Job ID = 6528025 SRX = SRX3981632 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:00:36 prefetch.2.10.7: 1) Downloading 'SRR7050280'... 2020-06-29T14:00:36 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:02:06 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:02:06 prefetch.2.10.7: 'SRR7050280' is valid 2020-06-29T14:02:06 prefetch.2.10.7: 1) 'SRR7050280' was downloaded successfully 2020-06-29T14:02:06 prefetch.2.10.7: 'SRR7050280' has 0 unresolved dependencies Read 13823635 spots for SRR7050280/SRR7050280.sra Written 13823635 spots for SRR7050280/SRR7050280.sra 2020-06-29T14:03:03 prefetch.2.10.7: 1) Downloading 'SRR7050281'... 2020-06-29T14:03:03 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:05:19 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:05:20 prefetch.2.10.7: 'SRR7050281' is valid 2020-06-29T14:05:20 prefetch.2.10.7: 1) 'SRR7050281' was downloaded successfully 2020-06-29T14:05:20 prefetch.2.10.7: 'SRR7050281' has 0 unresolved dependencies Read 13715388 spots for SRR7050281/SRR7050281.sra Written 13715388 spots for SRR7050281/SRR7050281.sra 2020-06-29T14:06:16 prefetch.2.10.7: 1) Downloading 'SRR7050282'... 2020-06-29T14:06:16 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:07:56 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:07:57 prefetch.2.10.7: 'SRR7050282' is valid 2020-06-29T14:07:57 prefetch.2.10.7: 1) 'SRR7050282' was downloaded successfully 2020-06-29T14:07:57 prefetch.2.10.7: 'SRR7050282' has 0 unresolved dependencies Read 13697910 spots for SRR7050282/SRR7050282.sra Written 13697910 spots for SRR7050282/SRR7050282.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 00:12:05 41236933 reads; of these: 41236933 (100.00%) were unpaired; of these: 1242910 (3.01%) aligned 0 times 29960572 (72.65%) aligned exactly 1 time 10033451 (24.33%) aligned >1 times 96.99% overall alignment rate Time searching: 00:12:06 Overall time: 00:12:06 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 4515128 / 39994023 = 0.1129 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:38:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981632/SRX3981632.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981632/SRX3981632.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981632/SRX3981632.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981632/SRX3981632.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:38:17: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:38:17: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:38:22: 1000000 INFO @ Mon, 29 Jun 2020 23:38:27: 2000000 INFO @ Mon, 29 Jun 2020 23:38:32: 3000000 INFO @ Mon, 29 Jun 2020 23:38:37: 4000000 INFO @ Mon, 29 Jun 2020 23:38:42: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:38:47: 6000000 INFO @ Mon, 29 Jun 2020 23:38:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981632/SRX3981632.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981632/SRX3981632.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981632/SRX3981632.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981632/SRX3981632.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:38:47: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:38:47: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:38:52: 7000000 INFO @ Mon, 29 Jun 2020 23:38:52: 1000000 INFO @ Mon, 29 Jun 2020 23:38:57: 8000000 INFO @ Mon, 29 Jun 2020 23:38:58: 2000000 INFO @ Mon, 29 Jun 2020 23:39:03: 9000000 INFO @ Mon, 29 Jun 2020 23:39:03: 3000000 INFO @ Mon, 29 Jun 2020 23:39:08: 10000000 INFO @ Mon, 29 Jun 2020 23:39:08: 4000000 INFO @ Mon, 29 Jun 2020 23:39:13: 11000000 INFO @ Mon, 29 Jun 2020 23:39:14: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:39:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981632/SRX3981632.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981632/SRX3981632.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981632/SRX3981632.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981632/SRX3981632.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:39:17: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:39:17: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:39:19: 12000000 INFO @ Mon, 29 Jun 2020 23:39:19: 6000000 INFO @ Mon, 29 Jun 2020 23:39:22: 1000000 INFO @ Mon, 29 Jun 2020 23:39:24: 13000000 INFO @ Mon, 29 Jun 2020 23:39:25: 7000000 INFO @ Mon, 29 Jun 2020 23:39:28: 2000000 INFO @ Mon, 29 Jun 2020 23:39:29: 14000000 INFO @ Mon, 29 Jun 2020 23:39:30: 8000000 INFO @ Mon, 29 Jun 2020 23:39:33: 3000000 INFO @ Mon, 29 Jun 2020 23:39:35: 15000000 INFO @ Mon, 29 Jun 2020 23:39:35: 9000000 INFO @ Mon, 29 Jun 2020 23:39:39: 4000000 INFO @ Mon, 29 Jun 2020 23:39:40: 16000000 INFO @ Mon, 29 Jun 2020 23:39:41: 10000000 INFO @ Mon, 29 Jun 2020 23:39:44: 5000000 INFO @ Mon, 29 Jun 2020 23:39:45: 17000000 INFO @ Mon, 29 Jun 2020 23:39:46: 11000000 INFO @ Mon, 29 Jun 2020 23:39:49: 6000000 INFO @ Mon, 29 Jun 2020 23:39:50: 18000000 INFO @ Mon, 29 Jun 2020 23:39:51: 12000000 INFO @ Mon, 29 Jun 2020 23:39:55: 7000000 INFO @ Mon, 29 Jun 2020 23:39:56: 19000000 INFO @ Mon, 29 Jun 2020 23:39:57: 13000000 INFO @ Mon, 29 Jun 2020 23:40:00: 8000000 INFO @ Mon, 29 Jun 2020 23:40:01: 20000000 INFO @ Mon, 29 Jun 2020 23:40:02: 14000000 INFO @ Mon, 29 Jun 2020 23:40:05: 9000000 INFO @ Mon, 29 Jun 2020 23:40:06: 21000000 INFO @ Mon, 29 Jun 2020 23:40:07: 15000000 INFO @ Mon, 29 Jun 2020 23:40:11: 10000000 INFO @ Mon, 29 Jun 2020 23:40:11: 22000000 INFO @ Mon, 29 Jun 2020 23:40:12: 16000000 INFO @ Mon, 29 Jun 2020 23:40:16: 11000000 INFO @ Mon, 29 Jun 2020 23:40:16: 23000000 INFO @ Mon, 29 Jun 2020 23:40:18: 17000000 INFO @ Mon, 29 Jun 2020 23:40:21: 12000000 INFO @ Mon, 29 Jun 2020 23:40:22: 24000000 INFO @ Mon, 29 Jun 2020 23:40:23: 18000000 INFO @ Mon, 29 Jun 2020 23:40:26: 13000000 INFO @ Mon, 29 Jun 2020 23:40:27: 25000000 INFO @ Mon, 29 Jun 2020 23:40:28: 19000000 INFO @ Mon, 29 Jun 2020 23:40:32: 14000000 INFO @ Mon, 29 Jun 2020 23:40:33: 26000000 INFO @ Mon, 29 Jun 2020 23:40:33: 20000000 INFO @ Mon, 29 Jun 2020 23:40:37: 15000000 INFO @ Mon, 29 Jun 2020 23:40:38: 27000000 INFO @ Mon, 29 Jun 2020 23:40:38: 21000000 INFO @ Mon, 29 Jun 2020 23:40:42: 16000000 INFO @ Mon, 29 Jun 2020 23:40:43: 28000000 INFO @ Mon, 29 Jun 2020 23:40:43: 22000000 INFO @ Mon, 29 Jun 2020 23:40:47: 17000000 INFO @ Mon, 29 Jun 2020 23:40:49: 29000000 INFO @ Mon, 29 Jun 2020 23:40:49: 23000000 INFO @ Mon, 29 Jun 2020 23:40:52: 18000000 INFO @ Mon, 29 Jun 2020 23:40:54: 30000000 INFO @ Mon, 29 Jun 2020 23:40:54: 24000000 INFO @ Mon, 29 Jun 2020 23:40:57: 19000000 INFO @ Mon, 29 Jun 2020 23:40:59: 31000000 INFO @ Mon, 29 Jun 2020 23:40:59: 25000000 INFO @ Mon, 29 Jun 2020 23:41:02: 20000000 INFO @ Mon, 29 Jun 2020 23:41:04: 32000000 INFO @ Mon, 29 Jun 2020 23:41:05: 26000000 INFO @ Mon, 29 Jun 2020 23:41:07: 21000000 INFO @ Mon, 29 Jun 2020 23:41:09: 33000000 INFO @ Mon, 29 Jun 2020 23:41:10: 27000000 INFO @ Mon, 29 Jun 2020 23:41:12: 22000000 INFO @ Mon, 29 Jun 2020 23:41:15: 34000000 INFO @ Mon, 29 Jun 2020 23:41:15: 28000000 INFO @ Mon, 29 Jun 2020 23:41:18: 23000000 INFO @ Mon, 29 Jun 2020 23:41:20: 35000000 INFO @ Mon, 29 Jun 2020 23:41:20: 29000000 INFO @ Mon, 29 Jun 2020 23:41:23: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:41:23: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:41:23: #1 total tags in treatment: 35478895 INFO @ Mon, 29 Jun 2020 23:41:23: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:41:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:41:23: 24000000 INFO @ Mon, 29 Jun 2020 23:41:23: #1 tags after filtering in treatment: 35478895 INFO @ Mon, 29 Jun 2020 23:41:23: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:41:23: #1 finished! INFO @ Mon, 29 Jun 2020 23:41:23: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:41:23: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:41:26: #2 number of paired peaks: 40 WARNING @ Mon, 29 Jun 2020 23:41:26: Too few paired peaks (40) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:41:26: Process for pairing-model is terminated! INFO @ Mon, 29 Jun 2020 23:41:26: 30000000 cut: /home/okishinya/chipatlas/results/dm3/SRX3981632/SRX3981632.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981632/SRX3981632.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981632/SRX3981632.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981632/SRX3981632.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:41:28: 25000000 INFO @ Mon, 29 Jun 2020 23:41:31: 31000000 INFO @ Mon, 29 Jun 2020 23:41:33: 26000000 INFO @ Mon, 29 Jun 2020 23:41:36: 32000000 INFO @ Mon, 29 Jun 2020 23:41:38: 27000000 INFO @ Mon, 29 Jun 2020 23:41:41: 33000000 INFO @ Mon, 29 Jun 2020 23:41:43: 28000000 INFO @ Mon, 29 Jun 2020 23:41:46: 34000000 INFO @ Mon, 29 Jun 2020 23:41:49: 29000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 23:41:51: 35000000 INFO @ Mon, 29 Jun 2020 23:41:54: 30000000 INFO @ Mon, 29 Jun 2020 23:41:54: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:41:54: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:41:54: #1 total tags in treatment: 35478895 INFO @ Mon, 29 Jun 2020 23:41:54: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:41:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:41:54: #1 tags after filtering in treatment: 35478895 INFO @ Mon, 29 Jun 2020 23:41:54: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:41:54: #1 finished! INFO @ Mon, 29 Jun 2020 23:41:54: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:41:54: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:41:57: #2 number of paired peaks: 40 WARNING @ Mon, 29 Jun 2020 23:41:57: Too few paired peaks (40) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:41:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981632/SRX3981632.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981632/SRX3981632.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981632/SRX3981632.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981632/SRX3981632.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:41:59: 31000000 INFO @ Mon, 29 Jun 2020 23:42:04: 32000000 INFO @ Mon, 29 Jun 2020 23:42:09: 33000000 INFO @ Mon, 29 Jun 2020 23:42:14: 34000000 INFO @ Mon, 29 Jun 2020 23:42:18: 35000000 INFO @ Mon, 29 Jun 2020 23:42:21: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:42:21: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:42:21: #1 total tags in treatment: 35478895 INFO @ Mon, 29 Jun 2020 23:42:21: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:42:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:42:22: #1 tags after filtering in treatment: 35478895 INFO @ Mon, 29 Jun 2020 23:42:22: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:42:22: #1 finished! INFO @ Mon, 29 Jun 2020 23:42:22: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:42:22: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:42:24: #2 number of paired peaks: 40 WARNING @ Mon, 29 Jun 2020 23:42:24: Too few paired peaks (40) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:42:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981632/SRX3981632.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981632/SRX3981632.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981632/SRX3981632.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981632/SRX3981632.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。