Job ID = 6528012 SRX = SRX3981609 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:21:20 prefetch.2.10.7: 1) Downloading 'SRR7050211'... 2020-06-29T14:21:20 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:22:24 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:22:25 prefetch.2.10.7: 'SRR7050211' is valid 2020-06-29T14:22:25 prefetch.2.10.7: 1) 'SRR7050211' was downloaded successfully 2020-06-29T14:22:25 prefetch.2.10.7: 'SRR7050211' has 0 unresolved dependencies Read 12564162 spots for SRR7050211/SRR7050211.sra Written 12564162 spots for SRR7050211/SRR7050211.sra 2020-06-29T14:23:27 prefetch.2.10.7: 1) Downloading 'SRR7050212'... 2020-06-29T14:23:27 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:24:24 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:24:25 prefetch.2.10.7: 'SRR7050212' is valid 2020-06-29T14:24:25 prefetch.2.10.7: 1) 'SRR7050212' was downloaded successfully 2020-06-29T14:24:25 prefetch.2.10.7: 'SRR7050212' has 0 unresolved dependencies Read 12297950 spots for SRR7050212/SRR7050212.sra Written 12297950 spots for SRR7050212/SRR7050212.sra 2020-06-29T14:25:24 prefetch.2.10.7: 1) Downloading 'SRR7050213'... 2020-06-29T14:25:24 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:26:30 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:26:30 prefetch.2.10.7: 'SRR7050213' is valid 2020-06-29T14:26:30 prefetch.2.10.7: 1) 'SRR7050213' was downloaded successfully 2020-06-29T14:26:30 prefetch.2.10.7: 'SRR7050213' has 0 unresolved dependencies Read 12550225 spots for SRR7050213/SRR7050213.sra Written 12550225 spots for SRR7050213/SRR7050213.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:12 37412337 reads; of these: 37412337 (100.00%) were unpaired; of these: 936694 (2.50%) aligned 0 times 27901109 (74.58%) aligned exactly 1 time 8574534 (22.92%) aligned >1 times 97.50% overall alignment rate Time searching: 00:11:12 Overall time: 00:11:12 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 3983888 / 36475643 = 0.1092 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:59:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981609/SRX3981609.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981609/SRX3981609.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981609/SRX3981609.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981609/SRX3981609.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:59:45: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:59:45: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:59:50: 1000000 INFO @ Mon, 29 Jun 2020 23:59:55: 2000000 INFO @ Tue, 30 Jun 2020 00:00:00: 3000000 INFO @ Tue, 30 Jun 2020 00:00:05: 4000000 INFO @ Tue, 30 Jun 2020 00:00:10: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:00:15: 6000000 INFO @ Tue, 30 Jun 2020 00:00:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981609/SRX3981609.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981609/SRX3981609.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981609/SRX3981609.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981609/SRX3981609.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:00:15: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:00:15: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:00:20: 7000000 INFO @ Tue, 30 Jun 2020 00:00:21: 1000000 INFO @ Tue, 30 Jun 2020 00:00:26: 8000000 INFO @ Tue, 30 Jun 2020 00:00:27: 2000000 INFO @ Tue, 30 Jun 2020 00:00:31: 9000000 INFO @ Tue, 30 Jun 2020 00:00:34: 3000000 INFO @ Tue, 30 Jun 2020 00:00:35: 10000000 INFO @ Tue, 30 Jun 2020 00:00:40: 11000000 INFO @ Tue, 30 Jun 2020 00:00:41: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:00:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981609/SRX3981609.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981609/SRX3981609.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981609/SRX3981609.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981609/SRX3981609.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:00:45: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:00:45: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:00:46: 12000000 INFO @ Tue, 30 Jun 2020 00:00:47: 5000000 INFO @ Tue, 30 Jun 2020 00:00:50: 1000000 INFO @ Tue, 30 Jun 2020 00:00:52: 13000000 INFO @ Tue, 30 Jun 2020 00:00:54: 6000000 INFO @ Tue, 30 Jun 2020 00:00:56: 2000000 INFO @ Tue, 30 Jun 2020 00:00:58: 14000000 INFO @ Tue, 30 Jun 2020 00:01:00: 7000000 INFO @ Tue, 30 Jun 2020 00:01:02: 3000000 INFO @ Tue, 30 Jun 2020 00:01:03: 15000000 INFO @ Tue, 30 Jun 2020 00:01:06: 8000000 INFO @ Tue, 30 Jun 2020 00:01:08: 4000000 INFO @ Tue, 30 Jun 2020 00:01:09: 16000000 INFO @ Tue, 30 Jun 2020 00:01:12: 9000000 INFO @ Tue, 30 Jun 2020 00:01:14: 5000000 INFO @ Tue, 30 Jun 2020 00:01:15: 17000000 INFO @ Tue, 30 Jun 2020 00:01:18: 10000000 INFO @ Tue, 30 Jun 2020 00:01:20: 6000000 INFO @ Tue, 30 Jun 2020 00:01:20: 18000000 INFO @ Tue, 30 Jun 2020 00:01:25: 11000000 INFO @ Tue, 30 Jun 2020 00:01:26: 7000000 INFO @ Tue, 30 Jun 2020 00:01:26: 19000000 INFO @ Tue, 30 Jun 2020 00:01:31: 12000000 INFO @ Tue, 30 Jun 2020 00:01:31: 8000000 INFO @ Tue, 30 Jun 2020 00:01:32: 20000000 INFO @ Tue, 30 Jun 2020 00:01:37: 9000000 INFO @ Tue, 30 Jun 2020 00:01:37: 13000000 INFO @ Tue, 30 Jun 2020 00:01:37: 21000000 INFO @ Tue, 30 Jun 2020 00:01:43: 10000000 INFO @ Tue, 30 Jun 2020 00:01:43: 22000000 INFO @ Tue, 30 Jun 2020 00:01:43: 14000000 INFO @ Tue, 30 Jun 2020 00:01:48: 11000000 INFO @ Tue, 30 Jun 2020 00:01:49: 23000000 INFO @ Tue, 30 Jun 2020 00:01:50: 15000000 INFO @ Tue, 30 Jun 2020 00:01:54: 12000000 INFO @ Tue, 30 Jun 2020 00:01:54: 24000000 INFO @ Tue, 30 Jun 2020 00:01:56: 16000000 INFO @ Tue, 30 Jun 2020 00:02:00: 13000000 INFO @ Tue, 30 Jun 2020 00:02:00: 25000000 INFO @ Tue, 30 Jun 2020 00:02:02: 17000000 INFO @ Tue, 30 Jun 2020 00:02:06: 14000000 INFO @ Tue, 30 Jun 2020 00:02:06: 26000000 INFO @ Tue, 30 Jun 2020 00:02:08: 18000000 INFO @ Tue, 30 Jun 2020 00:02:12: 15000000 INFO @ Tue, 30 Jun 2020 00:02:12: 27000000 INFO @ Tue, 30 Jun 2020 00:02:14: 19000000 INFO @ Tue, 30 Jun 2020 00:02:18: 16000000 INFO @ Tue, 30 Jun 2020 00:02:18: 28000000 INFO @ Tue, 30 Jun 2020 00:02:20: 20000000 INFO @ Tue, 30 Jun 2020 00:02:23: 17000000 INFO @ Tue, 30 Jun 2020 00:02:24: 29000000 INFO @ Tue, 30 Jun 2020 00:02:26: 21000000 INFO @ Tue, 30 Jun 2020 00:02:29: 30000000 INFO @ Tue, 30 Jun 2020 00:02:30: 18000000 INFO @ Tue, 30 Jun 2020 00:02:32: 22000000 INFO @ Tue, 30 Jun 2020 00:02:36: 31000000 INFO @ Tue, 30 Jun 2020 00:02:36: 19000000 INFO @ Tue, 30 Jun 2020 00:02:38: 23000000 INFO @ Tue, 30 Jun 2020 00:02:42: 32000000 INFO @ Tue, 30 Jun 2020 00:02:42: 20000000 INFO @ Tue, 30 Jun 2020 00:02:45: 24000000 INFO @ Tue, 30 Jun 2020 00:02:45: #1 tag size is determined as 51 bps INFO @ Tue, 30 Jun 2020 00:02:45: #1 tag size = 51 INFO @ Tue, 30 Jun 2020 00:02:45: #1 total tags in treatment: 32491755 INFO @ Tue, 30 Jun 2020 00:02:45: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:02:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:02:46: #1 tags after filtering in treatment: 32491755 INFO @ Tue, 30 Jun 2020 00:02:46: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:02:46: #1 finished! INFO @ Tue, 30 Jun 2020 00:02:46: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:02:46: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:02:48: #2 number of paired peaks: 73 WARNING @ Tue, 30 Jun 2020 00:02:48: Too few paired peaks (73) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:02:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981609/SRX3981609.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis INFO @ Tue, 30 Jun 2020 00:02:48: 21000000 needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981609/SRX3981609.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981609/SRX3981609.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981609/SRX3981609.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:02:51: 25000000 INFO @ Tue, 30 Jun 2020 00:02:53: 22000000 INFO @ Tue, 30 Jun 2020 00:02:57: 26000000 INFO @ Tue, 30 Jun 2020 00:02:59: 23000000 INFO @ Tue, 30 Jun 2020 00:03:03: 27000000 INFO @ Tue, 30 Jun 2020 00:03:05: 24000000 INFO @ Tue, 30 Jun 2020 00:03:09: 28000000 INFO @ Tue, 30 Jun 2020 00:03:11: 25000000 INFO @ Tue, 30 Jun 2020 00:03:15: 29000000 INFO @ Tue, 30 Jun 2020 00:03:17: 26000000 INFO @ Tue, 30 Jun 2020 00:03:21: 30000000 INFO @ Tue, 30 Jun 2020 00:03:23: 27000000 INFO @ Tue, 30 Jun 2020 00:03:27: 31000000 INFO @ Tue, 30 Jun 2020 00:03:29: 28000000 INFO @ Tue, 30 Jun 2020 00:03:32: 32000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 00:03:34: 29000000 INFO @ Tue, 30 Jun 2020 00:03:36: #1 tag size is determined as 51 bps INFO @ Tue, 30 Jun 2020 00:03:36: #1 tag size = 51 INFO @ Tue, 30 Jun 2020 00:03:36: #1 total tags in treatment: 32491755 INFO @ Tue, 30 Jun 2020 00:03:36: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:03:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:03:36: #1 tags after filtering in treatment: 32491755 INFO @ Tue, 30 Jun 2020 00:03:36: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:03:36: #1 finished! INFO @ Tue, 30 Jun 2020 00:03:36: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:03:36: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:03:38: #2 number of paired peaks: 73 WARNING @ Tue, 30 Jun 2020 00:03:38: Too few paired peaks (73) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:03:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981609/SRX3981609.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981609/SRX3981609.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981609/SRX3981609.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981609/SRX3981609.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:03:40: 30000000 INFO @ Tue, 30 Jun 2020 00:03:45: 31000000 INFO @ Tue, 30 Jun 2020 00:03:51: 32000000 INFO @ Tue, 30 Jun 2020 00:03:54: #1 tag size is determined as 51 bps INFO @ Tue, 30 Jun 2020 00:03:54: #1 tag size = 51 INFO @ Tue, 30 Jun 2020 00:03:54: #1 total tags in treatment: 32491755 INFO @ Tue, 30 Jun 2020 00:03:54: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:03:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:03:55: #1 tags after filtering in treatment: 32491755 INFO @ Tue, 30 Jun 2020 00:03:55: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:03:55: #1 finished! INFO @ Tue, 30 Jun 2020 00:03:55: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:03:55: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:03:57: #2 number of paired peaks: 73 WARNING @ Tue, 30 Jun 2020 00:03:57: Too few paired peaks (73) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:03:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981609/SRX3981609.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981609/SRX3981609.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981609/SRX3981609.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981609/SRX3981609.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。