Job ID = 1295680 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2019-06-03T06:12:39 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) spots read : 57,694,647 reads read : 115,389,294 reads written : 115,389,294 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:06:36 57694647 reads; of these: 57694647 (100.00%) were paired; of these: 9041534 (15.67%) aligned concordantly 0 times 37380803 (64.79%) aligned concordantly exactly 1 time 11272310 (19.54%) aligned concordantly >1 times ---- 9041534 pairs aligned concordantly 0 times; of these: 390692 (4.32%) aligned discordantly 1 time ---- 8650842 pairs aligned 0 times concordantly or discordantly; of these: 17301684 mates make up the pairs; of these: 13222379 (76.42%) aligned 0 times 2861192 (16.54%) aligned exactly 1 time 1218113 (7.04%) aligned >1 times 88.54% overall alignment rate Time searching: 01:06:37 Overall time: 01:06:37 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 28 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 6457658 / 48722705 = 0.1325 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 16:50:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX397054/SRX397054.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX397054/SRX397054.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX397054/SRX397054.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX397054/SRX397054.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 16:50:00: #1 read tag files... INFO @ Mon, 03 Jun 2019 16:50:00: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 16:50:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX397054/SRX397054.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX397054/SRX397054.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX397054/SRX397054.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX397054/SRX397054.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 16:50:00: #1 read tag files... INFO @ Mon, 03 Jun 2019 16:50:00: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 16:50:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX397054/SRX397054.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX397054/SRX397054.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX397054/SRX397054.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX397054/SRX397054.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 16:50:00: #1 read tag files... INFO @ Mon, 03 Jun 2019 16:50:00: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 16:50:05: 1000000 INFO @ Mon, 03 Jun 2019 16:50:05: 1000000 INFO @ Mon, 03 Jun 2019 16:50:05: 1000000 INFO @ Mon, 03 Jun 2019 16:50:10: 2000000 INFO @ Mon, 03 Jun 2019 16:50:10: 2000000 INFO @ Mon, 03 Jun 2019 16:50:10: 2000000 INFO @ Mon, 03 Jun 2019 16:50:15: 3000000 INFO @ Mon, 03 Jun 2019 16:50:15: 3000000 INFO @ Mon, 03 Jun 2019 16:50:15: 3000000 INFO @ Mon, 03 Jun 2019 16:50:19: 4000000 INFO @ Mon, 03 Jun 2019 16:50:19: 4000000 INFO @ Mon, 03 Jun 2019 16:50:19: 4000000 INFO @ Mon, 03 Jun 2019 16:50:24: 5000000 INFO @ Mon, 03 Jun 2019 16:50:24: 5000000 INFO @ Mon, 03 Jun 2019 16:50:24: 5000000 INFO @ Mon, 03 Jun 2019 16:50:29: 6000000 INFO @ Mon, 03 Jun 2019 16:50:29: 6000000 INFO @ Mon, 03 Jun 2019 16:50:29: 6000000 INFO @ Mon, 03 Jun 2019 16:50:33: 7000000 INFO @ Mon, 03 Jun 2019 16:50:33: 7000000 INFO @ Mon, 03 Jun 2019 16:50:33: 7000000 INFO @ Mon, 03 Jun 2019 16:50:38: 8000000 INFO @ Mon, 03 Jun 2019 16:50:38: 8000000 INFO @ Mon, 03 Jun 2019 16:50:38: 8000000 INFO @ Mon, 03 Jun 2019 16:50:43: 9000000 INFO @ Mon, 03 Jun 2019 16:50:43: 9000000 INFO @ Mon, 03 Jun 2019 16:50:43: 9000000 INFO @ Mon, 03 Jun 2019 16:50:47: 10000000 INFO @ Mon, 03 Jun 2019 16:50:47: 10000000 INFO @ Mon, 03 Jun 2019 16:50:47: 10000000 INFO @ Mon, 03 Jun 2019 16:50:52: 11000000 INFO @ Mon, 03 Jun 2019 16:50:52: 11000000 INFO @ Mon, 03 Jun 2019 16:50:52: 11000000 INFO @ Mon, 03 Jun 2019 16:50:56: 12000000 INFO @ Mon, 03 Jun 2019 16:50:56: 12000000 INFO @ Mon, 03 Jun 2019 16:50:56: 12000000 INFO @ Mon, 03 Jun 2019 16:51:01: 13000000 INFO @ Mon, 03 Jun 2019 16:51:01: 13000000 INFO @ Mon, 03 Jun 2019 16:51:01: 13000000 INFO @ Mon, 03 Jun 2019 16:51:06: 14000000 INFO @ Mon, 03 Jun 2019 16:51:06: 14000000 INFO @ Mon, 03 Jun 2019 16:51:06: 14000000 INFO @ Mon, 03 Jun 2019 16:51:10: 15000000 INFO @ Mon, 03 Jun 2019 16:51:10: 15000000 INFO @ Mon, 03 Jun 2019 16:51:10: 15000000 INFO @ Mon, 03 Jun 2019 16:51:15: 16000000 INFO @ Mon, 03 Jun 2019 16:51:15: 16000000 INFO @ Mon, 03 Jun 2019 16:51:15: 16000000 INFO @ Mon, 03 Jun 2019 16:51:19: 17000000 INFO @ Mon, 03 Jun 2019 16:51:19: 17000000 INFO @ Mon, 03 Jun 2019 16:51:20: 17000000 INFO @ Mon, 03 Jun 2019 16:51:24: 18000000 INFO @ Mon, 03 Jun 2019 16:51:24: 18000000 INFO @ Mon, 03 Jun 2019 16:51:25: 18000000 INFO @ Mon, 03 Jun 2019 16:51:29: 19000000 INFO @ Mon, 03 Jun 2019 16:51:29: 19000000 INFO @ Mon, 03 Jun 2019 16:51:29: 19000000 INFO @ Mon, 03 Jun 2019 16:51:33: 20000000 INFO @ Mon, 03 Jun 2019 16:51:33: 20000000 INFO @ Mon, 03 Jun 2019 16:51:34: 20000000 INFO @ Mon, 03 Jun 2019 16:51:38: 21000000 INFO @ Mon, 03 Jun 2019 16:51:38: 21000000 INFO @ Mon, 03 Jun 2019 16:51:39: 21000000 INFO @ Mon, 03 Jun 2019 16:51:43: 22000000 INFO @ Mon, 03 Jun 2019 16:51:43: 22000000 INFO @ Mon, 03 Jun 2019 16:51:44: 22000000 INFO @ Mon, 03 Jun 2019 16:51:47: 23000000 INFO @ Mon, 03 Jun 2019 16:51:47: 23000000 INFO @ Mon, 03 Jun 2019 16:51:49: 23000000 INFO @ Mon, 03 Jun 2019 16:51:52: 24000000 INFO @ Mon, 03 Jun 2019 16:51:52: 24000000 INFO @ Mon, 03 Jun 2019 16:51:54: 24000000 INFO @ Mon, 03 Jun 2019 16:51:57: 25000000 INFO @ Mon, 03 Jun 2019 16:51:57: 25000000 INFO @ Mon, 03 Jun 2019 16:51:59: 25000000 INFO @ Mon, 03 Jun 2019 16:52:02: 26000000 INFO @ Mon, 03 Jun 2019 16:52:02: 26000000 INFO @ Mon, 03 Jun 2019 16:52:05: 26000000 INFO @ Mon, 03 Jun 2019 16:52:07: 27000000 INFO @ Mon, 03 Jun 2019 16:52:07: 27000000 INFO @ Mon, 03 Jun 2019 16:52:10: 27000000 INFO @ Mon, 03 Jun 2019 16:52:12: 28000000 INFO @ Mon, 03 Jun 2019 16:52:12: 28000000 INFO @ Mon, 03 Jun 2019 16:52:15: 28000000 INFO @ Mon, 03 Jun 2019 16:52:17: 29000000 INFO @ Mon, 03 Jun 2019 16:52:17: 29000000 INFO @ Mon, 03 Jun 2019 16:52:21: 29000000 INFO @ Mon, 03 Jun 2019 16:52:22: 30000000 INFO @ Mon, 03 Jun 2019 16:52:23: 30000000 INFO @ Mon, 03 Jun 2019 16:52:26: 30000000 INFO @ Mon, 03 Jun 2019 16:52:28: 31000000 INFO @ Mon, 03 Jun 2019 16:52:28: 31000000 INFO @ Mon, 03 Jun 2019 16:52:31: 31000000 INFO @ Mon, 03 Jun 2019 16:52:32: 32000000 INFO @ Mon, 03 Jun 2019 16:52:32: 32000000 INFO @ Mon, 03 Jun 2019 16:52:36: 32000000 INFO @ Mon, 03 Jun 2019 16:52:37: 33000000 INFO @ Mon, 03 Jun 2019 16:52:37: 33000000 INFO @ Mon, 03 Jun 2019 16:52:41: 33000000 INFO @ Mon, 03 Jun 2019 16:52:42: 34000000 INFO @ Mon, 03 Jun 2019 16:52:42: 34000000 INFO @ Mon, 03 Jun 2019 16:52:46: 34000000 INFO @ Mon, 03 Jun 2019 16:52:46: 35000000 INFO @ Mon, 03 Jun 2019 16:52:47: 35000000 INFO @ Mon, 03 Jun 2019 16:52:51: 35000000 INFO @ Mon, 03 Jun 2019 16:52:51: 36000000 INFO @ Mon, 03 Jun 2019 16:52:51: 36000000 INFO @ Mon, 03 Jun 2019 16:52:56: 36000000 INFO @ Mon, 03 Jun 2019 16:52:56: 37000000 INFO @ Mon, 03 Jun 2019 16:52:56: 37000000 INFO @ Mon, 03 Jun 2019 16:53:01: 38000000 INFO @ Mon, 03 Jun 2019 16:53:01: 38000000 INFO @ Mon, 03 Jun 2019 16:53:01: 37000000 INFO @ Mon, 03 Jun 2019 16:53:05: 39000000 INFO @ Mon, 03 Jun 2019 16:53:05: 39000000 INFO @ Mon, 03 Jun 2019 16:53:06: 38000000 INFO @ Mon, 03 Jun 2019 16:53:10: 40000000 INFO @ Mon, 03 Jun 2019 16:53:10: 40000000 INFO @ Mon, 03 Jun 2019 16:53:11: 39000000 INFO @ Mon, 03 Jun 2019 16:53:15: 41000000 INFO @ Mon, 03 Jun 2019 16:53:15: 41000000 INFO @ Mon, 03 Jun 2019 16:53:15: 40000000 INFO @ Mon, 03 Jun 2019 16:53:19: 42000000 INFO @ Mon, 03 Jun 2019 16:53:19: 42000000 INFO @ Mon, 03 Jun 2019 16:53:20: 41000000 INFO @ Mon, 03 Jun 2019 16:53:24: 43000000 INFO @ Mon, 03 Jun 2019 16:53:24: 43000000 INFO @ Mon, 03 Jun 2019 16:53:25: 42000000 INFO @ Mon, 03 Jun 2019 16:53:29: 44000000 INFO @ Mon, 03 Jun 2019 16:53:29: 44000000 INFO @ Mon, 03 Jun 2019 16:53:30: 43000000 INFO @ Mon, 03 Jun 2019 16:53:33: 45000000 INFO @ Mon, 03 Jun 2019 16:53:33: 45000000 INFO @ Mon, 03 Jun 2019 16:53:35: 44000000 INFO @ Mon, 03 Jun 2019 16:53:38: 46000000 INFO @ Mon, 03 Jun 2019 16:53:38: 46000000 INFO @ Mon, 03 Jun 2019 16:53:39: 45000000 INFO @ Mon, 03 Jun 2019 16:53:43: 47000000 INFO @ Mon, 03 Jun 2019 16:53:43: 47000000 INFO @ Mon, 03 Jun 2019 16:53:44: 46000000 INFO @ Mon, 03 Jun 2019 16:53:47: 48000000 INFO @ Mon, 03 Jun 2019 16:53:47: 48000000 INFO @ Mon, 03 Jun 2019 16:53:49: 47000000 INFO @ Mon, 03 Jun 2019 16:53:52: 49000000 INFO @ Mon, 03 Jun 2019 16:53:52: 49000000 INFO @ Mon, 03 Jun 2019 16:53:54: 48000000 INFO @ Mon, 03 Jun 2019 16:53:57: 50000000 INFO @ Mon, 03 Jun 2019 16:53:57: 50000000 INFO @ Mon, 03 Jun 2019 16:53:59: 49000000 INFO @ Mon, 03 Jun 2019 16:54:01: 51000000 INFO @ Mon, 03 Jun 2019 16:54:01: 51000000 INFO @ Mon, 03 Jun 2019 16:54:04: 50000000 INFO @ Mon, 03 Jun 2019 16:54:06: 52000000 INFO @ Mon, 03 Jun 2019 16:54:06: 52000000 INFO @ Mon, 03 Jun 2019 16:54:09: 51000000 INFO @ Mon, 03 Jun 2019 16:54:11: 53000000 INFO @ Mon, 03 Jun 2019 16:54:11: 53000000 INFO @ Mon, 03 Jun 2019 16:54:13: 52000000 INFO @ Mon, 03 Jun 2019 16:54:15: 54000000 INFO @ Mon, 03 Jun 2019 16:54:15: 54000000 INFO @ Mon, 03 Jun 2019 16:54:18: 53000000 INFO @ Mon, 03 Jun 2019 16:54:20: 55000000 INFO @ Mon, 03 Jun 2019 16:54:20: 55000000 INFO @ Mon, 03 Jun 2019 16:54:23: 54000000 INFO @ Mon, 03 Jun 2019 16:54:25: 56000000 INFO @ Mon, 03 Jun 2019 16:54:25: 56000000 INFO @ Mon, 03 Jun 2019 16:54:28: 55000000 INFO @ Mon, 03 Jun 2019 16:54:30: 57000000 INFO @ Mon, 03 Jun 2019 16:54:30: 57000000 INFO @ Mon, 03 Jun 2019 16:54:34: 56000000 INFO @ Mon, 03 Jun 2019 16:54:35: 58000000 INFO @ Mon, 03 Jun 2019 16:54:35: 58000000 INFO @ Mon, 03 Jun 2019 16:54:39: 57000000 INFO @ Mon, 03 Jun 2019 16:54:40: 59000000 INFO @ Mon, 03 Jun 2019 16:54:40: 59000000 INFO @ Mon, 03 Jun 2019 16:54:44: 58000000 INFO @ Mon, 03 Jun 2019 16:54:45: 60000000 INFO @ Mon, 03 Jun 2019 16:54:45: 60000000 INFO @ Mon, 03 Jun 2019 16:54:50: 59000000 INFO @ Mon, 03 Jun 2019 16:54:50: 61000000 INFO @ Mon, 03 Jun 2019 16:54:50: 61000000 INFO @ Mon, 03 Jun 2019 16:54:55: 60000000 INFO @ Mon, 03 Jun 2019 16:54:55: 62000000 INFO @ Mon, 03 Jun 2019 16:54:55: 62000000 INFO @ Mon, 03 Jun 2019 16:55:00: 63000000 INFO @ Mon, 03 Jun 2019 16:55:00: 63000000 INFO @ Mon, 03 Jun 2019 16:55:00: 61000000 INFO @ Mon, 03 Jun 2019 16:55:04: 64000000 INFO @ Mon, 03 Jun 2019 16:55:04: 64000000 INFO @ Mon, 03 Jun 2019 16:55:05: 62000000 INFO @ Mon, 03 Jun 2019 16:55:09: 65000000 INFO @ Mon, 03 Jun 2019 16:55:09: 65000000 INFO @ Mon, 03 Jun 2019 16:55:09: 63000000 INFO @ Mon, 03 Jun 2019 16:55:14: 66000000 INFO @ Mon, 03 Jun 2019 16:55:14: 66000000 INFO @ Mon, 03 Jun 2019 16:55:14: 64000000 INFO @ Mon, 03 Jun 2019 16:55:18: 67000000 INFO @ Mon, 03 Jun 2019 16:55:18: 67000000 INFO @ Mon, 03 Jun 2019 16:55:19: 65000000 INFO @ Mon, 03 Jun 2019 16:55:23: 68000000 INFO @ Mon, 03 Jun 2019 16:55:23: 68000000 INFO @ Mon, 03 Jun 2019 16:55:24: 66000000 INFO @ Mon, 03 Jun 2019 16:55:28: 69000000 INFO @ Mon, 03 Jun 2019 16:55:28: 69000000 INFO @ Mon, 03 Jun 2019 16:55:29: 67000000 INFO @ Mon, 03 Jun 2019 16:55:32: 70000000 INFO @ Mon, 03 Jun 2019 16:55:32: 70000000 INFO @ Mon, 03 Jun 2019 16:55:34: 68000000 INFO @ Mon, 03 Jun 2019 16:55:37: 71000000 INFO @ Mon, 03 Jun 2019 16:55:37: 71000000 INFO @ Mon, 03 Jun 2019 16:55:39: 69000000 INFO @ Mon, 03 Jun 2019 16:55:42: 72000000 INFO @ Mon, 03 Jun 2019 16:55:42: 72000000 INFO @ Mon, 03 Jun 2019 16:55:44: 70000000 INFO @ Mon, 03 Jun 2019 16:55:47: 73000000 INFO @ Mon, 03 Jun 2019 16:55:47: 73000000 INFO @ Mon, 03 Jun 2019 16:55:48: 71000000 INFO @ Mon, 03 Jun 2019 16:55:51: 74000000 INFO @ Mon, 03 Jun 2019 16:55:51: 74000000 INFO @ Mon, 03 Jun 2019 16:55:53: 72000000 INFO @ Mon, 03 Jun 2019 16:55:56: 75000000 INFO @ Mon, 03 Jun 2019 16:55:56: 75000000 INFO @ Mon, 03 Jun 2019 16:55:58: 73000000 INFO @ Mon, 03 Jun 2019 16:56:01: 76000000 INFO @ Mon, 03 Jun 2019 16:56:01: 76000000 INFO @ Mon, 03 Jun 2019 16:56:03: 74000000 INFO @ Mon, 03 Jun 2019 16:56:05: 77000000 INFO @ Mon, 03 Jun 2019 16:56:05: 77000000 INFO @ Mon, 03 Jun 2019 16:56:08: 75000000 INFO @ Mon, 03 Jun 2019 16:56:10: 78000000 INFO @ Mon, 03 Jun 2019 16:56:10: 78000000 INFO @ Mon, 03 Jun 2019 16:56:13: 76000000 INFO @ Mon, 03 Jun 2019 16:56:15: 79000000 INFO @ Mon, 03 Jun 2019 16:56:15: 79000000 INFO @ Mon, 03 Jun 2019 16:56:18: 77000000 INFO @ Mon, 03 Jun 2019 16:56:19: 80000000 INFO @ Mon, 03 Jun 2019 16:56:19: 80000000 INFO @ Mon, 03 Jun 2019 16:56:23: 78000000 INFO @ Mon, 03 Jun 2019 16:56:24: 81000000 INFO @ Mon, 03 Jun 2019 16:56:24: 81000000 INFO @ Mon, 03 Jun 2019 16:56:28: 79000000 INFO @ Mon, 03 Jun 2019 16:56:29: 82000000 INFO @ Mon, 03 Jun 2019 16:56:29: 82000000 INFO @ Mon, 03 Jun 2019 16:56:32: 80000000 INFO @ Mon, 03 Jun 2019 16:56:34: 83000000 INFO @ Mon, 03 Jun 2019 16:56:34: 83000000 INFO @ Mon, 03 Jun 2019 16:56:38: 81000000 INFO @ Mon, 03 Jun 2019 16:56:39: 84000000 INFO @ Mon, 03 Jun 2019 16:56:39: 84000000 INFO @ Mon, 03 Jun 2019 16:56:43: 82000000 INFO @ Mon, 03 Jun 2019 16:56:43: 85000000 INFO @ Mon, 03 Jun 2019 16:56:43: 85000000 INFO @ Mon, 03 Jun 2019 16:56:48: 83000000 INFO @ Mon, 03 Jun 2019 16:56:48: 86000000 INFO @ Mon, 03 Jun 2019 16:56:48: 86000000 INFO @ Mon, 03 Jun 2019 16:56:53: 84000000 INFO @ Mon, 03 Jun 2019 16:56:53: 87000000 INFO @ Mon, 03 Jun 2019 16:56:53: 87000000 INFO @ Mon, 03 Jun 2019 16:56:58: 88000000 INFO @ Mon, 03 Jun 2019 16:56:58: 88000000 INFO @ Mon, 03 Jun 2019 16:56:58: 85000000 INFO @ Mon, 03 Jun 2019 16:57:03: 89000000 INFO @ Mon, 03 Jun 2019 16:57:03: 89000000 INFO @ Mon, 03 Jun 2019 16:57:03: 86000000 INFO @ Mon, 03 Jun 2019 16:57:05: #1 tag size is determined as 24 bps INFO @ Mon, 03 Jun 2019 16:57:05: #1 tag size = 24 INFO @ Mon, 03 Jun 2019 16:57:05: #1 total tags in treatment: 42197583 INFO @ Mon, 03 Jun 2019 16:57:05: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 16:57:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 16:57:05: #1 tag size is determined as 24 bps INFO @ Mon, 03 Jun 2019 16:57:05: #1 tag size = 24 INFO @ Mon, 03 Jun 2019 16:57:05: #1 total tags in treatment: 42197583 INFO @ Mon, 03 Jun 2019 16:57:05: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 16:57:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 16:57:06: #1 tags after filtering in treatment: 27481048 INFO @ Mon, 03 Jun 2019 16:57:06: #1 Redundant rate of treatment: 0.35 INFO @ Mon, 03 Jun 2019 16:57:06: #1 finished! INFO @ Mon, 03 Jun 2019 16:57:06: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 16:57:06: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 16:57:06: #1 tags after filtering in treatment: 27481048 INFO @ Mon, 03 Jun 2019 16:57:06: #1 Redundant rate of treatment: 0.35 INFO @ Mon, 03 Jun 2019 16:57:06: #1 finished! INFO @ Mon, 03 Jun 2019 16:57:06: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 16:57:06: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 16:57:07: #2 number of paired peaks: 1 WARNING @ Mon, 03 Jun 2019 16:57:07: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 16:57:07: Process for pairing-model is terminated! INFO @ Mon, 03 Jun 2019 16:57:07: #2 number of paired peaks: 1 WARNING @ Mon, 03 Jun 2019 16:57:07: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 16:57:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX397054/SRX397054.10_peaks.narrowPeak: No such file or directory cut: /home/okishinya/chipatlas/results/dm3/SRX397054/SRX397054.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms)pass1 - making usageList (0 chroms): 1 millis : 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX397054/SRX397054.05_model.r’: No such file or directoryrm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX397054/SRX397054.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX397054/SRX397054.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX397054/SRX397054.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX397054/SRX397054.05_peaks.narrowPeak’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX397054/SRX397054.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 16:57:08: 87000000 INFO @ Mon, 03 Jun 2019 16:57:12: 88000000 INFO @ Mon, 03 Jun 2019 16:57:17: 89000000 INFO @ Mon, 03 Jun 2019 16:57:18: #1 tag size is determined as 24 bps INFO @ Mon, 03 Jun 2019 16:57:18: #1 tag size = 24 INFO @ Mon, 03 Jun 2019 16:57:18: #1 total tags in treatment: 42197583 INFO @ Mon, 03 Jun 2019 16:57:18: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 16:57:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 16:57:19: #1 tags after filtering in treatment: 27481048 INFO @ Mon, 03 Jun 2019 16:57:19: #1 Redundant rate of treatment: 0.35 INFO @ Mon, 03 Jun 2019 16:57:19: #1 finished! INFO @ Mon, 03 Jun 2019 16:57:19: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 16:57:19: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 16:57:21: #2 number of paired peaks: 1 WARNING @ Mon, 03 Jun 2019 16:57:21: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 16:57:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX397054/SRX397054.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX397054/SRX397054.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX397054/SRX397054.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX397054/SRX397054.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。