Job ID = 1295612 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 38,060,396 reads read : 38,060,396 reads written : 38,060,396 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:15:37 38060396 reads; of these: 38060396 (100.00%) were unpaired; of these: 2959708 (7.78%) aligned 0 times 26035042 (68.40%) aligned exactly 1 time 9065646 (23.82%) aligned >1 times 92.22% overall alignment rate Time searching: 00:15:37 Overall time: 00:15:37 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 7173655 / 35100688 = 0.2044 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 15:05:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX375352/SRX375352.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX375352/SRX375352.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX375352/SRX375352.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX375352/SRX375352.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 15:05:43: #1 read tag files... INFO @ Mon, 03 Jun 2019 15:05:43: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 15:05:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX375352/SRX375352.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX375352/SRX375352.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX375352/SRX375352.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX375352/SRX375352.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 15:05:43: #1 read tag files... INFO @ Mon, 03 Jun 2019 15:05:43: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 15:05:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX375352/SRX375352.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX375352/SRX375352.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX375352/SRX375352.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX375352/SRX375352.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 15:05:43: #1 read tag files... INFO @ Mon, 03 Jun 2019 15:05:43: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 15:05:52: 1000000 INFO @ Mon, 03 Jun 2019 15:05:52: 1000000 INFO @ Mon, 03 Jun 2019 15:05:53: 1000000 INFO @ Mon, 03 Jun 2019 15:06:01: 2000000 INFO @ Mon, 03 Jun 2019 15:06:01: 2000000 INFO @ Mon, 03 Jun 2019 15:06:02: 2000000 INFO @ Mon, 03 Jun 2019 15:06:10: 3000000 INFO @ Mon, 03 Jun 2019 15:06:10: 3000000 INFO @ Mon, 03 Jun 2019 15:06:11: 3000000 INFO @ Mon, 03 Jun 2019 15:06:18: 4000000 INFO @ Mon, 03 Jun 2019 15:06:18: 4000000 INFO @ Mon, 03 Jun 2019 15:06:20: 4000000 INFO @ Mon, 03 Jun 2019 15:06:25: 5000000 INFO @ Mon, 03 Jun 2019 15:06:27: 5000000 INFO @ Mon, 03 Jun 2019 15:06:28: 5000000 INFO @ Mon, 03 Jun 2019 15:06:33: 6000000 INFO @ Mon, 03 Jun 2019 15:06:36: 6000000 INFO @ Mon, 03 Jun 2019 15:06:36: 6000000 INFO @ Mon, 03 Jun 2019 15:06:41: 7000000 INFO @ Mon, 03 Jun 2019 15:06:44: 7000000 INFO @ Mon, 03 Jun 2019 15:06:45: 7000000 INFO @ Mon, 03 Jun 2019 15:06:49: 8000000 INFO @ Mon, 03 Jun 2019 15:06:53: 8000000 INFO @ Mon, 03 Jun 2019 15:06:55: 8000000 INFO @ Mon, 03 Jun 2019 15:06:57: 9000000 INFO @ Mon, 03 Jun 2019 15:07:02: 9000000 INFO @ Mon, 03 Jun 2019 15:07:04: 9000000 INFO @ Mon, 03 Jun 2019 15:07:05: 10000000 INFO @ Mon, 03 Jun 2019 15:07:10: 10000000 INFO @ Mon, 03 Jun 2019 15:07:12: 10000000 INFO @ Mon, 03 Jun 2019 15:07:13: 11000000 INFO @ Mon, 03 Jun 2019 15:07:19: 11000000 INFO @ Mon, 03 Jun 2019 15:07:21: 12000000 INFO @ Mon, 03 Jun 2019 15:07:21: 11000000 INFO @ Mon, 03 Jun 2019 15:07:27: 12000000 INFO @ Mon, 03 Jun 2019 15:07:29: 13000000 INFO @ Mon, 03 Jun 2019 15:07:29: 12000000 INFO @ Mon, 03 Jun 2019 15:07:36: 13000000 INFO @ Mon, 03 Jun 2019 15:07:36: 14000000 INFO @ Mon, 03 Jun 2019 15:07:38: 13000000 INFO @ Mon, 03 Jun 2019 15:07:44: 15000000 INFO @ Mon, 03 Jun 2019 15:07:44: 14000000 INFO @ Mon, 03 Jun 2019 15:07:46: 14000000 INFO @ Mon, 03 Jun 2019 15:07:53: 16000000 INFO @ Mon, 03 Jun 2019 15:07:53: 15000000 INFO @ Mon, 03 Jun 2019 15:07:55: 15000000 INFO @ Mon, 03 Jun 2019 15:08:01: 17000000 INFO @ Mon, 03 Jun 2019 15:08:02: 16000000 INFO @ Mon, 03 Jun 2019 15:08:04: 16000000 INFO @ Mon, 03 Jun 2019 15:08:10: 18000000 INFO @ Mon, 03 Jun 2019 15:08:10: 17000000 INFO @ Mon, 03 Jun 2019 15:08:13: 17000000 INFO @ Mon, 03 Jun 2019 15:08:18: 19000000 INFO @ Mon, 03 Jun 2019 15:08:19: 18000000 INFO @ Mon, 03 Jun 2019 15:08:21: 18000000 INFO @ Mon, 03 Jun 2019 15:08:26: 20000000 INFO @ Mon, 03 Jun 2019 15:08:27: 19000000 INFO @ Mon, 03 Jun 2019 15:08:29: 19000000 INFO @ Mon, 03 Jun 2019 15:08:34: 21000000 INFO @ Mon, 03 Jun 2019 15:08:36: 20000000 INFO @ Mon, 03 Jun 2019 15:08:37: 20000000 INFO @ Mon, 03 Jun 2019 15:08:43: 22000000 INFO @ Mon, 03 Jun 2019 15:08:45: 21000000 INFO @ Mon, 03 Jun 2019 15:08:46: 21000000 INFO @ Mon, 03 Jun 2019 15:08:52: 23000000 INFO @ Mon, 03 Jun 2019 15:08:53: 22000000 INFO @ Mon, 03 Jun 2019 15:08:55: 22000000 INFO @ Mon, 03 Jun 2019 15:09:02: 24000000 INFO @ Mon, 03 Jun 2019 15:09:02: 23000000 INFO @ Mon, 03 Jun 2019 15:09:05: 23000000 INFO @ Mon, 03 Jun 2019 15:09:11: 24000000 INFO @ Mon, 03 Jun 2019 15:09:12: 25000000 INFO @ Mon, 03 Jun 2019 15:09:14: 24000000 INFO @ Mon, 03 Jun 2019 15:09:20: 25000000 INFO @ Mon, 03 Jun 2019 15:09:21: 26000000 INFO @ Mon, 03 Jun 2019 15:09:23: 25000000 INFO @ Mon, 03 Jun 2019 15:09:28: 26000000 INFO @ Mon, 03 Jun 2019 15:09:28: 27000000 INFO @ Mon, 03 Jun 2019 15:09:32: 26000000 INFO @ Mon, 03 Jun 2019 15:09:37: 27000000 INFO @ Mon, 03 Jun 2019 15:09:37: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 15:09:37: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 15:09:37: #1 total tags in treatment: 27927033 INFO @ Mon, 03 Jun 2019 15:09:37: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 15:09:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 15:09:37: #1 tags after filtering in treatment: 27927033 INFO @ Mon, 03 Jun 2019 15:09:37: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 15:09:37: #1 finished! INFO @ Mon, 03 Jun 2019 15:09:37: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 15:09:37: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 15:09:40: #2 number of paired peaks: 40 WARNING @ Mon, 03 Jun 2019 15:09:40: Too few paired peaks (40) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 15:09:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX375352/SRX375352.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX375352/SRX375352.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX375352/SRX375352.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX375352/SRX375352.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 15:09:41: 27000000 INFO @ Mon, 03 Jun 2019 15:09:45: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 15:09:45: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 15:09:45: #1 total tags in treatment: 27927033 INFO @ Mon, 03 Jun 2019 15:09:45: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 15:09:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 15:09:46: #1 tags after filtering in treatment: 27927033 INFO @ Mon, 03 Jun 2019 15:09:46: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 15:09:46: #1 finished! INFO @ Mon, 03 Jun 2019 15:09:46: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 15:09:46: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 15:09:48: #2 number of paired peaks: 40 WARNING @ Mon, 03 Jun 2019 15:09:48: Too few paired peaks (40) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 15:09:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX375352/SRX375352.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX375352/SRX375352.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX375352/SRX375352.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX375352/SRX375352.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 15:09:50: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 15:09:50: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 15:09:50: #1 total tags in treatment: 27927033 INFO @ Mon, 03 Jun 2019 15:09:50: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 15:09:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 15:09:51: #1 tags after filtering in treatment: 27927033 INFO @ Mon, 03 Jun 2019 15:09:51: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 15:09:51: #1 finished! INFO @ Mon, 03 Jun 2019 15:09:51: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 15:09:51: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 15:09:53: #2 number of paired peaks: 40 WARNING @ Mon, 03 Jun 2019 15:09:53: Too few paired peaks (40) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 15:09:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX375352/SRX375352.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX375352/SRX375352.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX375352/SRX375352.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX375352/SRX375352.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。