @ Mon, 29 Jun 2020 23:48:57: 21000000 INFO @ Mon, 29 Jun 2020 23:49:00: 22000000 INFO @ Mon, 29 Jun 2020 23:49:00: 15000000 INFO @ Mon, 29 Jun 2020 23:49:04: 22000000 INFO @ Mon, 29 Jun 2020 23:49:07: 23000000 INFO @ Mon, 29 Jun 2020 23:49:08: 16000000 INFO @ Mon, 29 Jun 2020 23:49:11: 23000000 INFO @ Mon, 29 Jun 2020 23:49:15: 24000000 INFO @ Mon, 29 Jun 2020 23:49:15: 17000000 INFO @ Mon, 29 Jun 2020 23:49:18: 24000000 INFO @ Mon, 29 Jun 2020 23:49:23: 18000000 INFO @ Mon, 29 Jun 2020 23:49:23: 25000000 INFO @ Mon, 29 Jun 2020 23:49:24: 25000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 23:49:31: 19000000 INFO @ Mon, 29 Jun 2020 23:49:31: 26000000 INFO @ Mon, 29 Jun 2020 23:49:32: 26000000 INFO @ Mon, 29 Jun 2020 23:49:38: 20000000 INFO @ Mon, 29 Jun 2020 23:49:39: 27000000 INFO @ Mon, 29 Jun 2020 23:49:39: 27000000 INFO @ Mon, 29 Jun 2020 23:49:46: 21000000 INFO @ Mon, 29 Jun 2020 23:49:48: 28000000 INFO @ Mon, 29 Jun 2020 23:49:48: 28000000 INFO @ Mon, 29 Jun 2020 23:49:53: 22000000 INFO @ Mon, 29 Jun 2020 23:49:55: 29000000 INFO @ Mon, 29 Jun 2020 23:49:56: 29000000 INFO @ Mon, 29 Jun 2020 23:50:01: 23000000 INFO @ Mon, 29 Jun 2020 23:50:03: 30000000 INFO @ Mon, 29 Jun 2020 23:50:04: 30000000 INFO @ Mon, 29 Jun 2020 23:50:08: 24000000 INFO @ Mon, 29 Jun 2020 23:50:10: 31000000 INFO @ Mon, 29 Jun 2020 23:50:11: 31000000 INFO @ Mon, 29 Jun 2020 23:50:16: 25000000 INFO @ Mon, 29 Jun 2020 23:50:16: 32000000 INFO @ Mon, 29 Jun 2020 23:50:19: 32000000 INFO @ Mon, 29 Jun 2020 23:50:23: 26000000 INFO @ Mon, 29 Jun 2020 23:50:23: 33000000 INFO @ Mon, 29 Jun 2020 23:50:26: 33000000 INFO @ Mon, 29 Jun 2020 23:50:30: 34000000 INFO @ Mon, 29 Jun 2020 23:50:30: 27000000 INFO @ Mon, 29 Jun 2020 23:50:33: 34000000 INFO @ Mon, 29 Jun 2020 23:50:35: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:50:35: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:50:35: #1 total tags in treatment: 34768321 INFO @ Mon, 29 Jun 2020 23:50:35: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:50:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:50:36: #1 tags after filtering in treatment: 34768321 INFO @ Mon, 29 Jun 2020 23:50:36: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:50:36: #1 finished! INFO @ Mon, 29 Jun 2020 23:50:36: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:50:36: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:50:38: #2 number of paired peaks: 63 WARNING @ Mon, 29 Jun 2020 23:50:38: Too few paired peaks (63) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:50:38: Process for pairing-model is terminated! INFO @ Mon, 29 Jun 2020 23:50:38: 28000000 cut: /home/okishinya/chipatlas/results/dm3/SRX3981638/SRX3981638.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981638/SRX3981638.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981638/SRX3981638.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981638/SRX3981638.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:50:39: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:50:39: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:50:39: #1 total tags in treatment: 34768321 INFO @ Mon, 29 Jun 2020 23:50:39: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:50:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:50:40: #1 tags after filtering in treatment: 34768321 INFO @ Mon, 29 Jun 2020 23:50:40: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:50:40: #1 finished! INFO @ Mon, 29 Jun 2020 23:50:40: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:50:40: #2 looking for paired plus/minus strand peaks... BigWig に変換しました。 INFO @ Mon, 29 Jun 2020 23:50:42: #2 number of paired peaks: 63 WARNING @ Mon, 29 Jun 2020 23:50:42: Too few paired peaks (63) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:50:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981638/SRX3981638.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981638/SRX3981638.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981638/SRX3981638.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981638/SRX3981638.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:50:45: 29000000 INFO @ Mon, 29 Jun 2020 23:50:52: 30000000 INFO @ Mon, 29 Jun 2020 23:50:59: 31000000 INFO @ Mon, 29 Jun 2020 23:51:06: 32000000 INFO @ Mon, 29 Jun 2020 23:51:13: 33000000 INFO @ Mon, 29 Jun 2020 23:51:19: 34000000 INFO @ Mon, 29 Jun 2020 23:51:25: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:51:25: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:51:25: #1 total tags in treatment: 34768321 INFO @ Mon, 29 Jun 2020 23:51:25: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:51:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:51:25: #1 tags after filtering in treatment: 34768321 INFO @ Mon, 29 Jun 2020 23:51:25: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:51:25: #1 finished! INFO @ Mon, 29 Jun 2020 23:51:25: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:51:25: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:51:27: #2 number of paired peaks: 63 WARNING @ Mon, 29 Jun 2020 23:51:27: Too few paired peaks (63) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:51:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981638/SRX3981638.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981638/SRX3981638.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981638/SRX3981638.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981638/SRX3981638.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling