Job ID = 6528023 SRX = SRX3981630 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:06:20 prefetch.2.10.7: 1) Downloading 'SRR7050274'... 2020-06-29T14:06:20 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:07:49 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:07:50 prefetch.2.10.7: 'SRR7050274' is valid 2020-06-29T14:07:50 prefetch.2.10.7: 1) 'SRR7050274' was downloaded successfully 2020-06-29T14:07:50 prefetch.2.10.7: 'SRR7050274' has 0 unresolved dependencies Read 12738474 spots for SRR7050274/SRR7050274.sra Written 12738474 spots for SRR7050274/SRR7050274.sra 2020-06-29T14:08:45 prefetch.2.10.7: 1) Downloading 'SRR7050275'... 2020-06-29T14:08:45 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:10:25 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:10:25 prefetch.2.10.7: 'SRR7050275' is valid 2020-06-29T14:10:25 prefetch.2.10.7: 1) 'SRR7050275' was downloaded successfully 2020-06-29T14:10:25 prefetch.2.10.7: 'SRR7050275' has 0 unresolved dependencies Read 12628386 spots for SRR7050275/SRR7050275.sra Written 12628386 spots for SRR7050275/SRR7050275.sra 2020-06-29T14:11:18 prefetch.2.10.7: 1) Downloading 'SRR7050276'... 2020-06-29T14:11:18 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:12:36 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:12:36 prefetch.2.10.7: 'SRR7050276' is valid 2020-06-29T14:12:36 prefetch.2.10.7: 1) 'SRR7050276' was downloaded successfully 2020-06-29T14:12:36 prefetch.2.10.7: 'SRR7050276' has 0 unresolved dependencies Read 12597632 spots for SRR7050276/SRR7050276.sra Written 12597632 spots for SRR7050276/SRR7050276.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:41 37964492 reads; of these: 37964492 (100.00%) were unpaired; of these: 1143150 (3.01%) aligned 0 times 27521881 (72.49%) aligned exactly 1 time 9299461 (24.50%) aligned >1 times 96.99% overall alignment rate Time searching: 00:11:41 Overall time: 00:11:42 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 3837388 / 36821342 = 0.1042 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:42:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981630/SRX3981630.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981630/SRX3981630.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981630/SRX3981630.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981630/SRX3981630.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:42:34: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:42:34: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:42:40: 1000000 INFO @ Mon, 29 Jun 2020 23:42:45: 2000000 INFO @ Mon, 29 Jun 2020 23:42:51: 3000000 INFO @ Mon, 29 Jun 2020 23:42:56: 4000000 INFO @ Mon, 29 Jun 2020 23:43:01: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:43:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981630/SRX3981630.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981630/SRX3981630.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981630/SRX3981630.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981630/SRX3981630.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:43:05: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:43:05: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:43:07: 6000000 INFO @ Mon, 29 Jun 2020 23:43:11: 1000000 INFO @ Mon, 29 Jun 2020 23:43:13: 7000000 INFO @ Mon, 29 Jun 2020 23:43:17: 2000000 INFO @ Mon, 29 Jun 2020 23:43:19: 8000000 INFO @ Mon, 29 Jun 2020 23:43:23: 3000000 INFO @ Mon, 29 Jun 2020 23:43:24: 9000000 INFO @ Mon, 29 Jun 2020 23:43:29: 4000000 INFO @ Mon, 29 Jun 2020 23:43:30: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:43:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981630/SRX3981630.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981630/SRX3981630.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981630/SRX3981630.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981630/SRX3981630.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:43:35: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:43:35: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:43:36: 5000000 INFO @ Mon, 29 Jun 2020 23:43:36: 11000000 INFO @ Mon, 29 Jun 2020 23:43:41: 1000000 INFO @ Mon, 29 Jun 2020 23:43:42: 6000000 INFO @ Mon, 29 Jun 2020 23:43:42: 12000000 INFO @ Mon, 29 Jun 2020 23:43:47: 2000000 INFO @ Mon, 29 Jun 2020 23:43:48: 13000000 INFO @ Mon, 29 Jun 2020 23:43:48: 7000000 INFO @ Mon, 29 Jun 2020 23:43:53: 3000000 INFO @ Mon, 29 Jun 2020 23:43:54: 14000000 INFO @ Mon, 29 Jun 2020 23:43:54: 8000000 INFO @ Mon, 29 Jun 2020 23:43:58: 4000000 INFO @ Mon, 29 Jun 2020 23:44:00: 15000000 INFO @ Mon, 29 Jun 2020 23:44:00: 9000000 INFO @ Mon, 29 Jun 2020 23:44:04: 5000000 INFO @ Mon, 29 Jun 2020 23:44:05: 16000000 INFO @ Mon, 29 Jun 2020 23:44:06: 10000000 INFO @ Mon, 29 Jun 2020 23:44:11: 6000000 INFO @ Mon, 29 Jun 2020 23:44:11: 17000000 INFO @ Mon, 29 Jun 2020 23:44:13: 11000000 INFO @ Mon, 29 Jun 2020 23:44:17: 7000000 INFO @ Mon, 29 Jun 2020 23:44:18: 18000000 INFO @ Mon, 29 Jun 2020 23:44:19: 12000000 INFO @ Mon, 29 Jun 2020 23:44:23: 8000000 INFO @ Mon, 29 Jun 2020 23:44:24: 19000000 INFO @ Mon, 29 Jun 2020 23:44:25: 13000000 INFO @ Mon, 29 Jun 2020 23:44:29: 9000000 INFO @ Mon, 29 Jun 2020 23:44:29: 20000000 INFO @ Mon, 29 Jun 2020 23:44:31: 14000000 INFO @ Mon, 29 Jun 2020 23:44:35: 10000000 INFO @ Mon, 29 Jun 2020 23:44:35: 21000000 INFO @ Mon, 29 Jun 2020 23:44:37: 15000000 INFO @ Mon, 29 Jun 2020 23:44:41: 11000000 INFO @ Mon, 29 Jun 2020 23:44:41: 22000000 INFO @ Mon, 29 Jun 2020 23:44:43: 16000000 INFO @ Mon, 29 Jun 2020 23:44:47: 23000000 INFO @ Mon, 29 Jun 2020 23:44:47: 12000000 INFO @ Mon, 29 Jun 2020 23:44:49: 17000000 INFO @ Mon, 29 Jun 2020 23:44:53: 24000000 INFO @ Mon, 29 Jun 2020 23:44:53: 13000000 INFO @ Mon, 29 Jun 2020 23:44:55: 18000000 INFO @ Mon, 29 Jun 2020 23:44:59: 25000000 INFO @ Mon, 29 Jun 2020 23:44:59: 14000000 INFO @ Mon, 29 Jun 2020 23:45:01: 19000000 INFO @ Mon, 29 Jun 2020 23:45:05: 26000000 INFO @ Mon, 29 Jun 2020 23:45:05: 15000000 INFO @ Mon, 29 Jun 2020 23:45:07: 20000000 INFO @ Mon, 29 Jun 2020 23:45:10: 27000000 INFO @ Mon, 29 Jun 2020 23:45:11: 16000000 INFO @ Mon, 29 Jun 2020 23:45:13: 21000000 INFO @ Mon, 29 Jun 2020 23:45:16: 28000000 INFO @ Mon, 29 Jun 2020 23:45:17: 17000000 INFO @ Mon, 29 Jun 2020 23:45:19: 22000000 INFO @ Mon, 29 Jun 2020 23:45:22: 29000000 INFO @ Mon, 29 Jun 2020 23:45:22: 18000000 INFO @ Mon, 29 Jun 2020 23:45:25: 23000000 INFO @ Mon, 29 Jun 2020 23:45:28: 30000000 INFO @ Mon, 29 Jun 2020 23:45:28: 19000000 INFO @ Mon, 29 Jun 2020 23:45:31: 24000000 INFO @ Mon, 29 Jun 2020 23:45:34: 31000000 INFO @ Mon, 29 Jun 2020 23:45:34: 20000000 INFO @ Mon, 29 Jun 2020 23:45:37: 25000000 INFO @ Mon, 29 Jun 2020 23:45:40: 32000000 INFO @ Mon, 29 Jun 2020 23:45:40: 21000000 INFO @ Mon, 29 Jun 2020 23:45:42: 26000000 INFO @ Mon, 29 Jun 2020 23:45:46: 22000000 INFO @ Mon, 29 Jun 2020 23:45:46: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:45:46: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:45:46: #1 total tags in treatment: 32983954 INFO @ Mon, 29 Jun 2020 23:45:46: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:45:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:45:47: #1 tags after filtering in treatment: 32983954 INFO @ Mon, 29 Jun 2020 23:45:47: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:45:47: #1 finished! INFO @ Mon, 29 Jun 2020 23:45:47: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:45:47: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:45:48: 27000000 INFO @ Mon, 29 Jun 2020 23:45:49: #2 number of paired peaks: 29 WARNING @ Mon, 29 Jun 2020 23:45:49: Too few paired peaks (29) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:45:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981630/SRX3981630.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981630/SRX3981630.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981630/SRX3981630.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981630/SRX3981630.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:45:52: 23000000 INFO @ Mon, 29 Jun 2020 23:45:54: 28000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 23:45:58: 24000000 INFO @ Mon, 29 Jun 2020 23:46:00: 29000000 INFO @ Mon, 29 Jun 2020 23:46:04: 25000000 INFO @ Mon, 29 Jun 2020 23:46:06: 30000000 INFO @ Mon, 29 Jun 2020 23:46:09: 26000000 INFO @ Mon, 29 Jun 2020 23:46:12: 31000000 INFO @ Mon, 29 Jun 2020 23:46:15: 27000000 INFO @ Mon, 29 Jun 2020 23:46:18: 32000000 INFO @ Mon, 29 Jun 2020 23:46:21: 28000000 INFO @ Mon, 29 Jun 2020 23:46:24: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:46:24: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:46:24: #1 total tags in treatment: 32983954 INFO @ Mon, 29 Jun 2020 23:46:24: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:46:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:46:24: #1 tags after filtering in treatment: 32983954 INFO @ Mon, 29 Jun 2020 23:46:24: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:46:24: #1 finished! INFO @ Mon, 29 Jun 2020 23:46:24: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:46:24: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:46:26: #2 number of paired peaks: 29 WARNING @ Mon, 29 Jun 2020 23:46:26: Too few paired peaks (29) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:46:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981630/SRX3981630.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981630/SRX3981630.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981630/SRX3981630.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981630/SRX3981630.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:46:27: 29000000 INFO @ Mon, 29 Jun 2020 23:46:32: 30000000 INFO @ Mon, 29 Jun 2020 23:46:38: 31000000 INFO @ Mon, 29 Jun 2020 23:46:43: 32000000 INFO @ Mon, 29 Jun 2020 23:46:49: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:46:49: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:46:49: #1 total tags in treatment: 32983954 INFO @ Mon, 29 Jun 2020 23:46:49: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:46:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:46:49: #1 tags after filtering in treatment: 32983954 INFO @ Mon, 29 Jun 2020 23:46:49: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:46:49: #1 finished! INFO @ Mon, 29 Jun 2020 23:46:49: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:46:49: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:46:51: #2 number of paired peaks: 29 WARNING @ Mon, 29 Jun 2020 23:46:51: Too few paired peaks (29) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:46:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981630/SRX3981630.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981630/SRX3981630.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981630/SRX3981630.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981630/SRX3981630.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。