Job ID = 6528029 SRX = SRX3981635 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:08:52 prefetch.2.10.7: 1) Downloading 'SRR7050289'... 2020-06-29T14:08:52 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:10:36 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:10:36 prefetch.2.10.7: 'SRR7050289' is valid 2020-06-29T14:10:36 prefetch.2.10.7: 1) 'SRR7050289' was downloaded successfully 2020-06-29T14:10:36 prefetch.2.10.7: 'SRR7050289' has 0 unresolved dependencies Read 13895995 spots for SRR7050289/SRR7050289.sra Written 13895995 spots for SRR7050289/SRR7050289.sra 2020-06-29T14:11:33 prefetch.2.10.7: 1) Downloading 'SRR7050290'... 2020-06-29T14:11:33 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:12:46 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:12:46 prefetch.2.10.7: 'SRR7050290' is valid 2020-06-29T14:12:46 prefetch.2.10.7: 1) 'SRR7050290' was downloaded successfully 2020-06-29T14:12:46 prefetch.2.10.7: 'SRR7050290' has 0 unresolved dependencies Read 13790922 spots for SRR7050290/SRR7050290.sra Written 13790922 spots for SRR7050290/SRR7050290.sra 2020-06-29T14:13:43 prefetch.2.10.7: 1) Downloading 'SRR7050291'... 2020-06-29T14:13:43 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:15:01 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:15:01 prefetch.2.10.7: 'SRR7050291' is valid 2020-06-29T14:15:01 prefetch.2.10.7: 1) 'SRR7050291' was downloaded successfully 2020-06-29T14:15:01 prefetch.2.10.7: 'SRR7050291' has 0 unresolved dependencies Read 13771403 spots for SRR7050291/SRR7050291.sra Written 13771403 spots for SRR7050291/SRR7050291.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:01 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:12:19 41458320 reads; of these: 41458320 (100.00%) were unpaired; of these: 1190475 (2.87%) aligned 0 times 30859129 (74.43%) aligned exactly 1 time 9408716 (22.69%) aligned >1 times 97.13% overall alignment rate Time searching: 00:12:20 Overall time: 00:12:20 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 4539300 / 40267845 = 0.1127 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:47:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981635/SRX3981635.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981635/SRX3981635.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981635/SRX3981635.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981635/SRX3981635.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:47:22: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:47:22: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:47:29: 1000000 INFO @ Mon, 29 Jun 2020 23:47:35: 2000000 INFO @ Mon, 29 Jun 2020 23:47:41: 3000000 INFO @ Mon, 29 Jun 2020 23:47:48: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:47:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981635/SRX3981635.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981635/SRX3981635.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981635/SRX3981635.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981635/SRX3981635.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:47:52: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:47:52: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:47:54: 5000000 INFO @ Mon, 29 Jun 2020 23:47:59: 1000000 INFO @ Mon, 29 Jun 2020 23:48:01: 6000000 INFO @ Mon, 29 Jun 2020 23:48:06: 2000000 INFO @ Mon, 29 Jun 2020 23:48:08: 7000000 INFO @ Mon, 29 Jun 2020 23:48:13: 3000000 INFO @ Mon, 29 Jun 2020 23:48:15: 8000000 INFO @ Mon, 29 Jun 2020 23:48:20: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:48:22: 9000000 INFO @ Mon, 29 Jun 2020 23:48:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981635/SRX3981635.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981635/SRX3981635.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981635/SRX3981635.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981635/SRX3981635.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:48:22: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:48:22: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:48:27: 5000000 INFO @ Mon, 29 Jun 2020 23:48:29: 1000000 INFO @ Mon, 29 Jun 2020 23:48:29: 10000000 INFO @ Mon, 29 Jun 2020 23:48:34: 6000000 INFO @ Mon, 29 Jun 2020 23:48:35: 2000000 INFO @ Mon, 29 Jun 2020 23:48:36: 11000000 INFO @ Mon, 29 Jun 2020 23:48:40: 7000000 INFO @ Mon, 29 Jun 2020 23:48:41: 3000000 INFO @ Mon, 29 Jun 2020 23:48:43: 12000000 INFO @ Mon, 29 Jun 2020 23:48:47: 4000000 INFO @ Mon, 29 Jun 2020 23:48:47: 8000000 INFO @ Mon, 29 Jun 2020 23:48:50: 13000000 INFO @ Mon, 29 Jun 2020 23:48:54: 5000000 INFO @ Mon, 29 Jun 2020 23:48:54: 9000000 INFO @ Mon, 29 Jun 2020 23:48:57: 14000000 INFO @ Mon, 29 Jun 2020 23:49:00: 6000000 INFO @ Mon, 29 Jun 2020 23:49:01: 10000000 INFO @ Mon, 29 Jun 2020 23:49:04: 15000000 INFO @ Mon, 29 Jun 2020 23:49:06: 7000000 INFO @ Mon, 29 Jun 2020 23:49:08: 11000000 INFO @ Mon, 29 Jun 2020 23:49:11: 16000000 INFO @ Mon, 29 Jun 2020 23:49:12: 8000000 INFO @ Mon, 29 Jun 2020 23:49:15: 12000000 INFO @ Mon, 29 Jun 2020 23:49:18: 17000000 INFO @ Mon, 29 Jun 2020 23:49:19: 9000000 INFO @ Mon, 29 Jun 2020 23:49:22: 13000000 INFO @ Mon, 29 Jun 2020 23:49:25: 10000000 INFO @ Mon, 29 Jun 2020 23:49:25: 18000000 INFO @ Mon, 29 Jun 2020 23:49:29: 14000000 INFO @ Mon, 29 Jun 2020 23:49:31: 11000000 INFO @ Mon, 29 Jun 2020 23:49:32: 19000000 INFO @ Mon, 29 Jun 2020 23:49:36: 15000000 INFO @ Mon, 29 Jun 2020 23:49:38: 12000000 INFO @ Mon, 29 Jun 2020 23:49:39: 20000000 INFO @ Mon, 29 Jun 2020 23:49:43: 16000000 INFO @ Mon, 29 Jun 2020 23:49:44: 13000000 INFO @ Mon, 29 Jun 2020 23:49:46: 21000000 INFO @ Mon, 29 Jun 2020 23:49:50: 17000000 INFO @ Mon, 29 Jun 2020 23:49:50: 14000000 INFO @ Mon, 29 Jun 2020 23:49:53: 22000000 INFO @ Mon, 29 Jun 2020 23:49:57: 15000000 INFO @ Mon, 29 Jun 2020 23:49:57: 18000000 INFO @ Mon, 29 Jun 2020 23:50:00: 23000000 INFO @ Mon, 29 Jun 2020 23:50:03: 16000000 INFO @ Mon, 29 Jun 2020 23:50:04: 19000000 INFO @ Mon, 29 Jun 2020 23:50:07: 24000000 INFO @ Mon, 29 Jun 2020 23:50:09: 17000000 INFO @ Mon, 29 Jun 2020 23:50:10: 20000000 INFO @ Mon, 29 Jun 2020 23:50:14: 25000000 INFO @ Mon, 29 Jun 2020 23:50:16: 18000000 INFO @ Mon, 29 Jun 2020 23:50:17: 21000000 INFO @ Mon, 29 Jun 2020 23:50:21: 26000000 INFO @ Mon, 29 Jun 2020 23:50:22: 19000000 INFO @ Mon, 29 Jun 2020 23:50:24: 22000000 INFO @ Mon, 29 Jun 2020 23:50:27: 27000000 INFO @ Mon, 29 Jun 2020 23:50:28: 20000000 INFO @ Mon, 29 Jun 2020 23:50:31: 23000000 INFO @ Mon, 29 Jun 2020 23:50:34: 28000000 INFO @ Mon, 29 Jun 2020 23:50:35: 21000000 INFO @ Mon, 29 Jun 2020 23:50:38: 24000000 INFO @ Mon, 29 Jun 2020 23:50:41: 29000000 INFO @ Mon, 29 Jun 2020 23:50:41: 22000000 INFO @ Mon, 29 Jun 2020 23:50:45: 25000000 INFO @ Mon, 29 Jun 2020 23:50:48: 23000000 INFO @ Mon, 29 Jun 2020 23:50:48: 30000000 INFO @ Mon, 29 Jun 2020 23:50:52: 26000000 INFO @ Mon, 29 Jun 2020 23:50:54: 24000000 INFO @ Mon, 29 Jun 2020 23:50:55: 31000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 23:50:59: 27000000 INFO @ Mon, 29 Jun 2020 23:51:00: 25000000 INFO @ Mon, 29 Jun 2020 23:51:01: 32000000 INFO @ Mon, 29 Jun 2020 23:51:05: 28000000 INFO @ Mon, 29 Jun 2020 23:51:07: 26000000 INFO @ Mon, 29 Jun 2020 23:51:08: 33000000 INFO @ Mon, 29 Jun 2020 23:51:12: 29000000 INFO @ Mon, 29 Jun 2020 23:51:13: 27000000 INFO @ Mon, 29 Jun 2020 23:51:15: 34000000 INFO @ Mon, 29 Jun 2020 23:51:19: 30000000 INFO @ Mon, 29 Jun 2020 23:51:19: 28000000 INFO @ Mon, 29 Jun 2020 23:51:21: 35000000 INFO @ Mon, 29 Jun 2020 23:51:25: 31000000 INFO @ Mon, 29 Jun 2020 23:51:26: 29000000 INFO @ Mon, 29 Jun 2020 23:51:27: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:51:27: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:51:27: #1 total tags in treatment: 35728545 INFO @ Mon, 29 Jun 2020 23:51:27: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:51:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:51:27: #1 tags after filtering in treatment: 35728545 INFO @ Mon, 29 Jun 2020 23:51:27: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:51:27: #1 finished! INFO @ Mon, 29 Jun 2020 23:51:27: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:51:27: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:51:29: #2 number of paired peaks: 32 WARNING @ Mon, 29 Jun 2020 23:51:29: Too few paired peaks (32) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:51:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981635/SRX3981635.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981635/SRX3981635.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981635/SRX3981635.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981635/SRX3981635.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:51:32: 30000000 INFO @ Mon, 29 Jun 2020 23:51:32: 32000000 INFO @ Mon, 29 Jun 2020 23:51:38: 31000000 INFO @ Mon, 29 Jun 2020 23:51:39: 33000000 INFO @ Mon, 29 Jun 2020 23:51:45: 32000000 INFO @ Mon, 29 Jun 2020 23:51:46: 34000000 INFO @ Mon, 29 Jun 2020 23:51:51: 33000000 INFO @ Mon, 29 Jun 2020 23:51:52: 35000000 INFO @ Mon, 29 Jun 2020 23:51:57: 34000000 INFO @ Mon, 29 Jun 2020 23:51:58: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:51:58: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:51:58: #1 total tags in treatment: 35728545 INFO @ Mon, 29 Jun 2020 23:51:58: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:51:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:51:58: #1 tags after filtering in treatment: 35728545 INFO @ Mon, 29 Jun 2020 23:51:58: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:51:58: #1 finished! INFO @ Mon, 29 Jun 2020 23:51:58: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:51:58: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:52:01: #2 number of paired peaks: 32 WARNING @ Mon, 29 Jun 2020 23:52:01: Too few paired peaks (32) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:52:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981635/SRX3981635.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981635/SRX3981635.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981635/SRX3981635.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981635/SRX3981635.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:52:03: 35000000 INFO @ Mon, 29 Jun 2020 23:52:07: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:52:07: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:52:07: #1 total tags in treatment: 35728545 INFO @ Mon, 29 Jun 2020 23:52:07: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:52:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:52:08: #1 tags after filtering in treatment: 35728545 INFO @ Mon, 29 Jun 2020 23:52:08: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:52:08: #1 finished! INFO @ Mon, 29 Jun 2020 23:52:08: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:52:08: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:52:10: #2 number of paired peaks: 32 WARNING @ Mon, 29 Jun 2020 23:52:10: Too few paired peaks (32) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:52:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981635/SRX3981635.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981635/SRX3981635.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981635/SRX3981635.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981635/SRX3981635.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。