Job ID = 11240714 sra ファイルのダウンロード中... Completed: 1356992K bytes transferred in 61 seconds (181466K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 66689191 spots for /home/okishinya/chipatlas/results/dm3/SRX3630323/SRR6652845.sra Written 66689191 spots for /home/okishinya/chipatlas/results/dm3/SRX3630323/SRR6652845.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:24:34 66689191 reads; of these: 66689191 (100.00%) were unpaired; of these: 3463420 (5.19%) aligned 0 times 50638888 (75.93%) aligned exactly 1 time 12586883 (18.87%) aligned >1 times 94.81% overall alignment rate Time searching: 00:24:34 Overall time: 00:24:34 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 28 files... [bam_rmdupse_core] 14108324 / 63225771 = 0.2231 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sun, 07 Oct 2018 21:13:42: # Command line: callpeak -t SRX3630323.bam -f BAM -g dm -n SRX3630323.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX3630323.05 # format = BAM # ChIP-seq file = ['SRX3630323.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 07 Oct 2018 21:13:42: #1 read tag files... INFO @ Sun, 07 Oct 2018 21:13:42: #1 read treatment tags... INFO @ Sun, 07 Oct 2018 21:13:42: # Command line: callpeak -t SRX3630323.bam -f BAM -g dm -n SRX3630323.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX3630323.20 # format = BAM # ChIP-seq file = ['SRX3630323.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 07 Oct 2018 21:13:42: # Command line: callpeak -t SRX3630323.bam -f BAM -g dm -n SRX3630323.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX3630323.10 # format = BAM # ChIP-seq file = ['SRX3630323.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 07 Oct 2018 21:13:42: #1 read tag files... INFO @ Sun, 07 Oct 2018 21:13:42: #1 read tag files... INFO @ Sun, 07 Oct 2018 21:13:42: #1 read treatment tags... INFO @ Sun, 07 Oct 2018 21:13:42: #1 read treatment tags... INFO @ Sun, 07 Oct 2018 21:13:48: 1000000 INFO @ Sun, 07 Oct 2018 21:13:49: 1000000 INFO @ Sun, 07 Oct 2018 21:13:49: 1000000 INFO @ Sun, 07 Oct 2018 21:13:55: 2000000 INFO @ Sun, 07 Oct 2018 21:13:55: 2000000 INFO @ Sun, 07 Oct 2018 21:13:55: 2000000 INFO @ Sun, 07 Oct 2018 21:14:02: 3000000 INFO @ Sun, 07 Oct 2018 21:14:02: 3000000 INFO @ Sun, 07 Oct 2018 21:14:02: 3000000 INFO @ Sun, 07 Oct 2018 21:14:09: 4000000 INFO @ Sun, 07 Oct 2018 21:14:09: 4000000 INFO @ Sun, 07 Oct 2018 21:14:10: 4000000 INFO @ Sun, 07 Oct 2018 21:14:15: 5000000 INFO @ Sun, 07 Oct 2018 21:14:16: 5000000 INFO @ Sun, 07 Oct 2018 21:14:17: 5000000 INFO @ Sun, 07 Oct 2018 21:14:23: 6000000 INFO @ Sun, 07 Oct 2018 21:14:24: 6000000 INFO @ Sun, 07 Oct 2018 21:14:26: 6000000 INFO @ Sun, 07 Oct 2018 21:14:30: 7000000 INFO @ Sun, 07 Oct 2018 21:14:32: 7000000 INFO @ Sun, 07 Oct 2018 21:14:34: 7000000 INFO @ Sun, 07 Oct 2018 21:14:38: 8000000 INFO @ Sun, 07 Oct 2018 21:14:40: 8000000 INFO @ Sun, 07 Oct 2018 21:14:42: 8000000 INFO @ Sun, 07 Oct 2018 21:14:46: 9000000 INFO @ Sun, 07 Oct 2018 21:14:48: 9000000 INFO @ Sun, 07 Oct 2018 21:14:50: 9000000 INFO @ Sun, 07 Oct 2018 21:14:53: 10000000 INFO @ Sun, 07 Oct 2018 21:14:56: 10000000 INFO @ Sun, 07 Oct 2018 21:14:58: 10000000 INFO @ Sun, 07 Oct 2018 21:15:01: 11000000 INFO @ Sun, 07 Oct 2018 21:15:04: 11000000 INFO @ Sun, 07 Oct 2018 21:15:07: 11000000 INFO @ Sun, 07 Oct 2018 21:15:08: 12000000 INFO @ Sun, 07 Oct 2018 21:15:12: 12000000 INFO @ Sun, 07 Oct 2018 21:15:15: 12000000 INFO @ Sun, 07 Oct 2018 21:15:15: 13000000 INFO @ Sun, 07 Oct 2018 21:15:20: 13000000 INFO @ Sun, 07 Oct 2018 21:15:23: 14000000 INFO @ Sun, 07 Oct 2018 21:15:23: 13000000 INFO @ Sun, 07 Oct 2018 21:15:28: 14000000 INFO @ Sun, 07 Oct 2018 21:15:30: 15000000 INFO @ Sun, 07 Oct 2018 21:15:31: 14000000 INFO @ Sun, 07 Oct 2018 21:15:36: 15000000 INFO @ Sun, 07 Oct 2018 21:15:37: 16000000 INFO @ Sun, 07 Oct 2018 21:15:39: 15000000 INFO @ Sun, 07 Oct 2018 21:15:43: 16000000 INFO @ Sun, 07 Oct 2018 21:15:45: 17000000 INFO @ Sun, 07 Oct 2018 21:15:47: 16000000 INFO @ Sun, 07 Oct 2018 21:15:51: 17000000 INFO @ Sun, 07 Oct 2018 21:15:52: 18000000 INFO @ Sun, 07 Oct 2018 21:15:55: 17000000 INFO @ Sun, 07 Oct 2018 21:15:59: 19000000 INFO @ Sun, 07 Oct 2018 21:15:59: 18000000 INFO @ Sun, 07 Oct 2018 21:16:04: 18000000 INFO @ Sun, 07 Oct 2018 21:16:05: 20000000 INFO @ Sun, 07 Oct 2018 21:16:07: 19000000 INFO @ Sun, 07 Oct 2018 21:16:12: 21000000 INFO @ Sun, 07 Oct 2018 21:16:12: 19000000 INFO @ Sun, 07 Oct 2018 21:16:15: 20000000 INFO @ Sun, 07 Oct 2018 21:16:18: 22000000 INFO @ Sun, 07 Oct 2018 21:16:20: 20000000 INFO @ Sun, 07 Oct 2018 21:16:23: 21000000 INFO @ Sun, 07 Oct 2018 21:16:25: 23000000 INFO @ Sun, 07 Oct 2018 21:16:28: 21000000 INFO @ Sun, 07 Oct 2018 21:16:31: 24000000 INFO @ Sun, 07 Oct 2018 21:16:31: 22000000 INFO @ Sun, 07 Oct 2018 21:16:36: 22000000 INFO @ Sun, 07 Oct 2018 21:16:38: 25000000 INFO @ Sun, 07 Oct 2018 21:16:39: 23000000 INFO @ Sun, 07 Oct 2018 21:16:44: 26000000 INFO @ Sun, 07 Oct 2018 21:16:45: 23000000 INFO @ Sun, 07 Oct 2018 21:16:47: 24000000 INFO @ Sun, 07 Oct 2018 21:16:51: 27000000 INFO @ Sun, 07 Oct 2018 21:16:53: 24000000 INFO @ Sun, 07 Oct 2018 21:16:55: 25000000 INFO @ Sun, 07 Oct 2018 21:16:57: 28000000 INFO @ Sun, 07 Oct 2018 21:17:01: 25000000 INFO @ Sun, 07 Oct 2018 21:17:03: 26000000 INFO @ Sun, 07 Oct 2018 21:17:04: 29000000 INFO @ Sun, 07 Oct 2018 21:17:09: 26000000 INFO @ Sun, 07 Oct 2018 21:17:10: 30000000 INFO @ Sun, 07 Oct 2018 21:17:11: 27000000 INFO @ Sun, 07 Oct 2018 21:17:16: 31000000 INFO @ Sun, 07 Oct 2018 21:17:17: 27000000 INFO @ Sun, 07 Oct 2018 21:17:19: 28000000 INFO @ Sun, 07 Oct 2018 21:17:23: 32000000 INFO @ Sun, 07 Oct 2018 21:17:25: 28000000 INFO @ Sun, 07 Oct 2018 21:17:27: 29000000 INFO @ Sun, 07 Oct 2018 21:17:30: 33000000 INFO @ Sun, 07 Oct 2018 21:17:34: 29000000 INFO @ Sun, 07 Oct 2018 21:17:35: 30000000 INFO @ Sun, 07 Oct 2018 21:17:37: 34000000 INFO @ Sun, 07 Oct 2018 21:17:42: 30000000 INFO @ Sun, 07 Oct 2018 21:17:43: 31000000 INFO @ Sun, 07 Oct 2018 21:17:44: 35000000 INFO @ Sun, 07 Oct 2018 21:17:50: 31000000 INFO @ Sun, 07 Oct 2018 21:17:50: 36000000 INFO @ Sun, 07 Oct 2018 21:17:51: 32000000 INFO @ Sun, 07 Oct 2018 21:17:57: 37000000 INFO @ Sun, 07 Oct 2018 21:17:58: 32000000 INFO @ Sun, 07 Oct 2018 21:17:59: 33000000 INFO @ Sun, 07 Oct 2018 21:18:04: 38000000 INFO @ Sun, 07 Oct 2018 21:18:06: 33000000 INFO @ Sun, 07 Oct 2018 21:18:06: 34000000 INFO @ Sun, 07 Oct 2018 21:18:12: 39000000 INFO @ Sun, 07 Oct 2018 21:18:14: 34000000 INFO @ Sun, 07 Oct 2018 21:18:14: 35000000 INFO @ Sun, 07 Oct 2018 21:18:19: 40000000 INFO @ Sun, 07 Oct 2018 21:18:22: 35000000 INFO @ Sun, 07 Oct 2018 21:18:22: 36000000 INFO @ Sun, 07 Oct 2018 21:18:27: 41000000 INFO @ Sun, 07 Oct 2018 21:18:30: 37000000 INFO @ Sun, 07 Oct 2018 21:18:30: 36000000 INFO @ Sun, 07 Oct 2018 21:18:35: 42000000 INFO @ Sun, 07 Oct 2018 21:18:38: 38000000 INFO @ Sun, 07 Oct 2018 21:18:39: 37000000 INFO @ Sun, 07 Oct 2018 21:18:43: 43000000 INFO @ Sun, 07 Oct 2018 21:18:46: 39000000 INFO @ Sun, 07 Oct 2018 21:18:47: 38000000 INFO @ Sun, 07 Oct 2018 21:18:51: 44000000 INFO @ Sun, 07 Oct 2018 21:18:54: 40000000 INFO @ Sun, 07 Oct 2018 21:18:55: 39000000 INFO @ Sun, 07 Oct 2018 21:18:58: 45000000 INFO @ Sun, 07 Oct 2018 21:19:02: 41000000 INFO @ Sun, 07 Oct 2018 21:19:03: 40000000 INFO @ Sun, 07 Oct 2018 21:19:06: 46000000 INFO @ Sun, 07 Oct 2018 21:19:10: 42000000 INFO @ Sun, 07 Oct 2018 21:19:11: 41000000 INFO @ Sun, 07 Oct 2018 21:19:14: 47000000 INFO @ Sun, 07 Oct 2018 21:19:18: 43000000 INFO @ Sun, 07 Oct 2018 21:19:20: 42000000 INFO @ Sun, 07 Oct 2018 21:19:21: 48000000 INFO @ Sun, 07 Oct 2018 21:19:26: 44000000 INFO @ Sun, 07 Oct 2018 21:19:28: 43000000 INFO @ Sun, 07 Oct 2018 21:19:29: 49000000 INFO @ Sun, 07 Oct 2018 21:19:30: #1 tag size is determined as 51 bps INFO @ Sun, 07 Oct 2018 21:19:30: #1 tag size = 51 INFO @ Sun, 07 Oct 2018 21:19:30: #1 total tags in treatment: 49117447 INFO @ Sun, 07 Oct 2018 21:19:30: #1 user defined the maximum tags... INFO @ Sun, 07 Oct 2018 21:19:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 07 Oct 2018 21:19:31: #1 tags after filtering in treatment: 49117447 INFO @ Sun, 07 Oct 2018 21:19:31: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 07 Oct 2018 21:19:31: #1 finished! INFO @ Sun, 07 Oct 2018 21:19:31: #2 Build Peak Model... INFO @ Sun, 07 Oct 2018 21:19:31: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 07 Oct 2018 21:19:34: 45000000 INFO @ Sun, 07 Oct 2018 21:19:34: #2 number of paired peaks: 0 WARNING @ Sun, 07 Oct 2018 21:19:34: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 07 Oct 2018 21:19:34: Process for pairing-model is terminated! cat: SRX3630323.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX3630323.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3630323.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3630323.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sun, 07 Oct 2018 21:19:36: 44000000 INFO @ Sun, 07 Oct 2018 21:19:41: 46000000 INFO @ Sun, 07 Oct 2018 21:19:44: 45000000 INFO @ Sun, 07 Oct 2018 21:19:49: 47000000 INFO @ Sun, 07 Oct 2018 21:19:52: 46000000 INFO @ Sun, 07 Oct 2018 21:19:57: 48000000 INFO @ Sun, 07 Oct 2018 21:20:00: 47000000 INFO @ Sun, 07 Oct 2018 21:20:05: 49000000 INFO @ Sun, 07 Oct 2018 21:20:06: #1 tag size is determined as 51 bps INFO @ Sun, 07 Oct 2018 21:20:06: #1 tag size = 51 INFO @ Sun, 07 Oct 2018 21:20:06: #1 total tags in treatment: 49117447 INFO @ Sun, 07 Oct 2018 21:20:06: #1 user defined the maximum tags... INFO @ Sun, 07 Oct 2018 21:20:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 07 Oct 2018 21:20:07: #1 tags after filtering in treatment: 49117447 INFO @ Sun, 07 Oct 2018 21:20:07: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 07 Oct 2018 21:20:07: #1 finished! INFO @ Sun, 07 Oct 2018 21:20:07: #2 Build Peak Model... INFO @ Sun, 07 Oct 2018 21:20:07: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 07 Oct 2018 21:20:08: 48000000 INFO @ Sun, 07 Oct 2018 21:20:11: #2 number of paired peaks: 0 WARNING @ Sun, 07 Oct 2018 21:20:11: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 07 Oct 2018 21:20:11: Process for pairing-model is terminated! cat: SRX3630323.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX3630323.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3630323.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3630323.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sun, 07 Oct 2018 21:20:15: 49000000 INFO @ Sun, 07 Oct 2018 21:20:17: #1 tag size is determined as 51 bps INFO @ Sun, 07 Oct 2018 21:20:17: #1 tag size = 51 INFO @ Sun, 07 Oct 2018 21:20:17: #1 total tags in treatment: 49117447 INFO @ Sun, 07 Oct 2018 21:20:17: #1 user defined the maximum tags... INFO @ Sun, 07 Oct 2018 21:20:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 07 Oct 2018 21:20:18: #1 tags after filtering in treatment: 49117447 INFO @ Sun, 07 Oct 2018 21:20:18: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 07 Oct 2018 21:20:18: #1 finished! INFO @ Sun, 07 Oct 2018 21:20:18: #2 Build Peak Model... INFO @ Sun, 07 Oct 2018 21:20:18: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 07 Oct 2018 21:20:21: #2 number of paired peaks: 0 WARNING @ Sun, 07 Oct 2018 21:20:21: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 07 Oct 2018 21:20:21: Process for pairing-model is terminated! cat: SRX3630323.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX3630323.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3630323.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3630323.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。