Job ID = 1295617 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 48,045,955 reads read : 48,045,955 reads written : 48,045,955 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:20:57 48045955 reads; of these: 48045955 (100.00%) were unpaired; of these: 2849234 (5.93%) aligned 0 times 33987749 (70.74%) aligned exactly 1 time 11208972 (23.33%) aligned >1 times 94.07% overall alignment rate Time searching: 00:20:57 Overall time: 00:20:57 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 11810752 / 45196721 = 0.2613 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 15:16:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX375354/SRX375354.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX375354/SRX375354.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX375354/SRX375354.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX375354/SRX375354.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 15:16:33: #1 read tag files... INFO @ Mon, 03 Jun 2019 15:16:33: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 15:16:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX375354/SRX375354.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX375354/SRX375354.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX375354/SRX375354.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX375354/SRX375354.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 15:16:33: #1 read tag files... INFO @ Mon, 03 Jun 2019 15:16:33: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 15:16:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX375354/SRX375354.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX375354/SRX375354.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX375354/SRX375354.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX375354/SRX375354.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 15:16:33: #1 read tag files... INFO @ Mon, 03 Jun 2019 15:16:33: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 15:16:42: 1000000 INFO @ Mon, 03 Jun 2019 15:16:42: 1000000 INFO @ Mon, 03 Jun 2019 15:16:44: 1000000 INFO @ Mon, 03 Jun 2019 15:16:51: 2000000 INFO @ Mon, 03 Jun 2019 15:16:52: 2000000 INFO @ Mon, 03 Jun 2019 15:16:52: 2000000 INFO @ Mon, 03 Jun 2019 15:16:59: 3000000 INFO @ Mon, 03 Jun 2019 15:17:01: 3000000 INFO @ Mon, 03 Jun 2019 15:17:01: 3000000 INFO @ Mon, 03 Jun 2019 15:17:08: 4000000 INFO @ Mon, 03 Jun 2019 15:17:10: 4000000 INFO @ Mon, 03 Jun 2019 15:17:10: 4000000 INFO @ Mon, 03 Jun 2019 15:17:16: 5000000 INFO @ Mon, 03 Jun 2019 15:17:20: 5000000 INFO @ Mon, 03 Jun 2019 15:17:20: 5000000 INFO @ Mon, 03 Jun 2019 15:17:24: 6000000 INFO @ Mon, 03 Jun 2019 15:17:28: 6000000 INFO @ Mon, 03 Jun 2019 15:17:30: 6000000 INFO @ Mon, 03 Jun 2019 15:17:31: 7000000 INFO @ Mon, 03 Jun 2019 15:17:37: 7000000 INFO @ Mon, 03 Jun 2019 15:17:39: 8000000 INFO @ Mon, 03 Jun 2019 15:17:40: 7000000 INFO @ Mon, 03 Jun 2019 15:17:46: 9000000 INFO @ Mon, 03 Jun 2019 15:17:46: 8000000 INFO @ Mon, 03 Jun 2019 15:17:50: 8000000 INFO @ Mon, 03 Jun 2019 15:17:54: 10000000 INFO @ Mon, 03 Jun 2019 15:17:56: 9000000 INFO @ Mon, 03 Jun 2019 15:18:00: 9000000 INFO @ Mon, 03 Jun 2019 15:18:02: 11000000 INFO @ Mon, 03 Jun 2019 15:18:06: 10000000 INFO @ Mon, 03 Jun 2019 15:18:09: 12000000 INFO @ Mon, 03 Jun 2019 15:18:10: 10000000 INFO @ Mon, 03 Jun 2019 15:18:16: 11000000 INFO @ Mon, 03 Jun 2019 15:18:17: 13000000 INFO @ Mon, 03 Jun 2019 15:18:20: 11000000 INFO @ Mon, 03 Jun 2019 15:18:24: 14000000 INFO @ Mon, 03 Jun 2019 15:18:25: 12000000 INFO @ Mon, 03 Jun 2019 15:18:29: 12000000 INFO @ Mon, 03 Jun 2019 15:18:32: 15000000 INFO @ Mon, 03 Jun 2019 15:18:35: 13000000 INFO @ Mon, 03 Jun 2019 15:18:39: 13000000 INFO @ Mon, 03 Jun 2019 15:18:40: 16000000 INFO @ Mon, 03 Jun 2019 15:18:45: 14000000 INFO @ Mon, 03 Jun 2019 15:18:48: 17000000 INFO @ Mon, 03 Jun 2019 15:18:49: 14000000 INFO @ Mon, 03 Jun 2019 15:18:54: 15000000 INFO @ Mon, 03 Jun 2019 15:18:56: 18000000 INFO @ Mon, 03 Jun 2019 15:18:58: 15000000 INFO @ Mon, 03 Jun 2019 15:19:03: 19000000 INFO @ Mon, 03 Jun 2019 15:19:04: 16000000 INFO @ Mon, 03 Jun 2019 15:19:08: 16000000 INFO @ Mon, 03 Jun 2019 15:19:11: 20000000 INFO @ Mon, 03 Jun 2019 15:19:13: 17000000 INFO @ Mon, 03 Jun 2019 15:19:18: 17000000 INFO @ Mon, 03 Jun 2019 15:19:18: 21000000 INFO @ Mon, 03 Jun 2019 15:19:23: 18000000 INFO @ Mon, 03 Jun 2019 15:19:26: 22000000 INFO @ Mon, 03 Jun 2019 15:19:28: 18000000 INFO @ Mon, 03 Jun 2019 15:19:33: 19000000 INFO @ Mon, 03 Jun 2019 15:19:34: 23000000 INFO @ Mon, 03 Jun 2019 15:19:38: 19000000 INFO @ Mon, 03 Jun 2019 15:19:42: 24000000 INFO @ Mon, 03 Jun 2019 15:19:42: 20000000 INFO @ Mon, 03 Jun 2019 15:19:48: 20000000 INFO @ Mon, 03 Jun 2019 15:19:50: 25000000 INFO @ Mon, 03 Jun 2019 15:19:52: 21000000 INFO @ Mon, 03 Jun 2019 15:19:58: 26000000 INFO @ Mon, 03 Jun 2019 15:19:59: 21000000 INFO @ Mon, 03 Jun 2019 15:20:01: 22000000 INFO @ Mon, 03 Jun 2019 15:20:06: 27000000 INFO @ Mon, 03 Jun 2019 15:20:08: 22000000 INFO @ Mon, 03 Jun 2019 15:20:11: 23000000 INFO @ Mon, 03 Jun 2019 15:20:13: 28000000 INFO @ Mon, 03 Jun 2019 15:20:18: 23000000 INFO @ Mon, 03 Jun 2019 15:20:21: 24000000 INFO @ Mon, 03 Jun 2019 15:20:21: 29000000 INFO @ Mon, 03 Jun 2019 15:20:28: 24000000 INFO @ Mon, 03 Jun 2019 15:20:28: 30000000 INFO @ Mon, 03 Jun 2019 15:20:30: 25000000 INFO @ Mon, 03 Jun 2019 15:20:36: 31000000 INFO @ Mon, 03 Jun 2019 15:20:38: 25000000 INFO @ Mon, 03 Jun 2019 15:20:40: 26000000 INFO @ Mon, 03 Jun 2019 15:20:45: 32000000 INFO @ Mon, 03 Jun 2019 15:20:48: 26000000 INFO @ Mon, 03 Jun 2019 15:20:49: 27000000 INFO @ Mon, 03 Jun 2019 15:20:53: 33000000 INFO @ Mon, 03 Jun 2019 15:20:56: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 15:20:56: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 15:20:56: #1 total tags in treatment: 33385969 INFO @ Mon, 03 Jun 2019 15:20:56: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 15:20:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 15:20:57: #1 tags after filtering in treatment: 33385969 INFO @ Mon, 03 Jun 2019 15:20:57: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 15:20:57: #1 finished! INFO @ Mon, 03 Jun 2019 15:20:57: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 15:20:57: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 15:20:58: 27000000 INFO @ Mon, 03 Jun 2019 15:21:00: 28000000 INFO @ Mon, 03 Jun 2019 15:21:00: #2 number of paired peaks: 45 WARNING @ Mon, 03 Jun 2019 15:21:00: Too few paired peaks (45) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 15:21:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX375354/SRX375354.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX375354/SRX375354.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX375354/SRX375354.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX375354/SRX375354.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 15:21:08: 28000000 INFO @ Mon, 03 Jun 2019 15:21:10: 29000000 INFO @ Mon, 03 Jun 2019 15:21:16: 29000000 INFO @ Mon, 03 Jun 2019 15:21:19: 30000000 INFO @ Mon, 03 Jun 2019 15:21:25: 30000000 INFO @ Mon, 03 Jun 2019 15:21:28: 31000000 INFO @ Mon, 03 Jun 2019 15:21:34: 31000000 INFO @ Mon, 03 Jun 2019 15:21:37: 32000000 INFO @ Mon, 03 Jun 2019 15:21:43: 32000000 INFO @ Mon, 03 Jun 2019 15:21:46: 33000000 INFO @ Mon, 03 Jun 2019 15:21:49: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 15:21:49: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 15:21:49: #1 total tags in treatment: 33385969 INFO @ Mon, 03 Jun 2019 15:21:49: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 15:21:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 15:21:50: #1 tags after filtering in treatment: 33385969 INFO @ Mon, 03 Jun 2019 15:21:50: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 15:21:50: #1 finished! INFO @ Mon, 03 Jun 2019 15:21:50: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 15:21:50: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 15:21:52: 33000000 INFO @ Mon, 03 Jun 2019 15:21:53: #2 number of paired peaks: 45 WARNING @ Mon, 03 Jun 2019 15:21:53: Too few paired peaks (45) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 15:21:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX375354/SRX375354.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX375354/SRX375354.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX375354/SRX375354.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX375354/SRX375354.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 15:21:56: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 15:21:56: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 15:21:56: #1 total tags in treatment: 33385969 INFO @ Mon, 03 Jun 2019 15:21:56: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 15:21:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 15:21:56: #1 tags after filtering in treatment: 33385969 INFO @ Mon, 03 Jun 2019 15:21:56: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 15:21:56: #1 finished! INFO @ Mon, 03 Jun 2019 15:21:56: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 15:21:56: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 15:21:59: #2 number of paired peaks: 45 WARNING @ Mon, 03 Jun 2019 15:21:59: Too few paired peaks (45) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 15:21:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX375354/SRX375354.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX375354/SRX375354.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX375354/SRX375354.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX375354/SRX375354.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。