Job ID = 6528027 SRX = SRX3981633 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:20:52 prefetch.2.10.7: 1) Downloading 'SRR7050283'... 2020-06-29T14:20:52 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:22:03 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:22:03 prefetch.2.10.7: 'SRR7050283' is valid 2020-06-29T14:22:03 prefetch.2.10.7: 1) 'SRR7050283' was downloaded successfully 2020-06-29T14:22:03 prefetch.2.10.7: 'SRR7050283' has 0 unresolved dependencies Read 13299287 spots for SRR7050283/SRR7050283.sra Written 13299287 spots for SRR7050283/SRR7050283.sra 2020-06-29T14:22:57 prefetch.2.10.7: 1) Downloading 'SRR7050284'... 2020-06-29T14:22:57 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:23:58 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:23:59 prefetch.2.10.7: 'SRR7050284' is valid 2020-06-29T14:23:59 prefetch.2.10.7: 1) 'SRR7050284' was downloaded successfully 2020-06-29T14:23:59 prefetch.2.10.7: 'SRR7050284' has 0 unresolved dependencies Read 13198634 spots for SRR7050284/SRR7050284.sra Written 13198634 spots for SRR7050284/SRR7050284.sra 2020-06-29T14:24:53 prefetch.2.10.7: 1) Downloading 'SRR7050285'... 2020-06-29T14:24:53 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:26:21 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:26:22 prefetch.2.10.7: 'SRR7050285' is valid 2020-06-29T14:26:22 prefetch.2.10.7: 1) 'SRR7050285' was downloaded successfully 2020-06-29T14:26:22 prefetch.2.10.7: 'SRR7050285' has 0 unresolved dependencies Read 13172705 spots for SRR7050285/SRR7050285.sra Written 13172705 spots for SRR7050285/SRR7050285.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:20 39670626 reads; of these: 39670626 (100.00%) were unpaired; of these: 972323 (2.45%) aligned 0 times 30145188 (75.99%) aligned exactly 1 time 8553115 (21.56%) aligned >1 times 97.55% overall alignment rate Time searching: 00:11:20 Overall time: 00:11:20 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 4610972 / 38698303 = 0.1192 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:55:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981633/SRX3981633.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981633/SRX3981633.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981633/SRX3981633.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981633/SRX3981633.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:55:33: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:55:33: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:55:38: 1000000 INFO @ Mon, 29 Jun 2020 23:55:43: 2000000 INFO @ Mon, 29 Jun 2020 23:55:47: 3000000 INFO @ Mon, 29 Jun 2020 23:55:53: 4000000 INFO @ Mon, 29 Jun 2020 23:55:58: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:56:03: 6000000 INFO @ Mon, 29 Jun 2020 23:56:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981633/SRX3981633.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981633/SRX3981633.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981633/SRX3981633.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981633/SRX3981633.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:56:03: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:56:03: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:56:08: 7000000 INFO @ Mon, 29 Jun 2020 23:56:09: 1000000 INFO @ Mon, 29 Jun 2020 23:56:14: 8000000 INFO @ Mon, 29 Jun 2020 23:56:14: 2000000 INFO @ Mon, 29 Jun 2020 23:56:19: 9000000 INFO @ Mon, 29 Jun 2020 23:56:20: 3000000 INFO @ Mon, 29 Jun 2020 23:56:24: 10000000 INFO @ Mon, 29 Jun 2020 23:56:25: 4000000 INFO @ Mon, 29 Jun 2020 23:56:30: 11000000 INFO @ Mon, 29 Jun 2020 23:56:30: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:56:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981633/SRX3981633.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981633/SRX3981633.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981633/SRX3981633.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981633/SRX3981633.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:56:33: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:56:33: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:56:35: 12000000 INFO @ Mon, 29 Jun 2020 23:56:36: 6000000 INFO @ Mon, 29 Jun 2020 23:56:38: 1000000 INFO @ Mon, 29 Jun 2020 23:56:41: 13000000 INFO @ Mon, 29 Jun 2020 23:56:41: 7000000 INFO @ Mon, 29 Jun 2020 23:56:42: 2000000 INFO @ Mon, 29 Jun 2020 23:56:46: 14000000 INFO @ Mon, 29 Jun 2020 23:56:47: 8000000 INFO @ Mon, 29 Jun 2020 23:56:47: 3000000 INFO @ Mon, 29 Jun 2020 23:56:51: 15000000 INFO @ Mon, 29 Jun 2020 23:56:51: 4000000 INFO @ Mon, 29 Jun 2020 23:56:52: 9000000 INFO @ Mon, 29 Jun 2020 23:56:56: 5000000 INFO @ Mon, 29 Jun 2020 23:56:57: 16000000 INFO @ Mon, 29 Jun 2020 23:56:57: 10000000 INFO @ Mon, 29 Jun 2020 23:57:00: 6000000 INFO @ Mon, 29 Jun 2020 23:57:02: 17000000 INFO @ Mon, 29 Jun 2020 23:57:03: 11000000 INFO @ Mon, 29 Jun 2020 23:57:05: 7000000 INFO @ Mon, 29 Jun 2020 23:57:07: 18000000 INFO @ Mon, 29 Jun 2020 23:57:08: 12000000 INFO @ Mon, 29 Jun 2020 23:57:09: 8000000 INFO @ Mon, 29 Jun 2020 23:57:12: 19000000 INFO @ Mon, 29 Jun 2020 23:57:13: 13000000 INFO @ Mon, 29 Jun 2020 23:57:14: 9000000 INFO @ Mon, 29 Jun 2020 23:57:18: 20000000 INFO @ Mon, 29 Jun 2020 23:57:18: 14000000 INFO @ Mon, 29 Jun 2020 23:57:18: 10000000 INFO @ Mon, 29 Jun 2020 23:57:23: 21000000 INFO @ Mon, 29 Jun 2020 23:57:23: 11000000 INFO @ Mon, 29 Jun 2020 23:57:24: 15000000 INFO @ Mon, 29 Jun 2020 23:57:28: 12000000 INFO @ Mon, 29 Jun 2020 23:57:28: 22000000 INFO @ Mon, 29 Jun 2020 23:57:29: 16000000 INFO @ Mon, 29 Jun 2020 23:57:32: 13000000 INFO @ Mon, 29 Jun 2020 23:57:33: 23000000 INFO @ Mon, 29 Jun 2020 23:57:34: 17000000 INFO @ Mon, 29 Jun 2020 23:57:37: 14000000 INFO @ Mon, 29 Jun 2020 23:57:39: 24000000 INFO @ Mon, 29 Jun 2020 23:57:40: 18000000 INFO @ Mon, 29 Jun 2020 23:57:41: 15000000 INFO @ Mon, 29 Jun 2020 23:57:44: 25000000 INFO @ Mon, 29 Jun 2020 23:57:45: 19000000 INFO @ Mon, 29 Jun 2020 23:57:46: 16000000 INFO @ Mon, 29 Jun 2020 23:57:49: 26000000 INFO @ Mon, 29 Jun 2020 23:57:50: 20000000 INFO @ Mon, 29 Jun 2020 23:57:50: 17000000 INFO @ Mon, 29 Jun 2020 23:57:55: 27000000 INFO @ Mon, 29 Jun 2020 23:57:55: 18000000 INFO @ Mon, 29 Jun 2020 23:57:56: 21000000 INFO @ Mon, 29 Jun 2020 23:58:00: 19000000 INFO @ Mon, 29 Jun 2020 23:58:00: 28000000 INFO @ Mon, 29 Jun 2020 23:58:01: 22000000 INFO @ Mon, 29 Jun 2020 23:58:04: 20000000 INFO @ Mon, 29 Jun 2020 23:58:05: 29000000 INFO @ Mon, 29 Jun 2020 23:58:06: 23000000 INFO @ Mon, 29 Jun 2020 23:58:09: 21000000 INFO @ Mon, 29 Jun 2020 23:58:11: 30000000 INFO @ Mon, 29 Jun 2020 23:58:11: 24000000 INFO @ Mon, 29 Jun 2020 23:58:13: 22000000 INFO @ Mon, 29 Jun 2020 23:58:15: 31000000 INFO @ Mon, 29 Jun 2020 23:58:17: 25000000 INFO @ Mon, 29 Jun 2020 23:58:18: 23000000 INFO @ Mon, 29 Jun 2020 23:58:20: 32000000 INFO @ Mon, 29 Jun 2020 23:58:22: 26000000 INFO @ Mon, 29 Jun 2020 23:58:23: 24000000 INFO @ Mon, 29 Jun 2020 23:58:25: 33000000 INFO @ Mon, 29 Jun 2020 23:58:27: 25000000 INFO @ Mon, 29 Jun 2020 23:58:27: 27000000 INFO @ Mon, 29 Jun 2020 23:58:30: 34000000 INFO @ Mon, 29 Jun 2020 23:58:31: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:58:31: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:58:31: #1 total tags in treatment: 34087331 INFO @ Mon, 29 Jun 2020 23:58:31: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:58:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:58:31: #1 tags after filtering in treatment: 34087331 INFO @ Mon, 29 Jun 2020 23:58:31: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:58:31: #1 finished! INFO @ Mon, 29 Jun 2020 23:58:31: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:58:31: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:58:32: 26000000 INFO @ Mon, 29 Jun 2020 23:58:32: 28000000 INFO @ Mon, 29 Jun 2020 23:58:34: #2 number of paired peaks: 80 WARNING @ Mon, 29 Jun 2020 23:58:34: Too few paired peaks (80) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:58:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981633/SRX3981633.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981633/SRX3981633.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981633/SRX3981633.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981633/SRX3981633.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:58:36: 27000000 INFO @ Mon, 29 Jun 2020 23:58:38: 29000000 INFO @ Mon, 29 Jun 2020 23:58:41: 28000000 INFO @ Mon, 29 Jun 2020 23:58:43: 30000000 INFO @ Mon, 29 Jun 2020 23:58:45: 29000000 INFO @ Mon, 29 Jun 2020 23:58:47: 31000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 23:58:50: 30000000 INFO @ Mon, 29 Jun 2020 23:58:52: 32000000 INFO @ Mon, 29 Jun 2020 23:58:56: 31000000 INFO @ Mon, 29 Jun 2020 23:58:57: 33000000 INFO @ Mon, 29 Jun 2020 23:59:01: 32000000 INFO @ Mon, 29 Jun 2020 23:59:02: 34000000 INFO @ Mon, 29 Jun 2020 23:59:03: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:59:03: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:59:03: #1 total tags in treatment: 34087331 INFO @ Mon, 29 Jun 2020 23:59:03: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:59:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:59:04: #1 tags after filtering in treatment: 34087331 INFO @ Mon, 29 Jun 2020 23:59:04: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:59:04: #1 finished! INFO @ Mon, 29 Jun 2020 23:59:04: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:59:04: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:59:06: #2 number of paired peaks: 80 WARNING @ Mon, 29 Jun 2020 23:59:06: Too few paired peaks (80) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:59:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981633/SRX3981633.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981633/SRX3981633.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981633/SRX3981633.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981633/SRX3981633.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:59:06: 33000000 INFO @ Mon, 29 Jun 2020 23:59:10: 34000000 INFO @ Mon, 29 Jun 2020 23:59:11: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:59:11: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:59:11: #1 total tags in treatment: 34087331 INFO @ Mon, 29 Jun 2020 23:59:11: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:59:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:59:12: #1 tags after filtering in treatment: 34087331 INFO @ Mon, 29 Jun 2020 23:59:12: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:59:12: #1 finished! INFO @ Mon, 29 Jun 2020 23:59:12: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:59:12: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:59:14: #2 number of paired peaks: 80 WARNING @ Mon, 29 Jun 2020 23:59:14: Too few paired peaks (80) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:59:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981633/SRX3981633.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981633/SRX3981633.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981633/SRX3981633.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981633/SRX3981633.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。