Job ID = 6527999 SRX = SRX3663166 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:03:07 prefetch.2.10.7: 1) Downloading 'SRR6686976'... 2020-06-29T14:03:07 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:07:43 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:07:43 prefetch.2.10.7: 1) 'SRR6686976' was downloaded successfully Read 27154552 spots for SRR6686976/SRR6686976.sra Written 27154552 spots for SRR6686976/SRR6686976.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:07 27154552 reads; of these: 27154552 (100.00%) were unpaired; of these: 4341379 (15.99%) aligned 0 times 20134231 (74.15%) aligned exactly 1 time 2678942 (9.87%) aligned >1 times 84.01% overall alignment rate Time searching: 00:06:08 Overall time: 00:06:08 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 3390138 / 22813173 = 0.1486 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:29:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3663166/SRX3663166.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3663166/SRX3663166.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3663166/SRX3663166.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3663166/SRX3663166.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:29:16: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:29:16: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:29:22: 1000000 INFO @ Mon, 29 Jun 2020 23:29:28: 2000000 INFO @ Mon, 29 Jun 2020 23:29:34: 3000000 INFO @ Mon, 29 Jun 2020 23:29:40: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:29:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3663166/SRX3663166.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3663166/SRX3663166.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3663166/SRX3663166.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3663166/SRX3663166.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:29:46: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:29:46: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:29:46: 5000000 INFO @ Mon, 29 Jun 2020 23:29:53: 1000000 INFO @ Mon, 29 Jun 2020 23:29:53: 6000000 INFO @ Mon, 29 Jun 2020 23:29:59: 2000000 INFO @ Mon, 29 Jun 2020 23:30:00: 7000000 INFO @ Mon, 29 Jun 2020 23:30:06: 8000000 INFO @ Mon, 29 Jun 2020 23:30:06: 3000000 INFO @ Mon, 29 Jun 2020 23:30:13: 9000000 INFO @ Mon, 29 Jun 2020 23:30:13: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:30:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3663166/SRX3663166.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3663166/SRX3663166.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3663166/SRX3663166.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3663166/SRX3663166.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:30:16: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:30:16: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:30:20: 10000000 INFO @ Mon, 29 Jun 2020 23:30:20: 5000000 INFO @ Mon, 29 Jun 2020 23:30:23: 1000000 INFO @ Mon, 29 Jun 2020 23:30:27: 6000000 INFO @ Mon, 29 Jun 2020 23:30:27: 11000000 INFO @ Mon, 29 Jun 2020 23:30:30: 2000000 INFO @ Mon, 29 Jun 2020 23:30:34: 12000000 INFO @ Mon, 29 Jun 2020 23:30:34: 7000000 INFO @ Mon, 29 Jun 2020 23:30:37: 3000000 INFO @ Mon, 29 Jun 2020 23:30:41: 8000000 INFO @ Mon, 29 Jun 2020 23:30:41: 13000000 INFO @ Mon, 29 Jun 2020 23:30:44: 4000000 INFO @ Mon, 29 Jun 2020 23:30:47: 9000000 INFO @ Mon, 29 Jun 2020 23:30:48: 14000000 INFO @ Mon, 29 Jun 2020 23:30:51: 5000000 INFO @ Mon, 29 Jun 2020 23:30:55: 10000000 INFO @ Mon, 29 Jun 2020 23:30:55: 15000000 INFO @ Mon, 29 Jun 2020 23:30:58: 6000000 INFO @ Mon, 29 Jun 2020 23:31:02: 11000000 INFO @ Mon, 29 Jun 2020 23:31:02: 16000000 INFO @ Mon, 29 Jun 2020 23:31:05: 7000000 INFO @ Mon, 29 Jun 2020 23:31:09: 12000000 INFO @ Mon, 29 Jun 2020 23:31:09: 17000000 INFO @ Mon, 29 Jun 2020 23:31:12: 8000000 INFO @ Mon, 29 Jun 2020 23:31:15: 13000000 INFO @ Mon, 29 Jun 2020 23:31:16: 18000000 INFO @ Mon, 29 Jun 2020 23:31:18: 9000000 INFO @ Mon, 29 Jun 2020 23:31:22: 14000000 INFO @ Mon, 29 Jun 2020 23:31:23: 19000000 INFO @ Mon, 29 Jun 2020 23:31:25: 10000000 INFO @ Mon, 29 Jun 2020 23:31:26: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:31:26: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:31:26: #1 total tags in treatment: 19423035 INFO @ Mon, 29 Jun 2020 23:31:26: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:31:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:31:26: #1 tags after filtering in treatment: 19423035 INFO @ Mon, 29 Jun 2020 23:31:26: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:31:26: #1 finished! INFO @ Mon, 29 Jun 2020 23:31:26: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:31:26: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:31:27: #2 number of paired peaks: 71 WARNING @ Mon, 29 Jun 2020 23:31:27: Too few paired peaks (71) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:31:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3663166/SRX3663166.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3663166/SRX3663166.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3663166/SRX3663166.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3663166/SRX3663166.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:31:29: 15000000 INFO @ Mon, 29 Jun 2020 23:31:32: 11000000 INFO @ Mon, 29 Jun 2020 23:31:37: 16000000 INFO @ Mon, 29 Jun 2020 23:31:39: 12000000 INFO @ Mon, 29 Jun 2020 23:31:43: 17000000 INFO @ Mon, 29 Jun 2020 23:31:46: 13000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 23:31:50: 18000000 INFO @ Mon, 29 Jun 2020 23:31:52: 14000000 INFO @ Mon, 29 Jun 2020 23:31:57: 19000000 INFO @ Mon, 29 Jun 2020 23:31:59: 15000000 INFO @ Mon, 29 Jun 2020 23:32:00: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:32:00: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:32:00: #1 total tags in treatment: 19423035 INFO @ Mon, 29 Jun 2020 23:32:00: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:32:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:32:00: #1 tags after filtering in treatment: 19423035 INFO @ Mon, 29 Jun 2020 23:32:00: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:32:00: #1 finished! INFO @ Mon, 29 Jun 2020 23:32:00: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:32:00: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:32:01: #2 number of paired peaks: 71 WARNING @ Mon, 29 Jun 2020 23:32:01: Too few paired peaks (71) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:32:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3663166/SRX3663166.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3663166/SRX3663166.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3663166/SRX3663166.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3663166/SRX3663166.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:32:06: 16000000 INFO @ Mon, 29 Jun 2020 23:32:12: 17000000 INFO @ Mon, 29 Jun 2020 23:32:18: 18000000 INFO @ Mon, 29 Jun 2020 23:32:24: 19000000 INFO @ Mon, 29 Jun 2020 23:32:27: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:32:27: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:32:27: #1 total tags in treatment: 19423035 INFO @ Mon, 29 Jun 2020 23:32:27: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:32:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:32:27: #1 tags after filtering in treatment: 19423035 INFO @ Mon, 29 Jun 2020 23:32:27: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:32:27: #1 finished! INFO @ Mon, 29 Jun 2020 23:32:27: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:32:27: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:32:29: #2 number of paired peaks: 71 WARNING @ Mon, 29 Jun 2020 23:32:29: Too few paired peaks (71) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:32:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3663166/SRX3663166.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3663166/SRX3663166.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3663166/SRX3663166.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3663166/SRX3663166.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。