Job ID = 6527967 SRX = SRX3511950 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T13:47:12 prefetch.2.10.7: 1) Downloading 'SRR6418935'... 2020-06-29T13:47:12 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T13:49:57 prefetch.2.10.7: HTTPS download succeed 2020-06-29T13:49:57 prefetch.2.10.7: 1) 'SRR6418935' was downloaded successfully 2020-06-29T13:49:57 prefetch.2.10.7: 'SRR6418935' has 0 unresolved dependencies Read 11872467 spots for SRR6418935/SRR6418935.sra Written 11872467 spots for SRR6418935/SRR6418935.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:56 11872467 reads; of these: 11872467 (100.00%) were unpaired; of these: 1275767 (10.75%) aligned 0 times 8760053 (73.78%) aligned exactly 1 time 1836647 (15.47%) aligned >1 times 89.25% overall alignment rate Time searching: 00:05:56 Overall time: 00:05:56 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 380605 / 10596700 = 0.0359 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:09:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3511950/SRX3511950.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3511950/SRX3511950.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3511950/SRX3511950.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3511950/SRX3511950.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:09:37: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:09:37: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:09:44: 1000000 INFO @ Mon, 29 Jun 2020 23:09:51: 2000000 INFO @ Mon, 29 Jun 2020 23:09:58: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:10:05: 4000000 INFO @ Mon, 29 Jun 2020 23:10:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3511950/SRX3511950.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3511950/SRX3511950.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3511950/SRX3511950.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3511950/SRX3511950.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:10:07: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:10:07: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:10:13: 5000000 INFO @ Mon, 29 Jun 2020 23:10:14: 1000000 INFO @ Mon, 29 Jun 2020 23:10:21: 6000000 INFO @ Mon, 29 Jun 2020 23:10:22: 2000000 INFO @ Mon, 29 Jun 2020 23:10:28: 7000000 INFO @ Mon, 29 Jun 2020 23:10:29: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:10:36: 8000000 INFO @ Mon, 29 Jun 2020 23:10:37: 4000000 INFO @ Mon, 29 Jun 2020 23:10:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3511950/SRX3511950.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3511950/SRX3511950.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3511950/SRX3511950.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3511950/SRX3511950.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:10:37: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:10:37: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:10:44: 9000000 INFO @ Mon, 29 Jun 2020 23:10:44: 5000000 INFO @ Mon, 29 Jun 2020 23:10:45: 1000000 INFO @ Mon, 29 Jun 2020 23:10:52: 10000000 INFO @ Mon, 29 Jun 2020 23:10:52: 6000000 INFO @ Mon, 29 Jun 2020 23:10:52: 2000000 INFO @ Mon, 29 Jun 2020 23:10:53: #1 tag size is determined as 100 bps INFO @ Mon, 29 Jun 2020 23:10:53: #1 tag size = 100 INFO @ Mon, 29 Jun 2020 23:10:53: #1 total tags in treatment: 10216095 INFO @ Mon, 29 Jun 2020 23:10:53: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:10:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:10:53: #1 tags after filtering in treatment: 10216095 INFO @ Mon, 29 Jun 2020 23:10:53: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:10:53: #1 finished! INFO @ Mon, 29 Jun 2020 23:10:53: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:10:53: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:10:54: #2 number of paired peaks: 48 WARNING @ Mon, 29 Jun 2020 23:10:54: Too few paired peaks (48) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:10:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3511950/SRX3511950.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3511950/SRX3511950.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3511950/SRX3511950.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3511950/SRX3511950.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:10:59: 7000000 INFO @ Mon, 29 Jun 2020 23:11:00: 3000000 INFO @ Mon, 29 Jun 2020 23:11:07: 8000000 INFO @ Mon, 29 Jun 2020 23:11:07: 4000000 INFO @ Mon, 29 Jun 2020 23:11:14: 9000000 INFO @ Mon, 29 Jun 2020 23:11:15: 5000000 INFO @ Mon, 29 Jun 2020 23:11:22: 10000000 INFO @ Mon, 29 Jun 2020 23:11:22: 6000000 INFO @ Mon, 29 Jun 2020 23:11:23: #1 tag size is determined as 100 bps INFO @ Mon, 29 Jun 2020 23:11:23: #1 tag size = 100 INFO @ Mon, 29 Jun 2020 23:11:23: #1 total tags in treatment: 10216095 INFO @ Mon, 29 Jun 2020 23:11:23: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:11:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:11:24: #1 tags after filtering in treatment: 10216095 INFO @ Mon, 29 Jun 2020 23:11:24: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:11:24: #1 finished! INFO @ Mon, 29 Jun 2020 23:11:24: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:11:24: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:11:24: #2 number of paired peaks: 48 WARNING @ Mon, 29 Jun 2020 23:11:24: Too few paired peaks (48) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:11:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3511950/SRX3511950.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3511950/SRX3511950.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3511950/SRX3511950.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3511950/SRX3511950.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:11:29: 7000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 23:11:37: 8000000 INFO @ Mon, 29 Jun 2020 23:11:44: 9000000 INFO @ Mon, 29 Jun 2020 23:11:51: 10000000 INFO @ Mon, 29 Jun 2020 23:11:53: #1 tag size is determined as 100 bps INFO @ Mon, 29 Jun 2020 23:11:53: #1 tag size = 100 INFO @ Mon, 29 Jun 2020 23:11:53: #1 total tags in treatment: 10216095 INFO @ Mon, 29 Jun 2020 23:11:53: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:11:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:11:53: #1 tags after filtering in treatment: 10216095 INFO @ Mon, 29 Jun 2020 23:11:53: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:11:53: #1 finished! INFO @ Mon, 29 Jun 2020 23:11:53: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:11:53: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:11:54: #2 number of paired peaks: 48 WARNING @ Mon, 29 Jun 2020 23:11:54: Too few paired peaks (48) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:11:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3511950/SRX3511950.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3511950/SRX3511950.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3511950/SRX3511950.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3511950/SRX3511950.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。