Job ID = 6528000 SRX = SRX3663167 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:18:20 prefetch.2.10.7: 1) Downloading 'SRR6686977'... 2020-06-29T14:18:20 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:21:16 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:21:16 prefetch.2.10.7: 1) 'SRR6686977' was downloaded successfully Read 28508523 spots for SRR6686977/SRR6686977.sra Written 28508523 spots for SRR6686977/SRR6686977.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:08 28508523 reads; of these: 28508523 (100.00%) were unpaired; of these: 4233429 (14.85%) aligned 0 times 20646164 (72.42%) aligned exactly 1 time 3628930 (12.73%) aligned >1 times 85.15% overall alignment rate Time searching: 00:07:08 Overall time: 00:07:08 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 1973892 / 24275094 = 0.0813 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:47:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3663167/SRX3663167.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3663167/SRX3663167.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3663167/SRX3663167.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3663167/SRX3663167.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:47:08: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:47:08: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:47:14: 1000000 INFO @ Mon, 29 Jun 2020 23:47:20: 2000000 INFO @ Mon, 29 Jun 2020 23:47:27: 3000000 INFO @ Mon, 29 Jun 2020 23:47:33: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:47:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3663167/SRX3663167.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3663167/SRX3663167.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3663167/SRX3663167.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3663167/SRX3663167.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:47:38: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:47:38: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:47:39: 5000000 INFO @ Mon, 29 Jun 2020 23:47:45: 1000000 INFO @ Mon, 29 Jun 2020 23:47:45: 6000000 INFO @ Mon, 29 Jun 2020 23:47:51: 2000000 INFO @ Mon, 29 Jun 2020 23:47:52: 7000000 INFO @ Mon, 29 Jun 2020 23:47:58: 3000000 INFO @ Mon, 29 Jun 2020 23:47:58: 8000000 INFO @ Mon, 29 Jun 2020 23:48:04: 9000000 INFO @ Mon, 29 Jun 2020 23:48:04: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:48:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3663167/SRX3663167.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3663167/SRX3663167.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3663167/SRX3663167.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3663167/SRX3663167.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:48:08: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:48:08: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:48:11: 10000000 INFO @ Mon, 29 Jun 2020 23:48:11: 5000000 INFO @ Mon, 29 Jun 2020 23:48:15: 1000000 INFO @ Mon, 29 Jun 2020 23:48:17: 11000000 INFO @ Mon, 29 Jun 2020 23:48:17: 6000000 INFO @ Mon, 29 Jun 2020 23:48:21: 2000000 INFO @ Mon, 29 Jun 2020 23:48:24: 12000000 INFO @ Mon, 29 Jun 2020 23:48:24: 7000000 INFO @ Mon, 29 Jun 2020 23:48:28: 3000000 INFO @ Mon, 29 Jun 2020 23:48:31: 8000000 INFO @ Mon, 29 Jun 2020 23:48:31: 13000000 INFO @ Mon, 29 Jun 2020 23:48:35: 4000000 INFO @ Mon, 29 Jun 2020 23:48:37: 9000000 INFO @ Mon, 29 Jun 2020 23:48:37: 14000000 INFO @ Mon, 29 Jun 2020 23:48:41: 5000000 INFO @ Mon, 29 Jun 2020 23:48:44: 10000000 INFO @ Mon, 29 Jun 2020 23:48:44: 15000000 INFO @ Mon, 29 Jun 2020 23:48:48: 6000000 INFO @ Mon, 29 Jun 2020 23:48:50: 11000000 INFO @ Mon, 29 Jun 2020 23:48:51: 16000000 INFO @ Mon, 29 Jun 2020 23:48:55: 7000000 INFO @ Mon, 29 Jun 2020 23:48:57: 12000000 INFO @ Mon, 29 Jun 2020 23:48:57: 17000000 INFO @ Mon, 29 Jun 2020 23:49:01: 8000000 INFO @ Mon, 29 Jun 2020 23:49:03: 13000000 INFO @ Mon, 29 Jun 2020 23:49:04: 18000000 INFO @ Mon, 29 Jun 2020 23:49:08: 9000000 INFO @ Mon, 29 Jun 2020 23:49:10: 14000000 INFO @ Mon, 29 Jun 2020 23:49:11: 19000000 INFO @ Mon, 29 Jun 2020 23:49:15: 10000000 INFO @ Mon, 29 Jun 2020 23:49:16: 15000000 INFO @ Mon, 29 Jun 2020 23:49:18: 20000000 INFO @ Mon, 29 Jun 2020 23:49:21: 11000000 INFO @ Mon, 29 Jun 2020 23:49:23: 16000000 INFO @ Mon, 29 Jun 2020 23:49:24: 21000000 INFO @ Mon, 29 Jun 2020 23:49:28: 12000000 INFO @ Mon, 29 Jun 2020 23:49:30: 17000000 INFO @ Mon, 29 Jun 2020 23:49:31: 22000000 INFO @ Mon, 29 Jun 2020 23:49:33: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:49:33: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:49:33: #1 total tags in treatment: 22301202 INFO @ Mon, 29 Jun 2020 23:49:33: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:49:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:49:34: #1 tags after filtering in treatment: 22301202 INFO @ Mon, 29 Jun 2020 23:49:34: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:49:34: #1 finished! INFO @ Mon, 29 Jun 2020 23:49:34: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:49:34: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:49:34: 13000000 INFO @ Mon, 29 Jun 2020 23:49:35: #2 number of paired peaks: 73 WARNING @ Mon, 29 Jun 2020 23:49:35: Too few paired peaks (73) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:49:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3663167/SRX3663167.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3663167/SRX3663167.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3663167/SRX3663167.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3663167/SRX3663167.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:49:36: 18000000 INFO @ Mon, 29 Jun 2020 23:49:41: 14000000 INFO @ Mon, 29 Jun 2020 23:49:43: 19000000 INFO @ Mon, 29 Jun 2020 23:49:47: 15000000 INFO @ Mon, 29 Jun 2020 23:49:50: 20000000 INFO @ Mon, 29 Jun 2020 23:49:54: 16000000 INFO @ Mon, 29 Jun 2020 23:49:57: 21000000 INFO @ Mon, 29 Jun 2020 23:50:01: 17000000 INFO @ Mon, 29 Jun 2020 23:50:03: 22000000 INFO @ Mon, 29 Jun 2020 23:50:06: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:50:06: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:50:06: #1 total tags in treatment: 22301202 INFO @ Mon, 29 Jun 2020 23:50:06: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:50:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:50:06: #1 tags after filtering in treatment: 22301202 INFO @ Mon, 29 Jun 2020 23:50:06: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:50:06: #1 finished! INFO @ Mon, 29 Jun 2020 23:50:06: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:50:06: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:50:08: #2 number of paired peaks: 73 WARNING @ Mon, 29 Jun 2020 23:50:08: Too few paired peaks (73) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:50:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3663167/SRX3663167.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3663167/SRX3663167.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3663167/SRX3663167.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3663167/SRX3663167.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:50:08: 18000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 23:50:15: 19000000 INFO @ Mon, 29 Jun 2020 23:50:22: 20000000 INFO @ Mon, 29 Jun 2020 23:50:29: 21000000 INFO @ Mon, 29 Jun 2020 23:50:36: 22000000 INFO @ Mon, 29 Jun 2020 23:50:38: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:50:38: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:50:38: #1 total tags in treatment: 22301202 INFO @ Mon, 29 Jun 2020 23:50:38: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:50:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:50:39: #1 tags after filtering in treatment: 22301202 INFO @ Mon, 29 Jun 2020 23:50:39: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:50:39: #1 finished! INFO @ Mon, 29 Jun 2020 23:50:39: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:50:39: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:50:40: #2 number of paired peaks: 73 WARNING @ Mon, 29 Jun 2020 23:50:40: Too few paired peaks (73) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:50:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3663167/SRX3663167.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3663167/SRX3663167.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3663167/SRX3663167.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3663167/SRX3663167.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。