Job ID = 6528016 SRX = SRX3981614 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T13:49:30 prefetch.2.10.7: 1) Downloading 'SRR7050226'... 2020-06-29T13:49:30 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T13:50:48 prefetch.2.10.7: HTTPS download succeed 2020-06-29T13:50:48 prefetch.2.10.7: 'SRR7050226' is valid 2020-06-29T13:50:48 prefetch.2.10.7: 1) 'SRR7050226' was downloaded successfully 2020-06-29T13:50:48 prefetch.2.10.7: 'SRR7050226' has 0 unresolved dependencies Read 13425649 spots for SRR7050226/SRR7050226.sra Written 13425649 spots for SRR7050226/SRR7050226.sra 2020-06-29T13:51:40 prefetch.2.10.7: 1) Downloading 'SRR7050227'... 2020-06-29T13:51:40 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T13:52:58 prefetch.2.10.7: HTTPS download succeed 2020-06-29T13:52:58 prefetch.2.10.7: 'SRR7050227' is valid 2020-06-29T13:52:58 prefetch.2.10.7: 1) 'SRR7050227' was downloaded successfully 2020-06-29T13:52:58 prefetch.2.10.7: 'SRR7050227' has 0 unresolved dependencies Read 13173327 spots for SRR7050227/SRR7050227.sra Written 13173327 spots for SRR7050227/SRR7050227.sra 2020-06-29T13:53:52 prefetch.2.10.7: 1) Downloading 'SRR7050228'... 2020-06-29T13:53:52 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T13:55:18 prefetch.2.10.7: HTTPS download succeed 2020-06-29T13:55:18 prefetch.2.10.7: 'SRR7050228' is valid 2020-06-29T13:55:18 prefetch.2.10.7: 1) 'SRR7050228' was downloaded successfully 2020-06-29T13:55:18 prefetch.2.10.7: 'SRR7050228' has 0 unresolved dependencies Read 13409525 spots for SRR7050228/SRR7050228.sra Written 13409525 spots for SRR7050228/SRR7050228.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:42 40008501 reads; of these: 40008501 (100.00%) were unpaired; of these: 1165848 (2.91%) aligned 0 times 30066796 (75.15%) aligned exactly 1 time 8775857 (21.93%) aligned >1 times 97.09% overall alignment rate Time searching: 00:11:42 Overall time: 00:11:42 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 4498364 / 38842653 = 0.1158 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:24:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981614/SRX3981614.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981614/SRX3981614.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981614/SRX3981614.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981614/SRX3981614.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:24:46: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:24:46: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:24:52: 1000000 INFO @ Mon, 29 Jun 2020 23:24:57: 2000000 INFO @ Mon, 29 Jun 2020 23:25:03: 3000000 INFO @ Mon, 29 Jun 2020 23:25:08: 4000000 INFO @ Mon, 29 Jun 2020 23:25:14: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:25:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981614/SRX3981614.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981614/SRX3981614.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981614/SRX3981614.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981614/SRX3981614.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:25:16: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:25:16: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:25:20: 6000000 INFO @ Mon, 29 Jun 2020 23:25:22: 1000000 INFO @ Mon, 29 Jun 2020 23:25:26: 7000000 INFO @ Mon, 29 Jun 2020 23:25:28: 2000000 INFO @ Mon, 29 Jun 2020 23:25:32: 8000000 INFO @ Mon, 29 Jun 2020 23:25:34: 3000000 INFO @ Mon, 29 Jun 2020 23:25:39: 9000000 INFO @ Mon, 29 Jun 2020 23:25:40: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:25:45: 10000000 INFO @ Mon, 29 Jun 2020 23:25:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981614/SRX3981614.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981614/SRX3981614.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981614/SRX3981614.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981614/SRX3981614.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:25:46: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:25:46: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:25:47: 5000000 INFO @ Mon, 29 Jun 2020 23:25:51: 11000000 INFO @ Mon, 29 Jun 2020 23:25:52: 1000000 INFO @ Mon, 29 Jun 2020 23:25:53: 6000000 INFO @ Mon, 29 Jun 2020 23:25:57: 12000000 INFO @ Mon, 29 Jun 2020 23:25:58: 2000000 INFO @ Mon, 29 Jun 2020 23:25:59: 7000000 INFO @ Mon, 29 Jun 2020 23:26:04: 13000000 INFO @ Mon, 29 Jun 2020 23:26:05: 3000000 INFO @ Mon, 29 Jun 2020 23:26:05: 8000000 INFO @ Mon, 29 Jun 2020 23:26:10: 14000000 INFO @ Mon, 29 Jun 2020 23:26:11: 4000000 INFO @ Mon, 29 Jun 2020 23:26:11: 9000000 INFO @ Mon, 29 Jun 2020 23:26:16: 15000000 INFO @ Mon, 29 Jun 2020 23:26:17: 5000000 INFO @ Mon, 29 Jun 2020 23:26:17: 10000000 INFO @ Mon, 29 Jun 2020 23:26:22: 16000000 INFO @ Mon, 29 Jun 2020 23:26:23: 6000000 INFO @ Mon, 29 Jun 2020 23:26:24: 11000000 INFO @ Mon, 29 Jun 2020 23:26:28: 17000000 INFO @ Mon, 29 Jun 2020 23:26:30: 7000000 INFO @ Mon, 29 Jun 2020 23:26:30: 12000000 INFO @ Mon, 29 Jun 2020 23:26:35: 18000000 INFO @ Mon, 29 Jun 2020 23:26:36: 13000000 INFO @ Mon, 29 Jun 2020 23:26:36: 8000000 INFO @ Mon, 29 Jun 2020 23:26:41: 19000000 INFO @ Mon, 29 Jun 2020 23:26:42: 14000000 INFO @ Mon, 29 Jun 2020 23:26:42: 9000000 INFO @ Mon, 29 Jun 2020 23:26:47: 20000000 INFO @ Mon, 29 Jun 2020 23:26:48: 15000000 INFO @ Mon, 29 Jun 2020 23:26:48: 10000000 INFO @ Mon, 29 Jun 2020 23:26:53: 21000000 INFO @ Mon, 29 Jun 2020 23:26:54: 16000000 INFO @ Mon, 29 Jun 2020 23:26:54: 11000000 INFO @ Mon, 29 Jun 2020 23:26:59: 22000000 INFO @ Mon, 29 Jun 2020 23:27:00: 17000000 INFO @ Mon, 29 Jun 2020 23:27:01: 12000000 INFO @ Mon, 29 Jun 2020 23:27:05: 23000000 INFO @ Mon, 29 Jun 2020 23:27:06: 18000000 INFO @ Mon, 29 Jun 2020 23:27:07: 13000000 INFO @ Mon, 29 Jun 2020 23:27:11: 24000000 INFO @ Mon, 29 Jun 2020 23:27:12: 19000000 INFO @ Mon, 29 Jun 2020 23:27:13: 14000000 INFO @ Mon, 29 Jun 2020 23:27:17: 25000000 INFO @ Mon, 29 Jun 2020 23:27:18: 20000000 INFO @ Mon, 29 Jun 2020 23:27:19: 15000000 INFO @ Mon, 29 Jun 2020 23:27:24: 26000000 INFO @ Mon, 29 Jun 2020 23:27:24: 21000000 INFO @ Mon, 29 Jun 2020 23:27:25: 16000000 INFO @ Mon, 29 Jun 2020 23:27:30: 22000000 INFO @ Mon, 29 Jun 2020 23:27:30: 27000000 INFO @ Mon, 29 Jun 2020 23:27:31: 17000000 INFO @ Mon, 29 Jun 2020 23:27:36: 23000000 INFO @ Mon, 29 Jun 2020 23:27:36: 28000000 INFO @ Mon, 29 Jun 2020 23:27:37: 18000000 INFO @ Mon, 29 Jun 2020 23:27:42: 24000000 INFO @ Mon, 29 Jun 2020 23:27:42: 29000000 INFO @ Mon, 29 Jun 2020 23:27:43: 19000000 INFO @ Mon, 29 Jun 2020 23:27:48: 25000000 INFO @ Mon, 29 Jun 2020 23:27:48: 30000000 INFO @ Mon, 29 Jun 2020 23:27:49: 20000000 INFO @ Mon, 29 Jun 2020 23:27:53: 26000000 INFO @ Mon, 29 Jun 2020 23:27:55: 31000000 INFO @ Mon, 29 Jun 2020 23:27:55: 21000000 INFO @ Mon, 29 Jun 2020 23:27:59: 27000000 INFO @ Mon, 29 Jun 2020 23:28:01: 32000000 INFO @ Mon, 29 Jun 2020 23:28:01: 22000000 INFO @ Mon, 29 Jun 2020 23:28:05: 28000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 23:28:07: 33000000 INFO @ Mon, 29 Jun 2020 23:28:07: 23000000 INFO @ Mon, 29 Jun 2020 23:28:11: 29000000 INFO @ Mon, 29 Jun 2020 23:28:13: 34000000 INFO @ Mon, 29 Jun 2020 23:28:13: 24000000 INFO @ Mon, 29 Jun 2020 23:28:15: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:28:15: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:28:15: #1 total tags in treatment: 34344289 INFO @ Mon, 29 Jun 2020 23:28:15: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:28:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:28:16: #1 tags after filtering in treatment: 34344289 INFO @ Mon, 29 Jun 2020 23:28:16: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:28:16: #1 finished! INFO @ Mon, 29 Jun 2020 23:28:16: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:28:16: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:28:17: 30000000 INFO @ Mon, 29 Jun 2020 23:28:18: #2 number of paired peaks: 64 WARNING @ Mon, 29 Jun 2020 23:28:18: Too few paired peaks (64) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:28:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981614/SRX3981614.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981614/SRX3981614.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981614/SRX3981614.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981614/SRX3981614.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:28:19: 25000000 INFO @ Mon, 29 Jun 2020 23:28:23: 31000000 INFO @ Mon, 29 Jun 2020 23:28:25: 26000000 INFO @ Mon, 29 Jun 2020 23:28:29: 32000000 INFO @ Mon, 29 Jun 2020 23:28:31: 27000000 INFO @ Mon, 29 Jun 2020 23:28:35: 33000000 INFO @ Mon, 29 Jun 2020 23:28:37: 28000000 INFO @ Mon, 29 Jun 2020 23:28:41: 34000000 INFO @ Mon, 29 Jun 2020 23:28:43: 29000000 INFO @ Mon, 29 Jun 2020 23:28:44: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:28:44: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:28:44: #1 total tags in treatment: 34344289 INFO @ Mon, 29 Jun 2020 23:28:44: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:28:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:28:44: #1 tags after filtering in treatment: 34344289 INFO @ Mon, 29 Jun 2020 23:28:44: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:28:44: #1 finished! INFO @ Mon, 29 Jun 2020 23:28:44: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:28:44: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:28:46: #2 number of paired peaks: 64 WARNING @ Mon, 29 Jun 2020 23:28:46: Too few paired peaks (64) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:28:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981614/SRX3981614.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981614/SRX3981614.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981614/SRX3981614.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981614/SRX3981614.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:28:49: 30000000 INFO @ Mon, 29 Jun 2020 23:28:55: 31000000 INFO @ Mon, 29 Jun 2020 23:29:00: 32000000 INFO @ Mon, 29 Jun 2020 23:29:06: 33000000 INFO @ Mon, 29 Jun 2020 23:29:12: 34000000 INFO @ Mon, 29 Jun 2020 23:29:14: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:29:14: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:29:14: #1 total tags in treatment: 34344289 INFO @ Mon, 29 Jun 2020 23:29:14: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:29:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:29:14: #1 tags after filtering in treatment: 34344289 INFO @ Mon, 29 Jun 2020 23:29:14: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:29:14: #1 finished! INFO @ Mon, 29 Jun 2020 23:29:14: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:29:14: #2 looking for paired plus/minus strand peaks... BigWig に変換しました。 INFO @ Mon, 29 Jun 2020 23:29:16: #2 number of paired peaks: 64 WARNING @ Mon, 29 Jun 2020 23:29:16: Too few paired peaks (64) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:29:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981614/SRX3981614.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981614/SRX3981614.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981614/SRX3981614.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981614/SRX3981614.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling