Job ID = 6527993 SRX = SRX3663160 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:02:21 prefetch.2.10.7: 1) Downloading 'SRR6686970'... 2020-06-29T14:02:21 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:08:05 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:08:05 prefetch.2.10.7: 1) 'SRR6686970' was downloaded successfully Read 25047914 spots for SRR6686970/SRR6686970.sra Written 25047914 spots for SRR6686970/SRR6686970.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:26 25047914 reads; of these: 25047914 (100.00%) were unpaired; of these: 1661437 (6.63%) aligned 0 times 19839407 (79.21%) aligned exactly 1 time 3547070 (14.16%) aligned >1 times 93.37% overall alignment rate Time searching: 00:06:26 Overall time: 00:06:26 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 2618529 / 23386477 = 0.1120 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:29:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3663160/SRX3663160.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3663160/SRX3663160.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3663160/SRX3663160.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3663160/SRX3663160.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:29:24: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:29:24: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:29:30: 1000000 INFO @ Mon, 29 Jun 2020 23:29:36: 2000000 INFO @ Mon, 29 Jun 2020 23:29:42: 3000000 INFO @ Mon, 29 Jun 2020 23:29:48: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:29:53: 5000000 INFO @ Mon, 29 Jun 2020 23:29:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3663160/SRX3663160.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3663160/SRX3663160.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3663160/SRX3663160.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3663160/SRX3663160.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:29:54: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:29:54: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:29:59: 1000000 INFO @ Mon, 29 Jun 2020 23:29:59: 6000000 INFO @ Mon, 29 Jun 2020 23:30:05: 2000000 INFO @ Mon, 29 Jun 2020 23:30:05: 7000000 INFO @ Mon, 29 Jun 2020 23:30:10: 3000000 INFO @ Mon, 29 Jun 2020 23:30:11: 8000000 INFO @ Mon, 29 Jun 2020 23:30:15: 4000000 INFO @ Mon, 29 Jun 2020 23:30:17: 9000000 INFO @ Mon, 29 Jun 2020 23:30:21: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:30:23: 10000000 INFO @ Mon, 29 Jun 2020 23:30:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3663160/SRX3663160.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3663160/SRX3663160.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3663160/SRX3663160.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3663160/SRX3663160.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:30:24: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:30:24: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:30:26: 6000000 INFO @ Mon, 29 Jun 2020 23:30:29: 11000000 INFO @ Mon, 29 Jun 2020 23:30:29: 1000000 INFO @ Mon, 29 Jun 2020 23:30:32: 7000000 INFO @ Mon, 29 Jun 2020 23:30:35: 2000000 INFO @ Mon, 29 Jun 2020 23:30:35: 12000000 INFO @ Mon, 29 Jun 2020 23:30:37: 8000000 INFO @ Mon, 29 Jun 2020 23:30:41: 3000000 INFO @ Mon, 29 Jun 2020 23:30:41: 13000000 INFO @ Mon, 29 Jun 2020 23:30:42: 9000000 INFO @ Mon, 29 Jun 2020 23:30:46: 4000000 INFO @ Mon, 29 Jun 2020 23:30:47: 14000000 INFO @ Mon, 29 Jun 2020 23:30:48: 10000000 INFO @ Mon, 29 Jun 2020 23:30:52: 5000000 INFO @ Mon, 29 Jun 2020 23:30:53: 15000000 INFO @ Mon, 29 Jun 2020 23:30:53: 11000000 INFO @ Mon, 29 Jun 2020 23:30:57: 6000000 INFO @ Mon, 29 Jun 2020 23:30:59: 12000000 INFO @ Mon, 29 Jun 2020 23:30:59: 16000000 INFO @ Mon, 29 Jun 2020 23:31:03: 7000000 INFO @ Mon, 29 Jun 2020 23:31:04: 13000000 INFO @ Mon, 29 Jun 2020 23:31:05: 17000000 INFO @ Mon, 29 Jun 2020 23:31:08: 8000000 INFO @ Mon, 29 Jun 2020 23:31:10: 14000000 INFO @ Mon, 29 Jun 2020 23:31:11: 18000000 INFO @ Mon, 29 Jun 2020 23:31:13: 9000000 INFO @ Mon, 29 Jun 2020 23:31:15: 15000000 INFO @ Mon, 29 Jun 2020 23:31:17: 19000000 INFO @ Mon, 29 Jun 2020 23:31:19: 10000000 INFO @ Mon, 29 Jun 2020 23:31:21: 16000000 INFO @ Mon, 29 Jun 2020 23:31:23: 20000000 INFO @ Mon, 29 Jun 2020 23:31:24: 11000000 INFO @ Mon, 29 Jun 2020 23:31:26: 17000000 INFO @ Mon, 29 Jun 2020 23:31:28: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:31:28: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:31:28: #1 total tags in treatment: 20767948 INFO @ Mon, 29 Jun 2020 23:31:28: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:31:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:31:28: #1 tags after filtering in treatment: 20767948 INFO @ Mon, 29 Jun 2020 23:31:28: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:31:28: #1 finished! INFO @ Mon, 29 Jun 2020 23:31:28: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:31:28: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:31:29: #2 number of paired peaks: 96 WARNING @ Mon, 29 Jun 2020 23:31:29: Too few paired peaks (96) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:31:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3663160/SRX3663160.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3663160/SRX3663160.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3663160/SRX3663160.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3663160/SRX3663160.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:31:30: 12000000 INFO @ Mon, 29 Jun 2020 23:31:32: 18000000 INFO @ Mon, 29 Jun 2020 23:31:35: 13000000 INFO @ Mon, 29 Jun 2020 23:31:37: 19000000 INFO @ Mon, 29 Jun 2020 23:31:40: 14000000 INFO @ Mon, 29 Jun 2020 23:31:42: 20000000 INFO @ Mon, 29 Jun 2020 23:31:46: 15000000 INFO @ Mon, 29 Jun 2020 23:31:47: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:31:47: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:31:47: #1 total tags in treatment: 20767948 INFO @ Mon, 29 Jun 2020 23:31:47: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:31:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:31:47: #1 tags after filtering in treatment: 20767948 INFO @ Mon, 29 Jun 2020 23:31:47: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:31:47: #1 finished! INFO @ Mon, 29 Jun 2020 23:31:47: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:31:47: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:31:48: #2 number of paired peaks: 96 WARNING @ Mon, 29 Jun 2020 23:31:48: Too few paired peaks (96) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:31:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3663160/SRX3663160.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3663160/SRX3663160.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3663160/SRX3663160.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3663160/SRX3663160.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:31:51: 16000000 INFO @ Mon, 29 Jun 2020 23:31:56: 17000000 INFO @ Mon, 29 Jun 2020 23:32:02: 18000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 23:32:07: 19000000 INFO @ Mon, 29 Jun 2020 23:32:12: 20000000 INFO @ Mon, 29 Jun 2020 23:32:16: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:32:16: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:32:16: #1 total tags in treatment: 20767948 INFO @ Mon, 29 Jun 2020 23:32:16: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:32:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:32:16: #1 tags after filtering in treatment: 20767948 INFO @ Mon, 29 Jun 2020 23:32:16: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:32:16: #1 finished! INFO @ Mon, 29 Jun 2020 23:32:16: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:32:16: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:32:18: #2 number of paired peaks: 96 WARNING @ Mon, 29 Jun 2020 23:32:18: Too few paired peaks (96) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:32:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3663160/SRX3663160.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3663160/SRX3663160.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3663160/SRX3663160.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3663160/SRX3663160.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。