Job ID = 11240720 sra ファイルのダウンロード中... Completed: 1528989K bytes transferred in 71 seconds (174661K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 74853287 spots for /home/okishinya/chipatlas/results/dm3/SRX3630329/SRR6652851.sra Written 74853287 spots for /home/okishinya/chipatlas/results/dm3/SRX3630329/SRR6652851.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:27:35 74853287 reads; of these: 74853287 (100.00%) were unpaired; of these: 3634650 (4.86%) aligned 0 times 55536030 (74.19%) aligned exactly 1 time 15682607 (20.95%) aligned >1 times 95.14% overall alignment rate Time searching: 00:27:35 Overall time: 00:27:36 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 32 files... [bam_rmdupse_core] 19240789 / 71218637 = 0.2702 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sun, 07 Oct 2018 21:19:16: # Command line: callpeak -t SRX3630329.bam -f BAM -g dm -n SRX3630329.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX3630329.05 # format = BAM # ChIP-seq file = ['SRX3630329.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 07 Oct 2018 21:19:16: #1 read tag files... INFO @ Sun, 07 Oct 2018 21:19:16: # Command line: callpeak -t SRX3630329.bam -f BAM -g dm -n SRX3630329.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX3630329.20 # format = BAM # ChIP-seq file = ['SRX3630329.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 07 Oct 2018 21:19:16: #1 read treatment tags... INFO @ Sun, 07 Oct 2018 21:19:16: #1 read tag files... INFO @ Sun, 07 Oct 2018 21:19:16: #1 read treatment tags... INFO @ Sun, 07 Oct 2018 21:19:16: # Command line: callpeak -t SRX3630329.bam -f BAM -g dm -n SRX3630329.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX3630329.10 # format = BAM # ChIP-seq file = ['SRX3630329.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 07 Oct 2018 21:19:16: #1 read tag files... INFO @ Sun, 07 Oct 2018 21:19:16: #1 read treatment tags... INFO @ Sun, 07 Oct 2018 21:19:22: 1000000 INFO @ Sun, 07 Oct 2018 21:19:22: 1000000 INFO @ Sun, 07 Oct 2018 21:19:23: 1000000 INFO @ Sun, 07 Oct 2018 21:19:27: 2000000 INFO @ Sun, 07 Oct 2018 21:19:29: 2000000 INFO @ Sun, 07 Oct 2018 21:19:29: 2000000 INFO @ Sun, 07 Oct 2018 21:19:33: 3000000 INFO @ Sun, 07 Oct 2018 21:19:35: 3000000 INFO @ Sun, 07 Oct 2018 21:19:35: 3000000 INFO @ Sun, 07 Oct 2018 21:19:39: 4000000 INFO @ Sun, 07 Oct 2018 21:19:41: 4000000 INFO @ Sun, 07 Oct 2018 21:19:42: 4000000 INFO @ Sun, 07 Oct 2018 21:19:45: 5000000 INFO @ Sun, 07 Oct 2018 21:19:47: 5000000 INFO @ Sun, 07 Oct 2018 21:19:48: 5000000 INFO @ Sun, 07 Oct 2018 21:19:50: 6000000 INFO @ Sun, 07 Oct 2018 21:19:54: 6000000 INFO @ Sun, 07 Oct 2018 21:19:55: 6000000 INFO @ Sun, 07 Oct 2018 21:19:56: 7000000 INFO @ Sun, 07 Oct 2018 21:20:02: 7000000 INFO @ Sun, 07 Oct 2018 21:20:02: 8000000 INFO @ Sun, 07 Oct 2018 21:20:03: 7000000 INFO @ Sun, 07 Oct 2018 21:20:08: 9000000 INFO @ Sun, 07 Oct 2018 21:20:09: 8000000 INFO @ Sun, 07 Oct 2018 21:20:11: 8000000 INFO @ Sun, 07 Oct 2018 21:20:13: 10000000 INFO @ Sun, 07 Oct 2018 21:20:18: 9000000 INFO @ Sun, 07 Oct 2018 21:20:19: 9000000 INFO @ Sun, 07 Oct 2018 21:20:19: 11000000 INFO @ Sun, 07 Oct 2018 21:20:25: 12000000 INFO @ Sun, 07 Oct 2018 21:20:26: 10000000 INFO @ Sun, 07 Oct 2018 21:20:27: 10000000 INFO @ Sun, 07 Oct 2018 21:20:30: 13000000 INFO @ Sun, 07 Oct 2018 21:20:34: 11000000 INFO @ Sun, 07 Oct 2018 21:20:34: 11000000 INFO @ Sun, 07 Oct 2018 21:20:36: 14000000 INFO @ Sun, 07 Oct 2018 21:20:42: 15000000 INFO @ Sun, 07 Oct 2018 21:20:42: 12000000 INFO @ Sun, 07 Oct 2018 21:20:42: 12000000 INFO @ Sun, 07 Oct 2018 21:20:48: 16000000 INFO @ Sun, 07 Oct 2018 21:20:49: 13000000 INFO @ Sun, 07 Oct 2018 21:20:49: 13000000 INFO @ Sun, 07 Oct 2018 21:20:54: 17000000 INFO @ Sun, 07 Oct 2018 21:20:55: 14000000 INFO @ Sun, 07 Oct 2018 21:20:56: 14000000 INFO @ Sun, 07 Oct 2018 21:21:00: 18000000 INFO @ Sun, 07 Oct 2018 21:21:02: 15000000 INFO @ Sun, 07 Oct 2018 21:21:03: 15000000 INFO @ Sun, 07 Oct 2018 21:21:06: 19000000 INFO @ Sun, 07 Oct 2018 21:21:09: 16000000 INFO @ Sun, 07 Oct 2018 21:21:10: 16000000 INFO @ Sun, 07 Oct 2018 21:21:12: 20000000 INFO @ Sun, 07 Oct 2018 21:21:16: 17000000 INFO @ Sun, 07 Oct 2018 21:21:17: 17000000 INFO @ Sun, 07 Oct 2018 21:21:18: 21000000 INFO @ Sun, 07 Oct 2018 21:21:23: 18000000 INFO @ Sun, 07 Oct 2018 21:21:24: 18000000 INFO @ Sun, 07 Oct 2018 21:21:24: 22000000 INFO @ Sun, 07 Oct 2018 21:21:29: 19000000 INFO @ Sun, 07 Oct 2018 21:21:30: 23000000 INFO @ Sun, 07 Oct 2018 21:21:31: 19000000 INFO @ Sun, 07 Oct 2018 21:21:36: 24000000 INFO @ Sun, 07 Oct 2018 21:21:37: 20000000 INFO @ Sun, 07 Oct 2018 21:21:39: 20000000 INFO @ Sun, 07 Oct 2018 21:21:41: 25000000 INFO @ Sun, 07 Oct 2018 21:21:46: 21000000 INFO @ Sun, 07 Oct 2018 21:21:46: 21000000 INFO @ Sun, 07 Oct 2018 21:21:47: 26000000 INFO @ Sun, 07 Oct 2018 21:21:53: 27000000 INFO @ Sun, 07 Oct 2018 21:21:53: 22000000 INFO @ Sun, 07 Oct 2018 21:21:54: 22000000 INFO @ Sun, 07 Oct 2018 21:21:59: 28000000 INFO @ Sun, 07 Oct 2018 21:22:01: 23000000 INFO @ Sun, 07 Oct 2018 21:22:01: 23000000 INFO @ Sun, 07 Oct 2018 21:22:04: 29000000 INFO @ Sun, 07 Oct 2018 21:22:09: 24000000 INFO @ Sun, 07 Oct 2018 21:22:09: 24000000 INFO @ Sun, 07 Oct 2018 21:22:10: 30000000 INFO @ Sun, 07 Oct 2018 21:22:16: 31000000 INFO @ Sun, 07 Oct 2018 21:22:17: 25000000 INFO @ Sun, 07 Oct 2018 21:22:17: 25000000 INFO @ Sun, 07 Oct 2018 21:22:22: 32000000 INFO @ Sun, 07 Oct 2018 21:22:24: 26000000 INFO @ Sun, 07 Oct 2018 21:22:24: 26000000 INFO @ Sun, 07 Oct 2018 21:22:29: 33000000 INFO @ Sun, 07 Oct 2018 21:22:30: 27000000 INFO @ Sun, 07 Oct 2018 21:22:31: 27000000 INFO @ Sun, 07 Oct 2018 21:22:35: 34000000 INFO @ Sun, 07 Oct 2018 21:22:36: 28000000 INFO @ Sun, 07 Oct 2018 21:22:38: 28000000 INFO @ Sun, 07 Oct 2018 21:22:42: 29000000 INFO @ Sun, 07 Oct 2018 21:22:43: 35000000 INFO @ Sun, 07 Oct 2018 21:22:46: 29000000 INFO @ Sun, 07 Oct 2018 21:22:49: 30000000 INFO @ Sun, 07 Oct 2018 21:22:50: 36000000 INFO @ Sun, 07 Oct 2018 21:22:54: 30000000 INFO @ Sun, 07 Oct 2018 21:22:54: 31000000 INFO @ Sun, 07 Oct 2018 21:22:57: 37000000 INFO @ Sun, 07 Oct 2018 21:23:00: 31000000 INFO @ Sun, 07 Oct 2018 21:23:01: 32000000 INFO @ Sun, 07 Oct 2018 21:23:03: 38000000 INFO @ Sun, 07 Oct 2018 21:23:07: 32000000 INFO @ Sun, 07 Oct 2018 21:23:07: 33000000 INFO @ Sun, 07 Oct 2018 21:23:10: 39000000 INFO @ Sun, 07 Oct 2018 21:23:13: 34000000 INFO @ Sun, 07 Oct 2018 21:23:14: 33000000 INFO @ Sun, 07 Oct 2018 21:23:17: 40000000 INFO @ Sun, 07 Oct 2018 21:23:19: 35000000 INFO @ Sun, 07 Oct 2018 21:23:22: 34000000 INFO @ Sun, 07 Oct 2018 21:23:24: 41000000 INFO @ Sun, 07 Oct 2018 21:23:26: 36000000 INFO @ Sun, 07 Oct 2018 21:23:30: 35000000 INFO @ Sun, 07 Oct 2018 21:23:32: 37000000 INFO @ Sun, 07 Oct 2018 21:23:32: 42000000 INFO @ Sun, 07 Oct 2018 21:23:37: 36000000 INFO @ Sun, 07 Oct 2018 21:23:38: 38000000 INFO @ Sun, 07 Oct 2018 21:23:38: 43000000 INFO @ Sun, 07 Oct 2018 21:23:44: 39000000 INFO @ Sun, 07 Oct 2018 21:23:44: 37000000 INFO @ Sun, 07 Oct 2018 21:23:46: 44000000 INFO @ Sun, 07 Oct 2018 21:23:50: 40000000 INFO @ Sun, 07 Oct 2018 21:23:52: 38000000 INFO @ Sun, 07 Oct 2018 21:23:53: 45000000 INFO @ Sun, 07 Oct 2018 21:23:56: 41000000 INFO @ Sun, 07 Oct 2018 21:24:00: 39000000 INFO @ Sun, 07 Oct 2018 21:24:00: 46000000 INFO @ Sun, 07 Oct 2018 21:24:02: 42000000 INFO @ Sun, 07 Oct 2018 21:24:07: 40000000 INFO @ Sun, 07 Oct 2018 21:24:07: 47000000 INFO @ Sun, 07 Oct 2018 21:24:08: 43000000 INFO @ Sun, 07 Oct 2018 21:24:14: 44000000 INFO @ Sun, 07 Oct 2018 21:24:15: 41000000 INFO @ Sun, 07 Oct 2018 21:24:15: 48000000 INFO @ Sun, 07 Oct 2018 21:24:20: 45000000 INFO @ Sun, 07 Oct 2018 21:24:23: 42000000 INFO @ Sun, 07 Oct 2018 21:24:23: 49000000 INFO @ Sun, 07 Oct 2018 21:24:26: 46000000 INFO @ Sun, 07 Oct 2018 21:24:29: 50000000 INFO @ Sun, 07 Oct 2018 21:24:30: 43000000 INFO @ Sun, 07 Oct 2018 21:24:32: 47000000 INFO @ Sun, 07 Oct 2018 21:24:37: 51000000 INFO @ Sun, 07 Oct 2018 21:24:37: 44000000 INFO @ Sun, 07 Oct 2018 21:24:38: 48000000 INFO @ Sun, 07 Oct 2018 21:24:44: 49000000 INFO @ Sun, 07 Oct 2018 21:24:45: #1 tag size is determined as 51 bps INFO @ Sun, 07 Oct 2018 21:24:45: #1 tag size = 51 INFO @ Sun, 07 Oct 2018 21:24:45: #1 total tags in treatment: 51977848 INFO @ Sun, 07 Oct 2018 21:24:45: #1 user defined the maximum tags... INFO @ Sun, 07 Oct 2018 21:24:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 07 Oct 2018 21:24:45: 45000000 INFO @ Sun, 07 Oct 2018 21:24:46: #1 tags after filtering in treatment: 51977848 INFO @ Sun, 07 Oct 2018 21:24:46: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 07 Oct 2018 21:24:46: #1 finished! INFO @ Sun, 07 Oct 2018 21:24:46: #2 Build Peak Model... INFO @ Sun, 07 Oct 2018 21:24:46: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 07 Oct 2018 21:24:50: #2 number of paired peaks: 0 WARNING @ Sun, 07 Oct 2018 21:24:50: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 07 Oct 2018 21:24:50: Process for pairing-model is terminated! cat: SRX3630329.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません INFO @ Sun, 07 Oct 2018 21:24:50: 50000000 pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX3630329.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3630329.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3630329.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sun, 07 Oct 2018 21:24:52: 46000000 INFO @ Sun, 07 Oct 2018 21:24:56: 51000000 INFO @ Sun, 07 Oct 2018 21:24:59: 47000000 INFO @ Sun, 07 Oct 2018 21:25:02: #1 tag size is determined as 51 bps INFO @ Sun, 07 Oct 2018 21:25:02: #1 tag size = 51 INFO @ Sun, 07 Oct 2018 21:25:02: #1 total tags in treatment: 51977848 INFO @ Sun, 07 Oct 2018 21:25:02: #1 user defined the maximum tags... INFO @ Sun, 07 Oct 2018 21:25:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 07 Oct 2018 21:25:03: #1 tags after filtering in treatment: 51977848 INFO @ Sun, 07 Oct 2018 21:25:03: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 07 Oct 2018 21:25:03: #1 finished! INFO @ Sun, 07 Oct 2018 21:25:03: #2 Build Peak Model... INFO @ Sun, 07 Oct 2018 21:25:03: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 07 Oct 2018 21:25:05: 48000000 INFO @ Sun, 07 Oct 2018 21:25:06: #2 number of paired peaks: 0 WARNING @ Sun, 07 Oct 2018 21:25:06: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 07 Oct 2018 21:25:06: Process for pairing-model is terminated! cat: SRX3630329.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX3630329.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3630329.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3630329.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sun, 07 Oct 2018 21:25:12: 49000000 INFO @ Sun, 07 Oct 2018 21:25:18: 50000000 INFO @ Sun, 07 Oct 2018 21:25:24: 51000000 INFO @ Sun, 07 Oct 2018 21:25:31: #1 tag size is determined as 51 bps INFO @ Sun, 07 Oct 2018 21:25:31: #1 tag size = 51 INFO @ Sun, 07 Oct 2018 21:25:31: #1 total tags in treatment: 51977848 INFO @ Sun, 07 Oct 2018 21:25:31: #1 user defined the maximum tags... INFO @ Sun, 07 Oct 2018 21:25:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 07 Oct 2018 21:25:32: #1 tags after filtering in treatment: 51977848 INFO @ Sun, 07 Oct 2018 21:25:32: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 07 Oct 2018 21:25:32: #1 finished! INFO @ Sun, 07 Oct 2018 21:25:32: #2 Build Peak Model... INFO @ Sun, 07 Oct 2018 21:25:32: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 07 Oct 2018 21:25:36: #2 number of paired peaks: 0 WARNING @ Sun, 07 Oct 2018 21:25:36: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 07 Oct 2018 21:25:36: Process for pairing-model is terminated! cat: SRX3630329.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX3630329.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3630329.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3630329.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。