Job ID = 2590365 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 14,469,112 reads read : 28,938,224 reads written : 14,469,112 reads 0-length : 14,469,112 spots read : 14,364,727 reads read : 28,729,454 reads written : 14,364,727 reads 0-length : 14,364,727 spots read : 14,328,526 reads read : 28,657,052 reads written : 14,328,526 reads 0-length : 14,328,526 fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:15:08 43162365 reads; of these: 43162365 (100.00%) were unpaired; of these: 1258983 (2.92%) aligned 0 times 31865149 (73.83%) aligned exactly 1 time 10038233 (23.26%) aligned >1 times 97.08% overall alignment rate Time searching: 00:15:09 Overall time: 00:15:09 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 4816049 / 41903382 = 0.1149 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 12 Aug 2019 21:40:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981637/SRX3981637.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981637/SRX3981637.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981637/SRX3981637.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981637/SRX3981637.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 21:40:36: #1 read tag files... INFO @ Mon, 12 Aug 2019 21:40:36: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 21:40:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981637/SRX3981637.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981637/SRX3981637.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981637/SRX3981637.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981637/SRX3981637.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 21:40:37: #1 read tag files... INFO @ Mon, 12 Aug 2019 21:40:37: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 21:40:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981637/SRX3981637.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981637/SRX3981637.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981637/SRX3981637.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981637/SRX3981637.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 21:40:38: #1 read tag files... INFO @ Mon, 12 Aug 2019 21:40:38: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 21:40:45: 1000000 INFO @ Mon, 12 Aug 2019 21:40:45: 1000000 INFO @ Mon, 12 Aug 2019 21:40:45: 1000000 INFO @ Mon, 12 Aug 2019 21:40:52: 2000000 INFO @ Mon, 12 Aug 2019 21:40:53: 2000000 INFO @ Mon, 12 Aug 2019 21:40:53: 2000000 INFO @ Mon, 12 Aug 2019 21:40:58: 3000000 INFO @ Mon, 12 Aug 2019 21:41:01: 3000000 INFO @ Mon, 12 Aug 2019 21:41:01: 3000000 INFO @ Mon, 12 Aug 2019 21:41:05: 4000000 INFO @ Mon, 12 Aug 2019 21:41:09: 4000000 INFO @ Mon, 12 Aug 2019 21:41:10: 4000000 INFO @ Mon, 12 Aug 2019 21:41:11: 5000000 INFO @ Mon, 12 Aug 2019 21:41:18: 5000000 INFO @ Mon, 12 Aug 2019 21:41:18: 6000000 INFO @ Mon, 12 Aug 2019 21:41:18: 5000000 INFO @ Mon, 12 Aug 2019 21:41:24: 7000000 INFO @ Mon, 12 Aug 2019 21:41:26: 6000000 INFO @ Mon, 12 Aug 2019 21:41:26: 6000000 INFO @ Mon, 12 Aug 2019 21:41:31: 8000000 INFO @ Mon, 12 Aug 2019 21:41:34: 7000000 INFO @ Mon, 12 Aug 2019 21:41:34: 7000000 INFO @ Mon, 12 Aug 2019 21:41:38: 9000000 INFO @ Mon, 12 Aug 2019 21:41:41: 8000000 INFO @ Mon, 12 Aug 2019 21:41:43: 8000000 INFO @ Mon, 12 Aug 2019 21:41:44: 10000000 INFO @ Mon, 12 Aug 2019 21:41:49: 9000000 INFO @ Mon, 12 Aug 2019 21:41:51: 9000000 INFO @ Mon, 12 Aug 2019 21:41:51: 11000000 INFO @ Mon, 12 Aug 2019 21:41:57: 10000000 INFO @ Mon, 12 Aug 2019 21:41:58: 12000000 INFO @ Mon, 12 Aug 2019 21:41:59: 10000000 INFO @ Mon, 12 Aug 2019 21:42:04: 13000000 INFO @ Mon, 12 Aug 2019 21:42:04: 11000000 INFO @ Mon, 12 Aug 2019 21:42:07: 11000000 INFO @ Mon, 12 Aug 2019 21:42:11: 14000000 INFO @ Mon, 12 Aug 2019 21:42:12: 12000000 INFO @ Mon, 12 Aug 2019 21:42:14: 12000000 INFO @ Mon, 12 Aug 2019 21:42:18: 15000000 INFO @ Mon, 12 Aug 2019 21:42:20: 13000000 INFO @ Mon, 12 Aug 2019 21:42:23: 13000000 INFO @ Mon, 12 Aug 2019 21:42:24: 16000000 INFO @ Mon, 12 Aug 2019 21:42:29: 14000000 INFO @ Mon, 12 Aug 2019 21:42:31: 17000000 INFO @ Mon, 12 Aug 2019 21:42:32: 14000000 INFO @ Mon, 12 Aug 2019 21:42:37: 15000000 INFO @ Mon, 12 Aug 2019 21:42:40: 18000000 INFO @ Mon, 12 Aug 2019 21:42:41: 15000000 INFO @ Mon, 12 Aug 2019 21:42:45: 16000000 INFO @ Mon, 12 Aug 2019 21:42:49: 19000000 INFO @ Mon, 12 Aug 2019 21:42:49: 16000000 INFO @ Mon, 12 Aug 2019 21:42:53: 17000000 INFO @ Mon, 12 Aug 2019 21:42:57: 20000000 INFO @ Mon, 12 Aug 2019 21:42:58: 17000000 INFO @ Mon, 12 Aug 2019 21:43:01: 18000000 INFO @ Mon, 12 Aug 2019 21:43:05: 21000000 INFO @ Mon, 12 Aug 2019 21:43:06: 18000000 INFO @ Mon, 12 Aug 2019 21:43:09: 19000000 INFO @ Mon, 12 Aug 2019 21:43:11: 22000000 INFO @ Mon, 12 Aug 2019 21:43:14: 19000000 INFO @ Mon, 12 Aug 2019 21:43:16: 20000000 INFO @ Mon, 12 Aug 2019 21:43:17: 23000000 INFO @ Mon, 12 Aug 2019 21:43:22: 20000000 INFO @ Mon, 12 Aug 2019 21:43:24: 24000000 INFO @ Mon, 12 Aug 2019 21:43:24: 21000000 INFO @ Mon, 12 Aug 2019 21:43:30: 21000000 INFO @ Mon, 12 Aug 2019 21:43:30: 25000000 INFO @ Mon, 12 Aug 2019 21:43:32: 22000000 INFO @ Mon, 12 Aug 2019 21:43:37: 26000000 INFO @ Mon, 12 Aug 2019 21:43:38: 22000000 INFO @ Mon, 12 Aug 2019 21:43:39: 23000000 INFO @ Mon, 12 Aug 2019 21:43:43: 27000000 INFO @ Mon, 12 Aug 2019 21:43:45: 23000000 INFO @ Mon, 12 Aug 2019 21:43:47: 24000000 INFO @ Mon, 12 Aug 2019 21:43:49: 28000000 INFO @ Mon, 12 Aug 2019 21:43:53: 24000000 INFO @ Mon, 12 Aug 2019 21:43:55: 25000000 INFO @ Mon, 12 Aug 2019 21:43:56: 29000000 INFO @ Mon, 12 Aug 2019 21:44:01: 25000000 INFO @ Mon, 12 Aug 2019 21:44:02: 26000000 INFO @ Mon, 12 Aug 2019 21:44:03: 30000000 INFO @ Mon, 12 Aug 2019 21:44:09: 26000000 INFO @ Mon, 12 Aug 2019 21:44:10: 27000000 INFO @ Mon, 12 Aug 2019 21:44:10: 31000000 INFO @ Mon, 12 Aug 2019 21:44:16: 32000000 INFO @ Mon, 12 Aug 2019 21:44:17: 27000000 INFO @ Mon, 12 Aug 2019 21:44:17: 28000000 INFO @ Mon, 12 Aug 2019 21:44:23: 33000000 INFO @ Mon, 12 Aug 2019 21:44:25: 28000000 INFO @ Mon, 12 Aug 2019 21:44:25: 29000000 INFO @ Mon, 12 Aug 2019 21:44:30: 34000000 INFO @ Mon, 12 Aug 2019 21:44:33: 30000000 INFO @ Mon, 12 Aug 2019 21:44:33: 29000000 INFO @ Mon, 12 Aug 2019 21:44:37: 35000000 INFO @ Mon, 12 Aug 2019 21:44:40: 30000000 INFO @ Mon, 12 Aug 2019 21:44:41: 31000000 INFO @ Mon, 12 Aug 2019 21:44:43: 36000000 INFO @ Mon, 12 Aug 2019 21:44:49: 31000000 INFO @ Mon, 12 Aug 2019 21:44:49: 32000000 INFO @ Mon, 12 Aug 2019 21:44:50: 37000000 INFO @ Mon, 12 Aug 2019 21:44:51: #1 tag size is determined as 51 bps INFO @ Mon, 12 Aug 2019 21:44:51: #1 tag size = 51 INFO @ Mon, 12 Aug 2019 21:44:51: #1 total tags in treatment: 37087333 INFO @ Mon, 12 Aug 2019 21:44:51: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 21:44:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 21:44:52: #1 tags after filtering in treatment: 37087333 INFO @ Mon, 12 Aug 2019 21:44:52: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 21:44:52: #1 finished! INFO @ Mon, 12 Aug 2019 21:44:52: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 21:44:52: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 21:44:55: #2 number of paired peaks: 21 WARNING @ Mon, 12 Aug 2019 21:44:55: Too few paired peaks (21) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 21:44:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981637/SRX3981637.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981637/SRX3981637.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981637/SRX3981637.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981637/SRX3981637.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 21:44:57: 33000000 INFO @ Mon, 12 Aug 2019 21:44:57: 32000000 INFO @ Mon, 12 Aug 2019 21:45:05: 34000000 INFO @ Mon, 12 Aug 2019 21:45:06: 33000000 INFO @ Mon, 12 Aug 2019 21:45:14: 35000000 INFO @ Mon, 12 Aug 2019 21:45:14: 34000000 INFO @ Mon, 12 Aug 2019 21:45:22: 36000000 INFO @ Mon, 12 Aug 2019 21:45:23: 35000000 INFO @ Mon, 12 Aug 2019 21:45:30: 37000000 INFO @ Mon, 12 Aug 2019 21:45:31: #1 tag size is determined as 51 bps INFO @ Mon, 12 Aug 2019 21:45:31: #1 tag size = 51 INFO @ Mon, 12 Aug 2019 21:45:31: #1 total tags in treatment: 37087333 INFO @ Mon, 12 Aug 2019 21:45:31: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 21:45:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 21:45:31: 36000000 INFO @ Mon, 12 Aug 2019 21:45:32: #1 tags after filtering in treatment: 37087333 INFO @ Mon, 12 Aug 2019 21:45:32: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 21:45:32: #1 finished! INFO @ Mon, 12 Aug 2019 21:45:32: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 21:45:32: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 21:45:35: #2 number of paired peaks: 21 WARNING @ Mon, 12 Aug 2019 21:45:35: Too few paired peaks (21) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 21:45:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981637/SRX3981637.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981637/SRX3981637.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981637/SRX3981637.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981637/SRX3981637.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 21:45:40: 37000000 INFO @ Mon, 12 Aug 2019 21:45:41: #1 tag size is determined as 51 bps INFO @ Mon, 12 Aug 2019 21:45:41: #1 tag size = 51 INFO @ Mon, 12 Aug 2019 21:45:41: #1 total tags in treatment: 37087333 INFO @ Mon, 12 Aug 2019 21:45:41: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 21:45:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 21:45:42: #1 tags after filtering in treatment: 37087333 INFO @ Mon, 12 Aug 2019 21:45:42: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 21:45:42: #1 finished! INFO @ Mon, 12 Aug 2019 21:45:42: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 21:45:42: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 21:45:45: #2 number of paired peaks: 21 WARNING @ Mon, 12 Aug 2019 21:45:45: Too few paired peaks (21) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 21:45:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981637/SRX3981637.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981637/SRX3981637.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981637/SRX3981637.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981637/SRX3981637.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。