Job ID = 10845229 sra ファイルのダウンロード中... Completed: 178235K bytes transferred in 4 seconds (294911K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 3700347 spots for /home/okishinya/chipatlas/results/dm3/SRX3937191/SRR7004611.sra Written 3700347 spots for /home/okishinya/chipatlas/results/dm3/SRX3937191/SRR7004611.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:01 3700347 reads; of these: 3700347 (100.00%) were unpaired; of these: 992454 (26.82%) aligned 0 times 2036634 (55.04%) aligned exactly 1 time 671259 (18.14%) aligned >1 times 73.18% overall alignment rate Time searching: 00:02:01 Overall time: 00:02:02 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 192342 / 2707893 = 0.0710 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 04 Jul 2018 09:33:32: # Command line: callpeak -t SRX3937191.bam -f BAM -g dm -n SRX3937191.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX3937191.05 # format = BAM # ChIP-seq file = ['SRX3937191.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 04 Jul 2018 09:33:32: #1 read tag files... INFO @ Wed, 04 Jul 2018 09:33:32: #1 read treatment tags... INFO @ Wed, 04 Jul 2018 09:33:32: # Command line: callpeak -t SRX3937191.bam -f BAM -g dm -n SRX3937191.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX3937191.10 # format = BAM # ChIP-seq file = ['SRX3937191.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 04 Jul 2018 09:33:32: #1 read tag files... INFO @ Wed, 04 Jul 2018 09:33:32: #1 read treatment tags... INFO @ Wed, 04 Jul 2018 09:33:32: # Command line: callpeak -t SRX3937191.bam -f BAM -g dm -n SRX3937191.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX3937191.20 # format = BAM # ChIP-seq file = ['SRX3937191.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 04 Jul 2018 09:33:32: #1 read tag files... INFO @ Wed, 04 Jul 2018 09:33:32: #1 read treatment tags... INFO @ Wed, 04 Jul 2018 09:33:42: 1000000 INFO @ Wed, 04 Jul 2018 09:33:42: 1000000 INFO @ Wed, 04 Jul 2018 09:33:42: 1000000 INFO @ Wed, 04 Jul 2018 09:33:51: 2000000 INFO @ Wed, 04 Jul 2018 09:33:51: 2000000 INFO @ Wed, 04 Jul 2018 09:33:51: 2000000 INFO @ Wed, 04 Jul 2018 09:33:56: #1 tag size is determined as 99 bps INFO @ Wed, 04 Jul 2018 09:33:56: #1 tag size = 99 INFO @ Wed, 04 Jul 2018 09:33:56: #1 total tags in treatment: 2515551 INFO @ Wed, 04 Jul 2018 09:33:56: #1 user defined the maximum tags... INFO @ Wed, 04 Jul 2018 09:33:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 04 Jul 2018 09:33:56: #1 tags after filtering in treatment: 2515551 INFO @ Wed, 04 Jul 2018 09:33:56: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 04 Jul 2018 09:33:56: #1 finished! INFO @ Wed, 04 Jul 2018 09:33:56: #2 Build Peak Model... INFO @ Wed, 04 Jul 2018 09:33:56: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 04 Jul 2018 09:33:56: #2 number of paired peaks: 1367 INFO @ Wed, 04 Jul 2018 09:33:56: start model_add_line... INFO @ Wed, 04 Jul 2018 09:33:56: start X-correlation... INFO @ Wed, 04 Jul 2018 09:33:56: end of X-cor INFO @ Wed, 04 Jul 2018 09:33:56: #2 finished! INFO @ Wed, 04 Jul 2018 09:33:56: #2 predicted fragment length is 109 bps INFO @ Wed, 04 Jul 2018 09:33:56: #2 alternative fragment length(s) may be 109 bps INFO @ Wed, 04 Jul 2018 09:33:56: #2.2 Generate R script for model : SRX3937191.20_model.r WARNING @ Wed, 04 Jul 2018 09:33:56: #2 Since the d (109) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Wed, 04 Jul 2018 09:33:56: #2 You may need to consider one of the other alternative d(s): 109 WARNING @ Wed, 04 Jul 2018 09:33:56: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Wed, 04 Jul 2018 09:33:56: #3 Call peaks... INFO @ Wed, 04 Jul 2018 09:33:56: #3 Pre-compute pvalue-qvalue table... INFO @ Wed, 04 Jul 2018 09:33:56: #1 tag size is determined as 99 bps INFO @ Wed, 04 Jul 2018 09:33:56: #1 tag size = 99 INFO @ Wed, 04 Jul 2018 09:33:56: #1 total tags in treatment: 2515551 INFO @ Wed, 04 Jul 2018 09:33:56: #1 user defined the maximum tags... INFO @ Wed, 04 Jul 2018 09:33:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 04 Jul 2018 09:33:56: #1 tags after filtering in treatment: 2515551 INFO @ Wed, 04 Jul 2018 09:33:56: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 04 Jul 2018 09:33:56: #1 finished! INFO @ Wed, 04 Jul 2018 09:33:56: #2 Build Peak Model... INFO @ Wed, 04 Jul 2018 09:33:56: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 04 Jul 2018 09:33:56: #1 tag size is determined as 99 bps INFO @ Wed, 04 Jul 2018 09:33:56: #1 tag size = 99 INFO @ Wed, 04 Jul 2018 09:33:56: #1 total tags in treatment: 2515551 INFO @ Wed, 04 Jul 2018 09:33:56: #1 user defined the maximum tags... INFO @ Wed, 04 Jul 2018 09:33:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 04 Jul 2018 09:33:56: #1 tags after filtering in treatment: 2515551 INFO @ Wed, 04 Jul 2018 09:33:56: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 04 Jul 2018 09:33:56: #1 finished! INFO @ Wed, 04 Jul 2018 09:33:56: #2 Build Peak Model... INFO @ Wed, 04 Jul 2018 09:33:56: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 04 Jul 2018 09:33:56: #2 number of paired peaks: 1367 INFO @ Wed, 04 Jul 2018 09:33:56: start model_add_line... INFO @ Wed, 04 Jul 2018 09:33:56: start X-correlation... INFO @ Wed, 04 Jul 2018 09:33:56: end of X-cor INFO @ Wed, 04 Jul 2018 09:33:56: #2 finished! INFO @ Wed, 04 Jul 2018 09:33:56: #2 predicted fragment length is 109 bps INFO @ Wed, 04 Jul 2018 09:33:56: #2 alternative fragment length(s) may be 109 bps INFO @ Wed, 04 Jul 2018 09:33:56: #2.2 Generate R script for model : SRX3937191.05_model.r WARNING @ Wed, 04 Jul 2018 09:33:56: #2 Since the d (109) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Wed, 04 Jul 2018 09:33:56: #2 You may need to consider one of the other alternative d(s): 109 WARNING @ Wed, 04 Jul 2018 09:33:56: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Wed, 04 Jul 2018 09:33:56: #3 Call peaks... INFO @ Wed, 04 Jul 2018 09:33:56: #3 Pre-compute pvalue-qvalue table... INFO @ Wed, 04 Jul 2018 09:33:57: #2 number of paired peaks: 1367 INFO @ Wed, 04 Jul 2018 09:33:57: start model_add_line... INFO @ Wed, 04 Jul 2018 09:33:57: start X-correlation... INFO @ Wed, 04 Jul 2018 09:33:57: end of X-cor INFO @ Wed, 04 Jul 2018 09:33:57: #2 finished! INFO @ Wed, 04 Jul 2018 09:33:57: #2 predicted fragment length is 109 bps INFO @ Wed, 04 Jul 2018 09:33:57: #2 alternative fragment length(s) may be 109 bps INFO @ Wed, 04 Jul 2018 09:33:57: #2.2 Generate R script for model : SRX3937191.10_model.r WARNING @ Wed, 04 Jul 2018 09:33:57: #2 Since the d (109) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Wed, 04 Jul 2018 09:33:57: #2 You may need to consider one of the other alternative d(s): 109 WARNING @ Wed, 04 Jul 2018 09:33:57: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Wed, 04 Jul 2018 09:33:57: #3 Call peaks... INFO @ Wed, 04 Jul 2018 09:33:57: #3 Pre-compute pvalue-qvalue table... INFO @ Wed, 04 Jul 2018 09:34:02: #3 Call peaks for each chromosome... INFO @ Wed, 04 Jul 2018 09:34:02: #3 Call peaks for each chromosome... INFO @ Wed, 04 Jul 2018 09:34:03: #3 Call peaks for each chromosome... INFO @ Wed, 04 Jul 2018 09:34:05: #4 Write output xls file... SRX3937191.20_peaks.xls INFO @ Wed, 04 Jul 2018 09:34:05: #4 Write peak in narrowPeak format file... SRX3937191.20_peaks.narrowPeak INFO @ Wed, 04 Jul 2018 09:34:05: #4 Write summits bed file... SRX3937191.20_summits.bed INFO @ Wed, 04 Jul 2018 09:34:05: Done! pass1 - making usageList (5 chroms): 1 millis pass2 - checking and writing primary data (216 records, 4 fields): 1 millis CompletedMACS2peakCalling INFO @ Wed, 04 Jul 2018 09:34:05: #4 Write output xls file... SRX3937191.05_peaks.xls INFO @ Wed, 04 Jul 2018 09:34:05: #4 Write peak in narrowPeak format file... SRX3937191.05_peaks.narrowPeak INFO @ Wed, 04 Jul 2018 09:34:05: #4 Write summits bed file... SRX3937191.05_summits.bed INFO @ Wed, 04 Jul 2018 09:34:05: Done! pass1 - making usageList (12 chroms): 1 millis pass2 - checking and writing primary data (659 records, 4 fields): 3 millis CompletedMACS2peakCalling INFO @ Wed, 04 Jul 2018 09:34:06: #4 Write output xls file... SRX3937191.10_peaks.xls INFO @ Wed, 04 Jul 2018 09:34:06: #4 Write peak in narrowPeak format file... SRX3937191.10_peaks.narrowPeak INFO @ Wed, 04 Jul 2018 09:34:06: #4 Write summits bed file... SRX3937191.10_summits.bed INFO @ Wed, 04 Jul 2018 09:34:06: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (420 records, 4 fields): 4 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。